Standard name
Human Ortholog
Description Protein of unknown function; null mutant displays sensitivity to DNA damaging agents; may have a role in lipid metabolism, based on localization to lipid droplets; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; protein abundance increases in response to DNA replication stress

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.1 0.08 0 0 0 0 0 0
Cytoplasm 0.38 0.36 0.28 0.34 0.39 0.31 0.23 0.16 0.18 0.13 0.14 0.12 0.32 0.2 0.25 0.09 0.1 0.06 0.08 0.07 0.06
Endoplasmic Reticulum 0.75 0.7 0.69 0.54 0.42 0.41 0.22 0.19 0.2 0.14 0.16 0.18 0.65 0.75 0.71 0.81 0.79 0.84 0.75 0.74 0.73
Endosome 0 0 0 0 0 0 0.05 0.05 0 0.06 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0.09 0.16 0.17 0.28 0.36 0.42 0.42 0.4 0.6 0.63 0 0 0 0 0 0 0.05 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0.05 0.12 0.15 0.31 0.31 0.28 0.36 0.2 0.15 0.07 0 0 0 0 0 0.05 0.07 0.07
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 6 8 8 9 13 9 7 19 13 14 13 18 9 13 0 1 6 6 6 9
Bud 0 0 3 2 5 17 30 29 22 20 25 18 2 0 0 0 0 0 2 4 3
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 2 3 3 3 6
Bud Site 0 0 0 0 0 0 0 3 0 4 2 3 0 0 0
Cell Periphery 0 6 5 10 16 9 13 4 8 9 5 5 21 26 34 0 0 2 2 4 7
Cytoplasm 6 52 76 83 152 133 168 93 123 95 86 84 126 52 104 3 17 19 27 24 21
Endoplasmic Reticulum 12 100 185 130 161 175 160 114 134 99 99 129 257 196 293 30 136 282 255 256 253
Endosome 0 0 0 7 7 4 34 32 26 45 10 14 8 3 6 0 2 0 1 3 2
Golgi 0 1 1 0 0 0 0 1 1 5 3 5 1 1 6 0 0 0 2 4 5
Mitochondria 0 0 23 39 67 118 265 248 286 291 370 458 2 0 0 0 0 9 16 11 9
Nucleus 0 0 0 1 0 2 8 4 5 9 9 13 0 1 0 0 1 0 0 0 0
Nuclear Periphery 0 0 1 1 1 3 7 9 12 10 19 13 0 0 0 0 0 1 0 0 2
Nucleolus 0 0 0 0 1 0 7 11 6 13 8 22 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 1 0 0 0 1 0 6 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 6 3 11 45 62 225 188 187 262 121 109 29 8 12 0 6 5 18 23 23
Unique Cell Count 16 143 270 241 387 424 736 597 674 721 617 730 397 263 412 38 173 335 340 348 347
Labelled Cell Count 18 171 305 293 464 536 926 744 829 882 771 886 464 296 468 38 173 335 340 348 347


Endoplasmic Reticulum

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 8.9 11.6 7.3 7.4 7.3 6.9 6.7 7.1 7.4 7.3 7.1 7.3 10.1 10.8 11.7 10.8 10.9 11.4
Std Deviation (1e-4) 1.9 1.8 1.1 1.3 1.3 1.1 1.3 1.5 1.6 1.6 1.8 1.8 1.3 1.5 1.7 2.2 2.0 2.0
Intensity Change (Log2) 0.02 -0.0 -0.09 -0.12 -0.05 0.02 0.0 -0.03 -0.01 0.48 0.56 0.68 0.57 0.57 0.65


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0.2 -0.5 0.1 0 0 -0.1 -1.1 -0.6 -1.2 1.0 0.3 0.1
Bud 0 0 2.2 2.3 2.7 1.9 1.5 2.3 1.3 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 1.5 1.6 0.2 0 0 0 0 0 0 2.3 4.0 3.5
Cytoplasm 1.5 2.9 0.9 -1.7 -4.3 -3.4 -5.6 -5.0 -6.4 1.0 -2.3 -0.8
Endoplasmic Reticulum -3.4 -6.8 -7.0 -13.9 -14.2 -14.3 -17.0 -15.4 -15.4 -1.0 1.5 0.7
Endosome 0 0 0 3.6 3.9 3.3 4.2 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 2.6 3.2 6.2 8.5 9.7 10.0 9.6 14.2 15.2 -5.3 -4.8 -6.0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 2.5 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 2.9 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 2.4 5.1 6.0 9.9 10.0 9.2 11.2 7.3 6.2 3.7 1.6 1.6

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 4.511 5.506 4.5931 4.0009 3.9821 4.875 6.2524 7.3067 6.6958 6.3541 5.0964 6.6202 5.465 5.8242 5.1415 5.7688 4.8014 5.4203
Actin 0.0099 0.0013 0.0128 0.0033 0.0126 0.0039 0.0144 0.0103 0.0128 0.004 0.0045 0.0039 0.0112 0.0016 0.0068 0.0007 0.0023 0.0008
Bud 0.0073 0.0016 0.0056 0.0002 0.0008 0.0027 0.0002 0.0001 0.0011 0.0004 0.0001 0.0001 0.0001 0 0.0001 0.0001 0.0002 0
Bud Neck 0.0006 0.001 0.0019 0.0007 0.0013 0.0016 0.0006 0.0006 0.0051 0.0014 0.0012 0.0012 0.0003 0.0004 0.0006 0.0006 0.0006 0.0009
Bud Periphery 0.0022 0.001 0.0022 0.0006 0.0017 0.0007 0.0002 0.0002 0.0029 0.0012 0.0002 0.0002 0.0001 0.0001 0.0001 0.0004 0.0002 0.0001
Bud Site 0.0021 0.0195 0.0129 0.0005 0.0019 0.0006 0.0015 0.0022 0.0051 0.001 0.0002 0.0002 0.0003 0.0015 0.0005 0.0001 0.0004 0.0001
Cell Periphery 0.0016 0.0035 0.0031 0.0012 0.0034 0.0015 0.0019 0.0057 0.0054 0.003 0.0035 0.0025 0.0013 0.0016 0.0016 0.0022 0.0008 0.001
Cytoplasm 0.1168 0.2307 0.2057 0.1535 0.2038 0.2046 0.1053 0.1614 0.1248 0.1821 0.1908 0.1384 0.0474 0.09 0.093 0.0382 0.221 0.0888
Cytoplasmic Foci 0.0446 0.0127 0.0285 0.0148 0.0447 0.0171 0.0284 0.0139 0.0199 0.0194 0.0141 0.0145 0.0256 0.02 0.0218 0.0283 0.0274 0.014
Eisosomes 0.0001 0.0001 0.0001 0.0003 0.0003 0.0001 0.0003 0.0018 0.0004 0.0001 0.0002 0.0001 0.0001 0 0.0001 0.0001 0 0
Endoplasmic Reticulum 0.5291 0.589 0.5244 0.6066 0.3839 0.6384 0.6253 0.6377 0.6419 0.654 0.6854 0.7085 0.6352 0.6683 0.6596 0.6783 0.5 0.6889
Endosome 0.1168 0.0492 0.0881 0.0718 0.1154 0.0544 0.0742 0.0414 0.0674 0.0518 0.0305 0.0423 0.0779 0.0479 0.059 0.0692 0.1218 0.0748
Golgi 0.033 0.008 0.0199 0.0106 0.0329 0.0103 0.0291 0.0099 0.0195 0.0225 0.0079 0.0159 0.016 0.0087 0.0153 0.0411 0.0131 0.0135
Lipid Particles 0.0518 0.0284 0.0418 0.0592 0.0487 0.0276 0.0746 0.0842 0.0542 0.0211 0.0404 0.041 0.1473 0.1348 0.1157 0.1139 0.0847 0.0918
Mitochondria 0.0078 0.0013 0.0119 0.0224 0.0412 0.0019 0.004 0.0014 0.0115 0.006 0.0016 0.0056 0.001 0.0004 0.0012 0.0016 0.0056 0.0016
None 0.013 0.0166 0.0113 0.0102 0.0622 0.0128 0.0073 0.0065 0.0013 0.0014 0.0082 0.0091 0.0084 0.0017 0.0011 0.001 0.0017 0.0011
Nuclear Periphery 0.0219 0.0135 0.0098 0.0258 0.0117 0.0086 0.0097 0.0082 0.007 0.009 0.005 0.0048 0.0142 0.0118 0.0123 0.0082 0.0072 0.0102
Nucleolus 0.0011 0.0005 0.0004 0.0003 0.001 0.0002 0.0003 0.0001 0.0002 0.0001 0 0.0001 0.0001 0.0001 0 0 0.0001 0
Nucleus 0.008 0.0055 0.0028 0.0016 0.0041 0.0026 0.0011 0.0011 0.0011 0.0014 0.0007 0.0006 0.0006 0.0014 0.0008 0.0005 0.0022 0.0013
Peroxisomes 0.011 0.0002 0.0023 0.0037 0.0052 0.0003 0.01 0.0004 0.0036 0.004 0.0005 0.0024 0.0013 0.0004 0.0005 0.0002 0.0003 0.0002
Punctate Nuclear 0.0077 0.002 0.0029 0.0007 0.0039 0.001 0.0031 0.0014 0.0006 0.0004 0.0004 0.0004 0.0012 0.0012 0.0007 0.0011 0.0012 0.0004
Vacuole 0.0112 0.0132 0.0095 0.0095 0.0158 0.0081 0.0074 0.0107 0.0127 0.0138 0.004 0.0073 0.0094 0.0078 0.0082 0.0125 0.0079 0.0091
Vacuole Periphery 0.0023 0.001 0.0018 0.0022 0.0035 0.001 0.001 0.0008 0.0018 0.0021 0.0006 0.0009 0.0011 0.0006 0.001 0.0015 0.0012 0.0014

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 36.1772 28.7468 27.3309 41.1241 26.0585 45.1965 33.1533 39.8481 41.2808 35.4753
Translational Efficiency 1.32 1.1784 1.4106 0.9889 1.2013 1.1711 1.286 0.9651 1.1855 1.1024

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency
Standard name
Human Ortholog
Description Protein of unknown function; null mutant displays sensitivity to DNA damaging agents; may have a role in lipid metabolism, based on localization to lipid droplets; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; protein abundance increases in response to DNA replication stress
Localization
Cell Percentages ER (91%)
Cell Cycle Regulation No
Subcompartmental Group ER-3

Yim1

Yim1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)

Molecular Function (Gene Ontology)

Cellular Component (Gene Ontology)


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Yim1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available