Standard name | |
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Human Ortholog | |
Description | ER membrane protein involved in the second step of GPI anchor assembly; the second step is the de-N-acetylation of the N-acetylglucosaminylphosphatidylinositol intermediate; functional homolog of human PIG-Lp; GPI stands for glycosylphosphatidylinositol |
ensLOC | DeepLoc | |||||||||||||||||||||||
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Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 9 | 6 | 16 | 9 | 4 | 12 | 17 | 15 | 15 | 16 | 17 | 15 | 6 | 7 | 8 | 15 | 16 | 16 | 73 | 89 | 52 | 31 | 60 | 73 |
Bud | 5 | 6 | 1 | 3 | 5 | 6 | 6 | 11 | 14 | 14 | 12 | 16 | 3 | 1 | 1 | 6 | 4 | 1 | 3 | 4 | 0 | 2 | 4 | 8 |
Bud Neck | 0 | 13 | 7 | 0 | 2 | 1 | 2 | 1 | 0 | 0 | 1 | 0 | 2 | 3 | 5 | 1 | 1 | 0 | 17 | 29 | 8 | 6 | 14 | 15 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 4 | 19 | 13 | 5 | 2 | 15 | 13 | 9 | 13 | 18 | 11 | 16 | 10 | 25 | 34 | 20 | 12 | 14 | 21 | 55 | 27 | 24 | 40 | 63 |
Cytoplasm | 265 | 543 | 190 | 111 | 92 | 154 | 146 | 173 | 118 | 104 | 69 | 93 | 225 | 345 | 382 | 162 | 61 | 64 | 63 | 221 | 30 | 75 | 113 | 92 |
Endoplasmic Reticulum | 11 | 22 | 9 | 11 | 4 | 25 | 29 | 19 | 25 | 26 | 28 | 39 | 7 | 5 | 3 | 17 | 9 | 9 | 71 | 96 | 48 | 26 | 55 | 82 |
Endosome | 1 | 0 | 0 | 0 | 1 | 0 | 1 | 1 | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 1 | 3 | 1 | 8 | 11 | 2 | 4 | 5 | 5 |
Golgi | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 2 | 0 | 1 | 1 | 1 |
Mitochondria | 6 | 11 | 0 | 1 | 6 | 14 | 23 | 30 | 91 | 66 | 51 | 68 | 0 | 0 | 0 | 5 | 1 | 1 | 66 | 100 | 44 | 39 | 73 | 64 |
Nucleus | 1 | 0 | 0 | 0 | 5 | 2 | 1 | 0 | 0 | 1 | 0 | 2 | 0 | 0 | 1 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 1 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 |
Nucleolus | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 3 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 2 |
SpindlePole | 0 | 1 | 1 | 0 | 2 | 0 | 3 | 8 | 1 | 5 | 1 | 2 | 1 | 2 | 2 | 12 | 3 | 5 | 0 | 3 | 0 | 1 | 6 | 3 |
Vac/Vac Membrane | 0 | 0 | 0 | 0 | 0 | 2 | 3 | 5 | 0 | 0 | 0 | 0 | 0 | 2 | 1 | 0 | 0 | 0 | 17 | 26 | 4 | 4 | 17 | 15 |
Unique Cell Count | 291 | 586 | 211 | 129 | 110 | 206 | 217 | 238 | 223 | 206 | 156 | 202 | 241 | 359 | 396 | 203 | 88 | 93 | 354 | 658 | 228 | 225 | 405 | 443 |
Labelled Cell Count | 303 | 621 | 237 | 140 | 126 | 232 | 245 | 275 | 282 | 252 | 191 | 253 | 254 | 390 | 437 | 240 | 111 | 113 | 354 | 658 | 228 | 225 | 405 | 443 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 4.8 | 4.2 | 5.6 | 5.4 | 6.1 | 5.0 | 5.1 | 5.3 | 4.5 | 5.2 | 4.8 | 5.2 | 5.6 | 5.7 | 5.4 | 5.3 | 7.0 | 6.8 | 6.1 | 6.7 | 6.5 |
Std Deviation (1e-4) | 0.7 | 0.7 | 1.2 | 1.0 | 5.5 | 0.8 | 1.0 | 0.9 | 0.9 | 1.0 | 0.9 | 1.0 | 1.1 | 1.4 | 1.5 | 1.2 | 2.0 | 1.1 | 1.6 | 1.9 | 1.8 |
Intensity Change (Log2) | – | – | – | -0.06 | 0.12 | -0.17 | -0.13 | -0.09 | -0.33 | -0.12 | -0.22 | -0.11 | 0.0 | 0.03 | -0.05 | -0.07 | 0.32 | 0.28 | 0.12 | 0.24 | 0.22 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Actin | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud Neck | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud Site | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cell Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cyto | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endoplasmic Reticulum | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endosome | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Golgi | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Mitochondria | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nuclear Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nuc | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleolus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Peroxisomes | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
SpindlePole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Vac | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cortical Patches | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cytoplasm | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Vacuole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 1.0938 | 1.4068 | 1.3627 | 1.1379 | 1.5314 | 1.0181 | 5.3321 | 4.691 | 4.7176 | 5.4087 | 4.745 | 4.4482 | 2.4368 | 2.2225 | 1.9832 | 1.6993 | 2.2457 | 1.8822 |
Actin | 0.0271 | 0.006 | 0.0145 | 0.0143 | 0.2084 | 0.0233 | 0.0317 | 0.0146 | 0.0557 | 0.0188 | 0.0483 | 0.0535 | 0.0875 | 0.0104 | 0.0324 | 0.0025 | 0.0182 | 0.0142 |
Bud | 0.0003 | 0.0003 | 0.0005 | 0.0001 | 0.002 | 0.0028 | 0.0036 | 0.0005 | 0.0043 | 0 | 0.0009 | 0.0003 | 0.0052 | 0.002 | 0.0026 | 0.0001 | 0.0005 | 0.0003 |
Bud Neck | 0.008 | 0.0014 | 0.0027 | 0.0022 | 0.0018 | 0.0229 | 0.0048 | 0.0013 | 0.0048 | 0.0011 | 0.0467 | 0.003 | 0.0196 | 0.0009 | 0.006 | 0.0047 | 0.0023 | 0.013 |
Bud Periphery | 0.0007 | 0.0009 | 0.0012 | 0.0005 | 0.0047 | 0.0022 | 0.0348 | 0.0015 | 0.0071 | 0.0002 | 0.0015 | 0.0007 | 0.0054 | 0.003 | 0.002 | 0.0009 | 0.0011 | 0.0013 |
Bud Site | 0.0342 | 0.0192 | 0.0054 | 0.0004 | 0.0029 | 0.0014 | 0.0075 | 0.0434 | 0.0258 | 0.0002 | 0.006 | 0.0009 | 0.0232 | 0.024 | 0.0189 | 0.0003 | 0.0017 | 0.0004 |
Cell Periphery | 0.023 | 0.0095 | 0.0078 | 0.0092 | 0.0015 | 0.0033 | 0.0105 | 0.014 | 0.0172 | 0.0019 | 0.0014 | 0.0027 | 0.0086 | 0.0105 | 0.0075 | 0.0049 | 0.003 | 0.0027 |
Cytoplasm | 0.3192 | 0.344 | 0.3857 | 0.5919 | 0.2303 | 0.5608 | 0.1889 | 0.4342 | 0.3787 | 0.3176 | 0.2186 | 0.3375 | 0.2302 | 0.3921 | 0.3172 | 0.4006 | 0.3468 | 0.3751 |
Cytoplasmic Foci | 0.1193 | 0.0131 | 0.0128 | 0.0312 | 0.064 | 0.0319 | 0.0163 | 0.0084 | 0.0248 | 0.0468 | 0.1019 | 0.0237 | 0.0435 | 0.0138 | 0.035 | 0.0037 | 0.0217 | 0.021 |
Eisosomes | 0.0031 | 0.0024 | 0.001 | 0.0017 | 0.0015 | 0.0002 | 0.0028 | 0.0018 | 0.0012 | 0.0005 | 0.0009 | 0.0005 | 0.0022 | 0.001 | 0.001 | 0.0008 | 0.0004 | 0.0003 |
Endoplasmic Reticulum | 0.019 | 0.0081 | 0.0162 | 0.0218 | 0.0075 | 0.0115 | 0.0458 | 0.0083 | 0.0146 | 0.002 | 0.0083 | 0.0254 | 0.0177 | 0.0229 | 0.012 | 0.0265 | 0.0057 | 0.0125 |
Endosome | 0.0118 | 0.0046 | 0.0091 | 0.0055 | 0.0304 | 0.0119 | 0.0335 | 0.0015 | 0.0067 | 0.0008 | 0.0341 | 0.0088 | 0.0174 | 0.0029 | 0.0087 | 0.0012 | 0.0178 | 0.0065 |
Golgi | 0.0083 | 0.0013 | 0.007 | 0.0026 | 0.0846 | 0.0139 | 0.0064 | 0.001 | 0.0117 | 0.0011 | 0.0284 | 0.0072 | 0.0156 | 0.0015 | 0.0086 | 0.0006 | 0.0191 | 0.0009 |
Lipid Particles | 0.0755 | 0.0096 | 0.0079 | 0.0099 | 0.0172 | 0.0043 | 0.0214 | 0.0033 | 0.0059 | 0.0015 | 0.1049 | 0.0055 | 0.0271 | 0.0042 | 0.0071 | 0.0009 | 0.0179 | 0.0037 |
Mitochondria | 0.003 | 0.0002 | 0.0246 | 0.0006 | 0.0323 | 0.0092 | 0.0133 | 0.0002 | 0.0209 | 0.0004 | 0.0216 | 0.0073 | 0.0102 | 0.0008 | 0.0026 | 0.0004 | 0.0201 | 0.0005 |
None | 0.253 | 0.5749 | 0.4805 | 0.3038 | 0.2981 | 0.2907 | 0.4762 | 0.4597 | 0.4086 | 0.5942 | 0.3368 | 0.5123 | 0.4401 | 0.502 | 0.5207 | 0.548 | 0.4441 | 0.5388 |
Nuclear Periphery | 0.0343 | 0.0005 | 0.0017 | 0.0005 | 0.0008 | 0.0008 | 0.0559 | 0.0006 | 0.0029 | 0.0001 | 0.0015 | 0.0007 | 0.0076 | 0.0008 | 0.0007 | 0.0004 | 0.0025 | 0.0003 |
Nucleolus | 0.0013 | 0.0003 | 0.0002 | 0.0001 | 0.0002 | 0.0003 | 0.0021 | 0.0006 | 0.0004 | 0.0001 | 0.0003 | 0.0001 | 0.0025 | 0.0005 | 0.0015 | 0.0001 | 0.0007 | 0.0001 |
Nucleus | 0.0046 | 0.0007 | 0.0008 | 0.0004 | 0.0003 | 0.0025 | 0.0199 | 0.001 | 0.0022 | 0.0003 | 0.0021 | 0.0003 | 0.0045 | 0.001 | 0.0014 | 0.0006 | 0.0015 | 0.0004 |
Peroxisomes | 0.0465 | 0.0002 | 0.0125 | 0.0009 | 0.0065 | 0.0008 | 0.0016 | 0.0003 | 0.0018 | 0.0084 | 0.0312 | 0.0072 | 0.0193 | 0.0022 | 0.0085 | 0.0001 | 0.0448 | 0.0067 |
Punctate Nuclear | 0.005 | 0.0006 | 0.0004 | 0.0006 | 0.0026 | 0.0027 | 0.0038 | 0.0012 | 0.0013 | 0.0008 | 0.003 | 0.001 | 0.0049 | 0.0015 | 0.002 | 0.0002 | 0.0285 | 0.0005 |
Vacuole | 0.0018 | 0.0023 | 0.0066 | 0.0017 | 0.0012 | 0.0022 | 0.0062 | 0.0026 | 0.0028 | 0.0031 | 0.0009 | 0.0013 | 0.0054 | 0.002 | 0.0033 | 0.0023 | 0.0012 | 0.0007 |
Vacuole Periphery | 0.0011 | 0.0001 | 0.0009 | 0.0001 | 0.001 | 0.0003 | 0.0131 | 0.0001 | 0.0005 | 0.0001 | 0.0007 | 0.0001 | 0.0023 | 0.0001 | 0.0004 | 0.0001 | 0.0006 | 0.0001 |
R1 | R2 | |||||||||
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G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 13.8679 | 9.7021 | 11.199 | 7.638 | 9.3811 | 2.4105 | 9.5373 | 6.9887 | 9.7922 | 11.6684 |
Translational Efficiency | 1.1442 | 1.0032 | 0.6632 | 1.0688 | 1.0178 | 6.8779 | 1.1751 | 1.5688 | 0.9322 | 0.8802 |
Dataset | Hit |
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Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
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37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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