Standard name | |
---|---|
Human Ortholog | |
Description | Protein that recognizes and binds damaged DNA during NER; subunit of Nucleotide Excision Repair Factor 1 (NEF1); contains zinc finger motif; homolog of human XPA protein; NER stands for nucleotide excision repair |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 |
Bud | 0 | 0 | 1 | 0 | 3 | 1 | 13 | 7 | 10 | 14 | 9 | 9 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 1 | 2 | 5 | 5 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 4 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 2 | 9 | 9 | 4 | 24 | 9 | 8 | 0 | 0 | 0 | 0 | 1 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 3 | 4 | 37 | 39 | 41 | 49 | 45 | 59 | 36 | 43 | 24 | 35 | 91 | 90 | 177 | 34 | 19 | 12 | 5 | 4 | 10 | 16 | 77 | 65 |
Endoplasmic Reticulum | 1 | 0 | 3 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3 | 1 | 3 | 12 | 5 | 4 | 0 | 0 | 0 | 0 | 0 | 1 |
Endosome | 0 | 0 | 1 | 3 | 7 | 3 | 0 | 1 | 2 | 1 | 0 | 0 | 3 | 4 | 2 | 12 | 4 | 7 | 0 | 1 | 3 | 0 | 2 | 5 |
Golgi | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 1 | 0 | 0 | 0 | 1 | 0 | 1 | 0 |
Mitochondria | 39 | 12 | 24 | 91 | 71 | 196 | 367 | 342 | 255 | 408 | 233 | 230 | 2 | 3 | 2 | 17 | 11 | 5 | 1 | 1 | 8 | 0 | 11 | 13 |
Nucleus | 43 | 32 | 113 | 82 | 92 | 141 | 262 | 288 | 210 | 273 | 147 | 158 | 209 | 197 | 277 | 144 | 79 | 48 | 60 | 51 | 115 | 48 | 135 | 107 |
Nuclear Periphery | 0 | 0 | 0 | 1 | 0 | 3 | 0 | 6 | 0 | 2 | 3 | 0 | 0 | 0 | 0 | 3 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 1 | 1 | 0 | 1 | 3 | 4 | 3 | 3 | 3 | 4 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 3 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 3 | 2 | 2 | 0 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 5 | 2 | 15 | 17 |
Vac/Vac Membrane | 11 | 7 | 19 | 33 | 38 | 31 | 27 | 25 | 24 | 28 | 10 | 17 | 25 | 49 | 67 | 57 | 49 | 29 | 20 | 13 | 59 | 11 | 111 | 103 |
Unique Cell Count | 74 | 42 | 177 | 197 | 204 | 309 | 514 | 525 | 386 | 540 | 293 | 320 | 315 | 324 | 491 | 243 | 143 | 87 | 94 | 74 | 213 | 85 | 370 | 335 |
Labelled Cell Count | 97 | 55 | 200 | 252 | 253 | 427 | 726 | 744 | 546 | 799 | 438 | 463 | 336 | 345 | 528 | 283 | 170 | 105 | 94 | 74 | 213 | 85 | 370 | 335 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 3.6 | 4.3 | 4.3 | 4.0 | 4.5 | 3.7 | 3.4 | 3.4 | 3.5 | 3.2 | 3.2 | 3.2 | 5.5 | 5.4 | 5.3 | 6.8 | 6.6 | 6.4 | 5.4 | 5.6 | 6.0 |
Std Deviation (1e-4) | 0.5 | 0.7 | 1.0 | 1.5 | 2.5 | 1.8 | 1.2 | 1.1 | 1.9 | 1.3 | 1.7 | 1.8 | 0.9 | 1.0 | 0.9 | 1.8 | 1.4 | 1.1 | 1.3 | 1.3 | 1.7 |
Intensity Change (Log2) | – | – | – | -0.1 | 0.06 | -0.24 | -0.36 | -0.35 | -0.29 | -0.42 | -0.46 | -0.44 | 0.33 | 0.33 | 0.3 | 0.64 | 0.6 | 0.57 | 0.33 | 0.37 | 0.47 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Actin | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Bud Neck | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Bud Site | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Cell Periphery | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Cyto | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endoplasmic Reticulum | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Endosome | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Golgi | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Mitochondria | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Nuclear Periphery | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Nuc | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleolus | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Peroxisomes | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
SpindlePole | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Vac | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cortical Patches | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Cytoplasm | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Nucleus | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Vacuole | – | – | – | – | – | – | – | – | – | – | – | – | 3.3 | 5.1 | 4.5 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 1.2236 | 0.5983 | 0.777 | 1.133 | 0.9095 | 0.4754 | -0.6306 | -0.6999 | -0.5338 | -0.8111 | -0.8758 | -0.8271 | -1.7311 | -1.0841 | -1.2377 | -1.2384 | -1.5131 | -1.3747 |
Actin | 0.0521 | 0.0003 | 0.0315 | 0.0005 | 0.0106 | 0.0041 | 0.0115 | 0.0001 | 0.0034 | 0.0199 | 0 | 0.0016 | 0.0169 | 0.0002 | 0.0154 | 0.0487 | 0.0153 | 0.0014 |
Bud | 0.0005 | 0.0001 | 0.0008 | 0.0005 | 0.0008 | 0.0001 | 0.001 | 0.0001 | 0.0016 | 0.0009 | 0 | 0.0007 | 0.0001 | 0.0001 | 0.001 | 0.0002 | 0.0002 | 0.0003 |
Bud Neck | 0.0042 | 0.0005 | 0.0012 | 0.0059 | 0.0016 | 0.0021 | 0.0153 | 0.0001 | 0.0005 | 0.0016 | 0.0003 | 0.0026 | 0.0006 | 0.006 | 0.0015 | 0.0014 | 0.0013 | 0.0038 |
Bud Periphery | 0.0007 | 0.0001 | 0.0015 | 0.0004 | 0.001 | 0.0001 | 0.0014 | 0.0001 | 0.0017 | 0.0008 | 0 | 0.0004 | 0.0001 | 0 | 0.0017 | 0.0002 | 0.0003 | 0.0003 |
Bud Site | 0.0073 | 0.001 | 0.0113 | 0.0045 | 0.0035 | 0.0002 | 0.0157 | 0.0005 | 0.0084 | 0.003 | 0.0001 | 0.0005 | 0.001 | 0.005 | 0.0051 | 0.0041 | 0.0007 | 0.0008 |
Cell Periphery | 0.0002 | 0.0001 | 0.0005 | 0.0001 | 0.0001 | 0 | 0.0004 | 0.0001 | 0.0002 | 0.0001 | 0 | 0.0001 | 0.0001 | 0 | 0.0002 | 0.0001 | 0.0001 | 0 |
Cytoplasm | 0.1065 | 0.1752 | 0.1016 | 0.1709 | 0.0981 | 0.109 | 0.1348 | 0.2124 | 0.1531 | 0.1087 | 0.2477 | 0.3714 | 0.1408 | 0.1376 | 0.1345 | 0.0986 | 0.1185 | 0.0906 |
Cytoplasmic Foci | 0.0209 | 0.0029 | 0.0052 | 0.0315 | 0.0171 | 0.0069 | 0.0208 | 0.0012 | 0.008 | 0.0176 | 0.0041 | 0.0059 | 0.0188 | 0.0017 | 0.0064 | 0.0085 | 0.0051 | 0.0162 |
Eisosomes | 0.0004 | 0.0001 | 0.0004 | 0 | 0.0001 | 0.0001 | 0.0001 | 0 | 0.0001 | 0.0001 | 0 | 0 | 0.0001 | 0 | 0.0001 | 0.0002 | 0.0002 | 0 |
Endoplasmic Reticulum | 0.0066 | 0.0041 | 0.0052 | 0.0016 | 0.0106 | 0.0021 | 0.0077 | 0.0048 | 0.0051 | 0.0057 | 0.0027 | 0.0074 | 0.0065 | 0.0046 | 0.0039 | 0.0031 | 0.0045 | 0.0023 |
Endosome | 0.0211 | 0.0028 | 0.0078 | 0.0419 | 0.04 | 0.0037 | 0.0203 | 0.0018 | 0.0214 | 0.0146 | 0.0089 | 0.013 | 0.0068 | 0.004 | 0.0104 | 0.0056 | 0.0143 | 0.003 |
Golgi | 0.0063 | 0.0001 | 0.0028 | 0.0054 | 0.006 | 0.0013 | 0.0077 | 0 | 0.0134 | 0.0039 | 0.0001 | 0.0012 | 0.0003 | 0.0001 | 0.0042 | 0.0034 | 0.0112 | 0.0009 |
Lipid Particles | 0.0071 | 0.0003 | 0.0031 | 0.0152 | 0.0137 | 0.005 | 0.0283 | 0.0002 | 0.003 | 0.0096 | 0.0001 | 0.0033 | 0.0007 | 0.0003 | 0.0047 | 0.0032 | 0.0054 | 0.0005 |
Mitochondria | 0.0038 | 0.0005 | 0.0058 | 0.0014 | 0.0038 | 0.0021 | 0.0125 | 0.0004 | 0.0373 | 0.0056 | 0.0002 | 0.0012 | 0.0005 | 0.0004 | 0.0056 | 0.0007 | 0.0123 | 0.0004 |
None | 0.1843 | 0.2182 | 0.1749 | 0.1571 | 0.0835 | 0.2047 | 0.1523 | 0.1401 | 0.1244 | 0.1149 | 0.18 | 0.0666 | 0.1053 | 0.0718 | 0.1386 | 0.1074 | 0.126 | 0.0209 |
Nuclear Periphery | 0.0436 | 0.0135 | 0.0243 | 0.007 | 0.0671 | 0.0118 | 0.04 | 0.0272 | 0.0314 | 0.0375 | 0.0279 | 0.0382 | 0.0296 | 0.0318 | 0.0201 | 0.0219 | 0.026 | 0.0154 |
Nucleolus | 0.0041 | 0.0041 | 0.0075 | 0.0147 | 0.0078 | 0.0063 | 0.0045 | 0.0015 | 0.0029 | 0.0017 | 0.0009 | 0.0016 | 0.0018 | 0.0022 | 0.0023 | 0.0014 | 0.0041 | 0.0027 |
Nucleus | 0.468 | 0.5507 | 0.5869 | 0.4559 | 0.5688 | 0.5838 | 0.4846 | 0.6012 | 0.5563 | 0.6051 | 0.518 | 0.4511 | 0.6517 | 0.7184 | 0.6237 | 0.6661 | 0.6335 | 0.8036 |
Peroxisomes | 0.0068 | 0.0001 | 0.0016 | 0.0043 | 0.0059 | 0.0045 | 0.0081 | 0 | 0.0068 | 0.0329 | 0 | 0.0007 | 0.0002 | 0.0001 | 0.0027 | 0.0039 | 0.0014 | 0.0019 |
Punctate Nuclear | 0.0516 | 0.0228 | 0.0224 | 0.0758 | 0.0506 | 0.0508 | 0.021 | 0.0052 | 0.0122 | 0.0088 | 0.0026 | 0.0249 | 0.0146 | 0.0117 | 0.014 | 0.0196 | 0.0153 | 0.0335 |
Vacuole | 0.0026 | 0.0023 | 0.0025 | 0.0047 | 0.0066 | 0.001 | 0.0073 | 0.0021 | 0.0071 | 0.0041 | 0.0056 | 0.0059 | 0.0029 | 0.0033 | 0.0028 | 0.0012 | 0.0029 | 0.0012 |
Vacuole Periphery | 0.0014 | 0.0003 | 0.0009 | 0.0006 | 0.0027 | 0.0003 | 0.0046 | 0.0005 | 0.0018 | 0.0027 | 0.0008 | 0.0017 | 0.0006 | 0.0005 | 0.0011 | 0.0004 | 0.0014 | 0.0002 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 6.4266 | 8.8385 | 9.182 | 6.7065 | 7.6915 | 1.9763 | 8.4898 | 8.7458 | 9.1305 | 7.5168 |
Translational Efficiency | – | – | – | – | – | – | – | – | – | – |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✔ |
Translational Efficiency | – |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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