Standard name
Human Ortholog
Description Cytoplasmic chaperone of the Hsp90 family; plays a role in determining prion variants; redundant in function and nearly identical with Hsp82p, and together they are essential; expressed constitutively at 10-fold higher basal levels than HSP82 and induced 2-3 fold by heat shock; contains two acid-rich unstructured regions that promote the solubility of chaperone-substrate complexes; HSC82 has a paralog, HSP82, that arose from the whole genome duplication
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.31 0.28 0.15 0 0 0 0 0 0
Cytoplasm 0.99 0.99 1.0 0.99 0.99 0.98 1.0 0.94 0.93 0.95 0.87 0.93 1.0 0.98 1.0 0.84 0.83 0.91 0.98 0.96 0.97 0.89 0.92 0.89
Endoplasmic Reticulum 0 0 0 0 0 0 0 0.05 0 0 0.1 0 0 0 0 0 0.05 0.1 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 1 0 0 0 2 3 3 1 0 2 0 0 0 0 0 0 0 0 0 1
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 4 3 2 2 3 7 1 5 6 5 7 7 0 1 2 89 71 34 0 0 1 0 0 0
Cytoplasm 233 126 181 148 164 220 211 179 202 238 241 194 160 188 209 241 207 203 244 141 181 74 171 154
Endoplasmic Reticulum 0 0 0 0 0 1 1 9 9 8 28 8 0 1 0 8 13 22 0 0 0 2 4 7
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 2 2 0 2 0 1 2 1
Unique Cell Count 235 127 181 149 165 224 212 191 218 251 278 208 160 191 210 287 250 222 251 147 186 84 186 173
Labelled Cell Count 237 129 183 150 168 228 213 193 221 254 283 210 160 192 211 338 293 261 251 147 186 84 186 173


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 340.7 337.5 292.3 254.4 249.3 229.8 236.7 218.8 211.9 203.2 188.9 220.9 321.5 354.5 369.7 523.8 500.8 439.2 286.4 318.1 349.3
Std Deviation (1e-4) 70.7 75.2 63.0 68.1 54.9 56.9 54.7 52.4 63.4 61.1 57.6 72.1 72.9 85.5 97.2 138.8 151.5 146.5 70.2 72.0 99.6
Intensity Change (Log2) – – – -0.2 -0.23 -0.35 -0.3 -0.42 -0.46 -0.52 -0.63 -0.4 0.14 0.28 0.34 0.84 0.78 0.59 -0.03 0.12 0.26


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 8.0 7.5 5.0
Cytoplasm -1.1 -1.0 -1.8 -0.9 -3.4 -3.7 -3.1 -5.1 -3.6 0 -1.7 -0.9 -5.7 -5.9 -4.0
Endoplasmic Reticulum 0 0 0 0 0 0 0 4.4 0 0 0 0 0 3.1 4.4
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 247.7904 365.8219 331.0484 279.12 323.892 331.2065 324.4983 456.684 383.6641 352.3952 324.964 374.2412 282.853 399.1479 342.4086 285.4149 326.2491 423.9381
Actin 0.0033 0.0001 0.0002 0.0001 0.0004 0.0002 0.0028 0.0001 0.0007 0.0012 0.0066 0.0002 0.0019 0.0002 0.0008 0.0008 0.0004 0.0002
Bud 0.0021 0.0001 0.0012 0.0002 0.0003 0.0002 0.0011 0.0001 0.001 0.0002 0.0034 0.0006 0.0008 0.0003 0.0009 0.0004 0.0011 0.0001
Bud Neck 0.0005 0.0003 0.0004 0.0005 0.0008 0.0011 0.0002 0.0001 0.0003 0.0004 0.0007 0.0004 0.0002 0.0001 0.0003 0.0002 0.0005 0.0003
Bud Periphery 0.001 0 0.0004 0 0.0001 0.0001 0.0009 0 0.0003 0.0001 0.0059 0.0001 0.0004 0.0001 0.0004 0.0003 0.0003 0
Bud Site 0.001 0.0002 0.0001 0.0001 0 0.0001 0.0005 0 0.0002 0.0001 0.0007 0.0001 0.0006 0.0001 0.0014 0.0001 0.0009 0
Cell Periphery 0.004 0.0026 0.0007 0.0008 0.0003 0.0011 0.0016 0.0001 0.0005 0.0012 0.0014 0.0017 0.0012 0.0001 0.0013 0.001 0.0014 0
Cytoplasm 0.7963 0.9757 0.9554 0.8252 0.927 0.9254 0.8449 0.9938 0.9339 0.9134 0.7783 0.9727 0.8854 0.9835 0.9577 0.8623 0.898 0.9877
Cytoplasmic Foci 0.0127 0.0001 0.0077 0.0457 0.0097 0.0004 0.0083 0.0001 0.0088 0.0087 0.0043 0.0023 0.002 0.0002 0.0022 0.0003 0.0143 0.0002
Eisosomes 0.001 0.0003 0.0002 0.0007 0.0003 0.0001 0.0008 0 0.0003 0.0003 0.0002 0 0.0003 0 0.0004 0.0001 0.0001 0
Endoplasmic Reticulum 0.0015 0.0004 0.0002 0.0002 0.0003 0.0003 0.0035 0.0001 0.0009 0.0002 0.0122 0.0012 0.0017 0.0001 0.0004 0.0002 0.0003 0.0001
Endosome 0.0035 0 0.0001 0.0002 0.0001 0.0002 0.0036 0 0.0049 0.0002 0.0233 0.0019 0.0009 0 0.0004 0.0006 0.0019 0
Golgi 0.0008 0 0 0.0001 0 0 0.0005 0 0.0003 0.0004 0.0022 0.0001 0.0002 0 0.0001 0.0001 0.0003 0
Lipid Particles 0.0027 0.0001 0.0014 0.0079 0.0015 0.0001 0.0022 0 0.001 0.0015 0.0014 0.0002 0.0007 0 0.0006 0.0001 0.0017 0
Mitochondria 0.0182 0.0005 0.0039 0.0224 0.0027 0.0028 0.017 0.0002 0.0067 0.037 0.0409 0.0001 0.005 0.0015 0.0048 0.0257 0.0119 0.0003
None 0.0142 0.0011 0.0033 0.0051 0.0179 0.0009 0.0099 0.0003 0.0121 0.0093 0.0224 0.0006 0.003 0.0013 0.0047 0.0008 0.0059 0.0003
Nuclear Periphery 0.0276 0.0035 0.0033 0.0125 0.0052 0.0049 0.0336 0.001 0.0083 0.0038 0.0292 0.0034 0.0442 0.0006 0.0026 0.0432 0.002 0.0019
Nucleolus 0.0193 0.0025 0.0093 0.0444 0.0099 0.0156 0.0171 0.0001 0.0042 0.0069 0.016 0.0002 0.0064 0.005 0.0047 0.0041 0.0116 0
Nucleus 0.0662 0.0093 0.0089 0.0229 0.0199 0.0273 0.0375 0.0037 0.0096 0.0105 0.0223 0.0044 0.0313 0.006 0.012 0.0308 0.0099 0.0085
Peroxisomes 0.0056 0 0.0003 0.0023 0.0002 0.0001 0.0008 0 0.0003 0.0002 0.0003 0 0.0002 0 0.0002 0.0001 0.0009 0
Punctate Nuclear 0.0017 0.0001 0.0004 0.0006 0.001 0.0005 0.0039 0 0.0012 0.0004 0.0011 0.0003 0.0005 0.0001 0.0006 0.0001 0.0003 0.0001
Vacuole 0.011 0.0025 0.0016 0.0043 0.0011 0.0117 0.0041 0.0002 0.0023 0.0025 0.0124 0.0077 0.0047 0.0006 0.0022 0.0046 0.0228 0.0001
Vacuole Periphery 0.0056 0.0003 0.0009 0.0037 0.0011 0.0068 0.0052 0.0001 0.0023 0.0016 0.0147 0.0018 0.0084 0.0002 0.0013 0.024 0.0136 0.0002

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 1457.6608 1901.4985 2317.0988 2096.006 1879.1137 1531.3107 1941.6739 2077.539 2467.6115 1782.5861
Translational Efficiency 1.6911 1.6312 1.4993 1.8437 1.6451 1.3479 1.4698 1.6549 1.3589 1.6221

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Cytoplasmic chaperone of the Hsp90 family; plays a role in determining prion variants; redundant in function and nearly identical with Hsp82p, and together they are essential; expressed constitutively at 10-fold higher basal levels than HSP82 and induced 2-3 fold by heat shock; contains two acid-rich unstructured regions that promote the solubility of chaperone-substrate complexes; HSC82 has a paralog, HSP82, that arose from the whole genome duplication
Localization
Cell Percentages cytoplasm (100%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-1

Hsc82

Hsc82


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Hsc82-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available