Standard name
Human Ortholog
Description Mg2+-dependent phosphatidate (PA) phosphatase; dephosphorylates PA to yield diacylglycerol; regulates phospholipid synthesis, nuclear/ER membrane growth, lipid droplet formation, triacylglycerol synthesis, vacuolar homeostasis and cell wall integrity; phosphorylated by Pho85p/Pho80p, Cdc28p/Cyclin B, PKA, PKC, and CKII, regulating activity, localization, and proteosomal degradation; homolog of mammalian lipins 1 and 2; human homologs LPIN1, LPIN2, LPIN3 complement the null

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.11 0.11 0.06 0 0 0 0 0 0
Cytoplasm 0.94 0.97 0.93 0.91 0.94 0.92 0.91 0.91 0.82 0.72 0.78 0.98 0.97 0.97 0.91 0.89 1.0 0.84 0.97 0.8 0.75 0.76 0.73
Endoplasmic Reticulum 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0.05 0.05 0.12 0.06 0 0 0.11 0.09 0.11
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.05 0 0 0 0 0 0 0 0.19 0.33 0.21 0 0 0 0 0 0.06 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0.05 0 0 0.05 0 0 0 0 0.06 0 0 0 0 0.05 0.05 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 1 0 3 0 1 0 0 1 3 1 2 0 0 1 0 0 0 1 0 2 0 4 4
Bud 4 0 1 0 1 4 3 2 9 3 12 9 4 3 0 0 0 0 0 1 0 5 6
Bud Neck 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 1 0 2 2
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 3 10 7 4 4 9 10 11 9 3 6 10 11 8 11 10 1 0 0 0 0 0 1
Cytoplasm 114 298 236 155 222 282 349 326 244 130 182 329 488 512 89 78 17 99 306 214 129 372 360
Endoplasmic Reticulum 1 7 4 8 8 3 11 5 3 0 1 0 2 8 5 4 2 7 1 8 19 45 56
Endosome 1 0 7 5 4 7 13 13 4 1 3 1 4 6 2 0 0 1 1 18 3 7 13
Golgi 0 0 0 0 1 0 0 0 0 1 0 0 0 0 0 3 0 1 0 0 0 0 0
Mitochondria 6 1 1 2 2 6 15 8 56 60 50 5 0 1 1 1 1 2 0 6 6 15 12
Nucleus 0 2 1 0 0 1 0 4 2 2 1 0 1 4 0 1 0 0 0 1 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 1 0 0 0 1 0 0 0 0 0 0 0 1 0
Nucleolus 0 0 0 0 0 2 0 0 1 0 0 1 0 0 1 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0
SpindlePole 0 0 0 0 1 3 1 2 0 0 1 0 0 0 1 0 0 1 0 2 0 3 5
Vac/Vac Membrane 1 0 10 5 4 9 13 17 6 6 12 0 8 2 3 5 0 0 6 8 7 22 21
Unique Cell Count 121 306 254 170 237 308 382 360 296 180 234 335 501 527 98 88 17 119 318 269 172 490 493
Labelled Cell Count 131 318 270 179 248 327 415 390 337 208 270 355 518 546 113 102 21 119 318 269 172 490 493


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 6.8 7.7 7.9 7.2 7.6 6.4 6.6 7.1 5.6 5.3 6.0 7.5 8.1 7.9 13.6 13.8 11.5 6.6 7.0 7.8
Std Deviation (1e-4) 1.1 1.0 1.7 1.6 1.7 1.4 1.4 1.6 1.2 1.5 1.3 1.2 1.3 1.8 3.0 3.1 2.4 0.9 1.9 1.8
Intensity Change (Log2) -0.13 -0.05 -0.3 -0.26 -0.16 -0.51 -0.57 -0.4 -0.08 0.04 0.0 0.78 0.8 0.54 -0.26 -0.18 -0.03


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 3.2 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 -0.8 0.1 -0.1 0.2 0.2 0 -0.1 0.2 -0.5 0 3.2 0 0
Cytoplasm -0.7 0.3 -0.6 -0.7 -1.0 -3.7 -5.8 -4.8 3.2 2.9 2.8 -0.7 -1.3 1.1
Endoplasmic Reticulum 0 1.3 0 1.1 0 0 0 0 0 0 0 0 0 0
Endosome 0 -0.8 -0.4 0.5 0.6 -1.2 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 2.8 0 7.1 9.7 7.6 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole -0.5 -1.5 -0.7 -0.4 0.5 -1.3 -0.3 0.6 0 -2.0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 3.2227 4.5393 4.0484 3.703 3.6531 3.9885 2.4388 3.4027 3.01 2.6225 2.9782 2.9886 2.3433 3.2629 2.7876 2.7318 2.3124 2.7476
Actin 0.0386 0.0002 0.007 0.0223 0.0003 0.0044 0.019 0.0002 0.015 0.0074 0.0311 0.002 0.0239 0.0104 0.0142 0.0105 0.0184 0.0172
Bud 0.0006 0.0002 0.0006 0.0038 0.0001 0.0001 0.0046 0.0006 0.0008 0.0128 0.0024 0.0009 0.011 0.0238 0.027 0.0374 0.03 0.0139
Bud Neck 0.0031 0.0001 0.0093 0.0031 0.0004 0.0008 0.0028 0.0002 0.0007 0.001 0.0023 0.0032 0.0044 0.0047 0.0019 0.0009 0.0017 0.0065
Bud Periphery 0.0012 0.0001 0.0015 0.0041 0.0001 0.0001 0.0034 0.0002 0.0006 0.0086 0.0027 0.0015 0.0091 0.0143 0.0239 0.0155 0.0369 0.0183
Bud Site 0.0088 0.0014 0.0042 0.0299 0.0002 0.0002 0.0129 0.0048 0.0022 0.0061 0.0091 0.0005 0.0297 0.0652 0.0527 0.0158 0.005 0.0026
Cell Periphery 0.0039 0.001 0.0007 0.0013 0.0003 0.0001 0.001 0.0001 0.0002 0.0002 0.0004 0.0001 0.0015 0.0018 0.0016 0.001 0.0011 0.0009
Cytoplasm 0.6532 0.8698 0.7604 0.8339 0.8969 0.9168 0.7366 0.9333 0.8622 0.8582 0.6873 0.8963 0.6961 0.7611 0.7541 0.7573 0.6234 0.8036
Cytoplasmic Foci 0.022 0.0059 0.0162 0.0136 0.0058 0.0088 0.039 0.0176 0.0192 0.0265 0.0361 0.0243 0.0337 0.0211 0.0203 0.0326 0.0608 0.0257
Eisosomes 0.0023 0 0.0003 0.0004 0.0001 0.0002 0.0031 0 0.0001 0.0001 0.0002 0 0.0003 0.0001 0.0002 0.0001 0.0001 0.0001
Endoplasmic Reticulum 0.02 0.0038 0.0073 0.0063 0.0022 0.0041 0.0114 0.0024 0.0033 0.0024 0.0029 0.0068 0.0107 0.0037 0.006 0.0041 0.0031 0.0105
Endosome 0.0289 0.005 0.0119 0.0118 0.0038 0.0095 0.0473 0.0112 0.0378 0.0227 0.0528 0.0337 0.0251 0.0245 0.0146 0.0264 0.0415 0.0131
Golgi 0.0098 0.0006 0.0079 0.0026 0.0003 0.0012 0.0147 0.001 0.0137 0.0084 0.0629 0.0056 0.0034 0.0015 0.0011 0.0014 0.0116 0.0013
Lipid Particles 0.0191 0.0002 0.0084 0.0012 0.0002 0.0022 0.0135 0.0002 0.0019 0.0047 0.007 0.0008 0.0032 0.0005 0.0007 0.0008 0.0085 0.0006
Mitochondria 0.0054 0.0002 0.0328 0.003 0.0002 0.0008 0.0151 0.0002 0.0036 0.012 0.0638 0.0054 0.0024 0.0008 0.0017 0.0017 0.0029 0.0011
None 0.1404 0.1067 0.1135 0.0491 0.0865 0.0431 0.0365 0.0228 0.0274 0.0126 0.009 0.009 0.118 0.0524 0.0602 0.0619 0.1284 0.0723
Nuclear Periphery 0.0047 0.0005 0.0011 0.0007 0.0002 0.0014 0.0084 0.0004 0.0009 0.0008 0.0019 0.0004 0.0059 0.0014 0.0022 0.0043 0.0031 0.0018
Nucleolus 0.0029 0.0001 0.0003 0.0001 0.0001 0.0002 0.0006 0 0 0.0002 0.0003 0.0001 0.0013 0.0004 0.0009 0.0007 0.0005 0.0003
Nucleus 0.0024 0.0008 0.001 0.0011 0.0004 0.0008 0.0015 0.0011 0.0009 0.0012 0.0018 0.0007 0.0047 0.0031 0.0044 0.0122 0.003 0.003
Peroxisomes 0.0197 0 0.0101 0.0013 0.0001 0.0014 0.0139 0.0004 0.0022 0.0073 0.0171 0.0051 0.0009 0.0004 0.0009 0.0007 0.0045 0.0006
Punctate Nuclear 0.0056 0.0002 0.0007 0.0003 0.0002 0.0012 0.0018 0.0004 0.0004 0.0007 0.0007 0.0003 0.0053 0.0016 0.0042 0.0032 0.0105 0.0029
Vacuole 0.0063 0.003 0.004 0.0092 0.0015 0.0022 0.0102 0.0024 0.0055 0.005 0.0062 0.0025 0.0083 0.0063 0.0064 0.0097 0.0039 0.0033
Vacuole Periphery 0.0009 0.0002 0.0007 0.0008 0.0001 0.0004 0.0025 0.0003 0.0016 0.0012 0.0018 0.0006 0.0011 0.001 0.0009 0.0019 0.001 0.0006

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 12.5726 11.0486 14.4182 15.3157 14.6432 13.6305 13.001 13.1297 14.5215 15.9062
Translational Efficiency 1.1453 0.8622 0.74 0.7666 0.7419 0.7739 0.804 0.6053 0.7313 0.6473

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency
Standard name
Human Ortholog
Description Mg2+-dependent phosphatidate (PA) phosphatase; dephosphorylates PA to yield diacylglycerol; regulates phospholipid synthesis, nuclear/ER membrane growth, lipid droplet formation, triacylglycerol synthesis, vacuolar homeostasis and cell wall integrity; phosphorylated by Pho85p/Pho80p, Cdc28p/Cyclin B, PKA, PKC, and CKII, regulating activity, localization, and proteosomal degradation; homolog of mammalian lipins 1 and 2; human homologs LPIN1, LPIN2, LPIN3 complement the null
Localization
Cell Percentages cytoplasm (97%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-4

Pah1

Pah1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)

Molecular Function (Gene Ontology)

Cellular Component (Gene Ontology)


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Pah1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available