Standard name | |
---|---|
Human Ortholog | |
Description | Protein required for mismatch repair in mitosis and meiosis; also required for crossing over during meiosis; forms a complex with Pms1p and Msh2p-Msh3p during mismatch repair; human homolog is associated with hereditary non-polyposis colon cancer |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 1 | 1 | 3 | 0 | 0 | – | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 1 | – | 1 | 2 | 1 | 1 | 2 | 3 |
Bud | 0 | 2 | 0 | 8 | 4 | 4 | 9 | – | 11 | 18 | 13 | 22 | 0 | 1 | 4 | 2 | 2 | – | 1 | 3 | 2 | 2 | 2 | 4 |
Bud Neck | 4 | 4 | 18 | 8 | 24 | 11 | 19 | – | 8 | 7 | 9 | 11 | 0 | 1 | 0 | 11 | 10 | – | 0 | 2 | 2 | 0 | 3 | 5 |
Bud Site | 0 | 0 | 3 | 2 | 1 | 7 | 8 | – | 10 | 23 | 21 | 15 | 1 | 0 | 1 | 0 | 1 | – | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 1 | 0 | – | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 4 | 7 | – | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 25 | 72 | 102 | 62 | 72 | 73 | 96 | – | 55 | 39 | 39 | 46 | 35 | 44 | 65 | 495 | 422 | – | 24 | 48 | 46 | 23 | 21 | 21 |
Endoplasmic Reticulum | 5 | 6 | 1 | 0 | 4 | 2 | 0 | – | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 35 | 25 | – | 0 | 0 | 0 | 3 | 1 | 1 |
Endosome | 5 | 40 | 11 | 11 | 10 | 29 | 18 | – | 7 | 0 | 7 | 9 | 11 | 48 | 59 | 32 | 63 | – | 32 | 63 | 36 | 22 | 36 | 38 |
Golgi | 0 | 0 | 3 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 1 | – | 0 | 0 | 1 | 0 | 0 | 0 |
Mitochondria | 26 | 40 | 4 | 17 | 7 | 76 | 94 | – | 164 | 172 | 185 | 118 | 46 | 9 | 18 | 73 | 29 | – | 8 | 37 | 33 | 13 | 17 | 20 |
Nucleus | 8 | 21 | 12 | 14 | 6 | 18 | 19 | – | 15 | 25 | 18 | 28 | 10 | 5 | 8 | 8 | 5 | – | 9 | 25 | 9 | 4 | 2 | 0 |
Nuclear Periphery | 1 | 1 | 0 | 0 | 0 | 0 | 0 | – | 1 | 0 | 2 | 0 | 0 | 0 | 0 | 1 | 5 | – | 2 | 3 | 2 | 0 | 1 | 2 |
Nucleolus | 2 | 3 | 0 | 2 | 0 | 2 | 4 | – | 3 | 6 | 4 | 5 | 3 | 3 | 7 | 8 | 4 | – | 3 | 10 | 4 | 4 | 2 | 1 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 1 | 3 | – | 2 | 0 | 0 | 1 | 0 | 7 | 8 | 1 | 1 | – | 0 | 1 | 0 | 0 | 0 | 1 |
SpindlePole | 11 | 24 | 44 | 65 | 63 | 48 | 86 | – | 49 | 55 | 42 | 72 | 16 | 24 | 20 | 84 | 98 | – | 52 | 94 | 79 | 31 | 53 | 58 |
Vac/Vac Membrane | 13 | 50 | 9 | 4 | 5 | 5 | 8 | – | 5 | 2 | 6 | 3 | 6 | 13 | 16 | 36 | 34 | – | 12 | 42 | 30 | 27 | 23 | 23 |
Unique Cell Count | 87 | 229 | 189 | 168 | 173 | 224 | 286 | 257 | 244 | 256 | 236 | 110 | 126 | 172 | 664 | 597 | 160 | 359 | 276 | 143 | 177 | 190 | ||
Labelled Cell Count | 100 | 263 | 208 | 194 | 199 | 277 | 364 | 330 | 347 | 347 | 330 | 129 | 157 | 207 | 791 | 708 | 160 | 359 | 276 | 143 | 177 | 190 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 3.8 | 4.1 | 4.8 | 4.4 | 4.9 | 3.9 | 4.1 | – | 3.6 | 3.3 | 3.2 | 3.9 | 3.7 | 4.0 | 4.3 | 5.2 | 5.4 | 4.8 | 3.6 | 3.7 | 4.4 |
Std Deviation (1e-4) | 0.4 | 0.6 | 1.3 | 1.6 | 1.2 | 1.4 | 1.4 | – | 1.3 | 1.1 | 1.1 | 1.3 | 0.8 | 0.9 | 1.5 | 1.1 | 1.2 | 1.0 | 1.3 | 1.2 | 1.9 |
Intensity Change (Log2) | – | – | – | -0.13 | 0.01 | -0.31 | -0.25 | – | -0.44 | -0.54 | -0.58 | -0.31 | -0.38 | -0.28 | -0.17 | 0.11 | 0.16 | -0.02 | -0.41 | -0.39 | -0.14 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Actin | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | – |
Bud Neck | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | – |
Bud Site | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | – |
Cell Periphery | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | – |
Cyto | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endoplasmic Reticulum | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | – |
Endosome | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | – |
Golgi | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | – |
Mitochondria | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | – |
Nuclear Periphery | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | – |
Nuc | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleolus | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | – |
Peroxisomes | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | – |
SpindlePole | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | – |
Vac | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cortical Patches | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | – |
Cytoplasm | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | – |
Nucleus | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | – |
Vacuole | – | – | – | – | – | – | – | – | – | – | – | – | 0.4 | 0.5 | – |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 0.0527 | -0.3919 | -0.5816 | -0.5459 | -0.9043 | -0.639 | -0.4374 | -0.323 | -0.4093 | -0.9684 | -0.9442 | -0.5125 | 0.1095 | -0.2633 | -0.1763 | 0.029 | -0.0724 | -0.4711 |
Actin | 0.0359 | 0.0031 | 0.0173 | 0.0039 | 0.0009 | 0.0208 | 0.0147 | 0.0015 | 0.0242 | 0.0025 | 0.0006 | 0.0015 | 0.0153 | 0.0018 | 0.0112 | 0.0204 | 0.0026 | 0.004 |
Bud | 0.0008 | 0.0002 | 0.0001 | 0.0002 | 0.0001 | 0.0023 | 0.0005 | 0.0064 | 0.0003 | 0.0002 | 0.0001 | 0.0002 | 0.0007 | 0.0002 | 0.0003 | 0.0006 | 0.0003 | 0.0002 |
Bud Neck | 0.0021 | 0.0017 | 0.002 | 0.0031 | 0.001 | 0.0054 | 0.0024 | 0.0019 | 0.0016 | 0.0035 | 0.0026 | 0.0067 | 0.0036 | 0.0018 | 0.0027 | 0.0033 | 0.0014 | 0.0066 |
Bud Periphery | 0.0013 | 0.0004 | 0.0002 | 0.0003 | 0.0004 | 0.0056 | 0.0008 | 0.0012 | 0.0003 | 0.0002 | 0.0001 | 0.0002 | 0.0012 | 0.0002 | 0.0004 | 0.0013 | 0.001 | 0.0003 |
Bud Site | 0.0056 | 0.003 | 0.0013 | 0.0009 | 0.0002 | 0.001 | 0.0045 | 0.004 | 0.004 | 0.0007 | 0.0004 | 0.0005 | 0.0039 | 0.0032 | 0.0072 | 0.0116 | 0.0005 | 0.0004 |
Cell Periphery | 0.0003 | 0.0002 | 0.0001 | 0.0002 | 0.0002 | 0.0001 | 0.0004 | 0.0002 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0003 | 0.0001 | 0.0002 | 0.0008 | 0.0001 | 0.0001 |
Cytoplasm | 0.0561 | 0.0728 | 0.0537 | 0.169 | 0.0536 | 0.0472 | 0.0455 | 0.0675 | 0.035 | 0.0565 | 0.0747 | 0.0584 | 0.0626 | 0.0821 | 0.0519 | 0.1153 | 0.0794 | 0.0608 |
Cytoplasmic Foci | 0.0705 | 0.0477 | 0.0375 | 0.0526 | 0.0225 | 0.0418 | 0.0767 | 0.0601 | 0.0642 | 0.0255 | 0.0389 | 0.0271 | 0.0765 | 0.0593 | 0.0404 | 0.0331 | 0.0266 | 0.0195 |
Eisosomes | 0.0007 | 0.0001 | 0.0001 | 0.0002 | 0.0001 | 0.0002 | 0.0003 | 0.0001 | 0.0001 | 0 | 0 | 0.0001 | 0.0002 | 0.0001 | 0.0001 | 0.0014 | 0.0001 | 0 |
Endoplasmic Reticulum | 0.0097 | 0.0063 | 0.0056 | 0.0203 | 0.0106 | 0.0026 | 0.0044 | 0.0045 | 0.0041 | 0.0063 | 0.0133 | 0.0054 | 0.0066 | 0.0047 | 0.0044 | 0.0047 | 0.0052 | 0.0041 |
Endosome | 0.058 | 0.0336 | 0.0208 | 0.0321 | 0.0424 | 0.0144 | 0.0415 | 0.0264 | 0.0239 | 0.0161 | 0.0744 | 0.0254 | 0.0611 | 0.0314 | 0.0241 | 0.0224 | 0.0447 | 0.0182 |
Golgi | 0.0135 | 0.0031 | 0.0025 | 0.0029 | 0.002 | 0.0028 | 0.0067 | 0.0025 | 0.0044 | 0.0014 | 0.0024 | 0.0019 | 0.0118 | 0.0036 | 0.0028 | 0.0033 | 0.005 | 0.0018 |
Lipid Particles | 0.0518 | 0.0393 | 0.0215 | 0.0073 | 0.0354 | 0.0392 | 0.0269 | 0.0346 | 0.0348 | 0.0071 | 0.0286 | 0.0209 | 0.0654 | 0.0259 | 0.0212 | 0.0228 | 0.0094 | 0.0117 |
Mitochondria | 0.0326 | 0.0041 | 0.004 | 0.0029 | 0.0061 | 0.0015 | 0.0018 | 0.0016 | 0.0012 | 0.0012 | 0.0005 | 0.001 | 0.0337 | 0.0014 | 0.0008 | 0.0069 | 0.0235 | 0.0009 |
None | 0.115 | 0.1812 | 0.117 | 0.2583 | 0.1757 | 0.1453 | 0.0449 | 0.1434 | 0.0568 | 0.0288 | 0.1684 | 0.0999 | 0.1364 | 0.1575 | 0.1459 | 0.0572 | 0.1797 | 0.0809 |
Nuclear Periphery | 0.0597 | 0.0515 | 0.0925 | 0.0512 | 0.1678 | 0.0704 | 0.0664 | 0.0606 | 0.0595 | 0.0709 | 0.0423 | 0.0594 | 0.0584 | 0.0308 | 0.0851 | 0.0238 | 0.0535 | 0.0439 |
Nucleolus | 0.0086 | 0.0239 | 0.0201 | 0.0203 | 0.0075 | 0.0181 | 0.0081 | 0.0254 | 0.0195 | 0.0143 | 0.0793 | 0.0294 | 0.0095 | 0.0248 | 0.0223 | 0.0236 | 0.0054 | 0.0208 |
Nucleus | 0.0738 | 0.1785 | 0.1619 | 0.2576 | 0.1294 | 0.2419 | 0.1285 | 0.13 | 0.1094 | 0.3286 | 0.2373 | 0.2606 | 0.0722 | 0.1375 | 0.1291 | 0.3789 | 0.2798 | 0.2024 |
Peroxisomes | 0.0274 | 0.0028 | 0.0061 | 0.0011 | 0.0016 | 0.0103 | 0.0046 | 0.0046 | 0.0057 | 0.0065 | 0.0057 | 0.0014 | 0.0164 | 0.0046 | 0.0054 | 0.0208 | 0.0018 | 0.0015 |
Punctate Nuclear | 0.3704 | 0.3402 | 0.4312 | 0.1086 | 0.3235 | 0.3258 | 0.5158 | 0.418 | 0.5481 | 0.4218 | 0.22 | 0.396 | 0.3411 | 0.4226 | 0.4384 | 0.2439 | 0.2672 | 0.5172 |
Vacuole | 0.0033 | 0.0051 | 0.0033 | 0.0053 | 0.011 | 0.0021 | 0.003 | 0.0036 | 0.0018 | 0.0063 | 0.0079 | 0.0028 | 0.0165 | 0.0046 | 0.0047 | 0.0028 | 0.0065 | 0.0035 |
Vacuole Periphery | 0.0026 | 0.0014 | 0.0012 | 0.0019 | 0.008 | 0.0009 | 0.0016 | 0.0018 | 0.0009 | 0.0016 | 0.0023 | 0.0013 | 0.0066 | 0.0017 | 0.0014 | 0.0011 | 0.006 | 0.0011 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 6.9261 | 4.697 | 5.0697 | 8.218 | 8.0511 | 5.2514 | 9.8221 | 8.5962 | 6.7687 | 9.0152 |
Translational Efficiency | 0.8608 | 0.6515 | 1.1311 | 0.6986 | 0.6071 | 0.9348 | 0.5704 | 0.5222 | 0.8725 | 0.6437 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
---|
Gene | Images |
---|