Standard name
Human Ortholog
Description Mitochondrial protein; likely involved in translation of the mitochondrial OLI1 mRNA; exhibits genetic interaction with the OLI1 mRNA 5'-untranslated leader
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0.06 0.06 0 0 0 0 0.05 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0.05 0.14 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0.06 0 0 0 0 0 0 0
Cytoplasm 0 0 0.11 0.08 0.1 0.07 0.09 0.1 0 0 0.05 0.07 0.21 0.35 0.45 0.11 0.08 0.15 0 0 0 0 0 0
Endoplasmic Reticulum 0 0.05 0.06 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0.06 0 0 0 0 0 0
Endosome 0 0.11 0.08 0.16 0.21 0.07 0.05 0 0 0 0 0 0.26 0.24 0.26 0.17 0.2 0.19 0.15 0.12 0.12 0.08 0.09 0.07
Golgi 0 0.06 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0.06 0.06 0.05 0 0.08 0 0 0 0
Mitochondria 0.93 0.61 0.18 0.59 0.44 0.72 0.72 0.73 0.86 0.81 0.93 0.87 0.31 0.09 0.12 0.12 0.12 0.14 0.69 0.64 0.7 0.71 0.72 0.69
Nucleus 0 0 0 0 0 0 0.05 0.07 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0.07 0.06 0.06 0 0 0 0 0 0 0 0 0.07 0.09 0.09 0.11 0.13 0.11 0.05 0.05 0.06 0 0.05 0
SpindlePole 0 0.05 0.29 0.19 0.18 0.07 0.09 0.15 0.08 0.14 0.05 0.06 0.07 0.05 0.06 0.38 0.27 0.28 0 0 0 0 0 0
Vac/Vac Membrane 0.1 0.22 0.17 0 0.09 0.12 0.05 0.05 0.06 0.05 0 0.05 0.1 0.09 0.1 0.09 0.18 0.16 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 5 2 3 6 4 1 3 6 1 2 1 0 6 8 0 10 3 6 2 12 4 0 3 7
Bud 1 2 1 6 6 10 5 15 7 13 15 5 1 4 0 14 6 6 0 2 0 0 0 0
Bud Neck 0 0 2 2 1 2 4 2 0 1 1 2 0 0 0 0 3 1 0 1 0 1 1 4
Bud Site 2 1 0 1 0 0 0 5 1 1 0 3 0 0 0 4 1 3 – – – – – –
Cell Periphery 4 16 9 2 3 0 1 3 0 1 3 1 2 4 8 17 16 11 0 0 0 0 0 0
Cytoplasm 6 11 7 11 19 21 25 35 6 9 12 18 29 59 92 31 23 44 1 2 0 4 1 5
Endoplasmic Reticulum 6 19 4 5 5 8 8 3 2 6 3 7 3 7 0 15 11 19 0 2 0 1 1 2
Endosome 4 37 5 23 39 20 14 16 6 5 0 1 35 41 54 48 58 56 34 52 19 15 19 17
Golgi 0 22 2 3 4 0 1 0 0 0 0 0 5 9 4 17 18 14 5 34 3 6 6 10
Mitochondria 205 213 12 84 81 212 200 262 211 174 245 228 42 16 25 35 33 43 162 287 115 139 156 174
Nucleus 2 1 2 1 7 10 15 25 4 8 5 5 2 3 1 13 16 8 0 0 0 0 0 0
Nuclear Periphery 2 3 0 0 0 2 7 1 3 2 3 5 0 0 0 0 1 0 2 4 0 0 2 1
Nucleolus 2 0 0 0 0 0 1 1 1 2 2 2 0 0 0 0 2 0 1 0 0 0 0 0
Peroxisomes 2 24 4 9 5 8 5 9 4 1 0 0 9 16 19 33 36 32 10 22 9 8 11 10
SpindlePole 9 16 19 27 34 21 24 53 19 31 13 16 9 9 12 110 77 83 2 3 2 4 1 1
Vac/Vac Membrane 21 76 11 3 16 35 13 18 15 10 7 13 13 16 20 27 52 49 5 12 6 8 8 10
Unique Cell Count 220 347 66 143 186 296 278 359 244 216 263 261 136 171 204 289 285 297 236 447 165 196 218 252
Labelled Cell Count 271 443 81 183 224 350 326 454 280 266 310 306 156 192 235 374 356 375 236 447 165 196 218 252


Mitochondria

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.5 5.8 5.9 5.5 5.7 4.8 4.8 5.2 4.3 4.4 4.0 4.0 6.3 6.1 5.9 6.4 6.8 6.2 5.7 5.8 6.2
Std Deviation (1e-4) 0.6 1.0 0.9 1.1 1.1 1.3 1.0 1.4 0.9 0.7 1.2 0.9 1.2 1.2 1.2 1.1 1.4 1.4 1.1 1.1 1.5
Intensity Change (Log2) – – – -0.09 -0.04 -0.3 -0.29 -0.17 -0.45 -0.4 -0.55 -0.53 0.09 0.06 0.02 0.12 0.22 0.08 -0.03 -0.03 0.09


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud 0 0 0 0 0 0 – – 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 – – 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 – – 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 – – 0 0 0 0 0 0 0
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum 0 0 0 0 0 0 – – 0 0 0 0 0 0 0
Endosome 1.7 2.5 0 0 0 0 – – 0 3.0 2.9 3.2 1.9 2.4 2.2
Golgi 0 0 0 0 0 0 – – 0 0 0 0 0 0 0
Mitochondria 5.5 3.7 0 0 8.5 0 – – 0 1.9 -1.9 -1.2 -1.3 -1.4 -0.8
Nuclear Periphery 0 0 0 0 0 0 – – 0 0 0 0 0 0 0
Nuc – – – – – – – – – – – – – – –
Nucleolus 0 0 0 0 0 0 – – 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 – – 0 0 0.8 0.8 1.3 1.5 1.2
SpindlePole -1.6 -1.8 0 0 -2.8 0 – – 0 -4.3 -5.0 -5.1 1.4 -0.3 -0.1
Vac – – – – – – – – – – – – – – –
Cortical Patches 0 0 0 0 0 0 – – 0 0 0 0 0 0 0
Cytoplasm -0.7 -0.1 -1.0 -0.4 -0.2 0 – – -1.0 1.9 3.7 5.1 0 -0.7 0.9
Nucleus 0 0 0 0 0 0 – – 0 0 0 0 0 0 0
Vacuole 0 -1.8 -1.1 0 0 -2.7 – – 0 -1.5 -1.6 -1.5 -1.7 0.3 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 2.058 1.6484 1.3672 1.2556 2.1411 1.4068 1.7245 1.3622 1.5229 1.5941 2.0427 1.2587 2.5564 2.1263 2.0458 2.1496 2.8034 2.2672
Actin 0.0392 0.0153 0.0048 0.0106 0.0082 0.0029 0.0505 0.0154 0.0237 0.0069 0.0215 0.0052 0.0872 0.0246 0.0513 0.0453 0.037 0.0599
Bud 0.0013 0.0022 0.0006 0.001 0.0025 0.0003 0.0018 0.0022 0.0018 0.0011 0.0027 0.0009 0.0007 0.0023 0.0071 0.0027 0.0101 0.0007
Bud Neck 0.0088 0.0021 0.0008 0.0003 0.002 0.0004 0.0015 0.0011 0.0032 0.0008 0.0017 0.0023 0.0018 0.0009 0.0008 0.0005 0.0014 0.0014
Bud Periphery 0.0026 0.0026 0.001 0.0022 0.0028 0.0027 0.0041 0.0035 0.0037 0.0017 0.009 0.0012 0.0012 0.0037 0.0125 0.0065 0.0137 0.0024
Bud Site 0.0104 0.0197 0.0032 0.0011 0.0059 0.0005 0.0055 0.0282 0.0252 0.0145 0.0045 0.0007 0.012 0.0351 0.0162 0.0034 0.0267 0.0013
Cell Periphery 0.0023 0.001 0.0003 0.0002 0.0003 0.0004 0.0011 0.002 0.0011 0.0006 0.002 0.0003 0.0004 0.0015 0.0009 0.0003 0.0005 0.0004
Cytoplasm 0.0153 0.0111 0.0053 0.001 0.005 0.0005 0.0131 0.0078 0.0074 0.0008 0.0235 0.0043 0.0228 0.0067 0.0063 0.0016 0.002 0.0047
Cytoplasmic Foci 0.0467 0.042 0.0438 0.0187 0.0607 0.0111 0.0853 0.046 0.0516 0.023 0.0304 0.0265 0.0701 0.0436 0.0462 0.0318 0.0631 0.0344
Eisosomes 0.0025 0.001 0.0004 0.0001 0.0001 0.0002 0.0011 0.0006 0.0005 0.0002 0.0011 0.0003 0.0007 0.0008 0.0012 0.0008 0.0005 0.0009
Endoplasmic Reticulum 0.0064 0.0014 0.0009 0.0002 0.0021 0.0005 0.0051 0.0025 0.0011 0.0009 0.0037 0.0011 0.0133 0.0053 0.0014 0.0006 0.0007 0.0007
Endosome 0.1282 0.1267 0.1016 0.0379 0.1972 0.0247 0.1125 0.0886 0.0624 0.0404 0.1309 0.0775 0.0643 0.0659 0.0539 0.0384 0.0418 0.0629
Golgi 0.079 0.0907 0.0599 0.0355 0.1052 0.0338 0.0592 0.0616 0.0577 0.0259 0.0605 0.0616 0.0615 0.1029 0.0651 0.0641 0.0489 0.1134
Lipid Particles 0.0603 0.0197 0.0418 0.0099 0.0121 0.0257 0.0897 0.0241 0.0318 0.0293 0.0176 0.0295 0.0729 0.0114 0.009 0.0188 0.0699 0.0183
Mitochondria 0.462 0.5996 0.6601 0.8149 0.5512 0.8158 0.4261 0.6457 0.6335 0.8179 0.5566 0.6991 0.4549 0.6177 0.6685 0.7404 0.6308 0.6412
None 0.014 0.0121 0.0083 0.0004 0.0005 0.0004 0.0422 0.0108 0.0111 0.0007 0.0341 0.0184 0.0502 0.0098 0.0288 0.0014 0.0007 0.0005
Nuclear Periphery 0.0129 0.0004 0.001 0.0001 0.0016 0.0002 0.003 0.0011 0.0008 0.0008 0.0252 0.0013 0.0059 0.0007 0.0007 0.0017 0.001 0.0002
Nucleolus 0.0017 0.0006 0.0012 0.0001 0.0003 0.0001 0.0033 0.0014 0.0012 0.0009 0.0022 0.0005 0.0008 0.0004 0.0008 0.0014 0.0018 0.0002
Nucleus 0.013 0.0007 0.0016 0.0001 0.0008 0.0001 0.0074 0.0007 0.001 0.0005 0.0155 0.0008 0.0013 0.0003 0.0009 0.0011 0.001 0.0002
Peroxisomes 0.0759 0.0387 0.0515 0.0614 0.0227 0.0762 0.0581 0.0458 0.0714 0.0243 0.0209 0.059 0.0599 0.0614 0.022 0.0322 0.0373 0.0518
Punctate Nuclear 0.0043 0.0005 0.0022 0.0002 0.0009 0.0001 0.007 0.0007 0.0021 0.0007 0.0025 0.0005 0.0131 0.0006 0.0009 0.0035 0.0043 0.0003
Vacuole 0.0078 0.0073 0.0059 0.0018 0.0103 0.0013 0.0149 0.0062 0.0046 0.0053 0.0238 0.0041 0.0026 0.0026 0.0037 0.0017 0.0046 0.0019
Vacuole Periphery 0.0052 0.0046 0.0037 0.0023 0.0077 0.0019 0.0073 0.004 0.0031 0.0027 0.0097 0.0047 0.0024 0.0018 0.0017 0.0019 0.0022 0.0021

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 9.8123 7.3568 3.7793 13.3654 9.4389 8.2251 9.5842 12.1649 13.3048 14.2702
Translational Efficiency 0.6216 0.6493 1.5509 0.5717 0.5718 0.7143 0.6224 0.4848 0.4834 0.4848

Hit Data

Dataset Hit
Protein Concentration ✔
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Mitochondrial protein; likely involved in translation of the mitochondrial OLI1 mRNA; exhibits genetic interaction with the OLI1 mRNA 5'-untranslated leader
Localization
Cell Percentages mitochondrion (80%)
Cell Cycle Regulation No
Subcompartmental Group mito-1

Aep2

Aep2


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Aep2-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available