Standard name
Human Ortholog
Description Ribosomal 60S subunit protein L13B; not essential for viability; homologous to mammalian ribosomal protein L13, no bacterial homolog; RPL13B has a paralog, RPL13A, that arose from the whole genome duplication
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0.07 0.07 0.06 0.11 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0.07 0.11 0.08 0.09 0.1 0.12 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.97 0.98 0.99 0.99 0.98 0.97 0.75 0.66 0.71 0.66 0.69 0.65 0.99 0.98 0.96 0.99 0.99 1.0 0.96 0.91 0.96 0.95 0.92 0.92
Endoplasmic Reticulum 0 0 0 0 0 0 0.2 0.2 0.16 0.23 0.17 0.17 0 0 0 0.05 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0.06 0.06 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 0 0.07 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 12 7 21 18 12 28 0 0 0 0 0 0 0 0 0 0 1 1
Bud 0 0 1 0 0 0 1 1 1 5 1 1 0 2 2 0 0 0 0 0 1 1 4 4
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 2 0 4 9 8 8 28 32 24 24 19 33 1 11 16 7 1 4 0 0 0 0 0 0
Cytoplasm 195 95 152 202 280 280 293 192 208 173 131 174 247 322 414 349 136 258 188 101 139 250 314 419
Endoplasmic Reticulum 2 1 0 4 7 5 78 57 48 59 32 46 4 1 9 18 6 7 2 1 1 6 10 13
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 1 4 6 17 15 1 9 0 1 1 0 0 1 0 0 0 0 0 1
Mitochondria 1 1 0 0 0 0 2 1 11 6 14 9 0 0 0 0 1 0 1 0 0 0 1 1
Nucleus 1 0 0 0 0 0 0 2 0 0 1 0 0 1 0 0 0 0 0 1 1 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 1 0 0 0 1 15 6 1 0 2 0 1 3 0 0 2 0 2 1 1 1 4
Unique Cell Count 200 97 154 205 287 288 392 290 291 262 190 266 250 327 431 352 137 259 197 112 145 262 341 455
Labelled Cell Count 201 97 158 215 295 294 419 313 336 301 211 302 252 339 445 374 144 272 197 112 145 262 341 455


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 78.8 101.5 92.3 88.5 68.7 68.7 41.8 37.6 32.7 31.3 27.6 28.2 105.9 101.5 92.9 164.2 152.6 164.9 102.3 100.0 104.9
Std Deviation (1e-4) 15.9 24.6 20.0 18.6 14.9 14.9 11.2 9.9 9.8 8.4 8.7 6.5 22.9 23.5 21.5 41.1 52.0 53.2 23.3 26.0 27.5
Intensity Change (Log2) – – – -0.06 -0.43 -0.43 -1.14 -1.3 -1.5 -1.56 -1.74 -1.71 0.2 0.14 0.01 0.83 0.73 0.84 0.15 0.12 0.19


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 3.4 3.3 3.2 4.2 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0.9 0 0 2.0 3.1 2.3 2.6 2.7 3.4 0 0 0.7 0 0 0
Cytoplasm -0.1 -0.8 -1.0 -6.5 -7.8 -6.9 -7.8 -7.2 -7.9 0.1 -0.2 -1.6 0.5 0.5 1.1
Endoplasmic Reticulum 0 0 0 6.0 5.9 5.3 6.4 5.3 5.5 0 0 0 2.9 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 3.1 3.0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 3.4 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 2.4 0 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 77.9259 113.8093 106.4869 85.8545 63.4818 104.6679 106.9876 131.3893 126.1451 120.9801 116.4413 126.9823 90.7897 137.0969 119.9924 103.6933 122.8609 121.9423
Actin 0.0014 0.0003 0.0203 0.0076 0.0021 0.0011 0.001 0.0003 0.0003 0.0001 0.0009 0.0005 0.048 0.0003 0.0103 0.0019 0.0004 0.0139
Bud 0.0014 0.0007 0.0009 0.0025 0.0038 0.0008 0.0007 0.0004 0.0004 0.001 0.0002 0.0007 0.002 0.0004 0.0014 0.0007 0.0018 0.0019
Bud Neck 0.0005 0.0004 0.0014 0.0225 0.0004 0.0012 0.0005 0.0004 0.0009 0.0006 0.0009 0.0012 0.0014 0.0003 0.0004 0.0006 0.0008 0.0015
Bud Periphery 0.0038 0.0004 0.0012 0.0072 0.0252 0.0005 0.0008 0.0002 0.0002 0.0003 0.0001 0.0001 0.0022 0.0002 0.0028 0.0003 0.0003 0.0017
Bud Site 0.0004 0.0008 0.0039 0.0086 0.0005 0.0001 0.0003 0.0003 0.0002 0.0002 0 0.0001 0.0034 0.0003 0.0018 0.0006 0.0001 0.0045
Cell Periphery 0.021 0.0136 0.0041 0.0156 0.006 0.001 0.0076 0.0039 0.0083 0.0086 0.0003 0.0005 0.0123 0.0131 0.0041 0.0007 0.0018 0.0005
Cytoplasm 0.687 0.9282 0.8563 0.6393 0.59 0.894 0.8057 0.9582 0.9078 0.7503 0.9308 0.876 0.6526 0.9473 0.8722 0.7293 0.8976 0.853
Cytoplasmic Foci 0.0055 0.0017 0.0138 0.0108 0.0445 0.0033 0.0021 0.0006 0.0011 0.0014 0.0023 0.0031 0.0318 0.0011 0.0057 0.0678 0.0022 0.008
Eisosomes 0.0001 0 0.0003 0.0002 0.0001 0 0.0002 0.0001 0 0 0 0 0.0012 0 0.0001 0.0004 0 0.0001
Endoplasmic Reticulum 0.071 0.0109 0.0253 0.0088 0.0408 0.0613 0.0323 0.0042 0.0092 0.0025 0.0072 0.0817 0.0229 0.0036 0.0085 0.0119 0.0007 0.0291
Endosome 0.0321 0.0018 0.02 0.0276 0.031 0.0098 0.0079 0.0008 0.0022 0.0068 0.0042 0.0062 0.0266 0.001 0.0062 0.0079 0.0064 0.0306
Golgi 0.0013 0.0002 0.0077 0.016 0.0031 0.0018 0.0011 0.0002 0.0002 0.0002 0.0003 0.0007 0.0095 0.0002 0.0016 0.0021 0.0004 0.0128
Lipid Particles 0.0027 0.0008 0.004 0.0053 0.0148 0.0001 0.0016 0.0005 0.0007 0.0006 0.0001 0.0006 0.0158 0.0021 0.0014 0.0432 0.0004 0.0015
Mitochondria 0.0023 0.0003 0.002 0.0324 0.0038 0.0027 0.005 0.0004 0.0001 0.0002 0.0001 0.0005 0.0128 0.0007 0.0057 0.0089 0.0004 0.012
None 0.007 0.0006 0.0006 0.0061 0.0217 0.0007 0.0109 0.0004 0.0002 0.0002 0.0001 0.0004 0.0085 0.0021 0.0039 0.0422 0.0003 0.0007
Nuclear Periphery 0.0374 0.0053 0.0038 0.0085 0.0263 0.0055 0.0234 0.0027 0.0067 0.0075 0.0175 0.013 0.0286 0.0036 0.0083 0.0081 0.0041 0.0055
Nucleolus 0.0019 0.0007 0.0002 0.0028 0.0021 0 0.005 0.0007 0.0001 0.0014 0 0.0001 0.0028 0.0003 0.0043 0.0022 0.0002 0.0001
Nucleus 0.0109 0.0022 0.002 0.0032 0.0067 0.003 0.0101 0.0019 0.0028 0.007 0.0039 0.0029 0.0133 0.0018 0.0031 0.0035 0.0042 0.0029
Peroxisomes 0.0003 0 0.0036 0.0018 0.0019 0.0001 0.0001 0 0 0 0 0 0.0111 0 0.0005 0.0029 0.0001 0.0008
Punctate Nuclear 0.0032 0.0001 0.0008 0.0003 0.0046 0.0001 0.0034 0 0 0 0.0001 0.0002 0.0078 0.0001 0.0004 0.0053 0.0001 0.0002
Vacuole 0.094 0.0272 0.0244 0.1294 0.128 0.0096 0.0581 0.0222 0.053 0.1756 0.0258 0.0097 0.0638 0.02 0.0481 0.0457 0.0624 0.0116
Vacuole Periphery 0.0148 0.0037 0.0033 0.0436 0.0426 0.0032 0.022 0.0015 0.0055 0.0353 0.0052 0.0022 0.0216 0.0016 0.0093 0.0137 0.0154 0.007

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 2617.827 2839.1194 3298.5811 2466.7135 3081.7769 2780.8779 2422.2505 2129.8842 2526.3253 2045.5608
Translational Efficiency 1.6322 1.6485 1.4668 1.971 1.8193 1.5306 1.4728 1.9047 1.3449 1.7354

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Ribosomal 60S subunit protein L13B; not essential for viability; homologous to mammalian ribosomal protein L13, no bacterial homolog; RPL13B has a paralog, RPL13A, that arose from the whole genome duplication
Localization
Cell Percentages cytoplasm (100%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-2

Rpl13b

Rpl13b


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Rpl13b-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available