Standard name | |
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Human Ortholog | |
Description | FG-nucleoporin component of central core of nuclear pore complex (NPC); also part of the NPC nuclear basket; contributes directly to nucleocytoplasmic transport; involved in regulation of transcription and mitosis; induces membrane tubulation, which may contribute to nuclear pore assembly; NUP53 has a paralog, ASM4, that arose from the whole genome duplication |
ensLOC | DeepLoc | |||||||||||||||||||||||
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Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 3 | 0 | 0 | 0 | 2 | 0 | 1 | 0 | 5 | 3 |
Bud | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 2 | 4 | 2 | 1 | 2 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 2 | 2 | 3 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 1 |
Bud Site | 0 | 0 | 0 | 0 | 2 | 1 | 3 | 5 | 2 | 1 | 5 | 1 | 2 | 2 | 3 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 3 | 2 | 9 | 2 | 13 | 10 | 14 | 11 | 6 | 11 | 9 | 7 | 38 | 38 | 54 | 8 | 12 | 5 | 1 | 0 | 0 | 2 | 1 | 1 |
Endoplasmic Reticulum | 0 | 1 | 4 | 1 | 1 | 3 | 2 | 3 | 1 | 1 | 2 | 4 | 2 | 6 | 4 | 1 | 3 | 2 | 0 | 0 | 0 | 2 | 3 | 2 |
Endosome | 3 | 0 | 13 | 9 | 16 | 15 | 12 | 25 | 3 | 4 | 1 | 8 | 2 | 2 | 6 | 3 | 4 | 2 | 5 | 1 | 5 | 7 | 20 | 26 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 1 | 1 | 0 | 3 | 8 | 9 |
Mitochondria | 8 | 15 | 38 | 35 | 31 | 139 | 172 | 173 | 127 | 186 | 203 | 166 | 4 | 4 | 4 | 10 | 21 | 5 | 5 | 2 | 7 | 29 | 56 | 82 |
Nucleus | 18 | 6 | 5 | 6 | 9 | 16 | 18 | 22 | 12 | 17 | 26 | 14 | 32 | 32 | 20 | 7 | 9 | 3 | 4 | 3 | 3 | 1 | 5 | 4 |
Nuclear Periphery | 202 | 91 | 308 | 214 | 213 | 346 | 383 | 487 | 228 | 278 | 300 | 342 | 307 | 311 | 292 | 105 | 115 | 71 | 257 | 91 | 346 | 276 | 455 | 496 |
Nucleolus | 11 | 4 | 47 | 65 | 37 | 114 | 134 | 209 | 117 | 151 | 160 | 199 | 14 | 14 | 6 | 7 | 19 | 8 | 2 | 0 | 1 | 2 | 3 | 5 |
Peroxisomes | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 0 | 1 | 1 | 1 | 0 | 0 | 6 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 4 | 9 |
SpindlePole | 0 | 1 | 0 | 0 | 1 | 2 | 0 | 5 | 1 | 2 | 2 | 1 | 7 | 8 | 18 | 0 | 2 | 0 | 2 | 0 | 1 | 2 | 1 | 2 |
Vac/Vac Membrane | 88 | 26 | 60 | 25 | 45 | 48 | 33 | 40 | 24 | 24 | 16 | 26 | 102 | 104 | 142 | 56 | 59 | 36 | 10 | 6 | 5 | 9 | 16 | 23 |
Unique Cell Count | 257 | 108 | 354 | 254 | 285 | 501 | 551 | 704 | 373 | 469 | 490 | 514 | 410 | 415 | 430 | 150 | 181 | 94 | 296 | 110 | 376 | 343 | 586 | 678 |
Labelled Cell Count | 333 | 146 | 484 | 358 | 370 | 695 | 772 | 983 | 525 | 679 | 726 | 771 | 510 | 521 | 558 | 197 | 247 | 133 | 296 | 110 | 376 | 343 | 586 | 678 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 10.2 | 9.1 | 9.7 | 9.5 | 8.9 | 7.9 | 7.8 | 8.2 | 7.7 | 7.5 | 7.6 | 8.3 | 8.9 | 8.9 | 9.1 | 9.4 | 10.3 | 13.0 | 9.2 | 10.3 | 10.5 |
Std Deviation (1e-4) | 2.2 | 1.9 | 2.5 | 2.8 | 2.4 | 2.1 | 2.1 | 2.1 | 2.3 | 2.4 | 2.3 | 2.9 | 2.1 | 2.1 | 2.4 | 2.0 | 3.0 | 3.6 | 1.9 | 1.8 | 1.8 |
Intensity Change (Log2) | – | – | – | -0.02 | -0.11 | -0.29 | -0.31 | -0.24 | -0.33 | -0.37 | -0.34 | -0.23 | -0.12 | -0.12 | -0.09 | -0.04 | 0.1 | 0.43 | -0.08 | 0.09 | 0.12 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 0 | 1.4 | -0.5 | 0 | -1.1 | -0.9 | -0.2 | -0.7 | -1.3 | 3.9 | 3.8 | 5.1 | 1.6 | 2.3 | 0 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | -0.1 | 1.2 | -0.5 | -1.3 | -0.1 | -2.6 | -2.8 | -3.9 | -2.0 | -3.2 | -3.2 | -2.1 | 0 | -0.9 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 1.1 | 0.1 | 6.0 | 7.1 | 5.3 | 7.5 | 9.2 | 9.7 | 7.4 | -5.9 | -5.9 | -6.1 | -1.4 | 0.3 | -1.6 |
Nucleus | 0 | 1.5 | 1.7 | 1.7 | 1.7 | 1.6 | 1.9 | 3.0 | 1.3 | 4.1 | 4.1 | 2.6 | 0 | 0 | 0 |
Nuclear Periphery | -1.0 | -4.0 | -6.1 | -6.0 | -6.3 | -7.9 | -8.7 | -8.2 | -6.8 | -4.2 | -4.2 | -6.3 | -4.5 | -6.3 | -2.7 |
Nucleolus | 3.9 | -0.1 | 3.5 | 4.1 | 5.9 | 5.8 | 6.3 | 6.5 | 8.2 | -5.0 | -5.1 | -6.6 | -2.9 | -0.9 | -1.3 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3.9 | 0 | 0 | 0 |
Vacuole | -2.5 | -0.4 | -3.2 | -5.3 | -5.9 | -4.4 | -5.6 | -6.9 | -5.8 | 2.7 | 2.7 | 5.1 | 5.0 | 4.1 | 4.5 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 7.5217 | 7.4632 | 6.7619 | 6.3754 | 7.2331 | 6.7118 | 9.1812 | 9.4465 | 8.8883 | 8.6795 | 8.7619 | 8.8441 | 10.2631 | 9.5296 | 9.2023 | 9.57 | 7.7167 | 9.1844 |
Actin | 0.0084 | 0 | 0.0102 | 0.0004 | 0.0008 | 0.0039 | 0.008 | 0.0001 | 0.0203 | 0.0114 | 0.0021 | 0.0018 | 0.0001 | 0.0002 | 0.0004 | 0.0006 | 0.0001 | 0 |
Bud | 0.0001 | 0 | 0.0001 | 0 | 0 | 0.0001 | 0.0002 | 0 | 0.0001 | 0.0001 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0.0005 | 0 | 0.0007 | 0 | 0 | 0.0001 | 0.0002 | 0 | 0.0005 | 0.0002 | 0.0001 | 0 | 0 | 0 | 0.0002 | 0.0001 | 0 | 0 |
Bud Periphery | 0.0002 | 0 | 0.0001 | 0.0001 | 0.0001 | 0.0002 | 0.0004 | 0.0001 | 0.0002 | 0.0004 | 0.0002 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0002 | 0 | 0 |
Bud Site | 0.0007 | 0 | 0.0046 | 0 | 0 | 0.0002 | 0.0006 | 0 | 0.0007 | 0.0012 | 0 | 0 | 0 | 0 | 0.0009 | 0 | 0 | 0 |
Cell Periphery | 0.0001 | 0 | 0.0001 | 0 | 0.0001 | 0 | 0.0001 | 0 | 0.0001 | 0.0001 | 0 | 0 | 0 | 0 | 0.0001 | 0 | 0 | 0 |
Cytoplasm | 0.002 | 0 | 0.0005 | 0.0002 | 0 | 0.0002 | 0.0039 | 0 | 0.0009 | 0.0004 | 0.0067 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasmic Foci | 0.0049 | 0 | 0.004 | 0.0007 | 0.0001 | 0.0012 | 0.0074 | 0 | 0.0026 | 0.0079 | 0.0013 | 0.0007 | 0 | 0 | 0.0013 | 0 | 0 | 0.0001 |
Eisosomes | 0.0001 | 0 | 0.0001 | 0 | 0.0001 | 0 | 0.0001 | 0 | 0.0001 | 0.0002 | 0.0002 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endoplasmic Reticulum | 0.0024 | 0.0003 | 0.0008 | 0.0016 | 0.0013 | 0.0004 | 0.0042 | 0.0006 | 0.0016 | 0.0012 | 0.0014 | 0.0002 | 0.0003 | 0.0007 | 0.0004 | 0.0015 | 0.0003 | 0.0001 |
Endosome | 0.0071 | 0.0001 | 0.0021 | 0.0014 | 0.0014 | 0.003 | 0.0131 | 0.0003 | 0.0053 | 0.0041 | 0.007 | 0.001 | 0.0001 | 0.0001 | 0.0024 | 0.0005 | 0.0003 | 0.0001 |
Golgi | 0.0022 | 0 | 0.0011 | 0.0004 | 0.0035 | 0.0009 | 0.0038 | 0.0001 | 0.0021 | 0.0039 | 0.0008 | 0.001 | 0.0001 | 0 | 0.0012 | 0.0002 | 0.0002 | 0.0002 |
Lipid Particles | 0.0061 | 0.0001 | 0.0076 | 0.0076 | 0.0045 | 0.0042 | 0.0135 | 0.002 | 0.0155 | 0.0066 | 0.0025 | 0.0027 | 0.0005 | 0.0002 | 0.0063 | 0.0007 | 0.0031 | 0.0097 |
Mitochondria | 0.0014 | 0.0002 | 0.0009 | 0.001 | 0.0053 | 0.0013 | 0.0044 | 0.0132 | 0.0022 | 0.0181 | 0.0167 | 0.0005 | 0.0007 | 0.0012 | 0.0014 | 0.0019 | 0.0003 | 0.0002 |
None | 0.0022 | 0 | 0.0005 | 0.0003 | 0.0002 | 0.0004 | 0.0017 | 0.0001 | 0.0008 | 0.0008 | 0.0077 | 0.0001 | 0.0001 | 0.0001 | 0.0002 | 0.0002 | 0.0001 | 0 |
Nuclear Periphery | 0.91 | 0.9519 | 0.9108 | 0.9421 | 0.9449 | 0.9459 | 0.8876 | 0.928 | 0.8994 | 0.8818 | 0.8917 | 0.9719 | 0.9697 | 0.9318 | 0.944 | 0.9509 | 0.9667 | 0.9726 |
Nucleolus | 0.0015 | 0.0006 | 0.0036 | 0.0004 | 0.0005 | 0.0013 | 0.0006 | 0.0003 | 0.0009 | 0.0006 | 0.0009 | 0.0003 | 0.0001 | 0.0005 | 0.0007 | 0.0003 | 0.0003 | 0.0002 |
Nucleus | 0.012 | 0.0259 | 0.0123 | 0.0109 | 0.0036 | 0.0188 | 0.0063 | 0.0038 | 0.0083 | 0.0079 | 0.0083 | 0.0024 | 0.0009 | 0.0227 | 0.0029 | 0.0029 | 0.0032 | 0.0014 |
Peroxisomes | 0.003 | 0 | 0.0034 | 0.0001 | 0 | 0.0007 | 0.009 | 0 | 0.0013 | 0.0103 | 0.0002 | 0.0017 | 0 | 0 | 0.0007 | 0 | 0 | 0 |
Punctate Nuclear | 0.0159 | 0.0002 | 0.0086 | 0.0014 | 0.0005 | 0.0022 | 0.0036 | 0.0003 | 0.0031 | 0.0023 | 0.0153 | 0.0009 | 0.0002 | 0.0001 | 0.0006 | 0.0005 | 0.0007 | 0.0003 |
Vacuole | 0.001 | 0.0002 | 0.0004 | 0.0008 | 0.0006 | 0.0017 | 0.0042 | 0.0007 | 0.0015 | 0.0006 | 0.0006 | 0.0001 | 0.0001 | 0.0004 | 0.0006 | 0.0005 | 0.0002 | 0.0001 |
Vacuole Periphery | 0.0181 | 0.0204 | 0.0277 | 0.0305 | 0.0325 | 0.0131 | 0.0269 | 0.0503 | 0.0325 | 0.0399 | 0.0363 | 0.0145 | 0.027 | 0.0417 | 0.0355 | 0.039 | 0.0243 | 0.015 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 13.5221 | 26.2481 | 18.4521 | 20.097 | 20.5375 | 8.4949 | 21.476 | 19.5621 | 19.5614 | 23.6211 |
Translational Efficiency | 3.1437 | 2.1526 | 2.0273 | 1.5186 | 2.2884 | 4.4509 | 1.9371 | 1.8566 | 1.7681 | 1.7386 |
Dataset | Hit |
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Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✔ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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