Standard name
Human Ortholog
Description Transcription factor; involved in regulation of invasive growth and starch degradation; controls the activation of FLO11 and STA2 in response to nutritional signals; forms a heterodimer with Flo8p that interacts with the Swi/Snf complex during transcriptional activation of FLO1, FLO11, and STA1
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.96 0.95 0.95 0.94 0.97 0.8 0.65 0.7 0.54 0.6 0.47 0.51 0.95 0.98 0.99 0.92 0.9 0.91 0.82 0.83 0.75 0.36 0.4 0.51
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0.06 0.45 0.57 0.45 0.7 0.53 0.7 0.62 0 0 0 0 0 0 0.05 0 0.08 0.08 0.09 0.08
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.13 0.08 0.05
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.07 0.05
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0.06 0.05 0 0.08 0.07 0 0 0.1 0.09 0.11 0.07 0.05 0 0.28 0.25 0.21
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 3 1 2 4 4
Bud 0 0 0 1 0 3 7 5 7 1 8 5 0 0 0 0 0 1 0 1 1 6 7 6
Bud Neck 0 0 0 0 0 1 0 1 0 1 1 0 0 0 1 1 0 1 1 1 2 2 5 10
Bud Site 0 0 0 0 0 0 1 0 0 0 1 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 2 0 0 0 0 1 0 1
Cytoplasm 50 195 123 116 236 228 218 190 170 191 125 170 98 396 314 143 141 163 71 189 99 97 180 238
Endoplasmic Reticulum 0 1 1 0 0 1 0 1 1 5 0 0 2 5 0 7 7 11 0 0 2 2 8 5
Endosome 0 1 0 0 0 0 3 0 1 3 2 8 1 0 0 1 2 2 0 1 2 2 4 5
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 2 1 1
Mitochondria 1 2 5 4 15 129 191 121 219 170 187 205 0 0 0 2 2 1 4 9 10 22 41 39
Nucleus 0 0 0 0 1 1 2 1 5 2 4 3 0 0 0 0 1 2 1 4 0 34 37 22
Nuclear Periphery 0 0 0 0 0 3 0 2 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0
Nucleolus 0 0 0 0 0 1 0 0 0 1 1 1 0 0 0 1 0 0 0 0 0 1 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 2 1 3 4 5 9 6 0 2 0 1 0 0 1 1 1 12 29 21
Vac/Vac Membrane 2 9 4 5 5 4 9 7 20 17 7 27 7 5 0 15 14 19 5 12 5 74 110 100
Unique Cell Count 52 206 130 124 243 286 336 271 315 321 268 333 103 406 316 156 157 180 88 228 133 269 447 467
Labelled Cell Count 53 208 133 127 257 373 432 331 427 396 346 425 108 408 316 171 169 201 88 228 133 269 447 467


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.1 4.0 3.9 4.1 3.8 2.9 2.9 3.6 3.0 3.3 3.1 3.1 4.9 5.2 5.1 5.1 5.3 5.2 5.0 5.7 5.3
Std Deviation (1e-4) 1.4 1.1 1.6 1.9 1.4 1.2 1.4 1.6 1.1 1.1 1.2 1.2 0.7 1.2 1.3 0.9 1.2 0.9 1.3 9.1 1.2
Intensity Change (Log2) – – – 0.05 -0.03 -0.42 -0.41 -0.12 -0.36 -0.23 -0.33 -0.35 0.33 0.41 0.38 0.39 0.45 0.41 0.34 0.54 0.43


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – 0 0 0
Bud Neck – – – – – – – – – – – – 0 0 0
Bud Site – – – – – – – – – – – – 0 0 0
Cell Periphery – – – – – – – – – – – – 0 0 0
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – 0 0 0
Endosome – – – – – – – – – – – – 0 0 0
Golgi – – – – – – – – – – – – 0 0 0
Mitochondria – – – – – – – – – – – – 0 0 0
Nuclear Periphery – – – – – – – – – – – – 0 0 0
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – 0 0 0
Peroxisomes – – – – – – – – – – – – 0 0 0
SpindlePole – – – – – – – – – – – – 0 0 0
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – 0 0 0
Cytoplasm – – – – – – – – – – – – 0 0 0
Nucleus – – – – – – – – – – – – 0 0 0
Vacuole – – – – – – – – – – – – 2.2 2.0 2.5

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration -0.9343 -0.8579 -0.7379 -0.8464 -0.8522 -0.9505 0.1693 0.2849 0.1558 0.2039 -0.1071 0.241 -0.5841 -0.2606 -0.5725 -1.2606 -1.0582 -0.5317
Actin 0.0232 0.0004 0.0125 0.0693 0.0409 0.0003 0.015 0.0003 0.0043 0.0016 0.0002 0.0016 0.0088 0.0016 0.0226 0.0001 0.0008 0.0001
Bud 0.0001 0.0003 0.0005 0.0057 0.0033 0 0.007 0.009 0.0045 0.0004 0.0002 0.0038 0.0002 0.0008 0.0005 0 0.0005 0.0001
Bud Neck 0.0002 0.0001 0.0009 0.0006 0.0019 0.0012 0.0028 0.0004 0.0002 0.0003 0.0004 0.0032 0.0003 0.0003 0.0002 0.0001 0.0004 0.0008
Bud Periphery 0.0001 0.0003 0.0004 0.0097 0.0031 0 0.0042 0.0055 0.0015 0.0005 0.0002 0.0023 0.0001 0.0007 0.0026 0 0.0013 0.0001
Bud Site 0.0006 0.0017 0.0071 0.0055 0.0057 0.0001 0.0135 0.0105 0.0021 0.0003 0.0003 0.0006 0.0021 0.0142 0.0005 0.0001 0.0011 0.0001
Cell Periphery 0.0001 0.0001 0.0002 0.0004 0.0002 0 0.0003 0.0002 0.0002 0.0001 0.0001 0.0001 0.0002 0.0001 0.0001 0 0.0003 0
Cytoplasm 0.267 0.2868 0.2064 0.3927 0.2134 0.3802 0.2309 0.4152 0.2686 0.2288 0.2228 0.3715 0.2746 0.3632 0.3133 0.2844 0.1877 0.2909
Cytoplasmic Foci 0.0445 0.0048 0.0124 0.005 0.0261 0.022 0.0515 0.0114 0.0078 0.0094 0.0082 0.0189 0.056 0.0161 0.0056 0.0039 0.0079 0.0025
Eisosomes 0.0003 0.0001 0.0001 0.0003 0.0001 0.0001 0.0003 0 0.0001 0 0 0 0.0001 0.0001 0.0002 0 0.0001 0
Endoplasmic Reticulum 0.0037 0.0054 0.0026 0.0053 0.005 0.0045 0.006 0.0029 0.0045 0.0114 0.001 0.0024 0.0045 0.005 0.0048 0.0037 0.0024 0.0017
Endosome 0.0179 0.0016 0.0067 0.012 0.0268 0.0041 0.0371 0.0067 0.0058 0.0228 0.0028 0.0154 0.0089 0.016 0.0046 0.002 0.0331 0.0013
Golgi 0.0154 0.0001 0.0036 0.0114 0.0494 0.0001 0.0136 0.001 0.0017 0.0018 0.0009 0.0035 0.005 0.0032 0.0003 0 0.0159 0
Lipid Particles 0.0149 0.0003 0.0014 0.0011 0.0024 0.001 0.0244 0.0008 0.0006 0.001 0.0019 0.0045 0.0159 0.0032 0.0022 0.0001 0.0143 0.0001
Mitochondria 0.0022 0.0003 0.001 0.0126 0.0087 0.0006 0.021 0.016 0.0086 0.0069 0.0623 0.0017 0.0008 0.0007 0.002 0.0003 0.0574 0.0003
None 0.5072 0.6183 0.5691 0.3834 0.5191 0.3857 0.4535 0.4132 0.5146 0.5074 0.5899 0.3411 0.5008 0.4464 0.4401 0.5368 0.5702 0.5815
Nuclear Periphery 0.0036 0.0042 0.005 0.0069 0.0072 0.0156 0.0069 0.004 0.008 0.0348 0.0024 0.0069 0.0028 0.0057 0.0192 0.0052 0.0039 0.0019
Nucleolus 0.0006 0.0004 0.0006 0.0003 0.0015 0.0006 0.0021 0.0007 0.0009 0.0016 0.001 0.0019 0.0005 0.0008 0.0006 0.0006 0.0015 0.0004
Nucleus 0.0833 0.0688 0.1528 0.0703 0.0587 0.1439 0.0641 0.0903 0.1478 0.132 0.0957 0.1643 0.0772 0.1093 0.1663 0.1573 0.0812 0.114
Peroxisomes 0.0061 0.0001 0.0084 0.0008 0.0046 0.0002 0.0183 0.0017 0.0004 0.0006 0.0035 0.0151 0.0287 0.0015 0.0019 0 0.0028 0
Punctate Nuclear 0.0072 0.0046 0.0046 0.0036 0.0185 0.0381 0.0218 0.0067 0.0157 0.0312 0.0015 0.0381 0.0113 0.0091 0.0096 0.004 0.0051 0.0031
Vacuole 0.0013 0.0011 0.0034 0.002 0.0025 0.0014 0.0042 0.0029 0.0018 0.0036 0.0025 0.0022 0.0012 0.0016 0.0021 0.0011 0.0074 0.0011
Vacuole Periphery 0.0003 0.0001 0.0004 0.0012 0.0009 0.0004 0.0017 0.0005 0.0005 0.0036 0.0023 0.0009 0.0002 0.0003 0.0007 0.0002 0.0047 0.0002

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 9.4494 6.0647 4.5003 10.601 9.4243 8.2334 11.9355 11.0857 9.0272 12.5208
Translational Efficiency 0.8518 0.9671 1.4215 0.4621 0.8154 1.2201 0.4987 0.5816 0.9625 0.7238

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Transcription factor; involved in regulation of invasive growth and starch degradation; controls the activation of FLO11 and STA2 in response to nutritional signals; forms a heterodimer with Flo8p that interacts with the Swi/Snf complex during transcriptional activation of FLO1, FLO11, and STA1
Localization
Cell Percentages cytoplasm (18%), nucleus (8%), mixed (48%)
Cell Cycle Regulation No
Subcompartmental Group N/A

Mss11

Mss11


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Mss11-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available