ORF
Human Ortholog
Description Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the membrane of the vacuole; mutant has enhanced sensitivity to overexpression of mutant huntingtin; YMR160W is not an essential gene; relative distribution within the vacuolar membrane changes upon DNA replication stress
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 – 0 0.06 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0.09 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.28 0.56 0.73 0.93 0.81 0.83 0.89 – 0.76 0.8 0.81 0.82 0.42 0.53 0.55 0.32 0.28 0.31 0 0.07 0.1 0 0 0
Endoplasmic Reticulum 0 0.06 0.13 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0.07 0 0 0 0 0 0 – 0 0 0 0 0 0.05 0.06 0 0 0.1 0.1 0.08 0.05 0.1 0.12 0.13
Golgi 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.11 0.08 0 0.09 0 0.09 0.14 – 0.38 0.27 0.44 0.35 0.1 0 0 0.06 0.05 0.05 0 0 0.13 0 0 0
Nucleus 0.07 0 0.05 0 0 0 0 – 0 0 0 0 0 0 0 0.07 0.09 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.59 0.38 0.08 0 0.17 0.08 0 – 0.05 0.05 0 0 0.56 0.53 0.43 0.66 0.67 0.68 0.72 0.75 0.61 0.75 0.73 0.69
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 2 0 2 0 0 1 0 – 0 0 0 1 0 0 0 0 0 0 2 1 0 0 1 1
Bud 1 1 0 0 0 3 2 – 1 9 6 4 2 0 1 0 1 0 4 5 2 3 3 6
Bud Neck 0 0 0 0 1 1 1 – 0 0 0 0 0 0 0 0 0 0 1 0 0 1 1 1
Bud Site 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 2 1 2 4 1 1 2 – 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0
Cytoplasm 106 151 58 43 78 129 182 – 97 114 112 111 118 175 211 97 107 97 8 21 14 13 12 13
Endoplasmic Reticulum 10 15 10 0 0 5 2 – 0 0 2 2 4 0 7 10 14 14 3 2 1 3 7 6
Endosome 26 9 0 0 3 3 2 – 1 0 0 0 6 16 24 10 15 31 46 24 7 33 42 45
Golgi 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 1 2 4 0 1 1 2
Mitochondria 42 22 2 4 1 14 28 – 48 39 61 48 28 12 15 17 21 16 16 6 18 7 7 9
Nucleus 25 7 4 1 0 1 1 – 5 6 1 1 3 2 8 21 35 14 5 4 1 0 1 1
Nuclear Periphery 6 1 0 0 0 0 0 – 0 2 0 0 2 3 0 4 7 1 21 3 3 8 12 9
Nucleolus 0 2 0 0 0 0 0 – 0 0 0 1 0 0 1 0 1 1 1 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 1 1 0
SpindlePole 2 1 0 0 1 1 4 – 0 0 0 2 0 0 0 1 1 3 2 3 2 0 2 2
Vac/Vac Membrane 220 103 6 0 16 12 9 – 6 7 5 4 155 175 167 201 260 215 317 236 87 246 266 236
Unique Cell Count 374 270 79 46 96 156 205 128 143 139 136 279 331 384 303 388 315 440 316 144 327 365 343
Labelled Cell Count 442 313 84 52 101 171 233 158 177 187 174 318 383 434 361 462 394 440 316 144 327 365 343


Vacuole Periphery

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.8 5.1 4.7 4.5 5.4 4.2 4.6 – 4.0 4.1 3.8 4.0 4.5 4.7 4.8 6.6 6.8 7.1 4.3 4.6 5.0
Std Deviation (1e-4) 0.7 1.1 0.7 0.8 1.2 0.9 1.4 – 1.3 1.7 1.1 0.9 0.9 1.1 1.3 1.5 1.3 1.5 1.1 1.4 1.7
Intensity Change (Log2) – – – -0.07 0.2 -0.18 -0.05 – -0.25 -0.21 -0.34 -0.23 -0.07 -0.02 0.01 0.48 0.52 0.58 -0.13 -0.05 0.08


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Cytoplasm 2.7 1.2 1.7 3.2 – 0.4 1.1 1.2 1.4 -4.9 -3.3 -3.0 -6.7 -7.8 -6.9
Endoplasmic Reticulum 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 – 0 0 0 0 0 0 0 0 0 2.9
Golgi 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 – 0 0 0 0 0 0 0 0.6 1.2 0
Nuclear Periphery 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Vacuole 0 1.8 0 0 – 0 0 0 0 7.6 7.3 6.0 9.3 9.7 9.7

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 2.3307 2.8921 2.4626 2.2634 2.4876 2.5691 0.2875 0.3715 0.2557 0.0546 0.6387 0.252 0.1157 0.3389 0.047 0.326 0.5113 0.3648
Actin 0.0409 0.0004 0.0105 0.0024 0.0005 0.0016 0.0201 0.0001 0.0104 0.0017 0.0083 0.0011 0.0223 0.004 0.0062 0.0001 0.0149 0.0083
Bud 0.0016 0.0023 0.0013 0.0029 0.0007 0.0007 0.0032 0.0049 0.0013 0.005 0.0102 0.002 0.0024 0.0014 0.0057 0.0003 0.0004 0.0003
Bud Neck 0.0126 0.0018 0.0035 0.0038 0.0006 0.0031 0.002 0.0007 0.0055 0.001 0.0018 0.0017 0.0019 0.0028 0.0008 0.0002 0.0005 0.001
Bud Periphery 0.0026 0.003 0.0027 0.0057 0.0023 0.0019 0.0037 0.001 0.0016 0.0141 0.0227 0.0011 0.0032 0.0056 0.0021 0.0003 0.0005 0.0004
Bud Site 0.013 0.0121 0.0027 0.0064 0.0009 0.0008 0.007 0.0185 0.006 0.0025 0.0088 0.0013 0.0079 0.0017 0.0055 0.0002 0.0008 0.0003
Cell Periphery 0.0012 0.0009 0.0007 0.0007 0.001 0.0009 0.0008 0.0007 0.0009 0.0006 0.001 0.0002 0.0006 0.0007 0.0005 0.0001 0.0001 0.0001
Cytoplasm 0.0747 0.1459 0.1189 0.0502 0.101 0.1572 0.1153 0.263 0.1651 0.0517 0.1288 0.1233 0.0954 0.1501 0.1382 0.1024 0.1401 0.1373
Cytoplasmic Foci 0.0561 0.0327 0.0503 0.0566 0.0862 0.0528 0.0585 0.0379 0.0584 0.0329 0.0794 0.0542 0.0522 0.0261 0.0444 0.0369 0.0331 0.0583
Eisosomes 0.0006 0.0004 0.0003 0.0001 0.0001 0.0001 0.0005 0 0.0004 0 0.0001 0 0.0003 0.0001 0 0 0.0004 0
Endoplasmic Reticulum 0.0081 0.007 0.0077 0.0048 0.004 0.0063 0.0095 0.0049 0.0057 0.0058 0.003 0.0042 0.0089 0.006 0.0057 0.002 0.0048 0.0055
Endosome 0.2177 0.1767 0.242 0.2345 0.2637 0.2683 0.3061 0.2613 0.289 0.367 0.3914 0.3495 0.2438 0.2075 0.2737 0.3249 0.2895 0.3376
Golgi 0.0124 0.0031 0.0086 0.0063 0.0066 0.0048 0.0186 0.0023 0.0083 0.0113 0.0393 0.0049 0.0134 0.0013 0.0036 0.0015 0.0241 0.0044
Lipid Particles 0.0234 0.0039 0.0094 0.0111 0.0095 0.0058 0.0252 0.002 0.0088 0.0049 0.0119 0.0034 0.0213 0.0018 0.0045 0.002 0.0254 0.0097
Mitochondria 0.0192 0.0094 0.0166 0.0172 0.0392 0.0126 0.0291 0.0016 0.013 0.0082 0.0142 0.0055 0.0117 0.0017 0.0073 0.0018 0.012 0.0035
None 0.2242 0.3349 0.2714 0.142 0.1212 0.199 0.0684 0.1072 0.1085 0.058 0.0179 0.0593 0.1505 0.1287 0.1054 0.0886 0.1182 0.1172
Nuclear Periphery 0.0121 0.0068 0.0157 0.007 0.0335 0.0072 0.0202 0.0043 0.0069 0.0101 0.0034 0.0134 0.0125 0.0074 0.014 0.0039 0.0088 0.0068
Nucleolus 0.0026 0.0013 0.0024 0.0039 0.0011 0.0017 0.0048 0.0016 0.0023 0.0023 0.0006 0.0016 0.003 0.0011 0.0021 0.0005 0.0004 0.0011
Nucleus 0.0064 0.0043 0.0096 0.0104 0.0055 0.0108 0.0162 0.0106 0.0075 0.0129 0.0028 0.0091 0.011 0.0049 0.017 0.0023 0.0027 0.0082
Peroxisomes 0.0121 0.0005 0.0073 0.0064 0.0162 0.0021 0.013 0.0003 0.0044 0.0013 0.0033 0.0008 0.0115 0.0003 0.0029 0.0005 0.0086 0.0023
Punctate Nuclear 0.0037 0.001 0.002 0.0158 0.001 0.0013 0.0036 0.0016 0.0117 0.0027 0.0009 0.0031 0.0036 0.0009 0.0034 0.0003 0.0013 0.0061
Vacuole 0.2279 0.2085 0.1789 0.3431 0.2381 0.2102 0.2367 0.2288 0.225 0.3135 0.1917 0.2925 0.2852 0.3791 0.2932 0.3581 0.2306 0.2473
Vacuole Periphery 0.0268 0.0433 0.0375 0.0687 0.0671 0.0507 0.0376 0.0468 0.0595 0.0926 0.0586 0.0676 0.0374 0.0668 0.0638 0.0732 0.0827 0.0441

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 4.2768 3.2195 3.2849 7.8261 6.7124 4.0494 8.0364 9.4749 7.9563 8.9274
Translational Efficiency 1.0199 1.0388 1.0082 0.5338 0.5351 1.1513 0.5639 0.6316 0.6196 0.6175

Hit Data

Dataset Hit
Protein Concentration ✔
Protein Localization ✔
Gene Expression ✘
Translational Efficiency ✘
ORF
Human Ortholog
Description Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the membrane of the vacuole; mutant has enhanced sensitivity to overexpression of mutant huntingtin; YMR160W is not an essential gene; relative distribution within the vacuolar membrane changes upon DNA replication stress
Localization
Cell Percentages vacuole (13%), cytoplasm (7%), mixed (44%)
Cell Cycle Regulation No
Subcompartmental Group N/A

YMR160W

YMR160W


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Ymr160w-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available