Standard name | |
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Human Ortholog | |
Description | Glutamate decarboxylase; converts glutamate into gamma-aminobutyric acid (GABA) during glutamate catabolism; involved in response to oxidative stress |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | – | 1 | 0 | 0 | 0 | – | 2 | 2 | 1 | 0 | – | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 1 | 2 | – | 1 | 0 | 0 | 0 | – | 3 | 6 | 2 | 3 | – | – | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 1 |
Bud Neck | 0 | 1 | – | 1 | 2 | 1 | 3 | – | 3 | 2 | 0 | 2 | – | – | 0 | 0 | 1 | 1 | 0 | 1 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | – | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 1 | – | – | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 1 | 0 | – | 1 | 2 | 1 | 1 | – | 0 | 1 | 0 | 2 | – | – | 0 | 19 | 11 | 28 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 92 | 155 | – | 65 | 173 | 110 | 178 | – | 60 | 141 | 73 | 125 | – | – | 18 | 328 | 271 | 335 | 78 | 138 | 14 | 7 | 25 | 40 |
Endoplasmic Reticulum | 2 | 1 | – | 0 | 1 | 0 | 4 | – | 2 | 1 | 3 | 3 | – | – | 1 | 29 | 12 | 29 | 0 | 1 | 0 | 0 | 0 | 0 |
Endosome | 0 | 0 | – | 0 | 1 | 0 | 2 | – | 1 | 1 | 0 | 1 | – | – | 0 | 8 | 2 | 5 | 2 | 2 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | – | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | – | – | 1 | 3 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 27 | 0 | – | 5 | 0 | 5 | 7 | – | 32 | 40 | 51 | 68 | – | – | 0 | 5 | 2 | 4 | 18 | 9 | 1 | 0 | 0 | 1 |
Nucleus | 1 | 1 | – | 2 | 0 | 0 | 2 | – | 3 | 3 | 1 | 3 | – | – | 0 | 0 | 3 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | – | 0 | 0 | 0 | 0 | – | 1 | 3 | 2 | 0 | – | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | – | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | – | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | – | 0 | 0 | 1 | 0 | – | 0 | 0 | 0 | 0 | – | – | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 1 | – | 0 | 1 | 0 | 5 | – | 0 | 2 | 2 | 3 | – | – | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
Vac/Vac Membrane | 0 | 0 | – | 0 | 0 | 1 | 2 | – | 0 | 6 | 2 | 9 | – | – | 0 | 12 | 3 | 4 | 1 | 7 | 1 | 0 | 0 | 1 |
Unique Cell Count | 105 | 158 | 67 | 174 | 116 | 194 | 85 | 182 | 112 | 187 | 20 | 351 | 280 | 348 | 108 | 163 | 17 | 8 | 26 | 49 | ||||
Labelled Cell Count | 124 | 161 | 76 | 180 | 119 | 204 | 107 | 208 | 137 | 220 | 20 | 406 | 308 | 409 | 108 | 163 | 17 | 8 | 26 | 49 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 3.5 | 4.9 | – | 4.7 | 5.6 | 4.7 | 4.4 | – | 4.0 | 4.5 | 4.1 | 4.6 | – | – | 7.4 | 6.5 | 6.4 | 6.0 | – | – | 6.4 |
Std Deviation (1e-4) | 0.5 | 4.4 | – | 1.4 | 2.0 | 2.3 | 1.1 | – | 2.0 | 1.6 | 1.9 | 2.1 | – | – | 2.9 | 1.5 | 1.6 | 1.4 | – | – | 2.7 |
Intensity Change (Log2) | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Actin | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud Neck | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud Site | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cell Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cyto | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endoplasmic Reticulum | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endosome | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Golgi | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Mitochondria | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nuclear Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nuc | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleolus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Peroxisomes | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
SpindlePole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Vac | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cortical Patches | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cytoplasm | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Vacuole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 0.0676 | -0.423 | -0.3499 | -0.5205 | 0.1674 | -0.3096 | 0.1353 | 0.7408 | 0.0828 | -0.1123 | -0.2371 | -0.7514 | -2.0257 | -1.6546 | -1.6728 | -2.0254 | -1.907 | -0.9871 |
Actin | 0.0379 | 0.0006 | 0.0063 | 0.0101 | 0.01 | 0.0039 | 0.0499 | 0.0004 | 0.0168 | 0.0339 | 0 | 0.0003 | 0.0391 | 0.0001 | 0.0188 | 0.0003 | 0.0051 | 0.001 |
Bud | 0.002 | 0.0076 | 0.0044 | 0.0007 | 0.0018 | 0.0058 | 0.0008 | 0.0003 | 0.0016 | 0.0003 | 0 | 0 | 0.0031 | 0.0001 | 0.0021 | 0 | 0.0002 | 0.0026 |
Bud Neck | 0.002 | 0.0004 | 0.0006 | 0.0002 | 0.0012 | 0.0013 | 0.0064 | 0.0001 | 0.0005 | 0.0008 | 0 | 0.0001 | 0.0012 | 0 | 0.0004 | 0 | 0.0004 | 0.0003 |
Bud Periphery | 0.0021 | 0.0099 | 0.0022 | 0.0014 | 0.0018 | 0.0056 | 0.0009 | 0.0002 | 0.0015 | 0.0005 | 0 | 0.0001 | 0.0036 | 0.0001 | 0.0026 | 0 | 0.0004 | 0.0078 |
Bud Site | 0.0102 | 0.0295 | 0.0072 | 0.0006 | 0.0081 | 0.0048 | 0.0086 | 0.0033 | 0.01 | 0.0012 | 0 | 0 | 0.0046 | 0.0004 | 0.0018 | 0.0001 | 0.0007 | 0.0002 |
Cell Periphery | 0.0004 | 0.0005 | 0.0005 | 0.0001 | 0.0002 | 0.0002 | 0.0005 | 0.0002 | 0.0002 | 0.0002 | 0 | 0.0003 | 0.0003 | 0.0001 | 0.0002 | 0 | 0.0001 | 0.0002 |
Cytoplasm | 0.0479 | 0.1134 | 0.0681 | 0.074 | 0.0602 | 0.1119 | 0.1088 | 0.2931 | 0.1708 | 0.2107 | 0.1361 | 0.2702 | 0.0722 | 0.1852 | 0.1059 | 0.0958 | 0.1375 | 0.1511 |
Cytoplasmic Foci | 0.025 | 0.0136 | 0.029 | 0.0121 | 0.0456 | 0.0159 | 0.0311 | 0.007 | 0.0176 | 0.0218 | 0.0012 | 0.0048 | 0.0276 | 0.0092 | 0.0087 | 0.0084 | 0.0195 | 0.0128 |
Eisosomes | 0.0007 | 0.0004 | 0.0005 | 0.0001 | 0.0002 | 0.0001 | 0.0008 | 0.0001 | 0.0002 | 0.0002 | 0.0001 | 0.0002 | 0.0003 | 0.0001 | 0.0002 | 0.0001 | 0.0001 | 0.0001 |
Endoplasmic Reticulum | 0.0027 | 0.001 | 0.0017 | 0.0007 | 0.0012 | 0.0012 | 0.0031 | 0.0017 | 0.002 | 0.0028 | 0.0002 | 0.0089 | 0.0018 | 0.0013 | 0.0012 | 0.001 | 0.0045 | 0.0007 |
Endosome | 0.0542 | 0.0016 | 0.0302 | 0.0154 | 0.0594 | 0.0107 | 0.0215 | 0.0052 | 0.0086 | 0.0704 | 0.0001 | 0.002 | 0.0092 | 0.0023 | 0.0034 | 0.0015 | 0.043 | 0.0063 |
Golgi | 0.0249 | 0.0002 | 0.0108 | 0.0146 | 0.0321 | 0.0056 | 0.0115 | 0.0008 | 0.0046 | 0.0147 | 0 | 0.0002 | 0.007 | 0.0001 | 0.0023 | 0.0001 | 0.0161 | 0.0018 |
Lipid Particles | 0.0221 | 0.0009 | 0.0088 | 0.0041 | 0.0254 | 0.0018 | 0.0183 | 0.0005 | 0.0032 | 0.011 | 0.0001 | 0.0003 | 0.0165 | 0.0002 | 0.0036 | 0.0004 | 0.0406 | 0.0107 |
Mitochondria | 0.0252 | 0.0011 | 0.0195 | 0.0671 | 0.0204 | 0.026 | 0.0284 | 0.0002 | 0.0027 | 0.0037 | 0 | 0.0005 | 0.0083 | 0.0002 | 0.0015 | 0.0001 | 0.009 | 0.0048 |
None | 0.6912 | 0.8034 | 0.781 | 0.7932 | 0.662 | 0.7899 | 0.6262 | 0.6828 | 0.7441 | 0.6086 | 0.8616 | 0.7098 | 0.7726 | 0.798 | 0.8266 | 0.8897 | 0.6971 | 0.789 |
Nuclear Periphery | 0.006 | 0.0008 | 0.001 | 0.0003 | 0.0029 | 0.0022 | 0.0059 | 0.0007 | 0.0006 | 0.0052 | 0.0001 | 0.0004 | 0.001 | 0.0004 | 0.0006 | 0.0003 | 0.0022 | 0.0007 |
Nucleolus | 0.0042 | 0.0013 | 0.0015 | 0.0001 | 0.004 | 0.0003 | 0.013 | 0.0002 | 0.0004 | 0.0003 | 0 | 0 | 0.0004 | 0.0001 | 0.0002 | 0.0001 | 0.0002 | 0.0012 |
Nucleus | 0.0049 | 0.0066 | 0.0031 | 0.0003 | 0.0046 | 0.0026 | 0.0077 | 0.0008 | 0.0008 | 0.0012 | 0.0001 | 0.0004 | 0.0018 | 0.0005 | 0.0005 | 0.0003 | 0.0005 | 0.0008 |
Peroxisomes | 0.0101 | 0.0008 | 0.0137 | 0.0026 | 0.0249 | 0.0047 | 0.0364 | 0.0001 | 0.0109 | 0.0032 | 0 | 0 | 0.0229 | 0.0001 | 0.0165 | 0.0002 | 0.0181 | 0.0032 |
Punctate Nuclear | 0.0154 | 0.0041 | 0.0049 | 0.0003 | 0.0164 | 0.0033 | 0.0165 | 0.001 | 0.001 | 0.0056 | 0.0001 | 0.0001 | 0.0046 | 0.0002 | 0.0022 | 0.0005 | 0.0016 | 0.0009 |
Vacuole | 0.0075 | 0.0017 | 0.0039 | 0.001 | 0.0104 | 0.0012 | 0.0028 | 0.0012 | 0.002 | 0.0031 | 0.0001 | 0.001 | 0.0016 | 0.001 | 0.0007 | 0.0009 | 0.0023 | 0.0024 |
Vacuole Periphery | 0.0033 | 0.0003 | 0.0012 | 0.0008 | 0.0072 | 0.0009 | 0.0011 | 0.0001 | 0.0003 | 0.0009 | 0 | 0.0002 | 0.0004 | 0.0001 | 0.0001 | 0.0001 | 0.0008 | 0.0013 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 13.4944 | 6.7329 | 7.9105 | 23.0629 | 7.7308 | 23.8374 | 12.3389 | 21.442 | 35.2766 | 18.6866 |
Translational Efficiency | 1.2671 | 0.9106 | 1.4321 | 1.1282 | 1.3705 | 1.08 | 0.9163 | 0.8753 | 0.9006 | 0.8659 |
Dataset | Hit |
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Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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