Standard name
Human Ortholog
Description Beta subunit of heterooctameric phosphofructokinase; involved in glycolysis; indispensable for anaerobic growth; activated by fructose-2,6-bisphosphate and AMP; mutation inhibits glucose induction of cell cycle-related genes
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0.05 0.06 0.05 0.07 0.06 0.11 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 1.0 1.0 1.0 0.99 0.99 0.95 0.92 0.92 0.94 0.91 0.87 0.87 0.99 1.0 0.98 1.0 0.98 0.99 0.98 0.95 0.95 0.91 0.94 0.93
Endoplasmic Reticulum 0 0 0 0 0 0 0.06 0.06 0 0.07 0.08 0.08 0 0 0 0 0.09 0.08 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 1 3 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 1 0 0 0 2 1 4 0 1 1 0 0 0 0 0 0 0 0 0 0 0 1
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 1 1 2 2 5 14 23 30 16 27 21 39 0 0 1 0 2 2 0 0 0 0 0 0
Cytoplasm 166 152 259 272 301 386 413 464 331 366 299 301 138 253 247 173 167 170 162 148 263 104 248 174
Endoplasmic Reticulum 0 0 2 1 1 4 27 28 11 28 28 27 1 0 4 2 15 14 0 0 3 1 4 4
Endosome 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 3 0 1 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 1 0 0 0 1 1 0 0 0 0 0 0 0 0 1 0 1 1 1
Nucleus 0 0 0 0 0 1 0 0 1 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 1 0 0 0 1 0 0 2 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 1 0 5 0 0 0 0 0 0 1 0 1 0 0 0 0 1 2 1 1 0
Unique Cell Count 166 152 260 274 303 405 447 504 353 404 342 347 140 253 253 173 170 171 166 156 276 115 265 187
Labelled Cell Count 167 153 264 277 308 414 465 525 363 422 353 371 140 253 254 175 185 186 166 156 276 115 265 187


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 262.7 207.1 158.6 143.0 152.2 138.8 132.4 120.2 114.7 108.8 98.9 100.0 223.0 210.7 177.1 231.3 256.1 246.7 221.3 220.6 247.9
Std Deviation (1e-4) 50.7 48.3 34.9 39.0 37.3 34.6 34.1 31.4 30.8 30.3 29.9 27.8 51.6 52.2 49.5 58.2 67.3 76.2 68.0 75.0 72.7
Intensity Change (Log2) – – – -0.15 -0.06 -0.19 -0.26 -0.4 -0.47 -0.54 -0.68 -0.67 0.49 0.41 0.16 0.54 0.69 0.64 0.48 0.48 0.64


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 2.2 3.0 3.4 2.7 3.6 3.4 5.1 0 0 0 0 0 0
Cytoplasm -0.5 -0.4 -3.2 -4.3 -4.4 -3.8 -4.8 -5.7 -5.9 -1.2 1.0 -1.9 0.8 -1.5 -0.3
Endoplasmic Reticulum 0 0 0 3.4 3.2 2.0 3.7 4.1 4.0 0 0 0 0 4.2 4.0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 188.1146 277.7297 231.2347 233.1001 219.4531 254.8668 190.6088 270.1877 246.4531 192.9314 184.3623 216.6846 197.0115 241.9961 225.4994 221.2935 187.8876 204.5577
Actin 0.0023 0.0003 0.0015 0.0005 0.0005 0.0003 0.0053 0.0002 0.0007 0.0086 0.0084 0.0027 0.0049 0.0003 0.0004 0.0022 0.0314 0.0073
Bud 0.0022 0.0007 0.0014 0.002 0.0018 0.0013 0.0022 0.0003 0.0012 0.0052 0.0036 0.001 0.0009 0.0004 0.0015 0.0031 0.0024 0.0015
Bud Neck 0.0009 0.0013 0.0023 0.0014 0.0048 0.0039 0.0006 0.0003 0.0011 0.0006 0.001 0.0019 0.0007 0.0004 0.0006 0.0011 0.0007 0.0007
Bud Periphery 0.0024 0.0005 0.0013 0.0031 0.0006 0.0016 0.003 0.0001 0.0008 0.0027 0.0035 0.0004 0.001 0.0002 0.0007 0.0014 0.0009 0.001
Bud Site 0.0011 0.0009 0.0009 0.0008 0.0003 0.0003 0.0014 0.0002 0.0005 0.0008 0.0007 0.0001 0.0005 0.0003 0.0003 0.0007 0.0005 0.0001
Cell Periphery 0.0745 0.0467 0.0536 0.0151 0.0085 0.036 0.0248 0.0075 0.0155 0.0302 0.0076 0.0018 0.0162 0.0042 0.0095 0.0123 0.0027 0.0038
Cytoplasm 0.499 0.8131 0.6185 0.5524 0.6469 0.6656 0.7458 0.9651 0.8809 0.6519 0.7207 0.9183 0.8112 0.9767 0.8967 0.8352 0.8119 0.8825
Cytoplasmic Foci 0.0184 0.0003 0.0068 0.0004 0.0587 0.0008 0.0027 0.0003 0.0025 0.0053 0.0044 0.0026 0.0014 0.0006 0.0042 0.0016 0.0111 0.0088
Eisosomes 0.0009 0.0001 0.0005 0 0.0003 0 0.0012 0 0.0002 0.0012 0.0026 0 0.0006 0 0 0.0001 0.0004 0
Endoplasmic Reticulum 0.0224 0.0073 0.0134 0.006 0.0031 0.0239 0.0402 0.001 0.0083 0.0027 0.0117 0.0143 0.0303 0.0031 0.0061 0.006 0.0011 0.0095
Endosome 0.0088 0.0005 0.0022 0.0058 0.0033 0.0031 0.0077 0.0003 0.0014 0.0155 0.0276 0.004 0.0029 0.0005 0.0027 0.0043 0.004 0.0151
Golgi 0.0015 0.0001 0.0004 0.0008 0.0003 0.0005 0.0013 0 0.0002 0.0035 0.0033 0.0007 0.0013 0.0002 0.0003 0.0006 0.0047 0.0041
Lipid Particles 0.0095 0.0009 0.0037 0.0004 0.0063 0.0027 0.0029 0.0002 0.0012 0.0087 0.0063 0.0007 0.0013 0.0005 0.0008 0.002 0.0093 0.0002
Mitochondria 0.028 0.0005 0.013 0.0081 0.012 0.001 0.0193 0.0001 0.0007 0.0252 0.0297 0.0031 0.0047 0.0001 0.0018 0.0029 0.0136 0.0055
None 0.0117 0.0002 0.0038 0.0002 0.0135 0.0006 0.0071 0.0002 0.0018 0.0067 0.0058 0.0016 0.0025 0.0002 0.0018 0.0006 0.0053 0.0009
Nuclear Periphery 0.0549 0.0159 0.0265 0.0179 0.0076 0.0206 0.0438 0.0016 0.0092 0.0136 0.0488 0.0116 0.0223 0.002 0.0048 0.0068 0.0039 0.0091
Nucleolus 0.032 0.0005 0.0185 0.0009 0.0115 0.0011 0.0063 0.0001 0.0021 0.0073 0.0063 0.0003 0.0024 0.0001 0.0036 0.0006 0.0086 0.0001
Nucleus 0.0241 0.0063 0.0147 0.0135 0.0095 0.0086 0.0165 0.0019 0.0037 0.0122 0.0123 0.0122 0.0078 0.0014 0.0051 0.005 0.006 0.0024
Peroxisomes 0.003 0 0.0005 0.0001 0.0015 0.0001 0.0003 0 0.0001 0.0012 0.0006 0.0001 0.0002 0 0.0001 0.0002 0.0044 0.0002
Punctate Nuclear 0.0011 0 0.0003 0 0.0014 0.0001 0.0005 0 0.0003 0.0006 0.0007 0.0002 0.0001 0 0.0002 0.0001 0.0008 0
Vacuole 0.1609 0.0969 0.1804 0.3022 0.1734 0.1886 0.0526 0.0194 0.0611 0.1534 0.0563 0.0159 0.0671 0.0079 0.0497 0.0903 0.0549 0.0261
Vacuole Periphery 0.0405 0.0069 0.0359 0.0682 0.0341 0.0394 0.0145 0.001 0.0065 0.043 0.0383 0.0064 0.0197 0.0008 0.0091 0.023 0.0212 0.0212

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 519.9027 538.0545 627.9889 648.242 577.2122 510.8079 695.9624 728.2854 762.4531 673.5594
Translational Efficiency 1.1864 1.3232 1.275 1.2556 1.2273 1.3947 1.2795 1.1701 1.2013 1.2614

Hit Data

Dataset Hit
Protein Concentration ✔
Protein Localization ✘
Gene Expression ✔
Translational Efficiency ✘
Standard name
Human Ortholog
Description Beta subunit of heterooctameric phosphofructokinase; involved in glycolysis; indispensable for anaerobic growth; activated by fructose-2,6-bisphosphate and AMP; mutation inhibits glucose induction of cell cycle-related genes
Localization
Cell Percentages cytoplasm (100%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-1

Pfk2

Pfk2


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Pfk2-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available