Standard name
Human Ortholog
Description Putative 1,3-beta-glucanosyltransferase; has similarity go other GAS family members; low abundance, possibly inactive member of the GAS family of GPI-containing proteins; localizes to the cell wall; mRNA induced during sporulation
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0.08 0.09 0.05 0.08 0.1 0.05 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0.06 0 0.06 0 0.06 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0.05 0.09 0.07 0.05 0 0.08 0.06 0 0.05 0.07 0.08 0.11 0 0 0 0 0 0 0 0 0
Cytoplasm 0.63 0.58 0.66 0.55 0.45 0.37 0.38 0.43 0.47 0.53 0.69 0.68 0.39 0.58 0.64 0.36 0.44 0.35 0 0.08 0.08 0.07 0.11 0.05
Endoplasmic Reticulum 0.32 0.39 0.28 0.44 0.43 0.48 0.47 0.34 0.36 0.24 0.14 0.13 0.57 0.4 0.31 0 0 0 0.64 0.63 0.66 0.81 0.74 0.59
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.05 0 0 0 0 0 0.1 0.08 0.09 0.11 0.1 0.1 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.23 0.18 0.22 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.05 0.05 0 0 0.08 0.06 0.09 0.1 0.05 0.1 0.08 0.13 0 0 0 0.62 0.6 0.55 0.3 0.21 0.17 0.06 0.1 0.26
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 1 3 8 24 28 19 32 35 21 12 10 7 9 6 2 0 0 0 3 4 3 2 9
Bud 0 1 7 2 5 20 10 26 13 24 11 10 3 4 3 1 0 0 0 3 3 2 1 1
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 1 0 1 3
Bud Site 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 4 6 10 26 22 20 15 29 24 9 14 13 31 41 0 1 0 0 0 0 0 2 1
Cytoplasm 12 88 127 110 133 121 161 179 173 222 167 192 71 222 244 89 53 34 0 14 17 9 34 19
Endoplasmic Reticulum 6 60 53 88 129 156 198 142 132 100 33 36 104 155 120 4 4 2 39 110 151 118 236 210
Endosome 0 1 1 0 9 6 5 12 0 7 2 1 2 7 4 6 2 0 0 0 2 0 2 5
Golgi 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 5 5 1 0 3 0 0 2 3
Mitochondria 1 3 3 2 2 14 44 34 32 47 25 27 0 0 1 0 1 0 0 1 3 0 1 2
Nucleus 0 0 0 0 0 0 0 0 0 6 0 4 1 0 1 56 22 21 0 0 0 0 0 0
Nuclear Periphery 0 1 0 1 0 2 5 4 4 3 3 3 0 0 1 1 0 0 0 0 1 0 0 0
Nucleolus 0 0 0 0 0 0 1 1 0 0 0 0 1 0 1 1 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 1 0 0 0 1
SpindlePole 0 0 0 0 0 0 0 1 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 1 7 8 2 25 21 39 41 19 41 19 36 2 2 9 152 73 53 18 36 39 8 31 90
Unique Cell Count 19 152 192 200 298 328 420 421 368 419 243 282 184 383 384 244 121 97 62 177 229 146 321 353
Labelled Cell Count 20 166 208 223 353 391 502 487 437 498 281 333 204 430 431 317 161 111 62 177 229 146 321 353


Cytoplasm, Endoplasmic Reticulum

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 7.2 8.7 7.2 7.7 9.4 8.5 7.7 8.0 7.9 7.3 6.9 6.9 9.5 8.8 8.0 12.7 14.2 16.2 8.5 8.2 8.3
Std Deviation (1e-4) 1.3 1.9 1.6 1.5 1.8 1.6 1.8 1.6 1.6 1.6 1.5 1.4 2.2 1.9 1.8 2.8 3.2 3.3 1.9 1.4 1.5
Intensity Change (Log2) – – – 0.09 0.39 0.24 0.09 0.15 0.13 0.01 -0.07 -0.07 0.41 0.29 0.15 0.82 0.98 1.17 0.23 0.18 0.2


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 1.5 3.1 3.2 1.8 3.0 3.5 2.0 1.9 1.3 0 0 0 0 0 0
Bud 0 0 1.2 -0.9 1.3 -0.1 1.1 0.5 -0.1 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0.9 2.4 1.7 0.9 0.3 2.2 1.4 0.3 1.0 1.7 2.3 3.1 0 0 0
Cytoplasm -2.3 -4.7 -6.4 -6.4 -5.4 -4.3 -3.1 0.6 0.4 -5.4 -1.9 -0.6 -6.2 -3.9 -5.0
Endoplasmic Reticulum 3.4 3.5 4.5 4.6 1.5 2.0 -1.0 -3.6 -4.1 5.7 3.0 0.9 -8.0 -5.4 -5.2
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 1.7 3.8 3.1 3.3 4.0 3.7 3.5 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 7.1 6.1 6.7
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 1.8 1.1 2.2 2.4 0.5 2.4 1.6 3.2 0 0 -1.2 12.5 11.0 9.9

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 4.5147 6.1808 8.1301 7.4049 9.5768 7.8992 5.7912 6.1999 7.6828 7.9264 7.7363 7.5844 5.4098 7.8926 9.3677 11.4769 9.4117 8.7159
Actin 0.0389 0.0042 0.0066 0.0019 0.0002 0.0002 0.0582 0.0188 0.0087 0.052 0.0362 0.0057 0.0514 0.0136 0.0218 0.0087 0.0002 0.0018
Bud 0.0003 0.0002 0.0002 0.0001 0 0 0.0005 0.0006 0.0005 0.0003 0.0009 0 0.0003 0.0004 0.0001 0.0002 0.0038 0.0002
Bud Neck 0.0009 0.0006 0.0016 0.0013 0.0005 0.0008 0.0021 0.0006 0.0002 0.0003 0.0007 0.0028 0.0002 0.0003 0.0003 0.0164 0.0095 0.0022
Bud Periphery 0.0005 0.0003 0.001 0.0003 0.0001 0 0.0006 0.0008 0.0007 0.0004 0.0007 0.0001 0.0005 0.0006 0.0002 0.0008 0.0052 0.0003
Bud Site 0.0021 0.0032 0.0013 0.0002 0 0 0.0043 0.0245 0.0018 0.0005 0.0035 0.0002 0.0004 0.0024 0.0004 0.0004 0.0207 0.0002
Cell Periphery 0.003 0.0032 0.0088 0.0011 0.0014 0.0006 0.0007 0.008 0.0023 0.0004 0.0014 0.0014 0.0018 0.0026 0.002 0.008 0.0009 0.0014
Cytoplasm 0.0459 0.243 0.1034 0.1119 0.0049 0.0365 0.0473 0.1822 0.0534 0.0552 0.0414 0.1714 0.0976 0.2578 0.1684 0.1624 0.0555 0.094
Cytoplasmic Foci 0.0152 0.0122 0.0045 0.0014 0.0001 0.0003 0.034 0.0284 0.0118 0.0076 0.0105 0.0035 0.0078 0.0149 0.0013 0.0039 0.0119 0.0071
Eisosomes 0.0005 0.0002 0.0001 0 0 0 0.0002 0.0003 0.0001 0.0002 0 0 0.0007 0.0003 0.0001 0.0001 0 0
Endoplasmic Reticulum 0.686 0.5518 0.8082 0.8507 0.9768 0.9395 0.6964 0.6001 0.8568 0.7539 0.8396 0.7865 0.6902 0.5748 0.7771 0.7722 0.8223 0.7931
Endosome 0.0223 0.0393 0.01 0.0051 0.0013 0.0031 0.0308 0.0166 0.0128 0.0548 0.0206 0.0041 0.0262 0.035 0.0037 0.0044 0.025 0.0178
Golgi 0.012 0.0032 0.007 0.0007 0.0003 0.0002 0.0165 0.0101 0.0077 0.013 0.0132 0.001 0.0181 0.0024 0.0011 0.002 0.0032 0.0075
Lipid Particles 0.0527 0.0121 0.0131 0.002 0.0006 0.0014 0.0193 0.0234 0.008 0.0101 0.0128 0.0043 0.0074 0.0159 0.0039 0.0083 0.0017 0.0046
Mitochondria 0.0322 0.0011 0.0018 0.0002 0.0001 0.0001 0.0046 0.0044 0.0008 0.0036 0.0015 0.0001 0.0049 0.0154 0.0002 0.0001 0.0008 0.0041
None 0.0049 0.0155 0.0022 0.0003 0 0.0002 0.0086 0.0547 0.0032 0.0114 0.0002 0.0041 0.053 0.006 0.0007 0.0002 0.0004 0.0003
Nuclear Periphery 0.0276 0.0439 0.0121 0.0133 0.0068 0.01 0.0345 0.0051 0.005 0.0216 0.0063 0.0038 0.0208 0.011 0.0116 0.0068 0.0069 0.0119
Nucleolus 0.0006 0.0002 0.0004 0 0 0 0.0009 0.0002 0.0001 0.0004 0 0 0.0004 0.0003 0 0 0.001 0.0001
Nucleus 0.0024 0.011 0.002 0.0014 0.0004 0.0012 0.0089 0.0011 0.0007 0.0036 0.0005 0.0007 0.0032 0.0023 0.0008 0.0009 0.0031 0.0027
Peroxisomes 0.0272 0.0004 0.0015 0 0 0 0.0125 0.0059 0.015 0.0017 0.0031 0.0002 0.0004 0.0008 0 0.0001 0.0005 0.0027
Punctate Nuclear 0.0086 0.0012 0.0056 0.0002 0 0.0001 0.0075 0.0011 0.0008 0.002 0.0018 0.0002 0.0052 0.0128 0.0001 0.0004 0.0007 0.0002
Vacuole 0.014 0.05 0.0076 0.0071 0.0061 0.0053 0.0094 0.0127 0.0092 0.0055 0.0047 0.0095 0.0076 0.0297 0.0058 0.0035 0.0252 0.046
Vacuole Periphery 0.0021 0.003 0.0007 0.0007 0.0004 0.0004 0.0022 0.0004 0.0005 0.0018 0.0004 0.0004 0.0017 0.0009 0.0004 0.0002 0.0015 0.0018

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 62.3511 126.5067 52.5133 42.2015 69.941 47.6127 125.2196 100.6484 54.2108 65.5158
Translational Efficiency 0.9131 1.0484 1.1975 0.9718 0.9101 1.0587 1.0525 0.9223 1.0223 0.99

Hit Data

Dataset Hit
Protein Concentration ✔
Protein Localization ✔
Gene Expression ✔
Translational Efficiency ✘
Standard name
Human Ortholog
Description Putative 1,3-beta-glucanosyltransferase; has similarity go other GAS family members; low abundance, possibly inactive member of the GAS family of GPI-containing proteins; localizes to the cell wall; mRNA induced during sporulation
Localization
Cell Percentages ER (95%)
Cell Cycle Regulation No
Subcompartmental Group ER-3

Gas3

Gas3


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Gas3-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available