Standard name
Human Ortholog
Description Regulatory subunit of trehalose-6-phosphate synthase/phosphatase; involved in synthesis of storage carbohydrate trehalose; expression is induced by stress conditions and repressed by the Ras-cAMP pathway; TPS3 has a paralog, TSL1, that arose from the whole genome duplication
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0.07 0 0 0 0 0 0.07 – 0.08 0.08 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.99 0.96 0.96 0.98 0.93 0.89 0.79 0.72 0.66 – 0.64 0.61 0.98 0.99 0.96 0.89 0.88 0.9 0.99 0.97 0.95 0.91 0.89 0.88
Endoplasmic Reticulum 0 0 0 0 0.05 0 0.1 0.08 0.09 – 0.08 0.08 0 0 0 0 0.05 0.06 0 0 0 0 0 0.07
Endosome 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0.05 0 0.13 0.13 0.13 0.25 – 0.25 0.3 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0.1 0 – 0 0.08 0 0 0 0.1 0.08 0.09 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 5 6 10 8 – 10 13 0 0 2 0 0 0 0 0 0 0 0 0
Bud 0 1 0 0 0 1 1 5 2 – 4 5 0 3 2 0 0 1 0 0 0 0 2 2
Bud Neck 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 2 4 13 4 11 14 20 21 23 – 21 28 10 8 20 15 9 13 0 0 0 0 0 0
Cytoplasm 93 220 177 206 364 294 357 347 208 – 160 215 262 435 453 367 228 361 91 223 191 177 331 410
Endoplasmic Reticulum 0 6 3 1 19 12 46 38 27 – 20 28 1 3 9 13 12 22 0 2 1 8 16 33
Endosome 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 2 0 0 0 0 0 1 0 0
Golgi 0 0 0 0 0 0 0 0 1 – 0 1 0 0 0 3 4 3 0 0 0 1 0 0
Mitochondria 0 2 2 11 13 43 60 62 80 – 62 104 0 0 2 4 2 3 0 1 1 0 1 3
Nucleus 0 1 0 0 1 3 1 1 0 – 1 2 1 1 1 3 3 3 0 0 0 2 2 0
Nuclear Periphery 1 0 0 0 0 0 0 0 0 – 0 4 0 0 0 0 0 0 0 0 0 0 1 0
Nucleolus 0 0 0 0 0 0 0 0 0 – 0 0 0 0 1 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 1 1 0 0 1 6 4 48 10 – 7 27 1 3 2 42 21 37 0 1 3 2 10 4
Unique Cell Count 94 228 185 211 390 331 450 485 315 250 352 266 439 470 414 260 399 93 231 202 196 371 464
Labelled Cell Count 97 235 195 222 409 378 495 532 359 285 427 275 453 492 449 279 443 93 231 202 196 371 464


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 13.1 11.3 8.4 7.9 9.5 9.9 10.4 10.9 10.6 – 10.9 11.1 14.2 15.6 15.2 17.7 19.6 19.4 12.3 11.4 13.3
Std Deviation (1e-4) 2.0 1.8 1.2 1.2 1.6 1.8 2.1 2.4 2.6 – 3.1 3.4 2.6 3.0 3.5 5.8 5.4 6.4 2.1 3.1 2.9
Intensity Change (Log2) – – – -0.08 0.18 0.24 0.31 0.38 0.34 – 0.39 0.41 0.77 0.9 0.86 1.09 1.23 1.22 0.56 0.45 0.67


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Cell Periphery -2.5 -2.4 -1.4 -1.3 -1.4 0.1 – 0.5 0.4 -1.6 -3.3 -1.5 -1.8 -1.7 -2.1
Cytoplasm 1.1 -1.1 -2.6 -5.1 -6.8 -7.6 – -7.8 -8.6 1.8 2.8 0.4 -2.8 -2.9 -2.2
Endoplasmic Reticulum 0 1.9 1.3 3.7 3.0 3.2 – 2.9 3.0 0 0 0 0 1.7 2.2
Endosome 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Mitochondria 2.3 0 4.6 4.7 4.6 7.1 – 6.9 7.9 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 4.4 0 – 0 3.9 0 0 0 4.5 4.0 4.3

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 8.4661 12.9568 11.5458 9.2203 9.8503 11.3515 13.1853 17.6001 15.269 14.0118 14.5128 16.0593 10.5261 12.9852 11.4762 11.0529 10.2325 11.206
Actin 0.0557 0.0009 0.0069 0.0126 0.0015 0.0038 0.0137 0.0002 0.0105 0.0001 0.0031 0.0008 0.002 0.0005 0.0024 0.0002 0.0011 0.0018
Bud 0.0009 0.0007 0.0004 0.0018 0.0003 0.0004 0.0012 0.0002 0.0019 0.0004 0.0009 0.0004 0.0003 0.0005 0.0003 0.0005 0.0006 0.0007
Bud Neck 0.0061 0.0004 0.0005 0.0011 0.0005 0.0023 0.002 0.0001 0.0008 0.0005 0.0005 0.0011 0.0004 0.0001 0.0004 0.0004 0.0006 0.0008
Bud Periphery 0.0014 0.0003 0.0002 0.0007 0.0004 0.0005 0.0012 0 0.0011 0 0.0011 0.0005 0.0002 0.0001 0.0002 0.0001 0.0003 0.0037
Bud Site 0.0064 0.0021 0.0014 0.0016 0.0002 0.0002 0.0053 0.0003 0.0156 0.0002 0.0017 0.0001 0.0006 0.0006 0.0005 0.0001 0.0002 0.0001
Cell Periphery 0.0024 0.0004 0.0003 0.0002 0.0002 0.0003 0.0004 0.0001 0.0003 0 0.0002 0.0002 0.0003 0 0.0006 0 0.0001 0.0001
Cytoplasm 0.6678 0.9566 0.9053 0.8234 0.8931 0.8821 0.7645 0.987 0.861 0.9619 0.7452 0.9194 0.8543 0.9928 0.9589 0.8482 0.8886 0.9036
Cytoplasmic Foci 0.0297 0.0051 0.0099 0.0194 0.0073 0.009 0.0267 0.0022 0.0164 0.0076 0.0134 0.0078 0.0174 0.0019 0.0068 0.0151 0.0163 0.012
Eisosomes 0.0005 0 0 0.0001 0 0.0002 0.0003 0 0.0001 0 0.0001 0 0 0 0.0005 0 0 0
Endoplasmic Reticulum 0.0466 0.0096 0.0118 0.0074 0.0301 0.047 0.0474 0.0039 0.0126 0.0054 0.0043 0.0311 0.0395 0.0006 0.0042 0.005 0.0224 0.0222
Endosome 0.0674 0.0107 0.0256 0.0435 0.0328 0.0237 0.0399 0.0024 0.0331 0.0131 0.1122 0.0209 0.045 0.0011 0.0101 0.071 0.0482 0.0332
Golgi 0.0212 0.0016 0.0049 0.0109 0.0035 0.0087 0.0058 0.0002 0.0095 0.0004 0.0283 0.0023 0.0083 0.0002 0.004 0.0019 0.0038 0.0038
Lipid Particles 0.0099 0.0002 0.007 0.0255 0.0014 0.0002 0.0055 0 0.002 0.0001 0.0044 0.0004 0.0007 0 0.0004 0 0.0009 0.0005
Mitochondria 0.0134 0.0027 0.0013 0.0031 0.009 0.0121 0.0258 0.0002 0.0177 0.0002 0.0514 0.0025 0.0019 0.0001 0.0051 0.0004 0.0034 0.0056
None 0.0193 0.0007 0.001 0.0008 0.001 0.0006 0.0029 0.0003 0.0006 0.0004 0.0005 0.0005 0.0018 0.0004 0.0007 0.0005 0.0008 0.0034
Nuclear Periphery 0.0124 0.0013 0.0054 0.0022 0.0077 0.0021 0.0191 0.0004 0.0027 0.001 0.0027 0.0044 0.0048 0.0001 0.0006 0.002 0.0025 0.0025
Nucleolus 0.0006 0.0001 0.0001 0.0001 0.0001 0 0.0012 0 0.0002 0 0.0002 0 0 0 0 0 0 0.0001
Nucleus 0.0087 0.0016 0.0042 0.0019 0.0016 0.0012 0.0125 0.0007 0.0019 0.0016 0.0016 0.0016 0.0016 0.0003 0.0006 0.001 0.0011 0.0012
Peroxisomes 0.0078 0.0001 0.004 0.0249 0.0001 0.0001 0.0046 0 0.002 0.0001 0.0013 0.0002 0.0004 0 0.0001 0.0001 0.0001 0.0001
Punctate Nuclear 0.0074 0.0002 0.0006 0.0015 0.0001 0.0001 0.0037 0 0.0018 0.0001 0.0003 0.0003 0.0002 0 0.0001 0.0001 0.0003 0.0012
Vacuole 0.0118 0.0039 0.0076 0.0137 0.0063 0.0037 0.0122 0.0016 0.0067 0.0057 0.0159 0.004 0.0173 0.0006 0.0027 0.0379 0.0059 0.0022
Vacuole Periphery 0.0027 0.0008 0.0017 0.0039 0.0026 0.0016 0.004 0.0002 0.0015 0.0011 0.0104 0.0015 0.0029 0.0001 0.0007 0.0154 0.0025 0.0012

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 47.4133 31.1651 30.5261 57.9283 46.557 46.3417 37.9708 53.7012 65.0 61.8134
Translational Efficiency 1.2546 1.1144 1.4749 1.1239 1.0849 1.5304 1.1791 0.9727 1.1314 1.0687

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✔
Translational Efficiency ✘
Standard name
Human Ortholog
Description Regulatory subunit of trehalose-6-phosphate synthase/phosphatase; involved in synthesis of storage carbohydrate trehalose; expression is induced by stress conditions and repressed by the Ras-cAMP pathway; TPS3 has a paralog, TSL1, that arose from the whole genome duplication
Localization
Cell Percentages cytoplasm (100%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-7

Tps3

Tps3


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Tps3-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available