Standard name
Human Ortholog
Description Putative metal transporter involved in mitochondrial iron accumulation; MMT1 has a paralog, MMT2, that arose from the whole genome duplication
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0
Bud 0 0 0.05 0 0 0 0 0 0.05 0 0 0 0 0 0 0.09 0.05 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.09 0.06 0.08 0 0 0 0 0 0
Cytoplasm 0.09 0.25 0.16 0.16 0.16 0.08 0.06 0.08 0.07 0 0 0 0.46 0.33 0.38 0.22 0.3 0.25 0 0.06 0 0.05 0.07 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0.05 0.06 0 0 0 0 0 0 0
Endosome 0.1 0 0.07 0 0.08 0 0 0 0 0 0 0 0.05 0.09 0.1 0.16 0.12 0.11 0.09 0.07 0.08 0.08 0.09 0.07
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0.09 0.18 0.05 0.07 0.16 0 0 0 0 0 0
Mitochondria 0.74 0.69 0.65 0.79 0.7 0.9 0.93 0.88 0.94 0.95 0.94 0.98 0.25 0.37 0.29 0.14 0.17 0.1 0.67 0.6 0.66 0.61 0.57 0.59
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.07 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0.06 0 0.08 0 0 0 0 0 0 0 0 0 0 0.05 0 0.16 0.12 0.07 0.05 0 0.07 0.06 0.06 0
SpindlePole 0 0 0.1 0 0.05 0 0 0.05 0 0 0 0 0 0 0 0.16 0.08 0.19 0 0 0 0 0 0
Vac/Vac Membrane 0.18 0.13 0 0 0.12 0 0.05 0 0 0 0 0 0.12 0.15 0.14 0.11 0.1 0.11 0 0.06 0 0.06 0 0.06
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 6 1 0 0 0 0 0 0 0 1 1 9 9 6 1 1 0 12 20 12 7 23 22
Bud 5 9 16 13 4 21 10 23 23 15 11 15 5 2 2 7 6 4 7 12 1 5 13 13
Bud Neck 1 0 4 0 0 1 0 0 0 0 0 0 2 1 1 0 1 0 2 4 3 5 5 13
Bud Site 0 0 0 1 0 10 3 9 10 11 11 9 1 0 0 3 0 0 – – – – – –
Cell Periphery 10 10 5 5 3 1 1 4 3 0 4 1 3 9 8 7 7 8 0 1 1 0 1 3
Cytoplasm 41 90 48 52 48 40 34 58 30 23 19 17 103 81 79 18 36 27 9 23 11 18 39 24
Endoplasmic Reticulum 7 8 3 4 1 2 4 3 2 2 3 1 13 7 2 4 7 4 1 3 2 2 4 4
Endosome 44 10 22 11 25 2 3 6 1 1 0 0 12 22 20 13 15 12 44 29 30 32 47 38
Golgi 9 2 4 2 1 0 0 1 0 0 0 0 13 23 37 4 9 17 20 12 10 4 10 17
Mitochondria 323 251 199 264 214 429 492 615 423 488 440 538 56 91 60 11 20 11 338 243 245 235 309 332
Nucleus 3 2 2 0 4 7 7 11 9 8 3 5 0 3 1 3 6 7 1 1 0 1 0 0
Nuclear Periphery 10 5 0 8 4 9 20 11 23 18 19 23 0 0 0 0 2 2 5 2 1 5 2 4
Nucleolus 5 1 1 4 3 15 22 34 11 15 10 20 0 0 0 1 1 0 2 0 0 1 3 2
Peroxisomes 26 10 23 8 13 4 3 14 3 5 2 2 2 13 7 13 15 7 24 8 26 21 32 21
SpindlePole 14 4 32 14 15 19 11 36 13 15 15 13 9 8 5 13 10 20 10 7 7 11 16 21
Vac/Vac Membrane 79 47 13 13 36 16 27 29 18 19 17 15 28 37 29 9 12 12 16 25 5 21 22 31
Unique Cell Count 438 365 305 333 306 478 528 699 452 516 470 550 224 243 210 81 120 106 503 406 370 383 547 559
Labelled Cell Count 577 455 373 399 371 576 637 854 569 620 555 660 256 306 257 107 148 131 503 406 370 383 547 559


Mitochondria

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.9 5.1 4.7 4.3 4.4 3.6 3.5 4.0 3.8 3.5 3.7 3.8 5.9 6.1 6.8 5.3 5.9 6.8 5.7 6.1 6.5
Std Deviation (1e-4) 1.5 1.2 1.2 1.1 1.7 1.3 1.4 1.2 1.3 1.1 1.1 1.1 1.2 1.3 1.5 1.5 1.8 2.1 1.4 1.7 2.0
Intensity Change (Log2) – – – -0.12 -0.08 -0.4 -0.41 -0.23 -0.31 -0.41 -0.33 -0.29 0.33 0.39 0.54 0.19 0.34 0.54 0.29 0.39 0.46


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 -1.8 -2.9 -2.6 0 -0.1 -0.6
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 1.5 1.5 0 0 0
Cytoplasm 0 0 -3.2 -4.3 -3.5 -4.0 -5.6 -5.7 -6.7 7.6 4.8 5.7 1.4 3.3 2.2
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 3.2 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 -0.9 0.8 0.9 2.5 1.7 1.3
Golgi 0 0 0 0 0 0 0 0 0 2.9 4.4 6.7 0 0 5.9
Mitochondria 0 0 0 0 0 0 0 0 0 -9.2 -6.5 -8.2 -8.3 -9.0 -9.7
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 -3.6 -1.0 -2.0 2.3 1.6 -0.3
SpindlePole 0 0 0 0 0 0 0 0 0 -2.8 -3.2 -3.5 1.4 -0.7 2.2
Vacuole -0.2 3.4 -0.7 0.6 -0.1 -0.2 -0.4 -0.5 -1.2 3.5 4.4 3.9 0 2.3 2.6

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 1.1038 1.8394 1.5278 1.3111 0.8894 1.6393 3.8542 3.5989 3.8325 3.9027 3.5139 4.0182 3.7785 3.9938 4.5222 4.0834 3.9782 3.6908
Actin 0.0131 0.0165 0.0047 0.0129 0.0037 0.0041 0.0554 0.0108 0.016 0.0239 0.0151 0.0162 0.045 0.0197 0.0252 0.0521 0.0198 0.0111
Bud 0.0009 0.0106 0.0026 0.0052 0.0062 0.0022 0.0009 0.002 0.003 0.0015 0.0006 0.0015 0.002 0.0055 0.0034 0.0077 0.0007 0.0017
Bud Neck 0.0007 0.0014 0.0018 0.0066 0.001 0.0024 0.0013 0.0014 0.0033 0.0015 0.0005 0.0019 0.0065 0.0018 0.0016 0.0109 0.0012 0.0027
Bud Periphery 0.0024 0.0175 0.0059 0.0181 0.0331 0.0058 0.0026 0.0048 0.0094 0.0032 0.0022 0.0057 0.0052 0.0126 0.0092 0.0332 0.0021 0.0097
Bud Site 0.0047 0.0268 0.0127 0.0023 0.0014 0.0016 0.0025 0.0325 0.0058 0.0033 0.0005 0.0019 0.0234 0.0533 0.0064 0.0143 0.0029 0.0007
Cell Periphery 0.0034 0.0014 0.0008 0.0013 0.0008 0.0006 0.0011 0.0009 0.0016 0.0005 0.0002 0.0007 0.0033 0.0022 0.0017 0.0055 0.0014 0.0006
Cytoplasm 0.014 0.019 0.0293 0.0697 0.0197 0.0169 0.0127 0.013 0.0121 0.0025 0.0013 0.0109 0.0153 0.0314 0.0207 0.0247 0.024 0.0132
Cytoplasmic Foci 0.0528 0.062 0.0691 0.0311 0.0273 0.0752 0.0403 0.0541 0.0403 0.0429 0.025 0.0493 0.0596 0.0738 0.0473 0.0852 0.0484 0.0651
Eisosomes 0.0009 0.0006 0.0003 0.0003 0.0002 0.0003 0.0026 0.0008 0.001 0.0005 0.0004 0.0006 0.0011 0.0012 0.0012 0.0013 0.0021 0.0008
Endoplasmic Reticulum 0.0036 0.0023 0.0011 0.0041 0.0016 0.0018 0.0018 0.0014 0.0008 0.0003 0.0002 0.0012 0.0073 0.0026 0.0044 0.0007 0.0033 0.0023
Endosome 0.1301 0.0875 0.0663 0.0579 0.0757 0.0803 0.0712 0.0707 0.0347 0.0504 0.0712 0.0464 0.1017 0.0823 0.0866 0.074 0.0975 0.0626
Golgi 0.0498 0.0522 0.0314 0.0338 0.0349 0.0392 0.0427 0.0477 0.0322 0.0573 0.0249 0.0307 0.078 0.066 0.0344 0.0954 0.0418 0.0681
Lipid Particles 0.0375 0.0114 0.0101 0.0039 0.0137 0.0288 0.0476 0.0242 0.01 0.0161 0.0199 0.0195 0.0349 0.0178 0.0159 0.0055 0.0247 0.0217
Mitochondria 0.5751 0.5764 0.6522 0.6799 0.6724 0.6344 0.612 0.5988 0.716 0.7535 0.6651 0.6959 0.5002 0.5199 0.5772 0.4913 0.6091 0.6386
None 0.0489 0.0511 0.0574 0.0475 0.0538 0.0319 0.048 0.0381 0.0572 0.0009 0.0905 0.033 0.0579 0.0444 0.0784 0.0225 0.0381 0.0498
Nuclear Periphery 0.0023 0.0011 0.0005 0.0008 0.0008 0.0011 0.0017 0.0008 0.0004 0.0001 0.0001 0.0004 0.0035 0.0015 0.0027 0.0002 0.0043 0.0003
Nucleolus 0.0008 0.0034 0.0015 0.0006 0.0017 0.0013 0.0005 0.0011 0.0007 0.0001 0.0004 0.0003 0.0009 0.0008 0.0008 0.0004 0.0006 0.0003
Nucleus 0.0012 0.003 0.001 0.0014 0.0039 0.0008 0.0006 0.0006 0.0006 0.0002 0.0002 0.0003 0.0015 0.0012 0.0014 0.0004 0.0011 0.0003
Peroxisomes 0.0384 0.0365 0.0395 0.0084 0.0287 0.0588 0.0468 0.0833 0.0467 0.0374 0.0764 0.077 0.038 0.0505 0.0516 0.0697 0.0685 0.0438
Punctate Nuclear 0.0012 0.005 0.0017 0.0003 0.0052 0.0025 0.0011 0.0004 0.0008 0.0002 0.0004 0.0018 0.0015 0.0005 0.0017 0.0006 0.0024 0.0008
Vacuole 0.0126 0.01 0.0075 0.0095 0.0087 0.006 0.0037 0.0071 0.0051 0.0021 0.0024 0.0025 0.0071 0.0087 0.0133 0.0027 0.0035 0.0031
Vacuole Periphery 0.0054 0.0045 0.0026 0.0044 0.0055 0.0039 0.003 0.0055 0.0023 0.0017 0.0026 0.0022 0.0061 0.0026 0.0149 0.0016 0.0025 0.0026

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 13.3157 8.3646 7.6392 12.254 16.3312 10.0712 10.4091 9.0014 11.5747 16.0719
Translational Efficiency 1.0553 1.1716 0.9751 0.6068 0.7534 1.2676 0.7685 0.7565 0.6227 0.6478

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✔
Translational Efficiency ✔
Standard name
Human Ortholog
Description Putative metal transporter involved in mitochondrial iron accumulation; MMT1 has a paralog, MMT2, that arose from the whole genome duplication
Localization
Cell Percentages mitochondrion (90%)
Cell Cycle Regulation No
Subcompartmental Group mito-2

Mmt1

Mmt1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Mmt1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available