Standard name
Human Ortholog
Description Trans-golgi network aminophospholipid translocase (flippase); type 4 P-type ATPase; involved in phospholipid translocation, contributing to the maintenance of membrane lipid asymmetry in post-Golgi secretory vesicles; role in protein trafficking between the Golgi and endosomal system; localizes to the trans-Golgi network; localizes to the shmoo tip where it has a redundant role in the cellular response to mating pheromone
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0.21 0.23 0.05 0 0 0 0 0 – 0 0 0 0.09 0.07 0 0 0.06 0 0.16 0.12 0.17 0.21 0.19 0.17
Bud 0 0.06 0.1 0 0 0.07 0.07 0.08 – 0.06 0.11 0.11 0 0 0 0 0 0.06 0 0.06 0 0.14 0.1 0.07
Bud Neck 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0.07 0 0.08 0 0.05
Bud Site 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.47 0.44 0.44 0.68 0.73 0.68 0.63 0.59 – 0.57 0.58 0.5 0.7 0.6 0.59 0.85 0.79 0.83 0.08 0.09 0.05 0.07 0.09 0.11
Endoplasmic Reticulum 0.15 0.19 0 0 0 0 0 0 – 0 0 0 0.09 0.16 0 0 0 0.09 0.05 0 0 0.06 0.06 0.08
Endosome 0.11 0 0.2 0.13 0.13 0 0 0.05 – 0 0 0 0.08 0.14 0.23 0 0.06 0 0.21 0.13 0.22 0.07 0.06 0.06
Golgi 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0.07 0 0.07 0 0 0
Mitochondria 0.07 0.22 0.11 0.14 0.09 0.41 0.39 0.4 – 0.52 0.47 0.56 0 0 0 0.07 0 0 0.22 0.27 0.26 0.22 0.26 0.23
Nucleus 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0.07 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0.07 0 0.14 0 0 0 0 0 – 0 0 0 0 0 0.08 0 0.05 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0.05 0 0 0 – 0 0 0 0 0 0.07 0 0 0 0.09 0.13 0.07 0.08 0.13 0.15
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 91 74 24 3 0 2 0 4 – 1 1 0 20 20 7 1 5 1 120 54 122 79 112 87
Bud 13 19 48 10 15 40 44 55 – 33 57 72 5 5 2 0 1 2 28 26 23 54 57 36
Bud Neck 1 2 4 1 0 0 0 1 – 1 0 1 0 0 0 2 0 0 26 32 23 28 24 23
Bud Site 7 6 11 4 4 10 7 5 – 16 10 6 0 0 0 0 1 1 – – – – – –
Cell Periphery 4 0 5 1 0 1 2 0 – 2 1 2 2 4 1 2 3 0 1 1 2 2 8 7
Cytoplasm 206 143 219 206 295 408 400 398 – 331 314 330 149 161 134 52 63 29 59 40 36 26 50 55
Endoplasmic Reticulum 68 63 11 4 3 9 4 3 – 9 1 2 19 42 8 0 3 3 38 13 31 22 37 42
Endosome 47 1 98 39 53 7 15 32 – 3 2 10 17 38 52 1 5 0 151 58 161 25 38 32
Golgi 2 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 50 18 50 10 13 11
Mitochondria 33 71 54 41 37 250 246 269 – 307 252 369 2 3 0 4 3 1 162 116 188 84 153 115
Nucleus 4 3 2 1 6 0 3 3 – 0 5 1 0 0 0 2 3 1 1 0 0 0 0 0
Nuclear Periphery 1 1 0 0 0 1 0 1 – 0 0 1 0 0 0 0 0 0 0 0 2 1 1 2
Nucleolus 1 0 1 0 1 1 1 3 – 2 1 3 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 1 0 35 7 12 3 4 8 – 0 0 5 4 5 8 0 0 0 10 4 16 5 2 3
SpindlePole 29 3 71 10 11 10 24 28 – 12 13 16 9 11 18 2 4 1 8 5 9 3 3 4
Vac/Vac Membrane 15 3 22 9 19 10 16 10 – 9 7 12 2 9 15 1 1 0 67 58 48 31 76 73
Unique Cell Count 442 324 498 302 406 604 631 670 585 537 659 212 267 226 61 80 35 737 440 730 379 591 502
Labelled Cell Count 523 389 605 336 456 752 766 820 726 664 830 229 298 245 67 92 39 737 440 730 379 591 502


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.9 5.1 5.0 4.7 4.3 3.5 3.5 3.9 – 3.1 3.3 3.4 6.4 6.4 6.9 5.1 5.3 5.7 6.4 6.4 6.5
Std Deviation (1e-4) 0.7 0.7 1.1 1.5 1.6 0.9 1.0 0.9 – 0.8 0.9 0.8 0.8 0.8 0.9 1.3 1.0 1.6 1.3 1.3 1.4
Intensity Change (Log2) – – – -0.09 -0.2 -0.52 -0.52 -0.36 – -0.66 -0.58 -0.56 0.37 0.36 0.46 0.04 0.1 0.2 0.36 0.36 0.4


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 – 0 0 0 2.3 1.5 -1.1 0 0 0
Bud 0 0 0 0 0 – 0 0 0 -3.4 -4.0 -4.3 -2.5 -2.5 0
Bud Neck 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Cytoplasm 6.7 8.7 7.9 6.5 5.2 – 4.1 4.7 2.1 6.4 4.3 3.8 6.1 5.8 4.5
Endoplasmic Reticulum 0 0 0 0 0 – 0 0 0 4.1 7.0 1.0 0 0 0
Endosome 0 0 0 0 0 – 0 0 0 -3.9 -1.9 1.0 -3.5 -2.9 -2.9
Golgi 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 – 0 0 0 -4.5 -4.9 -5.1 -1.0 -2.0 0
Nucleus 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 – 0 0 0 -2.8 -3.1 -1.8 0 0 0
SpindlePole 0 0 0 0 0 – 0 0 0 -3.9 -4.3 -2.4 -2.4 -2.3 0
Vacuole -1.0 0.2 -2.7 -1.7 -3.0 – -2.8 -3.0 -2.6 -2.3 -0.7 1.3 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 2.0255 2.078 1.6209 2.0007 1.9182 1.7129 4.5921 3.8028 3.6271 3.2621 4.5612 4.0661 3.5083 3.825 3.6478 4.1425 3.9778 3.9258
Actin 0.0573 0.0212 0.0145 0.0389 0.0098 0.0226 0.0503 0.0089 0.0113 0.0008 0.0554 0.0148 0.0257 0.0084 0.0095 0.011 0.0631 0.0315
Bud 0.0069 0.0082 0.0045 0.0022 0.0263 0.003 0.003 0.0085 0.0077 0.0017 0.0036 0.0117 0.0111 0.0115 0.0055 0.0071 0.0026 0.0031
Bud Neck 0.0222 0.0035 0.0011 0.0037 0.0016 0.0922 0.0178 0.0024 0.0044 0.0003 0.0031 0.1062 0.0346 0.003 0.0007 0.004 0.0554 0.1592
Bud Periphery 0.0055 0.0086 0.0053 0.0054 0.0087 0.0055 0.0058 0.0078 0.0107 0.0026 0.003 0.0112 0.0175 0.007 0.0076 0.0078 0.0027 0.0033
Bud Site 0.0625 0.1328 0.0258 0.0045 0.0077 0.0027 0.0407 0.1051 0.0341 0.0005 0.0035 0.0018 0.0621 0.1238 0.0105 0.0115 0.0229 0.0068
Cell Periphery 0.0014 0.0022 0.0015 0.0009 0.0003 0.0008 0.0021 0.0016 0.0012 0.0001 0.0002 0.0018 0.003 0.002 0.0005 0.0007 0.0004 0.0009
Cytoplasm 0.1899 0.2876 0.3081 0.2338 0.2381 0.3058 0.2325 0.3128 0.2622 0.2644 0.0947 0.3477 0.2786 0.3628 0.3701 0.2683 0.2154 0.3853
Cytoplasmic Foci 0.1376 0.0929 0.1258 0.1247 0.1036 0.1503 0.1222 0.136 0.1061 0.1069 0.1277 0.0829 0.1105 0.087 0.1146 0.1323 0.1105 0.0876
Eisosomes 0.0007 0.0006 0.0004 0.0004 0.0001 0.0002 0.0009 0.0003 0.0003 0.0001 0.0003 0.0002 0.0005 0.0002 0.0002 0.0001 0.0001 0.0002
Endoplasmic Reticulum 0.0126 0.0079 0.009 0.0174 0.0054 0.0135 0.0142 0.0068 0.0089 0.0088 0.0056 0.0055 0.0186 0.0072 0.0055 0.0097 0.0095 0.0043
Endosome 0.1428 0.108 0.1937 0.3009 0.2283 0.1238 0.1094 0.1025 0.1157 0.3326 0.178 0.0692 0.1861 0.1258 0.2182 0.1981 0.2345 0.0836
Golgi 0.0251 0.0214 0.0284 0.0202 0.0248 0.0338 0.0195 0.0157 0.012 0.0127 0.0243 0.0097 0.0199 0.0241 0.0173 0.0308 0.0384 0.0166
Lipid Particles 0.02 0.0043 0.0051 0.0085 0.0112 0.0117 0.016 0.0024 0.0038 0.0038 0.0264 0.0032 0.0172 0.0034 0.0061 0.0054 0.0076 0.0021
Mitochondria 0.017 0.0144 0.0102 0.0075 0.0089 0.0103 0.0096 0.0102 0.0049 0.0049 0.0048 0.0081 0.0064 0.0076 0.0146 0.019 0.0062 0.0039
None 0.2331 0.2544 0.2375 0.1824 0.2602 0.1934 0.2676 0.2521 0.385 0.2218 0.3883 0.3002 0.1535 0.2034 0.1744 0.2377 0.1952 0.1996
Nuclear Periphery 0.0137 0.0018 0.0032 0.0077 0.0109 0.0016 0.026 0.0021 0.0021 0.0043 0.007 0.0054 0.0163 0.0019 0.0018 0.0021 0.001 0.0006
Nucleolus 0.0019 0.0009 0.0007 0.0005 0.0007 0.0006 0.0017 0.0007 0.0013 0.0002 0.0005 0.0008 0.0007 0.0005 0.0004 0.0018 0.0003 0.0001
Nucleus 0.0118 0.0025 0.0041 0.005 0.0039 0.0019 0.0167 0.0024 0.0035 0.0015 0.0017 0.0074 0.0033 0.0021 0.0017 0.002 0.0006 0.0007
Peroxisomes 0.0161 0.0066 0.0068 0.0069 0.024 0.0168 0.0135 0.0067 0.0082 0.0014 0.0513 0.0021 0.0119 0.0038 0.0217 0.0307 0.0249 0.0048
Punctate Nuclear 0.0117 0.0028 0.0033 0.0107 0.0081 0.0018 0.0117 0.0026 0.0048 0.0013 0.0129 0.0023 0.0016 0.0016 0.0039 0.0047 0.0016 0.0008
Vacuole 0.008 0.0156 0.0088 0.0134 0.0142 0.0062 0.0151 0.0107 0.01 0.0225 0.0055 0.0062 0.0176 0.0114 0.0121 0.0122 0.0048 0.0041
Vacuole Periphery 0.0022 0.0019 0.0025 0.0046 0.0031 0.0014 0.0038 0.0017 0.0018 0.0068 0.0022 0.0016 0.0034 0.0016 0.003 0.0029 0.0022 0.0009

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 13.2071 11.7071 11.3375 18.7853 13.814 12.2015 16.5017 17.8065 18.307 21.3597
Translational Efficiency 0.4252 0.335 0.4578 0.2852 0.3606 0.4212 0.2949 0.2538 0.3045 0.2606

Hit Data

Dataset Hit
Protein Concentration ✔
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Trans-golgi network aminophospholipid translocase (flippase); type 4 P-type ATPase; involved in phospholipid translocation, contributing to the maintenance of membrane lipid asymmetry in post-Golgi secretory vesicles; role in protein trafficking between the Golgi and endosomal system; localizes to the trans-Golgi network; localizes to the shmoo tip where it has a redundant role in the cellular response to mating pheromone
Localization
Cell Percentages —
Cell Cycle Regulation No
Subcompartmental Group N/A

Dnf3

Dnf3


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Dnf3-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available