Standard name | |
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Human Ortholog | |
Description | Splicing factor that reanneals snRNPs during spliceosome recycling; reanneals U4 and U6 snRNPs |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 1 | 0 | 0 | 0 | 1 | 6 | 16 | 9 | 13 | 14 | 12 | 12 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 0 | 1 | 0 | 1 | 2 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 |
Bud Site | 0 | 0 | 0 | 1 | 5 | 7 | 11 | 19 | 15 | 26 | 27 | 17 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 3 | 7 | 18 | 18 | 28 | 32 | 46 | 38 | 22 | 34 | 19 | 18 | 21 | 48 | 58 | 10 | 20 | 17 | 1 | 0 | 3 | 4 | 4 | 6 |
Endoplasmic Reticulum | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 1 | 4 | 10 | 5 | 0 | 0 | 0 | 1 | 1 | 1 |
Endosome | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 3 | 0 | 0 | 0 | 0 | 1 | 3 | 8 | 1 | 2 | 1 | 0 | 1 | 2 | 1 | 0 | 3 |
Golgi | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 2 | 0 | 2 | 0 | 0 | 1 |
Mitochondria | 51 | 23 | 28 | 81 | 106 | 248 | 440 | 375 | 340 | 456 | 383 | 454 | 3 | 3 | 3 | 6 | 11 | 6 | 0 | 2 | 3 | 1 | 1 | 6 |
Nucleus | 150 | 124 | 281 | 241 | 254 | 282 | 444 | 500 | 431 | 497 | 407 | 529 | 133 | 206 | 219 | 105 | 145 | 134 | 146 | 121 | 271 | 86 | 187 | 152 |
Nuclear Periphery | 0 | 1 | 1 | 1 | 1 | 1 | 4 | 3 | 2 | 4 | 5 | 6 | 0 | 1 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 5 | 10 | 6 | 10 | 12 | 17 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 0 | 1 | 4 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 1 | 1 | 2 | 2 | 2 | 3 | 3 | 2 | 0 | 0 | 2 | 1 | 0 | 1 | 0 | 2 | 5 | 0 | 1 | 3 |
Vac/Vac Membrane | 2 | 7 | 14 | 8 | 24 | 14 | 13 | 21 | 7 | 14 | 18 | 28 | 2 | 10 | 13 | 10 | 25 | 24 | 10 | 8 | 38 | 14 | 35 | 52 |
Unique Cell Count | 166 | 137 | 323 | 280 | 348 | 423 | 650 | 695 | 561 | 678 | 554 | 719 | 158 | 267 | 294 | 127 | 189 | 177 | 166 | 140 | 337 | 116 | 242 | 240 |
Labelled Cell Count | 207 | 162 | 345 | 351 | 421 | 591 | 982 | 980 | 838 | 1060 | 886 | 1083 | 160 | 272 | 306 | 139 | 213 | 190 | 166 | 140 | 337 | 116 | 242 | 240 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 4.1 | 4.7 | 4.7 | 4.3 | 4.3 | 3.7 | 3.6 | 3.6 | 3.5 | 3.4 | 3.2 | 3.3 | 6.0 | 5.8 | 5.6 | 6.6 | 6.6 | 6.8 | 5.6 | 6.3 | 6.7 |
Std Deviation (1e-4) | 0.6 | 0.9 | 1.0 | 1.4 | 1.1 | 1.3 | 1.4 | 1.2 | 1.1 | 1.4 | 1.1 | 0.6 | 1.5 | 1.0 | 1.0 | 1.2 | 1.4 | 1.7 | 1.2 | 1.4 | 1.7 |
Intensity Change (Log2) | – | – | – | -0.13 | -0.15 | -0.35 | -0.4 | -0.41 | -0.45 | -0.48 | -0.56 | -0.52 | 0.33 | 0.28 | 0.24 | 0.48 | 0.49 | 0.51 | 0.25 | 0.41 | 0.51 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Actin | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Bud Neck | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Bud Site | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Cell Periphery | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Cyto | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endoplasmic Reticulum | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Endosome | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Golgi | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Mitochondria | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Nuclear Periphery | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Nuc | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleolus | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Peroxisomes | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
SpindlePole | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Vac | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cortical Patches | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Cytoplasm | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Nucleus | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Vacuole | – | – | – | – | – | – | – | – | – | – | – | – | 1.5 | 3.7 | 3.7 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 2.2192 | 1.9627 | 2.1665 | 2.375 | 1.64 | 1.9572 | -0.0686 | 0.2438 | -0.006 | -0.0989 | -0.493 | 0.0355 | -0.1797 | 0.1656 | -0.3027 | 0.321 | -0.6197 | -0.2256 |
Actin | 0.0274 | 0.0001 | 0.0003 | 0.0002 | 0.0001 | 0 | 0.0011 | 0.0002 | 0.0005 | 0.0068 | 0 | 0.0002 | 0.0237 | 0.0014 | 0.0027 | 0.0027 | 0.0099 | 0.0002 |
Bud | 0.0013 | 0.0001 | 0.0001 | 0 | 0 | 0 | 0.0003 | 0.0001 | 0.0001 | 0.0018 | 0 | 0.0002 | 0.0007 | 0.0001 | 0.0003 | 0.0005 | 0.0004 | 0 |
Bud Neck | 0.0028 | 0.0004 | 0.0008 | 0.0002 | 0.0005 | 0.0018 | 0.0002 | 0.0001 | 0.0001 | 0.0007 | 0.0001 | 0.0015 | 0.0029 | 0.0001 | 0.0004 | 0.0027 | 0.0008 | 0.0008 |
Bud Periphery | 0.0061 | 0 | 0.0001 | 0 | 0 | 0 | 0.0004 | 0.0001 | 0.0001 | 0.0012 | 0 | 0.0008 | 0.0018 | 0 | 0.0004 | 0.0014 | 0.001 | 0.0001 |
Bud Site | 0.0019 | 0.0004 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0006 | 0.0002 | 0.0001 | 0.0006 | 0 | 0.0001 | 0.0016 | 0.0001 | 0.003 | 0.0013 | 0.0015 | 0.0001 |
Cell Periphery | 0.0035 | 0 | 0 | 0 | 0 | 0 | 0.0001 | 0 | 0 | 0.0001 | 0 | 0.0001 | 0.0005 | 0 | 0.0001 | 0.0008 | 0.0001 | 0 |
Cytoplasm | 0.0175 | 0.042 | 0.0086 | 0.0154 | 0.0058 | 0.0066 | 0.0067 | 0.0104 | 0.0167 | 0.0333 | 0.0004 | 0.001 | 0.006 | 0.0051 | 0.0031 | 0.0015 | 0.0043 | 0.0017 |
Cytoplasmic Foci | 0.0008 | 0.0002 | 0.0002 | 0.0001 | 0 | 0.0007 | 0.0577 | 0.0002 | 0.0096 | 0.0019 | 0 | 0 | 0.007 | 0.0016 | 0.0068 | 0.0016 | 0.0054 | 0.0001 |
Eisosomes | 0.0004 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0.0002 | 0 | 0.0001 | 0.0001 | 0.0002 | 0 |
Endoplasmic Reticulum | 0.012 | 0.0011 | 0.0004 | 0.0007 | 0.0007 | 0.0003 | 0.0033 | 0.0006 | 0.0005 | 0.0051 | 0 | 0.0005 | 0.0033 | 0.0009 | 0.0008 | 0.0022 | 0.0032 | 0.0002 |
Endosome | 0.0094 | 0.0006 | 0.0005 | 0.0005 | 0.0005 | 0.0003 | 0.0277 | 0.0005 | 0.0085 | 0.0102 | 0 | 0.0014 | 0.0196 | 0.0011 | 0.0079 | 0.0204 | 0.0266 | 0.0026 |
Golgi | 0.0017 | 0 | 0 | 0 | 0 | 0 | 0.0042 | 0 | 0.0011 | 0.0007 | 0 | 0 | 0.0054 | 0.0001 | 0.0019 | 0.0056 | 0.0053 | 0.0019 |
Lipid Particles | 0.001 | 0 | 0.0001 | 0 | 0 | 0.0001 | 0.0119 | 0 | 0.0019 | 0.0002 | 0 | 0 | 0.021 | 0.0003 | 0.0061 | 0.0068 | 0.0303 | 0.0001 |
Mitochondria | 0.0098 | 0.0003 | 0.001 | 0.0008 | 0.0004 | 0.0001 | 0.0044 | 0.0015 | 0.0162 | 0.0036 | 0.0002 | 0.003 | 0.0047 | 0.0008 | 0.0056 | 0.0687 | 0.0121 | 0.0068 |
None | 0.0099 | 0.0035 | 0.0429 | 0.0196 | 0.0015 | 0.0157 | 0.0014 | 0.0015 | 0.0043 | 0.0012 | 0.0001 | 0.0001 | 0.0051 | 0.0042 | 0.0015 | 0.0006 | 0.0021 | 0.0001 |
Nuclear Periphery | 0.037 | 0.0061 | 0.0092 | 0.0157 | 0.0271 | 0.0024 | 0.0315 | 0.0133 | 0.0129 | 0.0532 | 0.0036 | 0.0078 | 0.0137 | 0.0189 | 0.0129 | 0.0122 | 0.0304 | 0.0017 |
Nucleolus | 0.0195 | 0.0043 | 0.0082 | 0.0071 | 0.0071 | 0.0097 | 0.0047 | 0.0032 | 0.0034 | 0.0019 | 0.0043 | 0.008 | 0.0183 | 0.0045 | 0.0065 | 0.0046 | 0.007 | 0.0075 |
Nucleus | 0.8187 | 0.9372 | 0.9202 | 0.9247 | 0.9522 | 0.9353 | 0.8029 | 0.9537 | 0.918 | 0.8691 | 0.9909 | 0.9685 | 0.8404 | 0.9537 | 0.9151 | 0.8385 | 0.8358 | 0.9736 |
Peroxisomes | 0.0005 | 0 | 0 | 0 | 0 | 0 | 0.0124 | 0 | 0.0009 | 0.0006 | 0 | 0 | 0.0068 | 0.0002 | 0.0183 | 0.0015 | 0.0081 | 0 |
Punctate Nuclear | 0.0096 | 0.0026 | 0.0064 | 0.0141 | 0.0031 | 0.0261 | 0.018 | 0.0135 | 0.004 | 0.0023 | 0.0002 | 0.001 | 0.0092 | 0.0063 | 0.0052 | 0.002 | 0.0118 | 0.0019 |
Vacuole | 0.006 | 0.001 | 0.0006 | 0.0005 | 0.0005 | 0.0004 | 0.0056 | 0.0005 | 0.0005 | 0.0031 | 0.0001 | 0.0029 | 0.0061 | 0.0003 | 0.001 | 0.0145 | 0.0017 | 0.0002 |
Vacuole Periphery | 0.0034 | 0.0001 | 0.0004 | 0.0003 | 0.0003 | 0.0001 | 0.0049 | 0.0004 | 0.0005 | 0.0025 | 0.0001 | 0.0027 | 0.0022 | 0.0003 | 0.0004 | 0.0098 | 0.0019 | 0.0003 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 7.8519 | 2.2166 | 5.4826 | 6.4974 | 10.7162 | 3.3043 | 6.1632 | 4.2858 | 5.1323 | 7.6898 |
Translational Efficiency | 1.3156 | 3.1565 | 1.1408 | 0.8871 | 0.6612 | 3.4245 | 1.6816 | 1.7348 | 1.5809 | 1.1351 |
Dataset | Hit |
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Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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