Standard name | |
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Human Ortholog | |
Description | Co-chaperone for Hsp40p; anchored in the ER membrane; with its homolog Ydj1p promotes ER-associated protein degradation (ERAD) of integral membrane substrates; similar to E. coli DnaJ |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 3 | 0 | 0 | – | 0 | 1 | 1 | 0 | 0 | 2 | 0 | 2 | 0 | 2 | 2 | 3 | 1 | 6 | 8 | 13 |
Bud | 0 | 0 | 1 | 0 | 0 | 8 | 4 | – | 6 | 2 | 8 | 8 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 2 | 4 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 1 | – | 4 | 3 | 4 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 1 | 0 | 3 | 1 | 2 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 3 | 3 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 1 | 3 | 8 | 1 | 5 | – | 2 | 5 | 5 | 8 | 3 | 15 | 18 | 14 | 5 | 6 | 0 | 1 | 1 | 1 | 3 | 4 |
Cytoplasm | 46 | 18 | 26 | 104 | 182 | 155 | 348 | – | 310 | 314 | 230 | 310 | 57 | 201 | 219 | 101 | 47 | 58 | 26 | 37 | 14 | 23 | 56 | 47 |
Endoplasmic Reticulum | 19 | 3 | 4 | 17 | 18 | 9 | 5 | – | 2 | 4 | 3 | 7 | 42 | 40 | 64 | 7 | 7 | 2 | 58 | 21 | 22 | 68 | 80 | 91 |
Endosome | 1 | 2 | 0 | 2 | 8 | 0 | 6 | – | 0 | 1 | 3 | 2 | 1 | 9 | 6 | 3 | 3 | 0 | 1 | 0 | 1 | 3 | 5 | 9 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 1 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 2 | 0 | 0 | 0 | 0 | 1 | 3 |
Mitochondria | 0 | 1 | 4 | 9 | 4 | 60 | 56 | – | 108 | 97 | 132 | 137 | 0 | 2 | 0 | 6 | 6 | 4 | 5 | 5 | 10 | 11 | 17 | 25 |
Nucleus | 1 | 0 | 0 | 2 | 1 | 2 | 4 | – | 7 | 3 | 3 | 6 | 0 | 0 | 1 | 0 | 2 | 3 | 0 | 1 | 0 | 1 | 0 | 0 |
Nuclear Periphery | 0 | 1 | 0 | 0 | 0 | 0 | 1 | – | 2 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 1 | 1 | – | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 1 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 3 | – | 5 | 6 | 3 | 4 | 0 | 2 | 0 | 4 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 |
Vac/Vac Membrane | 2 | 2 | 1 | 12 | 23 | 7 | 41 | – | 19 | 16 | 14 | 20 | 2 | 9 | 1 | 45 | 19 | 20 | 3 | 6 | 0 | 3 | 6 | 7 |
Unique Cell Count | 66 | 26 | 32 | 134 | 232 | 208 | 431 | 414 | 410 | 351 | 444 | 98 | 260 | 284 | 152 | 70 | 81 | 105 | 81 | 56 | 130 | 187 | 216 | |
Labelled Cell Count | 69 | 27 | 37 | 149 | 247 | 243 | 476 | 466 | 452 | 409 | 506 | 105 | 282 | 309 | 184 | 90 | 97 | 105 | 81 | 56 | 130 | 187 | 216 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 6.8 | 5.5 | 5.5 | 6.7 | 7.0 | 4.5 | 5.1 | – | 4.1 | 4.2 | 4.2 | 4.1 | 7.2 | 6.2 | 6.8 | 10.2 | 9.7 | 10.4 | 6.0 | 6.0 | 6.5 |
Std Deviation (1e-4) | 2.4 | 1.3 | 0.9 | 1.5 | 1.5 | 1.1 | 1.4 | – | 1.0 | 1.1 | 1.5 | 1.0 | 1.4 | 1.2 | 1.4 | 3.4 | 2.5 | 2.8 | 1.0 | 1.5 | 1.5 |
Intensity Change (Log2) | – | – | – | 0.29 | 0.35 | -0.26 | -0.11 | – | -0.4 | -0.39 | -0.38 | -0.4 | 0.4 | 0.19 | 0.32 | 0.91 | 0.84 | 0.93 | 0.15 | 0.13 | 0.24 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | -0.4 | -0.4 | -0.8 | -0.1 | – | -0.8 | -0.6 | -1.8 | -1.4 | -2.4 | -0.5 | -0.5 | -1.6 | -1.5 | -1.1 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 3.1 | 0 | 1.3 | 0 | 0 | 0 |
Endosome | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3.1 | 2.8 | 2.7 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 0.7148 | 1.0748 | 0.9845 | 0.3379 | 0.7355 | 1.0622 | 1.86 | 2.6584 | 2.6132 | 1.7549 | 1.4581 | 2.3927 | 2.748 | 4.6414 | 3.6824 | 3.9204 | 2.7709 | 3.683 |
Actin | 0.0162 | 0.0036 | 0.0027 | 0.0022 | 0.0159 | 0.0012 | 0.0722 | 0.0014 | 0.0047 | 0.0023 | 0.045 | 0.0013 | 0.0016 | 0.0003 | 0.0026 | 0.0019 | 0.0015 | 0.0006 |
Bud | 0.0003 | 0.0002 | 0.0001 | 0.0001 | 0.0002 | 0 | 0.0005 | 0.0004 | 0.0006 | 0.001 | 0.0007 | 0 | 0 | 0 | 0.0001 | 0.0001 | 0.0001 | 0 |
Bud Neck | 0.0006 | 0.0016 | 0.0005 | 0.0005 | 0.0014 | 0.0014 | 0.0039 | 0.0004 | 0.0008 | 0.0013 | 0.0028 | 0.0017 | 0.0002 | 0.0003 | 0.0004 | 0.0015 | 0.0025 | 0.0023 |
Bud Periphery | 0.0003 | 0.0004 | 0.0001 | 0.0001 | 0.0006 | 0 | 0.0007 | 0.0002 | 0.0008 | 0.001 | 0.001 | 0.0001 | 0 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0 |
Bud Site | 0.0013 | 0.0104 | 0.0018 | 0.0003 | 0.0003 | 0.0001 | 0.0056 | 0.003 | 0.0061 | 0.0064 | 0.0074 | 0.0001 | 0.0003 | 0.0021 | 0.0008 | 0.0003 | 0.0003 | 0.0001 |
Cell Periphery | 0.0008 | 0.0012 | 0.001 | 0.0005 | 0.0005 | 0.0003 | 0.0008 | 0.0009 | 0.0012 | 0.0005 | 0.0005 | 0.0003 | 0.0011 | 0.0018 | 0.0013 | 0.0006 | 0.0005 | 0.0004 |
Cytoplasm | 0.4399 | 0.4995 | 0.5512 | 0.448 | 0.5136 | 0.5133 | 0.3022 | 0.505 | 0.5085 | 0.3685 | 0.4197 | 0.4919 | 0.3901 | 0.5483 | 0.5481 | 0.4282 | 0.6549 | 0.5938 |
Cytoplasmic Foci | 0.0441 | 0.0375 | 0.0299 | 0.0118 | 0.035 | 0.031 | 0.052 | 0.0464 | 0.0262 | 0.0481 | 0.0373 | 0.0227 | 0.0409 | 0.0339 | 0.0147 | 0.0231 | 0.0244 | 0.0109 |
Eisosomes | 0.0003 | 0.0001 | 0.0001 | 0.0001 | 0.0004 | 0.0001 | 0.0006 | 0 | 0.0001 | 0.0001 | 0.0003 | 0 | 0.0002 | 0.0001 | 0.0001 | 0 | 0 | 0 |
Endoplasmic Reticulum | 0.2173 | 0.1954 | 0.23 | 0.3627 | 0.1827 | 0.257 | 0.3379 | 0.3095 | 0.3044 | 0.3503 | 0.2411 | 0.3752 | 0.3091 | 0.2029 | 0.2642 | 0.4143 | 0.1878 | 0.2522 |
Endosome | 0.0519 | 0.0577 | 0.0442 | 0.0237 | 0.0614 | 0.0449 | 0.0601 | 0.0385 | 0.0354 | 0.0608 | 0.0591 | 0.0359 | 0.0369 | 0.0152 | 0.021 | 0.0298 | 0.0183 | 0.0148 |
Golgi | 0.011 | 0.0047 | 0.0022 | 0.001 | 0.0098 | 0.0015 | 0.014 | 0.0036 | 0.004 | 0.0115 | 0.0308 | 0.0047 | 0.0038 | 0.001 | 0.0017 | 0.0019 | 0.0031 | 0.0006 |
Lipid Particles | 0.0393 | 0.0207 | 0.0134 | 0.0055 | 0.0111 | 0.007 | 0.0288 | 0.0206 | 0.0174 | 0.012 | 0.0712 | 0.0149 | 0.0411 | 0.0206 | 0.0143 | 0.0115 | 0.0146 | 0.0026 |
Mitochondria | 0.0037 | 0.0053 | 0.0006 | 0.0005 | 0.0024 | 0.0005 | 0.0039 | 0.0003 | 0.0159 | 0.0111 | 0.0406 | 0.0024 | 0.0005 | 0.0001 | 0.0005 | 0.0004 | 0.0003 | 0.0002 |
None | 0.1147 | 0.1336 | 0.0866 | 0.1175 | 0.1256 | 0.1073 | 0.0619 | 0.0506 | 0.0525 | 0.0974 | 0.0161 | 0.0315 | 0.1492 | 0.1608 | 0.1147 | 0.0575 | 0.0834 | 0.1123 |
Nuclear Periphery | 0.0159 | 0.012 | 0.0123 | 0.012 | 0.0216 | 0.0134 | 0.0134 | 0.0083 | 0.0074 | 0.0113 | 0.0075 | 0.0071 | 0.0151 | 0.0044 | 0.0064 | 0.013 | 0.0031 | 0.0034 |
Nucleolus | 0.0209 | 0.0004 | 0.0071 | 0.0001 | 0.0007 | 0.0076 | 0.0037 | 0.0001 | 0.0002 | 0.0008 | 0.0004 | 0.0001 | 0.0001 | 0.0001 | 0.0004 | 0.0003 | 0 | 0.0001 |
Nucleus | 0.0057 | 0.0039 | 0.0058 | 0.0039 | 0.004 | 0.0047 | 0.0045 | 0.0024 | 0.0019 | 0.0026 | 0.0022 | 0.0025 | 0.0012 | 0.0021 | 0.0031 | 0.005 | 0.0014 | 0.0021 |
Peroxisomes | 0.0075 | 0.0006 | 0.0003 | 0.0002 | 0.0006 | 0.0004 | 0.0115 | 0.0005 | 0.0059 | 0.0012 | 0.0076 | 0.0025 | 0.0015 | 0.0001 | 0.0003 | 0.0002 | 0.0004 | 0.0001 |
Punctate Nuclear | 0.0022 | 0.0034 | 0.0027 | 0.0016 | 0.0045 | 0.0023 | 0.0155 | 0.002 | 0.0013 | 0.0046 | 0.0019 | 0.0007 | 0.0025 | 0.0014 | 0.0016 | 0.0053 | 0.0008 | 0.0009 |
Vacuole | 0.0052 | 0.007 | 0.0069 | 0.007 | 0.0059 | 0.0053 | 0.0048 | 0.0057 | 0.0043 | 0.0065 | 0.0041 | 0.0039 | 0.0041 | 0.0043 | 0.0034 | 0.0045 | 0.0022 | 0.0025 |
Vacuole Periphery | 0.0009 | 0.0008 | 0.0006 | 0.0006 | 0.0017 | 0.0006 | 0.0013 | 0.0003 | 0.0005 | 0.0011 | 0.0026 | 0.0006 | 0.0007 | 0.0001 | 0.0003 | 0.0005 | 0.0002 | 0.0002 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 45.7709 | 40.9163 | 34.6991 | 38.1104 | 37.0899 | 42.478 | 43.5867 | 41.3848 | 33.0545 | 42.4977 |
Translational Efficiency | 1.9521 | 1.7037 | 1.7977 | 1.4107 | 2.04 | 2.1524 | 1.5555 | 1.9868 | 2.153 | 1.7839 |
Dataset | Hit |
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Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✔ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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