Standard name
Human Ortholog
Description Essential kinetochore protein; component of the CBF3 complex that binds the CDEIII region of the centromere; contains an N-terminal Zn2Cys6 type zinc finger domain, a C-terminal acidic domain, and a putative coiled coil dimerization domain
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0.06 0.09 0.1 0.11 0.08 0.05 0.05 0.05 0.06 0.05 0.05 – 0.17 0.26 0.09 0.12 0.07 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Endosome 0.31 0.25 0.18 0.2 0.17 0.13 0.14 0.08 0.13 0.07 0.05 0.11 – 0.21 0.19 0.2 0.25 0.28 0.16 0.16 0.13 0.22 0.21 0.21
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.1 0.12 0 0.06 0 0.13 0.13 0.12 0.32 0.21 0.42 0.32 – 0 0 0.1 0.06 0.08 0 0 0 0 0 0
Nucleus 0.22 0.23 0.27 0.18 0.22 0.23 0.2 0.21 0.2 0.26 0.17 0.22 – 0.29 0.3 0.36 0.25 0.3 0.1 0.13 0.1 0.1 0.14 0.09
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.4 0.35 0.29 0.37 0.42 0.44 0.47 0.42 0.41 0.43 0.48 0.42 – 0.25 0.18 0.34 0.41 0.41 0.07 0.17 0.08 0.09 0.13 0.12
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0.36 0.16 0.49 0.42 0.55 0.35 0.43 0.51 0.32 0.35 0.22 0.32 – 0.17 0.24 0.22 0.31 0.31 0.55 0.41 0.51 0.47 0.35 0.23
Vac/Vac Membrane 0.11 0.14 0.1 0.05 0 0 0 0 0 0 0 0 – 0 0 0.07 0.07 0.1 0 0 0.05 0 0 0.09
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 1 0 0 0 0 1 0 1 – 0 0 0 0 0 0 0 1 0 0 1
Bud 2 3 0 0 1 1 4 3 7 10 6 11 – 1 0 0 5 0 1 2 3 0 1 2
Bud Neck 0 0 2 0 2 0 2 2 3 7 2 3 – 0 0 0 1 0 0 3 1 0 1 1
Bud Site 0 1 0 1 1 4 8 11 10 9 13 9 – 0 0 1 0 1 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 1 – 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 6 15 14 16 24 21 16 24 15 15 17 14 – 4 29 23 25 12 1 2 4 0 1 3
Endoplasmic Reticulum 1 0 0 0 1 0 0 0 0 0 0 0 – 0 1 3 7 4 0 0 0 0 1 0
Endosome 50 65 29 31 36 35 47 38 36 19 17 31 – 5 22 49 51 49 33 53 28 11 26 26
Golgi 0 0 0 1 1 0 0 0 0 0 0 0 – 0 0 4 0 1 0 0 1 0 0 1
Mitochondria 16 30 1 9 1 34 42 58 89 58 139 89 – 0 2 25 13 14 5 5 2 1 2 4
Nucleus 35 60 43 28 47 60 65 106 55 70 56 63 – 7 34 89 51 53 20 42 21 5 17 11
Nuclear Periphery 0 2 0 0 1 1 2 5 0 0 1 4 – 0 0 4 1 0 0 1 1 0 0 1
Nucleolus 65 90 45 56 88 116 157 213 112 116 158 118 – 6 20 83 86 73 13 54 17 4 16 15
Peroxisomes 0 0 0 0 0 0 3 7 1 2 2 2 – 0 0 0 1 0 0 1 0 0 0 0
SpindlePole 58 41 77 64 115 93 144 254 87 94 72 90 – 4 27 53 65 56 112 136 113 23 44 29
Vac/Vac Membrane 18 35 15 8 7 5 5 10 7 3 2 7 – 1 5 18 14 17 8 13 11 1 5 11
Unique Cell Count 161 257 157 153 211 264 332 502 274 270 330 282 24 113 246 208 178 206 331 220 50 125 124
Labelled Cell Count 251 342 226 214 326 370 495 731 422 404 485 443 28 140 352 320 280 206 331 220 50 125 124


Microtubule

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.2 6.2 6.9 6.3 7.0 5.5 5.7 5.8 4.9 5.2 4.5 5.2 – 7.4 7.1 7.8 8.1 7.5 7.7 6.9 7.5
Std Deviation (1e-4) 0.7 1.0 1.3 1.8 2.0 1.3 1.6 1.1 1.3 1.4 1.3 2.5 – 1.2 1.8 1.7 1.8 1.8 1.7 1.5 2.4
Intensity Change (Log2) – – – -0.13 0.02 -0.31 -0.26 -0.25 -0.5 -0.4 -0.61 -0.39 – 0.11 0.04 0.19 0.24 0.13 0.16 0.01 0.12


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0
Cytoplasm 0.5 0.8 -0.3 -1.8 -1.9 -1.4 -1.3 -1.6 -1.6 – 0 3.7 0.1 0.9 -0.7
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0
Endosome 0.4 -0.4 -1.4 -1.2 -3.9 0 -3.6 0 -2.2 – 0 0.2 0.4 1.4 2.0
Golgi 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0
Mitochondria 0 0 4.4 4.4 4.2 0 6.0 0 7.7 – 0 0 3.8 2.8 3.2
Nucleus -1.9 -1.1 -1.1 -1.9 -1.6 -1.7 -0.3 -2.7 -1.2 – 0.2 0.5 1.8 -0.6 0.5
Nuclear Periphery 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0
Nucleolus 1.5 2.6 3.1 3.9 3.1 0 2.9 0 2.7 – -0.4 -2.1 1.1 2.5 2.4
Peroxisomes 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0
SpindlePole -1.3 1.0 -2.8 -1.2 0.3 0 -2.9 0 -3.5 – -3.0 -4.2 -5.8 -3.5 -3.3
Vacuole -1.5 -2.5 -3.6 -4.2 -4.3 -3.2 -4.2 -5.0 -3.3 – 0 -1.6 -0.8 -1.0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 2.497 2.5182 2.2575 1.752 1.8132 2.2237 1.8232 2.3145 1.946 1.4835 2.0107 2.2267 1.844 1.8378 1.7708 1.0427 2.1039 1.0524
Actin 0.005 0.0022 0.0058 0.0002 0.0014 0.0005 0.017 0.0006 0.0129 0.0007 0.0527 0.0061 0.0359 0.0005 0.064 0.0783 0.0009 0.001
Bud 0.0005 0.0003 0.0005 0.0001 0.0002 0 0.0002 0.0002 0.0002 0.0001 0.0006 0.0001 0.001 0.0004 0.0014 0.001 0.0026 0.0006
Bud Neck 0.0034 0.0014 0.0058 0.0007 0.0023 0.0031 0.0042 0.0024 0.0059 0.0275 0.0061 0.0052 0.0057 0.002 0.0043 0.0042 0.0061 0.0091
Bud Periphery 0.0013 0.0002 0.0004 0 0.0002 0.0001 0.0003 0.0001 0.0002 0.0001 0.0009 0.0001 0.0012 0.0002 0.0011 0.0011 0.0011 0.0005
Bud Site 0.0015 0.003 0.0027 0.0002 0.0007 0.0001 0.0013 0.0026 0.0033 0.0004 0.0026 0.0004 0.0156 0.0066 0.0331 0.0082 0.0057 0.0024
Cell Periphery 0.0003 0.0001 0.0002 0 0.0001 0 0.0001 0 0.0001 0 0.0001 0 0.0008 0.0001 0.0003 0.0002 0.0001 0.0001
Cytoplasm 0.0264 0.0859 0.016 0.0084 0.0235 0.0098 0.0425 0.0716 0.0197 0.0607 0.0162 0.0193 0.0363 0.1174 0.0123 0.065 0.2672 0.0455
Cytoplasmic Foci 0.0188 0.0068 0.004 0.0054 0.033 0.0022 0.0202 0.0112 0.0115 0.0069 0.0293 0.0031 0.0475 0.0168 0.008 0.0098 0.0274 0.0108
Eisosomes 0.0002 0.0001 0.0001 0 0.0001 0 0.0002 0 0.0001 0 0.001 0 0.0004 0 0.0002 0.0003 0 0.0001
Endoplasmic Reticulum 0.0026 0.0024 0.0008 0.0002 0.0016 0.0003 0.0019 0.0022 0.0014 0.0016 0.0056 0.0009 0.0093 0.0037 0.0006 0.0025 0.0032 0.0008
Endosome 0.0246 0.009 0.0028 0.0008 0.0375 0.0021 0.0139 0.0053 0.0097 0.0055 0.0271 0.0029 0.0508 0.007 0.0035 0.0082 0.0339 0.0037
Golgi 0.004 0.0006 0.0009 0.0002 0.0029 0.0004 0.0033 0.0003 0.0022 0.0004 0.0083 0.0005 0.0249 0.0005 0.0042 0.014 0.0017 0.0007
Lipid Particles 0.0156 0.0008 0.0039 0.0009 0.0169 0.0006 0.0119 0.0009 0.0096 0.0008 0.0494 0.0005 0.0224 0.0006 0.0023 0.018 0.0016 0.0046
Mitochondria 0.0096 0.0008 0.0008 0.0002 0.0131 0.0084 0.0119 0.0006 0.0014 0.0004 0.0079 0.0005 0.0071 0.0005 0.0016 0.0055 0.002 0.0011
None 0.0125 0.019 0.0101 0.0009 0.0387 0.0256 0.0257 0.0241 0.0169 0.0072 0.0043 0.0247 0.01 0.0117 0.0012 0.0037 0.0209 0.0025
Nuclear Periphery 0.0421 0.0205 0.0091 0.0136 0.0423 0.013 0.0143 0.0175 0.0126 0.0217 0.0338 0.0107 0.0232 0.0239 0.0052 0.0093 0.0305 0.0117
Nucleolus 0.0263 0.0342 0.0389 0.0155 0.0361 0.0436 0.0159 0.0261 0.0238 0.0395 0.028 0.041 0.0071 0.0194 0.0302 0.0127 0.015 0.0321
Nucleus 0.3171 0.5044 0.3631 0.437 0.2389 0.3038 0.3529 0.4513 0.384 0.3629 0.2958 0.4258 0.3543 0.4418 0.3973 0.3011 0.3289 0.4548
Peroxisomes 0.0049 0.0022 0.0009 0.0058 0.0129 0.0003 0.0063 0.0004 0.0056 0.0006 0.01 0.0005 0.0262 0.0004 0.0068 0.0107 0.0012 0.0009
Punctate Nuclear 0.478 0.3018 0.5325 0.5096 0.4936 0.5855 0.4543 0.3803 0.4778 0.4608 0.4172 0.4567 0.308 0.3435 0.4214 0.4448 0.2367 0.4149
Vacuole 0.0027 0.0039 0.0007 0.0002 0.0029 0.0004 0.0013 0.0017 0.0007 0.0018 0.0017 0.0006 0.0102 0.0026 0.0008 0.0007 0.0112 0.0017
Vacuole Periphery 0.0024 0.0007 0.0002 0.0001 0.0013 0.0001 0.0004 0.0004 0.0004 0.0003 0.0014 0.0002 0.0023 0.0005 0.0002 0.0003 0.002 0.0004

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 6.3414 7.0185 6.8105 9.3597 7.8304 1.8108 6.4141 8.474 7.9812 10.0179
Translational Efficiency – – – – – – – – – –

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✔
Translational Efficiency –
Standard name
Human Ortholog
Description Essential kinetochore protein; component of the CBF3 complex that binds the CDEIII region of the centromere; contains an N-terminal Zn2Cys6 type zinc finger domain, a C-terminal acidic domain, and a putative coiled coil dimerization domain
Localization
Cell Percentages spindle pole (16%), nucleolus (9%), mixed (46%)
Cell Cycle Regulation No
Subcompartmental Group N/A

Cep3

Cep3


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Cep3-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available