Standard name
Human Ortholog
Description Ribosomal 60S subunit protein L36A; N-terminally acetylated; binds to 5.8 S rRNA; homologous to mammalian ribosomal protein L36, no bacterial homolog; RPL36A has a paralog, RPL36B, that arose from the whole genome duplication
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0.06 0.07 0.06 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0.05 0 0 0.09 0.06 0.07 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.98 0.98 0.99 0.97 0.9 0.89 0.8 0.78 0.77 0.68 0.74 0.67 0.99 0.99 0.99 0.95 0.96 0.91 0.97 0.96 0.92 0.96 0.91 0.93
Endoplasmic Reticulum 0 0 0 0 0.06 0.09 0.14 0.15 0.14 0.2 0.13 0.11 0 0 0 0.05 0.05 0 0 0 0 0 0.05 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0.08 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0.06 0.06 0 0.11 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0.08 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 2 6 5 8 13 13 10 0 0 0 0 1 0 0 0 0 0 0 0
Bud 1 0 0 0 2 0 0 0 0 0 0 0 0 0 0 0 0 0 1 6 1 0 0 1
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 2 8 3 2 4 8 11 9 10 18 11 11 1 4 8 4 1 2 0 0 0 0 0 1
Cytoplasm 438 735 150 114 121 207 163 189 184 142 132 112 223 381 390 399 268 259 421 717 137 172 257 240
Endoplasmic Reticulum 3 17 1 3 8 22 28 36 34 42 24 18 5 5 9 19 13 7 2 5 4 0 14 6
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0
Golgi 3 0 0 0 0 1 4 4 4 8 6 13 1 1 0 8 1 8 0 0 0 0 1 1
Mitochondria 1 0 0 0 1 0 2 3 15 12 8 19 1 0 0 8 2 1 0 1 0 0 0 1
Nucleus 0 0 1 0 0 0 0 2 0 1 0 0 0 0 0 0 1 1 1 1 1 0 0 1
Nuclear Periphery 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 1 2 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 2 1 3 8 0 3 8 10 0 1 0 9 2 22 2 8 2 3 3 1
Unique Cell Count 445 749 151 117 134 233 204 243 238 210 178 168 226 386 395 419 280 285 435 746 150 179 282 259
Labelled Cell Count 448 760 155 119 139 241 217 256 255 239 202 194 231 393 407 448 291 300 435 746 150 179 282 259


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 72.5 57.7 55.9 49.9 31.1 29.7 26.0 23.5 21.1 21.3 19.7 18.0 57.9 53.8 50.7 63.8 83.5 68.8 60.5 62.7 66.3
Std Deviation (1e-4) 13.8 12.7 12.9 8.4 9.2 6.7 4.8 5.1 4.7 4.2 4.5 4.5 13.0 11.5 9.9 21.0 25.9 24.2 14.9 17.2 17.9
Intensity Change (Log2) – – – -0.17 -0.85 -0.91 -1.11 -1.25 -1.41 -1.39 -1.51 -1.64 0.05 -0.06 -0.14 0.19 0.58 0.3 0.11 0.16 0.25


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 3.1 3.4 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 1.6 0 1.2 2.6 1.9 2.0 0 0 0 0 0 0
Cytoplasm -1.3 -3.5 -3.9 -5.6 -6.0 -6.1 -7.6 -6.5 -7.6 -0.6 -0.6 -0.6 -2.3 -2.1 -3.5
Endoplasmic Reticulum 0 0 3.5 4.4 4.7 4.6 5.6 4.4 3.8 0 0 0 2.2 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 3.5 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 3.1 3.0 0 4.3 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 3.5

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 54.1103 62.235 56.9682 58.0968 61.7711 54.4887 63.7697 82.0348 75.346 67.6516 61.7306 73.5078 57.8142 67.2855 64.3313 61.0894 53.0449 62.5529
Actin 0.0043 0.0003 0.0017 0.0005 0.0006 0.0026 0.0054 0.0028 0.0025 0.0005 0.0017 0.0014 0.0008 0.0003 0.0004 0.0006 0.0263 0.0009
Bud 0.0009 0.0003 0.001 0.0013 0.0002 0.002 0.0012 0.0003 0.0021 0.0011 0.0006 0.0007 0.0004 0.0003 0.0004 0.0007 0.0007 0.0003
Bud Neck 0.0005 0.0005 0.0008 0.0007 0.0018 0.0018 0.0015 0.0004 0.0006 0.0005 0.0004 0.0014 0.0006 0.0004 0.0006 0.0012 0.0008 0.0015
Bud Periphery 0.0009 0.0002 0.0007 0.0008 0.0001 0.0019 0.0018 0.0001 0.0015 0.0009 0.0005 0.0006 0.0004 0.0002 0.0004 0.0007 0.0005 0.0002
Bud Site 0.0006 0.0002 0.0005 0.0002 0 0.0007 0.0036 0.0002 0.0005 0.0003 0.0003 0.0001 0.0002 0.0003 0.0003 0.0001 0.0003 0.0001
Cell Periphery 0.0047 0.0032 0.0019 0.0013 0.0004 0.0039 0.0042 0.0021 0.0017 0.0009 0.0018 0.0007 0.0054 0.0066 0.003 0.0122 0.0009 0.0008
Cytoplasm 0.7779 0.9524 0.8705 0.8307 0.8084 0.75 0.7878 0.9589 0.8918 0.8049 0.7292 0.8567 0.8279 0.9409 0.8992 0.7987 0.7292 0.8518
Cytoplasmic Foci 0.0026 0.0012 0.0061 0.0027 0.0075 0.0114 0.0081 0.0013 0.0044 0.0033 0.0056 0.0061 0.0031 0.0012 0.0026 0.0037 0.0286 0.0057
Eisosomes 0.0001 0 0.0001 0 0 0.0001 0.0002 0.0003 0.0001 0.0001 0.0002 0.0001 0 0 0.0001 0 0 0
Endoplasmic Reticulum 0.0726 0.0146 0.0261 0.0224 0.0948 0.0666 0.0721 0.0133 0.0214 0.0077 0.0487 0.0688 0.0696 0.0171 0.0366 0.0118 0.0534 0.0863
Endosome 0.0121 0.0025 0.0097 0.0126 0.0296 0.0298 0.0116 0.0019 0.0113 0.0158 0.0226 0.0186 0.0102 0.002 0.0076 0.0275 0.0302 0.0168
Golgi 0.0027 0.0005 0.0026 0.0011 0.0035 0.0158 0.0018 0.0009 0.0029 0.0006 0.0018 0.0101 0.0012 0.0005 0.001 0.0019 0.0031 0.004
Lipid Particles 0.0009 0.0004 0.0023 0.0002 0.0003 0.0018 0.0044 0.0001 0.0007 0.0007 0.0023 0.0007 0.0026 0.0003 0.0009 0.0008 0.0095 0.0004
Mitochondria 0.0074 0.0004 0.0067 0.004 0.0009 0.0136 0.006 0.0028 0.0112 0.0077 0.0296 0.0065 0.0022 0.0003 0.0046 0.0002 0.0022 0.0022
None 0.0016 0.0001 0.0009 0.0004 0.0002 0.0129 0.005 0.0002 0.0005 0.0022 0.0085 0.0009 0.0008 0.0001 0.0009 0.0002 0.0027 0.0012
Nuclear Periphery 0.0248 0.0019 0.0081 0.0057 0.006 0.0103 0.0157 0.003 0.0027 0.007 0.0203 0.0051 0.0132 0.0018 0.004 0.0045 0.008 0.0072
Nucleolus 0.0003 0 0.0005 0.0001 0 0.0012 0.001 0.0001 0.0001 0.0004 0.0018 0.0001 0.0005 0.0001 0.0006 0.0001 0.0006 0.0012
Nucleus 0.0049 0.001 0.0021 0.0025 0.0016 0.008 0.0055 0.0012 0.0015 0.0037 0.0061 0.0014 0.0037 0.0019 0.0015 0.0016 0.0023 0.003
Peroxisomes 0.0002 0 0.0024 0 0.0001 0.0002 0.0011 0 0.003 0.0002 0.0003 0.0001 0.0008 0 0.0001 0 0.0024 0
Punctate Nuclear 0.0013 0 0.0003 0 0.0001 0.0006 0.0035 0 0.0002 0.0001 0.0005 0.0001 0.0005 0 0.0001 0 0.0019 0.0005
Vacuole 0.0612 0.0166 0.0447 0.0976 0.0305 0.0408 0.0467 0.009 0.0325 0.113 0.0832 0.0141 0.047 0.0246 0.0311 0.1121 0.076 0.0127
Vacuole Periphery 0.0172 0.0037 0.0106 0.0151 0.0134 0.024 0.0117 0.001 0.0067 0.0282 0.0339 0.0057 0.0087 0.0011 0.0042 0.0214 0.0203 0.0034

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 764.7358 992.8869 1183.6551 937.002 1010.3993 928.0936 850.0294 820.8588 884.2144 759.1274
Translational Efficiency 3.0037 2.656 2.2533 3.1344 2.9564 2.4177 2.602 4.175 2.666 2.9913

Hit Data

Dataset Hit
Protein Concentration ✔
Protein Localization ✔
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Ribosomal 60S subunit protein L36A; N-terminally acetylated; binds to 5.8 S rRNA; homologous to mammalian ribosomal protein L36, no bacterial homolog; RPL36A has a paralog, RPL36B, that arose from the whole genome duplication
Localization
Cell Percentages cytoplasm (96%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-2

Rpl36a

Rpl36a


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Rpl36a-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available