Standard name | |
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Human Ortholog | |
Description | Cytoplasmic aldehyde dehydrogenase; involved in beta-alanine synthesis; uses NAD+ as the preferred coenzyme; very similar to Ald2p; expression is induced by stress and repressed by glucose |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | 0 | 3 | 1 | 4 | 2 | 5 | 2 |
Bud | 2 | 2 | 2 | 1 | 2 | 2 | 0 | 3 | 8 | 2 | 5 | 7 | 0 | 0 | 0 | – | – | 0 | 1 | 1 | 0 | 4 | 8 | 8 |
Bud Neck | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 2 | 2 | 1 | 0 | 0 | 0 | 0 | 0 | – | – | 0 | 1 | 0 | 0 | 0 | 3 | 3 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 0 | 0 | 0 | – | – | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 2 | 2 | 0 | 1 | 1 | 1 | 0 | 1 | 2 | 2 | 5 | 0 | 0 | 1 | – | – | 4 | 1 | 0 | 2 | 0 | 2 | 3 |
Cytoplasm | 261 | 221 | 170 | 138 | 199 | 181 | 176 | 233 | 165 | 175 | 113 | 138 | 199 | 343 | 309 | – | – | 118 | 241 | 206 | 136 | 175 | 318 | 192 |
Endoplasmic Reticulum | 1 | 2 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 1 | – | – | 8 | 1 | 0 | 3 | 2 | 2 | 0 |
Endosome | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | – | – | 3 | 2 | 2 | 1 | 0 | 7 | 2 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | – | – | 0 | 0 | 2 | 1 | 0 | 2 | 1 |
Mitochondria | 50 | 22 | 9 | 17 | 19 | 55 | 107 | 95 | 115 | 105 | 114 | 129 | 3 | 3 | 2 | – | – | 7 | 27 | 14 | 14 | 7 | 29 | 18 |
Nucleus | 0 | 0 | 0 | 1 | 2 | 0 | 0 | 1 | 0 | 1 | 3 | 2 | 0 | 0 | 2 | – | – | 1 | 0 | 0 | 0 | 1 | 0 | 0 |
Nuclear Periphery | 0 | 1 | 0 | 0 | 1 | 0 | 0 | 1 | 1 | 2 | 3 | 3 | 0 | 0 | 0 | – | – | 0 | 0 | 0 | 0 | 0 | 0 | 1 |
Nucleolus | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | – | – | 0 | 1 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 4 | 4 | 1 | 2 | 1 | 0 | 0 | 0 | – | – | 0 | 1 | 2 | 1 | 2 | 5 | 2 |
Vac/Vac Membrane | 0 | 1 | 0 | 0 | 0 | 0 | 3 | 7 | 2 | 3 | 5 | 3 | 0 | 1 | 0 | – | – | 5 | 4 | 2 | 2 | 1 | 5 | 11 |
Unique Cell Count | 288 | 235 | 174 | 143 | 215 | 203 | 229 | 276 | 232 | 241 | 195 | 223 | 200 | 347 | 310 | 129 | 292 | 242 | 173 | 203 | 399 | 253 | ||
Labelled Cell Count | 315 | 251 | 183 | 157 | 228 | 241 | 289 | 346 | 300 | 293 | 247 | 289 | 203 | 350 | 315 | 146 | 292 | 242 | 173 | 203 | 399 | 253 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 3.2 | 3.4 | 3.8 | 3.4 | 3.4 | 2.9 | 2.4 | 3.0 | 2.8 | 2.6 | 2.7 | 2.5 | 3.8 | 3.9 | 4.4 | 4.2 | 4.8 | 5.2 | 3.8 | 3.9 | 4.7 |
Std Deviation (1e-4) | 0.5 | 0.7 | 1.1 | 1.1 | 1.1 | 1.0 | 0.7 | 1.1 | 1.7 | 1.1 | 1.1 | 1.5 | 1.1 | 1.2 | 2.0 | 1.9 | 1.5 | 1.5 | 1.4 | 1.3 | 1.8 |
Intensity Change (Log2) | – | – | – | -0.16 | -0.14 | -0.39 | -0.68 | -0.35 | -0.44 | -0.51 | -0.5 | -0.59 | -0.01 | 0.06 | 0.21 | 0.17 | 0.36 | 0.47 | -0.0 | 0.03 | 0.31 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Actin | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud Neck | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud Site | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cell Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cyto | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endoplasmic Reticulum | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endosome | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Golgi | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Mitochondria | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nuclear Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nuc | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleolus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Peroxisomes | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
SpindlePole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Vac | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cortical Patches | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cytoplasm | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Vacuole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 2.0687 | 0.748 | 1.5124 | 1.7354 | 1.1645 | 1.7449 | 0.197 | 0.621 | 0.607 | 0.6334 | 0.631 | 0.4643 | 1.021 | 1.4534 | 0.9667 | 1.641 | 1.9807 | 0.7212 |
Actin | 0.0419 | 0.0001 | 0.0003 | 0.0002 | 0.0002 | 0.0004 | 0.0468 | 0.0069 | 0.0123 | 0.0382 | 0.0126 | 0.009 | 0.1084 | 0.0003 | 0.0179 | 0.041 | 0.1829 | 0.0119 |
Bud | 0.014 | 0 | 0.0001 | 0.0001 | 0 | 0.0001 | 0.0018 | 0.0046 | 0.0023 | 0.0003 | 0.0012 | 0.0007 | 0.0015 | 0.0029 | 0.0146 | 0.0002 | 0.0017 | 0.0002 |
Bud Neck | 0.0008 | 0 | 0.0001 | 0.0001 | 0.0002 | 0.0003 | 0.0039 | 0.0001 | 0.0005 | 0.0001 | 0.0015 | 0.0006 | 0.0036 | 0.0002 | 0.0002 | 0.001 | 0.0395 | 0.0003 |
Bud Periphery | 0.0283 | 0 | 0 | 0.0001 | 0 | 0.0001 | 0.0042 | 0.0178 | 0.0029 | 0.0003 | 0.0028 | 0.0015 | 0.003 | 0.0019 | 0.0059 | 0.0003 | 0.0025 | 0.0004 |
Bud Site | 0.0075 | 0 | 0.0001 | 0 | 0.0001 | 0 | 0.0043 | 0.0009 | 0.0112 | 0.0002 | 0.0069 | 0.0002 | 0.005 | 0.0075 | 0.0027 | 0.0014 | 0.0184 | 0.0001 |
Cell Periphery | 0.0006 | 0.0001 | 0 | 0 | 0.0001 | 0.0001 | 0.0006 | 0.0002 | 0.0003 | 0.0001 | 0.0007 | 0.0001 | 0.0002 | 0.0005 | 0.0002 | 0.0003 | 0.0007 | 0 |
Cytoplasm | 0.0088 | 0.2186 | 0.1799 | 0.2411 | 0.4413 | 0.2471 | 0.0849 | 0.28 | 0.1342 | 0.1803 | 0.244 | 0.1374 | 0.1145 | 0.23 | 0.1614 | 0.309 | 0.0662 | 0.2092 |
Cytoplasmic Foci | 0.0084 | 0.0006 | 0.0046 | 0.0027 | 0.0021 | 0.0202 | 0.0286 | 0.0037 | 0.0168 | 0.0118 | 0.0176 | 0.0088 | 0.0242 | 0.0052 | 0.015 | 0.0135 | 0.0462 | 0.0139 |
Eisosomes | 0.0008 | 0.0002 | 0.0001 | 0.0001 | 0 | 0.0001 | 0.001 | 0.0002 | 0.0004 | 0.0003 | 0.0003 | 0.0004 | 0.0005 | 0.0002 | 0.0004 | 0.0005 | 0.0009 | 0.0001 |
Endoplasmic Reticulum | 0.0483 | 0.0004 | 0.0004 | 0.0008 | 0.0025 | 0.0017 | 0.0049 | 0.0012 | 0.0036 | 0.0011 | 0.0033 | 0.0006 | 0.0011 | 0.0006 | 0.0018 | 0.0016 | 0.0012 | 0.0006 |
Endosome | 0.0268 | 0 | 0.0012 | 0.0005 | 0.0007 | 0.0009 | 0.0105 | 0.0006 | 0.0033 | 0.0049 | 0.0148 | 0.0006 | 0.0061 | 0.0001 | 0.004 | 0.0134 | 0.0168 | 0.0011 |
Golgi | 0.0077 | 0 | 0.0019 | 0.0001 | 0.0001 | 0.0001 | 0.0049 | 0.0003 | 0.0013 | 0.0014 | 0.009 | 0.0001 | 0.0053 | 0 | 0.0029 | 0.0065 | 0.0376 | 0.0013 |
Lipid Particles | 0.0029 | 0 | 0.0109 | 0.0002 | 0.0001 | 0.0009 | 0.0059 | 0.0004 | 0.0037 | 0.0006 | 0.016 | 0.0001 | 0.0017 | 0.0001 | 0.0061 | 0.0255 | 0.105 | 0.0004 |
Mitochondria | 0.0147 | 0 | 0.0033 | 0.0001 | 0.0001 | 0.0001 | 0.0027 | 0.0007 | 0.0024 | 0.0004 | 0.0153 | 0.0002 | 0.003 | 0.0001 | 0.0155 | 0.0062 | 0.0237 | 0.0026 |
None | 0.7156 | 0.7793 | 0.7636 | 0.7521 | 0.5511 | 0.7251 | 0.765 | 0.6801 | 0.7779 | 0.7521 | 0.6245 | 0.8363 | 0.7053 | 0.7487 | 0.7375 | 0.5685 | 0.3078 | 0.7544 |
Nuclear Periphery | 0.052 | 0.0001 | 0.0004 | 0.0003 | 0.0003 | 0.0004 | 0.0078 | 0.0004 | 0.0061 | 0.0005 | 0.0007 | 0.0004 | 0.0004 | 0.0002 | 0.0008 | 0.0004 | 0.0005 | 0.0002 |
Nucleolus | 0.0005 | 0 | 0.0001 | 0 | 0 | 0.0001 | 0.0006 | 0.0001 | 0.0006 | 0.0001 | 0.0003 | 0.0001 | 0.0003 | 0.0001 | 0.0002 | 0.0004 | 0.0007 | 0.0007 |
Nucleus | 0.0033 | 0.0002 | 0.0061 | 0.0007 | 0.0006 | 0.0004 | 0.0023 | 0.0007 | 0.0011 | 0.0007 | 0.0009 | 0.0007 | 0.0005 | 0.0007 | 0.0007 | 0.0006 | 0.0007 | 0.0005 |
Peroxisomes | 0.0065 | 0 | 0.0261 | 0.0001 | 0 | 0.0008 | 0.0138 | 0.0003 | 0.0166 | 0.0034 | 0.023 | 0.0002 | 0.0124 | 0.0002 | 0.0099 | 0.007 | 0.14 | 0.0008 |
Punctate Nuclear | 0.0011 | 0.0001 | 0.0002 | 0.0002 | 0.0002 | 0.0007 | 0.0032 | 0.0004 | 0.0014 | 0.0005 | 0.0009 | 0.0016 | 0.0014 | 0.0003 | 0.0012 | 0.0007 | 0.0044 | 0.0011 |
Vacuole | 0.0048 | 0 | 0.0005 | 0.0003 | 0.0003 | 0.0005 | 0.0015 | 0.0004 | 0.0007 | 0.0023 | 0.0028 | 0.0003 | 0.0012 | 0.0002 | 0.0008 | 0.0017 | 0.0017 | 0.0001 |
Vacuole Periphery | 0.0047 | 0 | 0.0001 | 0 | 0 | 0.0001 | 0.0011 | 0.0001 | 0.0003 | 0.0004 | 0.0009 | 0.0001 | 0.0003 | 0 | 0.0002 | 0.0003 | 0.0008 | 0.0001 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 9.0681 | 11.0246 | 10.1056 | 8.9616 | 6.584 | 10.8757 | 8.6881 | 9.0724 | 12.9366 | 11.1463 |
Translational Efficiency | 0.6896 | 0.4821 | 0.4746 | 0.6075 | 0.8358 | 0.6438 | 0.6961 | 0.7763 | 0.514 | 0.63 |
Dataset | Hit |
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Protein Concentration | ✔ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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