ORF | |
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Human Ortholog | |
Description | NADP(+)-dependent serine dehydrogenase and carbonyl reductase; acts on serine, L-allo-threonine, and other 3-hydroxy acids; green fluorescent protein fusion protein localizes to the cytoplasm and nucleus; may interact with ribosomes, based on co-purification experiments |
ensLOC | DeepLoc | |||||||||||||||||||||||
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Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 1 | 0 | 0 | 2 | 3 | 2 | 8 | 4 | 3 | 9 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 1 | 1 | 0 | 1 | 0 | 2 | 1 | 1 | 2 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 2 | 1 | 3 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 1 | 2 | 1 | 4 | 6 | 3 | 15 | 10 | 8 | 10 | 8 | 0 | 2 | 4 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 78 | 284 | 359 | 304 | 306 | 420 | 347 | 531 | 289 | 285 | 260 | 249 | 206 | 338 | 417 | 201 | 156 | 119 | 93 | 291 | 339 | 177 | 266 | 289 |
Endoplasmic Reticulum | 0 | 5 | 2 | 1 | 8 | 19 | 11 | 66 | 26 | 19 | 6 | 6 | 3 | 1 | 3 | 7 | 5 | 5 | 0 | 1 | 1 | 0 | 1 | 5 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 3 | 4 | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 2 | 0 | 0 | 1 | 1 | 1 | 6 | 0 | 14 | 11 | 5 | 17 | 0 | 0 | 0 | 2 | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 0 |
Nucleus | 0 | 0 | 2 | 0 | 2 | 3 | 13 | 12 | 2 | 1 | 6 | 5 | 2 | 2 | 3 | 0 | 1 | 0 | 1 | 4 | 1 | 4 | 7 | 4 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 2 |
Nucleolus | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vac/Vac Membrane | 0 | 3 | 2 | 11 | 19 | 9 | 16 | 43 | 11 | 34 | 18 | 39 | 0 | 3 | 4 | 4 | 0 | 3 | 1 | 1 | 1 | 0 | 2 | 4 |
Unique Cell Count | 80 | 291 | 363 | 313 | 335 | 453 | 386 | 650 | 350 | 357 | 303 | 319 | 211 | 342 | 422 | 203 | 156 | 122 | 102 | 303 | 354 | 190 | 288 | 318 |
Labelled Cell Count | 80 | 293 | 368 | 319 | 343 | 460 | 400 | 670 | 365 | 367 | 309 | 336 | 211 | 348 | 431 | 216 | 163 | 127 | 102 | 303 | 354 | 190 | 288 | 318 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 19.3 | 15.4 | 19.0 | 20.1 | 17.4 | 20.7 | 12.6 | 19.5 | 17.8 | 18.1 | 17.9 | 16.5 | 21.8 | 19.5 | 19.4 | 21.3 | 25.7 | 30.3 | 20.4 | 21.6 | 22.6 |
Std Deviation (1e-4) | 4.8 | 3.5 | 3.2 | 3.8 | 4.3 | 4.0 | 3.5 | 3.8 | 3.6 | 3.7 | 3.5 | 3.5 | 4.9 | 4.4 | 3.7 | 5.0 | 5.6 | 9.0 | 6.2 | 5.6 | 6.3 |
Intensity Change (Log2) | – | – | – | 0.08 | -0.13 | 0.13 | -0.59 | 0.04 | -0.09 | -0.07 | -0.08 | -0.21 | 0.2 | 0.04 | 0.03 | 0.17 | 0.44 | 0.67 | 0.1 | 0.19 | 0.25 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 2.1 | 2.4 | 0 | 2.7 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | -1.7 | -4.7 | -4.2 | -5.3 | -8.0 | -7.6 | -8.3 | -6.6 | -8.7 | -1.2 | -0.1 | -0.1 | 0.1 | 1.3 | -1.1 |
Endoplasmic Reticulum | 0 | 0 | 3.3 | 2.4 | 5.9 | 4.7 | 3.8 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 0 | 0 | 0 | 3.8 | 3.4 | 0 | 4.5 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 0 | 0 | 0 | 2.8 | 1.7 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 2.8 | 4.0 | 0 | 3.2 | 4.5 | 2.6 | 5.5 | 4.1 | 6.4 | 0 | 0 | 0 | 0 | 0 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 14.7122 | 21.1429 | 17.3765 | 17.231 | 14.2989 | 17.7609 | 17.9598 | 23.3656 | 21.346 | 20.5422 | 18.8453 | 21.1469 | 16.2655 | 20.7426 | 18.5368 | 17.0193 | 17.9137 | 19.0673 |
Actin | 0.0264 | 0.0001 | 0.0052 | 0.0002 | 0.0524 | 0.0074 | 0.0271 | 0.0001 | 0.0014 | 0.0001 | 0.0026 | 0.0024 | 0.029 | 0.0002 | 0.0084 | 0.0043 | 0.0035 | 0.0011 |
Bud | 0.0005 | 0 | 0.0004 | 0.0001 | 0.0004 | 0.0001 | 0.0003 | 0.0001 | 0.0001 | 0 | 0.0001 | 0.0001 | 0.0005 | 0 | 0.0002 | 0.0013 | 0.0004 | 0.0002 |
Bud Neck | 0.001 | 0.0001 | 0.0003 | 0.0002 | 0.0008 | 0.0008 | 0.0011 | 0.0001 | 0.0003 | 0.0004 | 0.0005 | 0.0008 | 0.0013 | 0.0001 | 0.0004 | 0.0013 | 0.001 | 0.0014 |
Bud Periphery | 0.0004 | 0 | 0.0003 | 0 | 0.0006 | 0.0003 | 0.0003 | 0 | 0 | 0 | 0 | 0.0001 | 0.0005 | 0 | 0.0003 | 0.0019 | 0.0005 | 0.0001 |
Bud Site | 0.0015 | 0 | 0.0003 | 0 | 0.0011 | 0.0001 | 0.0016 | 0.0001 | 0.0001 | 0 | 0 | 0.0001 | 0.0022 | 0.0002 | 0.0011 | 0.0024 | 0.0007 | 0.0001 |
Cell Periphery | 0.0001 | 0 | 0 | 0 | 0.0001 | 0 | 0.0003 | 0 | 0 | 0 | 0 | 0 | 0.0002 | 0 | 0.0001 | 0.0004 | 0.0003 | 0.0001 |
Cytoplasm | 0.8344 | 0.9843 | 0.8856 | 0.9271 | 0.8469 | 0.8785 | 0.8445 | 0.987 | 0.9577 | 0.9668 | 0.9374 | 0.9484 | 0.8278 | 0.9873 | 0.9076 | 0.8551 | 0.8714 | 0.9148 |
Cytoplasmic Foci | 0.0106 | 0.0002 | 0.014 | 0.0004 | 0.0178 | 0.0011 | 0.0126 | 0.0003 | 0.0012 | 0.0004 | 0.0075 | 0.0023 | 0.0153 | 0.0006 | 0.0089 | 0.0296 | 0.0158 | 0.0017 |
Eisosomes | 0.0002 | 0 | 0.0001 | 0 | 0.0002 | 0.0001 | 0.0007 | 0 | 0 | 0 | 0 | 0 | 0.0002 | 0 | 0.0006 | 0 | 0.0001 | 0 |
Endoplasmic Reticulum | 0.0044 | 0.0009 | 0.0028 | 0.0004 | 0.001 | 0.0046 | 0.007 | 0.0007 | 0.0017 | 0.0007 | 0.0016 | 0.0022 | 0.0077 | 0.0006 | 0.0054 | 0.0081 | 0.0243 | 0.0067 |
Endosome | 0.0115 | 0.0001 | 0.0087 | 0.0001 | 0.0102 | 0.008 | 0.0158 | 0.0003 | 0.0012 | 0.0002 | 0.0099 | 0.0078 | 0.0212 | 0.0003 | 0.0108 | 0.0451 | 0.0311 | 0.002 |
Golgi | 0.0033 | 0 | 0.0018 | 0 | 0.0089 | 0.0037 | 0.0047 | 0 | 0.0001 | 0 | 0.0024 | 0.0011 | 0.0073 | 0 | 0.0021 | 0.0086 | 0.0037 | 0.0001 |
Lipid Particles | 0.0068 | 0 | 0.0027 | 0 | 0.0149 | 0.0035 | 0.0055 | 0 | 0 | 0 | 0.0006 | 0.0007 | 0.0042 | 0 | 0.0031 | 0.0026 | 0.0019 | 0.0002 |
Mitochondria | 0.004 | 0 | 0.0043 | 0.0001 | 0.0038 | 0.0092 | 0.0057 | 0 | 0.0001 | 0 | 0.0009 | 0.0006 | 0.002 | 0 | 0.0089 | 0.0071 | 0.0034 | 0.0005 |
None | 0.002 | 0.0002 | 0.0015 | 0.0005 | 0.0011 | 0.0006 | 0.005 | 0.0004 | 0.0008 | 0.0004 | 0.0007 | 0.0006 | 0.0165 | 0.0004 | 0.0077 | 0.0008 | 0.013 | 0.0009 |
Nuclear Periphery | 0.0128 | 0.0014 | 0.0118 | 0.0045 | 0.0039 | 0.018 | 0.0125 | 0.0012 | 0.0024 | 0.0016 | 0.0038 | 0.0027 | 0.0151 | 0.0009 | 0.0043 | 0.004 | 0.0098 | 0.0107 |
Nucleolus | 0.0006 | 0 | 0.0002 | 0 | 0.0001 | 0.0002 | 0.0004 | 0 | 0 | 0 | 0 | 0 | 0.0004 | 0 | 0.0002 | 0.0001 | 0.0005 | 0.0001 |
Nucleus | 0.0645 | 0.0122 | 0.0536 | 0.0654 | 0.0175 | 0.0605 | 0.0357 | 0.0094 | 0.0318 | 0.0288 | 0.0295 | 0.0283 | 0.0372 | 0.0087 | 0.0236 | 0.0154 | 0.0117 | 0.0568 |
Peroxisomes | 0.007 | 0 | 0.002 | 0 | 0.0129 | 0.0004 | 0.0054 | 0 | 0 | 0 | 0.0012 | 0.0001 | 0.0019 | 0 | 0.0025 | 0.0021 | 0.0008 | 0 |
Punctate Nuclear | 0.0031 | 0.0001 | 0.003 | 0.0003 | 0.004 | 0.0012 | 0.0104 | 0.0001 | 0.0006 | 0.0003 | 0.0008 | 0.0008 | 0.0072 | 0.0001 | 0.0012 | 0.0023 | 0.001 | 0.0014 |
Vacuole | 0.0038 | 0.0002 | 0.0009 | 0.0003 | 0.0009 | 0.0008 | 0.0019 | 0.0002 | 0.0004 | 0.0001 | 0.0004 | 0.0006 | 0.0015 | 0.0004 | 0.002 | 0.0058 | 0.0039 | 0.0008 |
Vacuole Periphery | 0.001 | 0 | 0.0006 | 0.0001 | 0.0005 | 0.0009 | 0.0015 | 0 | 0.0001 | 0 | 0.0002 | 0.0003 | 0.0007 | 0 | 0.0007 | 0.0016 | 0.0013 | 0.0004 |
R1 | R2 | |||||||||
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G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 192.1351 | 144.7667 | 143.8377 | 160.9032 | 150.3566 | 197.5153 | 178.005 | 192.9797 | 203.2157 | 191.1638 |
Translational Efficiency | 2.1771 | 2.3024 | 1.829 | 1.5632 | 2.0483 | 2.4252 | 1.8401 | 1.4398 | 1.6693 | 1.7839 |
Dataset | Hit |
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Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✔ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
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37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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