Standard name | |
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Human Ortholog | |
Description | Peroxisome-specific receptor important for peroxisome inheritance; co-fractionates with peroxisome membranes and co-localizes with peroxisomes in vivo; physically interacts with the myosin V motor Myo2p; INP2 is not an essential gene |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 8 | 4 | 39 | 21 | 27 | 4 | 13 | 4 | 6 | 3 | 1 | 5 | 6 | 0 | 2 | 3 | 1 | 0 | 17 | 33 | 47 | 9 | 20 | 20 |
Bud | 23 | 28 | 61 | 62 | 66 | 53 | 68 | 44 | 64 | 42 | 63 | 71 | 28 | 18 | 11 | 5 | 3 | 1 | 12 | 16 | 15 | 8 | 4 | 9 |
Bud Neck | 15 | 17 | 94 | 70 | 85 | 81 | 95 | 64 | 50 | 30 | 35 | 64 | 9 | 0 | 5 | 4 | 1 | 0 | 4 | 16 | 16 | 6 | 11 | 6 |
Bud Site | 1 | 2 | 2 | 1 | 1 | 0 | 0 | 3 | 5 | 3 | 5 | 2 | 2 | 1 | 0 | 1 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 1 | 0 | 3 | 1 | 4 | 4 | 2 | 1 | 1 | 1 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 7 | 1 | 4 | 5 |
Cytoplasm | 128 | 156 | 321 | 291 | 400 | 415 | 518 | 436 | 355 | 355 | 278 | 334 | 172 | 182 | 228 | 36 | 6 | 11 | 46 | 68 | 110 | 39 | 98 | 123 |
Endoplasmic Reticulum | 0 | 5 | 1 | 2 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 1 | 0 | 1 | 2 | 0 | 0 | 2 | 2 | 3 |
Endosome | 11 | 4 | 0 | 2 | 0 | 1 | 0 | 0 | 1 | 0 | 0 | 1 | 5 | 21 | 16 | 8 | 2 | 7 | 55 | 34 | 38 | 7 | 11 | 5 |
Golgi | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 31 | 45 | 2 | 6 | 17 | 43 | 69 | 49 | 157 | 110 | 146 | 133 | 11 | 5 | 10 | 7 | 2 | 1 | 49 | 48 | 104 | 20 | 62 | 45 |
Nucleus | 0 | 0 | 0 | 1 | 4 | 1 | 5 | 4 | 2 | 1 | 2 | 5 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 1 | 1 | 0 | 1 | 0 | 0 | 2 | 0 | 1 | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 0 |
Nucleolus | 8 | 2 | 1 | 2 | 0 | 0 | 0 | 0 | 2 | 3 | 1 | 1 | 1 | 0 | 0 | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 12 | 7 | 0 | 0 | 0 | 0 | 2 | 0 | 3 | 0 | 0 | 0 | 6 | 18 | 14 | 5 | 1 | 5 | 6 | 9 | 14 | 1 | 2 | 1 |
SpindlePole | 44 | 40 | 80 | 83 | 88 | 49 | 91 | 98 | 49 | 40 | 43 | 90 | 22 | 17 | 22 | 20 | 7 | 15 | 69 | 62 | 107 | 10 | 9 | 6 |
Vac/Vac Membrane | 2 | 5 | 0 | 0 | 1 | 2 | 5 | 7 | 0 | 0 | 4 | 6 | 0 | 0 | 0 | 7 | 1 | 1 | 7 | 8 | 8 | 0 | 6 | 7 |
Unique Cell Count | 234 | 265 | 461 | 431 | 552 | 544 | 725 | 600 | 544 | 501 | 465 | 561 | 230 | 236 | 288 | 79 | 18 | 37 | 277 | 306 | 491 | 111 | 238 | 239 |
Labelled Cell Count | 285 | 316 | 605 | 543 | 693 | 654 | 870 | 710 | 696 | 589 | 580 | 713 | 264 | 262 | 309 | 100 | 24 | 43 | 277 | 306 | 491 | 111 | 238 | 239 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 4.0 | 3.6 | 4.4 | 4.0 | 4.3 | 3.4 | 3.6 | 3.9 | 3.0 | 2.9 | 3.0 | 3.6 | 3.9 | 3.9 | 4.0 | 6.0 | 5.6 | 6.3 | 3.5 | 3.5 | 4.2 |
Std Deviation (1e-4) | 0.7 | 0.5 | 0.6 | 0.9 | 0.9 | 0.8 | 0.8 | 0.9 | 0.7 | 0.6 | 0.6 | 1.0 | 1.0 | 1.0 | 0.9 | 1.4 | 0.7 | 2.1 | 1.1 | 1.0 | 1.7 |
Intensity Change (Log2) | – | – | – | -0.12 | -0.01 | -0.36 | -0.27 | -0.15 | -0.52 | -0.57 | -0.55 | -0.27 | -0.16 | -0.16 | -0.13 | 0.47 | 0.37 | 0.54 | -0.3 | -0.3 | -0.05 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Actin | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud Neck | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud Site | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cell Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cyto | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endoplasmic Reticulum | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endosome | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Golgi | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Mitochondria | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nuclear Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nuc | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleolus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Peroxisomes | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
SpindlePole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Vac | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cortical Patches | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cytoplasm | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Vacuole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | -0.6536 | -0.9017 | -0.5327 | -0.9417 | -0.089 | -0.8223 | -0.9814 | -1.0553 | -0.7768 | -0.6085 | -0.6196 | -1.0032 | -0.7669 | -1.262 | -1.1759 | -1.1148 | -0.9886 | -1.3715 |
Actin | 0.0203 | 0.0035 | 0.0327 | 0.0005 | 0.0995 | 0.006 | 0.0385 | 0.0088 | 0.0238 | 0.0034 | 0.0159 | 0.011 | 0.0536 | 0.0164 | 0.0245 | 0.001 | 0.005 | 0.0065 |
Bud | 0.0009 | 0.0018 | 0.0036 | 0.0007 | 0.0026 | 0.0005 | 0.0007 | 0.0062 | 0.007 | 0.0166 | 0.0133 | 0.0008 | 0.0018 | 0.0022 | 0.0091 | 0.0038 | 0.0011 | 0.0024 |
Bud Neck | 0.0088 | 0.0032 | 0.0267 | 0.0008 | 0.0092 | 0.0187 | 0.0024 | 0.0049 | 0.0159 | 0.0085 | 0.0009 | 0.0101 | 0.0048 | 0.0015 | 0.0264 | 0.0053 | 0.0052 | 0.0413 |
Bud Periphery | 0.003 | 0.0029 | 0.012 | 0.0024 | 0.0065 | 0.0011 | 0.0015 | 0.0126 | 0.0223 | 0.0316 | 0.0224 | 0.002 | 0.0041 | 0.0057 | 0.0275 | 0.0493 | 0.0117 | 0.0177 |
Bud Site | 0.0093 | 0.0194 | 0.0187 | 0.0009 | 0.0164 | 0.0016 | 0.0055 | 0.0339 | 0.0229 | 0.0386 | 0.0064 | 0.0007 | 0.0136 | 0.0296 | 0.0323 | 0.0079 | 0.0032 | 0.0064 |
Cell Periphery | 0.0014 | 0.0007 | 0.0007 | 0.0002 | 0.0005 | 0.0002 | 0.0005 | 0.0015 | 0.0006 | 0.0009 | 0.0002 | 0.0002 | 0.0028 | 0.0083 | 0.0152 | 0.0329 | 0.031 | 0.0179 |
Cytoplasm | 0.0415 | 0.0247 | 0.0258 | 0.0069 | 0.0058 | 0.0581 | 0.0712 | 0.118 | 0.0594 | 0.0279 | 0.0093 | 0.0919 | 0.0451 | 0.0813 | 0.0406 | 0.0171 | 0.0286 | 0.1158 |
Cytoplasmic Foci | 0.1944 | 0.1404 | 0.2821 | 0.3646 | 0.405 | 0.2303 | 0.279 | 0.1654 | 0.2703 | 0.3249 | 0.3541 | 0.2592 | 0.1563 | 0.0876 | 0.2539 | 0.3783 | 0.2149 | 0.2078 |
Eisosomes | 0.0009 | 0.0004 | 0.0013 | 0 | 0.0008 | 0.0003 | 0.0009 | 0.0006 | 0.0004 | 0.0001 | 0.0004 | 0.0002 | 0.0008 | 0.0014 | 0.0007 | 0.0002 | 0.0003 | 0.0004 |
Endoplasmic Reticulum | 0.0126 | 0.0015 | 0.0011 | 0.0003 | 0.0011 | 0.001 | 0.0022 | 0.0028 | 0.0017 | 0.0007 | 0.0009 | 0.0014 | 0.0014 | 0.0032 | 0.0013 | 0.0005 | 0.0013 | 0.0026 |
Endosome | 0.0403 | 0.0123 | 0.0144 | 0.037 | 0.0352 | 0.0169 | 0.0355 | 0.034 | 0.0179 | 0.0389 | 0.0412 | 0.0252 | 0.0096 | 0.0115 | 0.016 | 0.049 | 0.0699 | 0.0315 |
Golgi | 0.0073 | 0.0018 | 0.0051 | 0.0076 | 0.0242 | 0.004 | 0.009 | 0.0042 | 0.0107 | 0.0218 | 0.0157 | 0.0046 | 0.0062 | 0.0019 | 0.009 | 0.0074 | 0.0177 | 0.0064 |
Lipid Particles | 0.0328 | 0.0171 | 0.0184 | 0.0668 | 0.0613 | 0.0257 | 0.0288 | 0.0119 | 0.0174 | 0.0334 | 0.0803 | 0.0164 | 0.0205 | 0.0103 | 0.0104 | 0.0724 | 0.1559 | 0.0442 |
Mitochondria | 0.0048 | 0.0017 | 0.0115 | 0.0194 | 0.009 | 0.006 | 0.016 | 0.0036 | 0.0151 | 0.0095 | 0.0079 | 0.0125 | 0.0157 | 0.0033 | 0.0179 | 0.0194 | 0.0507 | 0.0072 |
None | 0.5345 | 0.7031 | 0.4032 | 0.1232 | 0.1004 | 0.5236 | 0.4104 | 0.5359 | 0.2643 | 0.1564 | 0.1118 | 0.4695 | 0.6043 | 0.7106 | 0.3283 | 0.0984 | 0.2464 | 0.4237 |
Nuclear Periphery | 0.006 | 0.0011 | 0.0019 | 0.0004 | 0.0026 | 0.007 | 0.0041 | 0.0013 | 0.0008 | 0.0011 | 0.008 | 0.0043 | 0.005 | 0.0006 | 0.0007 | 0.0002 | 0.0033 | 0.0013 |
Nucleolus | 0.0019 | 0.0029 | 0.0015 | 0.0019 | 0.002 | 0.0023 | 0.0009 | 0.001 | 0.0016 | 0.0012 | 0.0011 | 0.0016 | 0.0024 | 0.0007 | 0.0011 | 0.0016 | 0.003 | 0.0017 |
Nucleus | 0.012 | 0.0015 | 0.0012 | 0.0008 | 0.0012 | 0.0028 | 0.0021 | 0.0017 | 0.0023 | 0.0017 | 0.0016 | 0.0026 | 0.0048 | 0.0008 | 0.0017 | 0.0014 | 0.0014 | 0.0022 |
Peroxisomes | 0.0512 | 0.0391 | 0.1271 | 0.2912 | 0.1998 | 0.0638 | 0.0742 | 0.0419 | 0.2352 | 0.2671 | 0.291 | 0.0631 | 0.0308 | 0.0157 | 0.1724 | 0.2301 | 0.1268 | 0.036 |
Punctate Nuclear | 0.0116 | 0.0186 | 0.0068 | 0.0633 | 0.0146 | 0.0279 | 0.0127 | 0.0054 | 0.0076 | 0.003 | 0.0144 | 0.0208 | 0.0137 | 0.0031 | 0.0032 | 0.0026 | 0.012 | 0.0227 |
Vacuole | 0.0038 | 0.0021 | 0.0028 | 0.0091 | 0.0017 | 0.0016 | 0.0029 | 0.0037 | 0.0024 | 0.0108 | 0.002 | 0.0016 | 0.0022 | 0.0035 | 0.007 | 0.0173 | 0.008 | 0.0035 |
Vacuole Periphery | 0.0008 | 0.0003 | 0.0011 | 0.002 | 0.0006 | 0.0007 | 0.0012 | 0.0007 | 0.0005 | 0.0018 | 0.001 | 0.0005 | 0.0005 | 0.0004 | 0.001 | 0.0041 | 0.0024 | 0.0007 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 13.5451 | 10.2456 | 9.3306 | 6.8568 | 9.4564 | 11.4549 | 13.891 | 12.5536 | 8.8754 | 11.3281 |
Translational Efficiency | 0.3416 | 0.4308 | 0.2632 | 0.3384 | 0.3845 | 0.4168 | 0.4041 | 0.3752 | 0.3831 | 0.3648 |
Dataset | Hit |
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Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✔ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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