Standard name | |
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Human Ortholog | |
Description | Mg2+-dependent phosphatidate (PA) phosphatase; dephosphorylates PA to yield diacylglycerol; regulates phospholipid synthesis, nuclear/ER membrane growth, lipid droplet formation, triacylglycerol synthesis, vacuolar homeostasis and cell wall integrity; phosphorylated by Pho85p/Pho80p, Cdc28p/Cyclin B, PKA, PKC, and CKII, regulating activity, localization, and proteosomal degradation; homolog of mammalian lipins 1 and 2; human homologs LPIN1, LPIN2, LPIN3 complement the null |
ensLOC | DeepLoc | |||||||||||||||||||||||
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Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 1 | 0 | 3 | 0 | 1 | 0 | 0 | 1 | 3 | – | 1 | 2 | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 0 | 2 | 0 | 4 | 4 |
Bud | 4 | 0 | 1 | 0 | 1 | 4 | 3 | 2 | 9 | – | 3 | 12 | 9 | 4 | 3 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 5 | 6 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 2 | 2 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 3 | 10 | 7 | 4 | 4 | 9 | 10 | 11 | 9 | – | 3 | 6 | 10 | 11 | 8 | 11 | 10 | 1 | 0 | 0 | 0 | 0 | 0 | 1 |
Cytoplasm | 114 | 298 | 236 | 155 | 222 | 282 | 349 | 326 | 244 | – | 130 | 182 | 329 | 488 | 512 | 89 | 78 | 17 | 99 | 306 | 214 | 129 | 372 | 360 |
Endoplasmic Reticulum | 1 | 7 | 4 | 8 | 8 | 3 | 11 | 5 | 3 | – | 0 | 1 | 0 | 2 | 8 | 5 | 4 | 2 | 7 | 1 | 8 | 19 | 45 | 56 |
Endosome | 1 | 0 | 7 | 5 | 4 | 7 | 13 | 13 | 4 | – | 1 | 3 | 1 | 4 | 6 | 2 | 0 | 0 | 1 | 1 | 18 | 3 | 7 | 13 |
Golgi | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | – | 1 | 0 | 0 | 0 | 0 | 0 | 3 | 0 | 1 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 6 | 1 | 1 | 2 | 2 | 6 | 15 | 8 | 56 | – | 60 | 50 | 5 | 0 | 1 | 1 | 1 | 1 | 2 | 0 | 6 | 6 | 15 | 12 |
Nucleus | 0 | 2 | 1 | 0 | 0 | 1 | 0 | 4 | 2 | – | 2 | 1 | 0 | 1 | 4 | 0 | 1 | 0 | 0 | 0 | 1 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 1 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 1 | – | 0 | 0 | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 1 | 3 | 1 | 2 | 0 | – | 0 | 1 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 0 | 2 | 0 | 3 | 5 |
Vac/Vac Membrane | 1 | 0 | 10 | 5 | 4 | 9 | 13 | 17 | 6 | – | 6 | 12 | 0 | 8 | 2 | 3 | 5 | 0 | 0 | 6 | 8 | 7 | 22 | 21 |
Unique Cell Count | 121 | 306 | 254 | 170 | 237 | 308 | 382 | 360 | 296 | 180 | 234 | 335 | 501 | 527 | 98 | 88 | 17 | 119 | 318 | 269 | 172 | 490 | 493 | |
Labelled Cell Count | 131 | 318 | 270 | 179 | 248 | 327 | 415 | 390 | 337 | 208 | 270 | 355 | 518 | 546 | 113 | 102 | 21 | 119 | 318 | 269 | 172 | 490 | 493 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
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Mean Cell GFP Intensity (1e-4) | 6.8 | 7.7 | 7.9 | 7.2 | 7.6 | 6.4 | 6.6 | 7.1 | 5.6 | – | 5.3 | 6.0 | 7.5 | 8.1 | 7.9 | 13.6 | 13.8 | 11.5 | 6.6 | 7.0 | 7.8 |
Std Deviation (1e-4) | 1.1 | 1.0 | 1.7 | 1.6 | 1.7 | 1.4 | 1.4 | 1.6 | 1.2 | – | 1.5 | 1.3 | 1.2 | 1.3 | 1.8 | 3.0 | 3.1 | 2.4 | 0.9 | 1.9 | 1.8 |
Intensity Change (Log2) | – | – | – | -0.13 | -0.05 | -0.3 | -0.26 | -0.16 | -0.51 | – | -0.57 | -0.4 | -0.08 | 0.04 | 0.0 | 0.78 | 0.8 | 0.54 | -0.26 | -0.18 | -0.03 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 3.2 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | -0.8 | 0.1 | -0.1 | 0.2 | 0.2 | – | 0 | -0.1 | 0.2 | -0.5 | 0 | 3.2 | 0 | 0 |
Cytoplasm | -0.7 | 0.3 | -0.6 | -0.7 | -1.0 | -3.7 | – | -5.8 | -4.8 | 3.2 | 2.9 | 2.8 | -0.7 | -1.3 | 1.1 |
Endoplasmic Reticulum | 0 | 1.3 | 0 | 1.1 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | -0.8 | -0.4 | 0.5 | 0.6 | -1.2 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 0 | 2.8 | 0 | 7.1 | – | 9.7 | 7.6 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | -0.5 | -1.5 | -0.7 | -0.4 | 0.5 | -1.3 | – | -0.3 | 0.6 | 0 | -2.0 | 0 | 0 | 0 | 0 |
R1 | R2 | R3 | ||||||||||||||||
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G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 3.2227 | 4.5393 | 4.0484 | 3.703 | 3.6531 | 3.9885 | 2.4388 | 3.4027 | 3.01 | 2.6225 | 2.9782 | 2.9886 | 2.3433 | 3.2629 | 2.7876 | 2.7318 | 2.3124 | 2.7476 |
Actin | 0.0386 | 0.0002 | 0.007 | 0.0223 | 0.0003 | 0.0044 | 0.019 | 0.0002 | 0.015 | 0.0074 | 0.0311 | 0.002 | 0.0239 | 0.0104 | 0.0142 | 0.0105 | 0.0184 | 0.0172 |
Bud | 0.0006 | 0.0002 | 0.0006 | 0.0038 | 0.0001 | 0.0001 | 0.0046 | 0.0006 | 0.0008 | 0.0128 | 0.0024 | 0.0009 | 0.011 | 0.0238 | 0.027 | 0.0374 | 0.03 | 0.0139 |
Bud Neck | 0.0031 | 0.0001 | 0.0093 | 0.0031 | 0.0004 | 0.0008 | 0.0028 | 0.0002 | 0.0007 | 0.001 | 0.0023 | 0.0032 | 0.0044 | 0.0047 | 0.0019 | 0.0009 | 0.0017 | 0.0065 |
Bud Periphery | 0.0012 | 0.0001 | 0.0015 | 0.0041 | 0.0001 | 0.0001 | 0.0034 | 0.0002 | 0.0006 | 0.0086 | 0.0027 | 0.0015 | 0.0091 | 0.0143 | 0.0239 | 0.0155 | 0.0369 | 0.0183 |
Bud Site | 0.0088 | 0.0014 | 0.0042 | 0.0299 | 0.0002 | 0.0002 | 0.0129 | 0.0048 | 0.0022 | 0.0061 | 0.0091 | 0.0005 | 0.0297 | 0.0652 | 0.0527 | 0.0158 | 0.005 | 0.0026 |
Cell Periphery | 0.0039 | 0.001 | 0.0007 | 0.0013 | 0.0003 | 0.0001 | 0.001 | 0.0001 | 0.0002 | 0.0002 | 0.0004 | 0.0001 | 0.0015 | 0.0018 | 0.0016 | 0.001 | 0.0011 | 0.0009 |
Cytoplasm | 0.6532 | 0.8698 | 0.7604 | 0.8339 | 0.8969 | 0.9168 | 0.7366 | 0.9333 | 0.8622 | 0.8582 | 0.6873 | 0.8963 | 0.6961 | 0.7611 | 0.7541 | 0.7573 | 0.6234 | 0.8036 |
Cytoplasmic Foci | 0.022 | 0.0059 | 0.0162 | 0.0136 | 0.0058 | 0.0088 | 0.039 | 0.0176 | 0.0192 | 0.0265 | 0.0361 | 0.0243 | 0.0337 | 0.0211 | 0.0203 | 0.0326 | 0.0608 | 0.0257 |
Eisosomes | 0.0023 | 0 | 0.0003 | 0.0004 | 0.0001 | 0.0002 | 0.0031 | 0 | 0.0001 | 0.0001 | 0.0002 | 0 | 0.0003 | 0.0001 | 0.0002 | 0.0001 | 0.0001 | 0.0001 |
Endoplasmic Reticulum | 0.02 | 0.0038 | 0.0073 | 0.0063 | 0.0022 | 0.0041 | 0.0114 | 0.0024 | 0.0033 | 0.0024 | 0.0029 | 0.0068 | 0.0107 | 0.0037 | 0.006 | 0.0041 | 0.0031 | 0.0105 |
Endosome | 0.0289 | 0.005 | 0.0119 | 0.0118 | 0.0038 | 0.0095 | 0.0473 | 0.0112 | 0.0378 | 0.0227 | 0.0528 | 0.0337 | 0.0251 | 0.0245 | 0.0146 | 0.0264 | 0.0415 | 0.0131 |
Golgi | 0.0098 | 0.0006 | 0.0079 | 0.0026 | 0.0003 | 0.0012 | 0.0147 | 0.001 | 0.0137 | 0.0084 | 0.0629 | 0.0056 | 0.0034 | 0.0015 | 0.0011 | 0.0014 | 0.0116 | 0.0013 |
Lipid Particles | 0.0191 | 0.0002 | 0.0084 | 0.0012 | 0.0002 | 0.0022 | 0.0135 | 0.0002 | 0.0019 | 0.0047 | 0.007 | 0.0008 | 0.0032 | 0.0005 | 0.0007 | 0.0008 | 0.0085 | 0.0006 |
Mitochondria | 0.0054 | 0.0002 | 0.0328 | 0.003 | 0.0002 | 0.0008 | 0.0151 | 0.0002 | 0.0036 | 0.012 | 0.0638 | 0.0054 | 0.0024 | 0.0008 | 0.0017 | 0.0017 | 0.0029 | 0.0011 |
None | 0.1404 | 0.1067 | 0.1135 | 0.0491 | 0.0865 | 0.0431 | 0.0365 | 0.0228 | 0.0274 | 0.0126 | 0.009 | 0.009 | 0.118 | 0.0524 | 0.0602 | 0.0619 | 0.1284 | 0.0723 |
Nuclear Periphery | 0.0047 | 0.0005 | 0.0011 | 0.0007 | 0.0002 | 0.0014 | 0.0084 | 0.0004 | 0.0009 | 0.0008 | 0.0019 | 0.0004 | 0.0059 | 0.0014 | 0.0022 | 0.0043 | 0.0031 | 0.0018 |
Nucleolus | 0.0029 | 0.0001 | 0.0003 | 0.0001 | 0.0001 | 0.0002 | 0.0006 | 0 | 0 | 0.0002 | 0.0003 | 0.0001 | 0.0013 | 0.0004 | 0.0009 | 0.0007 | 0.0005 | 0.0003 |
Nucleus | 0.0024 | 0.0008 | 0.001 | 0.0011 | 0.0004 | 0.0008 | 0.0015 | 0.0011 | 0.0009 | 0.0012 | 0.0018 | 0.0007 | 0.0047 | 0.0031 | 0.0044 | 0.0122 | 0.003 | 0.003 |
Peroxisomes | 0.0197 | 0 | 0.0101 | 0.0013 | 0.0001 | 0.0014 | 0.0139 | 0.0004 | 0.0022 | 0.0073 | 0.0171 | 0.0051 | 0.0009 | 0.0004 | 0.0009 | 0.0007 | 0.0045 | 0.0006 |
Punctate Nuclear | 0.0056 | 0.0002 | 0.0007 | 0.0003 | 0.0002 | 0.0012 | 0.0018 | 0.0004 | 0.0004 | 0.0007 | 0.0007 | 0.0003 | 0.0053 | 0.0016 | 0.0042 | 0.0032 | 0.0105 | 0.0029 |
Vacuole | 0.0063 | 0.003 | 0.004 | 0.0092 | 0.0015 | 0.0022 | 0.0102 | 0.0024 | 0.0055 | 0.005 | 0.0062 | 0.0025 | 0.0083 | 0.0063 | 0.0064 | 0.0097 | 0.0039 | 0.0033 |
Vacuole Periphery | 0.0009 | 0.0002 | 0.0007 | 0.0008 | 0.0001 | 0.0004 | 0.0025 | 0.0003 | 0.0016 | 0.0012 | 0.0018 | 0.0006 | 0.0011 | 0.001 | 0.0009 | 0.0019 | 0.001 | 0.0006 |
R1 | R2 | |||||||||
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G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 12.5726 | 11.0486 | 14.4182 | 15.3157 | 14.6432 | 13.6305 | 13.001 | 13.1297 | 14.5215 | 15.9062 |
Translational Efficiency | 1.1453 | 0.8622 | 0.74 | 0.7666 | 0.7419 | 0.7739 | 0.804 | 0.6053 | 0.7313 | 0.6473 |
Dataset | Hit |
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Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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