Standard name
Human Ortholog
Description Zinc cluster transcriptional activator; necessary for derepression of a variety of genes under non-fermentative growth conditions, active after diauxic shift, binds carbon source responsive elements; relative distribution to the nucleus increases upon DNA replication stress
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.78 0.91 – 1.0 0.92 0.84 0.67 0.74 0.56 0.68 0.53 0.53 0.96 0.97 0.92 0.92 0.95 0.96 0.51 0.84 0.83 0.8 0.82 0.81
Endoplasmic Reticulum 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0.09 0.05 0.08 0.12 0 0 0 0 0
Endosome 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0.17 – 0.13 0.17 0.21 0.42 0.4 0.61 0.54 0.51 0.65 0.05 0.06 0.11 0.07 0 0 0.14 0.07 0.07 0 0 0
Nucleus 0.17 0 – 0 0 0 0 0 0 0 0.05 0.08 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 – 0 0 0 0.05 0 0 0 0.05 0 0 0 0.05 0 0 0 0.09 0 0 0.06 0.06 0.05
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 1 0 0 0
Bud 0 2 – 0 1 2 2 2 0 3 0 3 0 0 5 0 0 0 3 0 0 0 1 4
Bud Neck 1 0 – 0 1 0 0 1 0 1 0 0 0 0 0 2 1 0 0 0 0 1 0 0
Bud Site 0 0 – 0 0 0 0 2 0 1 2 1 0 0 0 0 0 0 – – – – – –
Cell Periphery 1 0 – 0 0 0 0 0 0 0 1 0 0 0 0 2 2 5 0 1 0 0 0 0
Cytoplasm 79 132 – 23 109 64 96 134 53 77 43 55 106 235 271 94 131 230 126 123 24 73 120 163
Endoplasmic Reticulum 0 0 – 0 0 0 2 0 0 0 2 0 0 0 1 9 7 18 30 0 0 1 0 1
Endosome 3 0 – 0 0 0 0 0 0 0 0 0 0 2 0 0 1 1 9 1 0 0 2 2
Golgi 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 2 1 2 0 0 0 1 0 0
Mitochondria 0 25 – 3 20 16 60 73 58 61 41 67 5 14 33 7 3 5 34 10 1 3 3 8
Nucleus 17 0 – 0 2 0 4 7 3 5 4 8 1 0 1 1 0 3 4 1 0 0 1 1
Nuclear Periphery 1 2 – 0 0 1 1 0 0 0 0 0 0 0 1 1 0 0 1 0 0 0 0 0
Nucleolus 0 0 – 0 0 0 0 1 1 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0
Peroxisomes 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 – 0 0 0 1 2 1 0 1 0 0 0 0 0 1 0 4 0 0 0 1 4
Vac/Vac Membrane 2 4 – 0 1 1 7 2 2 2 4 2 2 4 14 3 2 2 23 6 0 5 8 9
Unique Cell Count 101 145 23 119 76 144 181 95 114 81 103 110 243 294 102 138 239 248 148 29 92 147 203
Labelled Cell Count 104 165 26 134 84 173 224 118 150 98 136 114 255 326 121 150 266 248 148 29 92 147 203


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.0 3.1 – 2.8 3.3 3.6 3.1 3.2 3.0 2.6 3.7 2.6 4.1 4.0 3.9 5.3 5.3 6.0 4.1 3.8 4.5
Std Deviation (1e-4) 0.7 0.8 – 0.6 1.6 2.0 1.3 2.0 1.9 1.1 2.3 1.3 0.8 1.1 1.2 1.8 2.0 2.3 1.1 1.2 1.5
Intensity Change (Log2) – – – – – – – – – – – – – – – – – – – – –


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – –
Bud Neck – – – – – – – – – – – – – – –
Bud Site – – – – – – – – – – – – – – –
Cell Periphery – – – – – – – – – – – – – – –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – –
Endosome – – – – – – – – – – – – – – –
Golgi – – – – – – – – – – – – – – –
Mitochondria – – – – – – – – – – – – – – –
Nuclear Periphery – – – – – – – – – – – – – – –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – –
Peroxisomes – – – – – – – – – – – – – – –
SpindlePole – – – – – – – – – – – – – – –
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – – –
Cytoplasm – – – – – – – – – – – – – – –
Nucleus – – – – – – – – – – – – – – –
Vacuole – – – – – – – – – – – – – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration -0.3431 0.0778 0.1786 -0.5214 -0.8335 -0.0497 0.5631 0.4045 0.4997 -0.1516 0.1248 0.5658 -1.4623 -1.7361 -1.4768 -1.303 -0.4748 -1.236
Actin 0.0246 0.0032 0.0357 0.0003 0.0629 0.0016 0.029 0.0028 0.0082 0.0006 0.0024 0.0083 0.0567 0.0003 0.0001 0.0246 0.0506 0.0013
Bud 0.0012 0.0005 0.0011 0.0002 0.0003 0.0001 0.001 0.0028 0.0009 0.0001 0.0005 0.0003 0.0021 0 0 0.0018 0.0006 0.0006
Bud Neck 0.0117 0.0006 0.0085 0.0003 0.0013 0.0019 0.0009 0.0001 0.0003 0.0002 0.0034 0.0018 0.0002 0 0 0.0013 0.001 0.0049
Bud Periphery 0.0018 0.0007 0.002 0.0004 0.0003 0.0001 0.0032 0.0027 0.0013 0.0001 0.0007 0.0005 0.0175 0 0 0.0025 0.0007 0.0019
Bud Site 0.0249 0.0221 0.0055 0.0003 0.0014 0.0003 0.0019 0.0049 0.0027 0.0001 0.0079 0.0005 0.0005 0.0001 0.0001 0.0061 0.0039 0.0002
Cell Periphery 0.0007 0.0004 0.0013 0.0001 0.0001 0.0001 0.0004 0.0001 0.0004 0 0.0003 0.0001 0.0004 0 0 0.0005 0 0.0001
Cytoplasm 0.152 0.287 0.1943 0.2805 0.1591 0.3222 0.1301 0.283 0.2307 0.2332 0.2885 0.316 0.0225 0.0887 0.0696 0.0275 0.0294 0.0928
Cytoplasmic Foci 0.0157 0.0068 0.0117 0.0087 0.0451 0.0162 0.0485 0.0134 0.0197 0.0122 0.0288 0.0175 0.0067 0.0026 0.0081 0.0059 0.0118 0.0097
Eisosomes 0.0004 0.0002 0.0008 0.0002 0.002 0.0001 0.0003 0 0.0001 0.0001 0.0001 0.0001 0.0007 0.0001 0.0002 0.0003 0.0002 0.0001
Endoplasmic Reticulum 0.0031 0.0026 0.0055 0.0032 0.0025 0.0029 0.0056 0.0014 0.0027 0.0012 0.0054 0.0021 0.0009 0.0015 0.0007 0.0018 0.0004 0.001
Endosome 0.0079 0.0041 0.0113 0.0042 0.0069 0.0045 0.0447 0.0016 0.0164 0.0077 0.0228 0.0089 0.0045 0.0005 0.0011 0.0278 0.0008 0.0007
Golgi 0.0076 0.0042 0.0082 0.0001 0.0028 0.0007 0.0184 0.0002 0.0075 0.0043 0.0129 0.008 0.0059 0.0001 0.0001 0.018 0.0032 0.0001
Lipid Particles 0.0128 0.0104 0.0096 0.0015 0.0072 0.001 0.032 0.0003 0.0092 0.0126 0.0325 0.0107 0.0122 0.0002 0.0003 0.002 0.0012 0.0003
Mitochondria 0.0024 0.0052 0.0196 0.0007 0.0019 0.021 0.0123 0.0003 0.004 0.001 0.0179 0.0061 0.0025 0.0001 0.0002 0.0141 0.0018 0.0002
None 0.7009 0.6178 0.6153 0.6771 0.6636 0.5629 0.6394 0.6682 0.6626 0.7074 0.5515 0.5809 0.8467 0.904 0.9033 0.857 0.8608 0.8793
Nuclear Periphery 0.0024 0.0012 0.009 0.002 0.0062 0.0029 0.0087 0.0012 0.0052 0.0011 0.0033 0.0039 0.0024 0.0003 0.0013 0.0016 0.0002 0.0006
Nucleolus 0.0007 0.0004 0.0015 0.0007 0.0029 0.001 0.0006 0.0002 0.0005 0.0001 0.001 0.0006 0.0011 0.0001 0.0003 0.0002 0 0.0002
Nucleus 0.0152 0.0206 0.0474 0.0113 0.0107 0.0441 0.0033 0.0084 0.0098 0.0065 0.0024 0.0145 0.0008 0.0008 0.0102 0.0007 0.0001 0.0012
Peroxisomes 0.0079 0.0086 0.0055 0.0005 0.0036 0.0007 0.0099 0.0009 0.0066 0.0076 0.0051 0.0064 0.0045 0.0001 0.0001 0.0015 0.032 0.0002
Punctate Nuclear 0.0025 0.0015 0.0021 0.0016 0.0181 0.0143 0.003 0.0063 0.0079 0.0029 0.0061 0.0109 0.0106 0.0003 0.0037 0.0005 0.0009 0.0044
Vacuole 0.0034 0.0016 0.0032 0.0056 0.0006 0.0011 0.0042 0.001 0.0025 0.0008 0.0054 0.0012 0.0003 0.0004 0.0004 0.0028 0.0001 0.0003
Vacuole Periphery 0.0004 0.0002 0.0009 0.0005 0.0005 0.0002 0.0027 0.0001 0.0009 0.0002 0.0013 0.0009 0.0003 0 0.0001 0.0013 0 0.0001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 0.513 0.2293 0.5104 1.1867 0.4988 0.769 1.1591 1.2517 1.3476 1.35
Translational Efficiency – – – – – – – – – –

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency –
Standard name
Human Ortholog
Description Zinc cluster transcriptional activator; necessary for derepression of a variety of genes under non-fermentative growth conditions, active after diauxic shift, binds carbon source responsive elements; relative distribution to the nucleus increases upon DNA replication stress
Localization
Cell Percentages nucleus (35%), cytoplasm (18%), mixed (22%)
Cell Cycle Regulation No
Subcompartmental Group N/A

Cat8

Cat8


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Cat8-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available