ORF
Human Ortholog
Description Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern; YMR253C is not an essential gene
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0.05 0 0 0 0 0 0 0 0 0 0.07 0 0 0.07 0 0.05 0 0 0 0 0 0
Bud 0 0 0 0 0 0.09 0.07 0.08 0.07 0.12 0.09 0.13 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0.05 0.08 0.07 0 0.07 0 0 0 0 0.06 0 0 0 0.05 0.05 0.06 0 0 0 0 0 0
Endoplasmic Reticulum 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0.06 0.06 0.15 0.22 0.41 0.1 0.06 0.22 0 0 0 0 0.13 0.12 0.15 0.33 0.3 0.28 0.24 0.22 0.18 0.3 0.28 0.18
Golgi 0.1 0.29 0.35 0.14 0.06 0 0 0 0 0 0 0 0.69 0.71 0.55 0.42 0.42 0.42 0.56 0.56 0.59 0.51 0.53 0.66
Mitochondria 0.9 0.87 0.66 0.72 0.52 0.8 0.78 0.68 0.88 0.88 0.9 0.87 0.41 0.24 0.36 0.07 0.08 0.08 0.05 0.12 0 0.07 0.08 0.07
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.09 0 0 0.05 0 0.05 0.05 0 0 0 0 0.07 0 0 0 0.06 0 0.05 0 0 0 0 0 0
Peroxisomes 0 0 0.05 0 0.12 0.05 0 0.09 0 0 0 0 0 0 0 0 0 0 0.1 0 0.1 0 0 0
SpindlePole 0 0 0 0 0.07 0 0.05 0.08 0 0 0 0 0 0 0 0.11 0.09 0.07 0 0 0 0 0 0
Vac/Vac Membrane 0.06 0.09 0 0.05 0.06 0.06 0.05 0 0.05 0 0 0 0.07 0.11 0.09 0.16 0.2 0.21 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 5 11 9 2 2 3 8 4 4 7 1 0 27 13 7 23 11 15 6 8 8 3 6 6
Bud 5 8 5 1 8 21 23 32 17 32 18 21 3 2 2 5 9 5 0 1 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 1 0 1 2 5 7 6 9 4 1 0 0 0 1 1 0 – – – – – –
Cell Periphery 1 1 1 0 0 1 6 2 0 3 3 1 1 4 2 2 5 2 0 0 0 0 0 0
Cytoplasm 3 6 9 8 12 7 23 12 6 11 7 10 16 17 18 17 14 20 0 0 1 3 2 2
Endoplasmic Reticulum 3 13 3 2 1 2 7 1 1 3 1 1 8 1 2 8 11 13 0 0 2 1 3 3
Endosome 16 14 29 23 74 25 19 82 7 7 5 1 50 55 68 108 86 90 63 59 37 93 129 79
Golgi 25 71 67 15 10 7 10 3 8 4 2 1 273 338 243 137 120 134 146 149 124 156 243 283
Mitochondria 225 212 126 76 93 191 250 257 204 232 183 137 164 113 156 23 24 26 12 31 6 21 35 28
Nucleus 0 0 1 0 2 0 0 0 0 3 0 2 0 3 2 2 2 0 0 0 0 1 0 0
Nuclear Periphery 2 1 0 0 0 0 2 1 1 1 2 1 3 1 1 1 4 2 0 0 0 0 1 0
Nucleolus 22 7 5 5 4 13 16 17 5 10 9 11 5 1 9 19 12 16 0 0 0 0 0 0
Peroxisomes 1 1 9 2 21 12 4 36 6 5 4 3 8 9 16 8 8 4 26 7 21 10 7 10
SpindlePole 9 0 4 0 12 8 16 29 5 11 8 4 7 9 15 37 26 23 2 4 2 1 4 2
Vac/Vac Membrane 14 23 3 5 11 15 17 12 12 8 8 1 26 54 41 53 56 68 1 5 2 11 15 9
Unique Cell Count 249 245 190 106 179 240 322 379 233 264 203 158 396 475 439 325 285 318 261 270 210 308 455 431
Labelled Cell Count 331 368 272 139 251 307 406 495 282 346 255 195 591 620 582 444 389 418 261 270 210 308 455 431


Early Golgi, Punctate Nuclear

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 7.5 8.0 8.7 7.4 7.4 6.8 6.9 6.3 6.1 6.1 5.7 6.0 10.8 11.0 10.4 10.3 9.9 9.8 9.2 9.5 10.0
Std Deviation (1e-4) 1.2 1.6 2.4 1.9 1.4 2.1 3.0 1.2 1.2 1.2 1.1 1.2 1.9 2.0 2.1 2.2 2.1 2.1 1.7 1.7 1.7
Intensity Change (Log2) – – – -0.24 -0.24 -0.36 -0.33 -0.48 -0.51 -0.53 -0.62 -0.54 0.3 0.34 0.24 0.24 0.18 0.17 0.08 0.12 0.19


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 -2.0 -2.2 -1.4 0 -1.8 -1.2 0 0 1.0 -1.3 0 1.1 -0.5 0
Bud 0 1.0 2.6 2.2 2.7 2.1 3.6 2.6 3.8 0 0 0 0 0.3 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 1.0 0.8 -1.0 1.1 -0.9 -1.2 -0.3 -0.6 0.7 -0.4 -0.7 -0.4 0.2 0.1 0.7
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 1.4 1.6
Endosome 1.4 5.6 -1.5 -3.5 1.8 -4.5 -4.9 -4.5 -4.8 -0.9 -1.3 0.1 4.5 3.7 3.4
Golgi -3.9 -7.0 -8.8 -9.8 -11.8 -8.5 -9.8 -8.9 -8.1 7.7 8.6 4.6 1.5 1.5 1.5
Mitochondria 1.0 -2.8 3.1 2.8 0.4 5.2 5.6 5.8 4.4 -5.6 -10.3 -7.1 -14.3 -13.3 -13.8
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 1.4 1.3 1.1 0 0.7 1.0 1.9 0 0 0 1.7 0.9 1.3
Peroxisomes 0 2.5 0.1 0 2.0 -1.2 -1.7 -1.5 -1.4 -1.8 -2.0 -0.6 -1.4 -1.1 0
SpindlePole 0 2.2 0.8 1.6 2.7 0 1.2 1.1 0 0 0 0.9 3.8 3.1 2.5
Vacuole 0 2.3 2.4 2.1 1.1 2.0 0 1.4 0 2.6 4.1 3.5 5.2 5.8 6.2

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 4.6839 5.5275 4.8746 4.3241 4.2642 5.0454 2.8051 3.582 3.0758 2.5809 3.4953 3.7933 3.1172 4.144 3.6248 3.4186 3.0742 3.8778
Actin 0.0244 0.0087 0.0287 0.0204 0.0206 0.0113 0.0283 0.0029 0.0103 0.0003 0.0001 0.0056 0.0304 0.0023 0.0026 0.0167 0.0414 0.011
Bud 0.0004 0.0003 0.0002 0.0002 0.0002 0.0001 0.001 0.0003 0.0005 0 0 0.0002 0.0004 0.0002 0.0001 0.0004 0.0006 0.0002
Bud Neck 0.0004 0.0012 0.0005 0.0018 0.0003 0.0004 0.0011 0.0004 0.0006 0.0002 0.0001 0.0006 0.0009 0.0003 0.0002 0.0003 0.0005 0.0007
Bud Periphery 0.0007 0.0005 0.0002 0.0002 0.0002 0.0001 0.0013 0.0002 0.0007 0 0 0.0002 0.0004 0.0002 0.0001 0.0005 0.0015 0.0003
Bud Site 0.0011 0.0037 0.0011 0.0008 0.0004 0.0002 0.0067 0.0041 0.0025 0.0001 0 0.0002 0.0015 0.0006 0.0003 0.0013 0.0017 0.0004
Cell Periphery 0.0002 0.0003 0.0001 0.0001 0.0001 0 0.0004 0.0001 0.0006 0 0 0.0001 0.0001 0 0 0.0001 0.0002 0.0001
Cytoplasm 0.0113 0.0027 0.0028 0.0018 0.0021 0.0012 0.0027 0.0084 0.0064 0.0009 0.0009 0.0034 0.0065 0.0027 0.0018 0.001 0.0007 0.0011
Cytoplasmic Foci 0.129 0.1851 0.1541 0.1495 0.1458 0.1264 0.1125 0.1913 0.1903 0.1858 0.1233 0.1068 0.1523 0.1322 0.1258 0.0969 0.0992 0.1008
Eisosomes 0.0003 0 0.0001 0.0007 0.0002 0 0.0003 0 0.0029 0 0 0 0.0002 0 0 0.0003 0.0003 0.0001
Endoplasmic Reticulum 0.0042 0.0003 0.0005 0.0005 0.0013 0.0007 0.0027 0.0006 0.0016 0.0001 0.0003 0.0007 0.0021 0.0004 0.0009 0.0002 0.0008 0.0015
Endosome 0.278 0.2868 0.2024 0.3175 0.2521 0.1898 0.4293 0.4212 0.382 0.3479 0.4544 0.2621 0.2884 0.3276 0.2913 0.3363 0.1941 0.1937
Golgi 0.3666 0.4462 0.4153 0.3662 0.4801 0.5395 0.1761 0.3016 0.306 0.3904 0.3998 0.504 0.3452 0.4468 0.469 0.3943 0.471 0.6009
Lipid Particles 0.0532 0.0144 0.032 0.035 0.0244 0.0266 0.1471 0.0215 0.0308 0.0349 0.0084 0.0545 0.078 0.0349 0.0295 0.016 0.043 0.0275
Mitochondria 0.0242 0.0154 0.0442 0.0372 0.0151 0.025 0.0274 0.0144 0.0241 0.0038 0.0059 0.0231 0.0132 0.0246 0.033 0.0713 0.0964 0.0221
None 0.0075 0.0002 0.0003 0.001 0.0005 0.0002 0.0009 0.0002 0.0004 0 0 0.0001 0.0084 0.0001 0.0002 0.0002 0.0004 0.0002
Nuclear Periphery 0.0151 0.0001 0.0058 0.0002 0.0006 0.0027 0.0109 0.0003 0.0007 0 0.0001 0.0002 0.0024 0.0002 0.0002 0.0002 0.0014 0.0042
Nucleolus 0.0003 0.0002 0.0018 0.0002 0 0 0.0007 0.0001 0.0001 0 0 0.0001 0.0007 0.0001 0.0001 0.0001 0.0008 0.0002
Nucleus 0.0023 0.0002 0.0081 0.0001 0.0001 0.0001 0.0015 0.0001 0.0002 0 0 0.0001 0.0006 0.0001 0 0.0001 0.0014 0.0002
Peroxisomes 0.0677 0.0286 0.0915 0.0606 0.0521 0.0728 0.023 0.0243 0.034 0.0329 0.0022 0.0336 0.0525 0.0182 0.0404 0.0601 0.0253 0.0294
Punctate Nuclear 0.0034 0.0001 0.0032 0.0014 0.0017 0.0004 0.008 0.0006 0.0006 0.0002 0.0001 0.0003 0.0084 0.0002 0.0004 0.0006 0.0004 0.0006
Vacuole 0.0057 0.0029 0.0051 0.0021 0.0009 0.001 0.0115 0.0046 0.0026 0.0011 0.002 0.0018 0.0046 0.0055 0.0018 0.0014 0.0073 0.002
Vacuole Periphery 0.004 0.0019 0.002 0.0027 0.0012 0.0014 0.0066 0.0029 0.0021 0.0011 0.0024 0.0025 0.003 0.0029 0.0021 0.0016 0.0117 0.0027

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 3.9882 7.1304 22.928 23.4491 8.0436 3.5431 20.6274 21.0859 27.6578 15.9732
Translational Efficiency 1.6692 1.73 0.8842 1.0441 0.8333 2.7966 0.9594 0.9909 0.9799 1.0375

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✔
Translational Efficiency ✘
ORF
Human Ortholog
Description Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern; YMR253C is not an essential gene
Localization
Cell Percentages Golgi (7%)
Cell Cycle Regulation No
Subcompartmental Group N/A

YMR253C

YMR253C


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Ymr253c-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available