Standard name
Human Ortholog
Description Phosphoribomutase; catalyzes interconversion of ribose-1-phosphate and ribose-5-phosphate; has some phosphoglucomutase activity but primary activity in vivo is phosphoribomutase; contributes to ribose recycling in the pentose phosphate pathway; transcription induced in response to stress; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; non-essential
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.99 0.98 1.0 0.97 0.97 0.95 0.92 – 0.84 0.8 0.7 0.82 0.99 1.0 1.0 0.94 0.95 0.94 0.91 0.9 0.91 0.92 0.87 0.87
Endoplasmic Reticulum 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0.05 – 0.15 0.12 0.39 0.19 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 – 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 – 0 0.09 0 0 0 0 0 0.05 0 0.06 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 1 – 0 0 0 2 1 0 0 0 0 0 0 0 0 0 1 0
Bud 0 0 0 0 0 0 2 – 2 3 3 1 1 0 0 0 0 0 2 8 2 0 5 10
Bud Neck 0 0 0 1 3 3 4 – 3 5 1 4 0 0 0 0 0 0 0 0 0 0 0 1
Bud Site 0 0 0 0 0 0 0 – 1 0 0 1 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 2 1 2 2 1 2 – 2 7 1 3 3 1 0 4 7 3 0 0 0 0 0 0
Cytoplasm 162 173 36 199 366 309 436 – 228 356 162 270 118 214 214 89 156 147 150 165 69 40 176 251
Endoplasmic Reticulum 1 2 0 0 0 3 2 – 1 4 2 7 0 0 0 6 2 1 0 0 0 0 1 2
Endosome 0 0 0 0 0 0 0 – 0 1 0 0 0 0 0 0 0 0 2 4 0 0 1 0
Golgi 0 1 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0
Mitochondria 4 2 0 1 2 13 22 – 41 52 90 64 0 0 0 2 0 1 3 0 1 0 1 4
Nucleus 0 0 0 2 4 3 10 – 9 9 11 7 1 0 1 1 2 2 0 0 0 0 0 1
Nuclear Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 1 0 0 1 – 2 2 2 5 0 0 0 0 0 0 0 0 0 0 1 5
Vac/Vac Membrane 0 1 0 5 10 8 14 – 9 41 10 13 0 0 1 5 7 10 1 0 1 1 8 2
Unique Cell Count 164 177 36 205 379 326 476 273 444 233 330 119 214 215 95 164 156 166 184 76 44 202 289
Labelled Cell Count 167 181 37 211 387 340 494 298 480 282 377 124 215 216 107 174 164 166 184 76 44 202 289


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.3 7.5 7.8 6.9 7.0 5.7 6.0 – 5.1 5.6 4.5 5.0 6.6 6.5 6.1 22.4 24.4 22.7 – 6.6 6.8
Std Deviation (1e-4) 2.0 3.2 1.9 2.0 2.1 2.1 2.2 – 2.0 2.4 1.7 2.0 1.8 2.1 1.9 6.9 6.3 7.3 – 2.2 2.3
Intensity Change (Log2) – – – -0.18 -0.16 -0.44 -0.37 – -0.62 -0.48 -0.79 -0.63 -0.24 -0.26 -0.34 1.53 1.65 1.55 – -0.25 -0.18


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Cytoplasm -1.0 -1.1 -1.4 -1.8 – -2.6 -3.0 -3.9 -2.8 -0.6 0 -0.4 -1.5 -1.4 -1.5
Endoplasmic Reticulum 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 – 0 0 0 2.9 0 0 0 0 0 0
Nucleus 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 1.0076 1.6045 0.6878 1.0984 0.8507 1.1852 2.0256 2.8512 2.7886 2.4941 1.3349 3.7621 0.1313 0.9635 0.7109 1.01 -0.1559 1.1895
Actin 0.0846 0.0002 0.02 0.0099 0.0539 0.061 0.0608 0.0133 0.0126 0.0034 0.0002 0.0011 0.0545 0.0201 0.0466 0.0003 0.003 0.0002
Bud 0.0018 0.0001 0.016 0.0002 0.0017 0.0039 0.0013 0.0015 0.0004 0.0004 0.0001 0.0002 0.0006 0.0008 0.0096 0.0001 0.0002 0
Bud Neck 0.0022 0.0001 0.0003 0.0005 0.0012 0.0037 0.0152 0.0002 0.0001 0.0006 0.0004 0.0006 0.0005 0.0002 0.0005 0.0005 0.0004 0.0003
Bud Periphery 0.0027 0 0.0026 0.0003 0.0038 0.0073 0.0041 0.002 0.0004 0.0004 0 0.0002 0.0006 0.0008 0.0111 0 0.0004 0
Bud Site 0.018 0.0002 0.011 0.0007 0.002 0.0045 0.0183 0.005 0.0005 0.0003 0 0.0002 0.0059 0.0012 0.0136 0.0002 0.0009 0
Cell Periphery 0.0009 0 0.0002 0.0001 0.0007 0.0003 0.0034 0.0002 0.0001 0.0001 0 0 0.0002 0.0002 0.0005 0 0.0002 0
Cytoplasm 0.3444 0.7265 0.5903 0.3512 0.4798 0.5872 0.4126 0.7076 0.7207 0.755 0.8937 0.7428 0.4238 0.6384 0.4595 0.0103 0.5007 0.6388
Cytoplasmic Foci 0.0517 0.0018 0.0045 0.0296 0.0293 0.0089 0.0242 0.0106 0.0037 0.0048 0.0042 0.0038 0.08 0.0119 0.0076 0.3392 0.0115 0.0022
Eisosomes 0.001 0 0.0002 0.0002 0.0013 0.0002 0.0042 0.0002 0.0001 0 0 0 0.0003 0.0002 0.0002 0 0.0002 0
Endoplasmic Reticulum 0.0109 0.0023 0.0018 0.0003 0.031 0.004 0.0137 0.0012 0.0027 0.0009 0.0033 0.002 0.0068 0.0027 0.0027 0.0001 0.0043 0.0007
Endosome 0.045 0.0006 0.003 0.0191 0.1373 0.0056 0.0349 0.0036 0.019 0.0145 0.0019 0.0061 0.0612 0.0114 0.021 0.002 0.024 0.0019
Golgi 0.0287 0 0.0049 0.0509 0.0247 0.0062 0.0255 0.0026 0.0035 0.0234 0.0001 0.0084 0.0166 0.0177 0.0091 0.0017 0.0193 0.0017
Lipid Particles 0.0596 0.0001 0.0008 0.1325 0.0275 0.0014 0.0154 0.0016 0.0004 0.0008 0 0.0036 0.0697 0.0029 0.0007 0.001 0.0253 0.0064
Mitochondria 0.0433 0.0001 0.0055 0.0579 0.0497 0.0119 0.0169 0.0045 0.0026 0.0198 0.0001 0.0109 0.0131 0.0073 0.0093 0.0001 0.2433 0.034
None 0.2137 0.253 0.3322 0.3134 0.0632 0.2862 0.3147 0.2415 0.2274 0.1451 0.09 0.1824 0.1749 0.2732 0.392 0.4919 0.1502 0.3079
Nuclear Periphery 0.0343 0.0012 0.0007 0.0002 0.0449 0.0014 0.0151 0.0003 0.001 0.0008 0.0012 0.0262 0.0126 0.0013 0.0013 0.0001 0.0008 0.0005
Nucleolus 0.0017 0 0.0002 0.0002 0.002 0.0001 0.0006 0.0001 0 0.0001 0 0.0001 0.0004 0.0001 0.0002 0.0002 0.0004 0
Nucleus 0.0139 0.0129 0.0028 0.0003 0.0053 0.0022 0.0022 0.001 0.0015 0.0272 0.0034 0.0039 0.0042 0.0014 0.0013 0.0004 0.001 0.0027
Peroxisomes 0.0252 0 0.0004 0.0302 0.0048 0.0023 0.0034 0.0015 0.0001 0.0005 0 0.0023 0.0555 0.0034 0.0019 0.0086 0.0082 0.0019
Punctate Nuclear 0.0068 0.0004 0.0014 0.0006 0.0084 0.0006 0.0008 0.0003 0.0003 0.0003 0.0007 0.0002 0.0048 0.0035 0.0004 0.1431 0.0005 0.0003
Vacuole 0.0054 0.0005 0.0009 0.0008 0.0132 0.0007 0.0067 0.001 0.0018 0.0009 0.0007 0.0013 0.0085 0.001 0.0075 0 0.003 0.0003
Vacuole Periphery 0.0041 0.0001 0.0002 0.0008 0.0143 0.0003 0.006 0.0002 0.001 0.0005 0.0001 0.0037 0.0052 0.0004 0.0033 0 0.0023 0.0002

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 35.7175 26.9156 21.539 30.9221 34.4449 23.0117 31.75 32.4138 27.3536 33.3331
Translational Efficiency 0.8038 1.0581 1.1562 0.9356 0.7231 1.0401 0.8365 0.689 0.9174 0.7846

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Phosphoribomutase; catalyzes interconversion of ribose-1-phosphate and ribose-5-phosphate; has some phosphoglucomutase activity but primary activity in vivo is phosphoribomutase; contributes to ribose recycling in the pentose phosphate pathway; transcription induced in response to stress; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; non-essential
Localization
Cell Percentages cytoplasm (38%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

Prm15

Prm15


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Prm15-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available