ORF
Human Ortholog
Description Protein of unknown function that associates with ribosomes; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery and bud; not an essential gene; protein abundance increases in response to DNA replication stress; YMR295C has a paralog, YGR273C, that arose from the whole genome duplication
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0.23 0.25 0.27 0.46 0.24 0.28 0.26 – 0.28 0.24 0.28 0.22 0.34 0.28 0.25 0.14 0.08 0.11 0.12 0.11 0.13 0.12 0.15 0.19
Bud 0.08 0.17 0.16 0.21 0.19 0.18 0.15 – 0.17 0.12 0.15 0.14 0.13 0.11 0.14 0.06 0 0.06 0.31 0.44 0.33 0.35 0.32 0.31
Bud Neck 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0.09 0.12 0.08 0.17 0.11 0.08
Bud Site 0 0.08 0.07 0.08 0 0 0 – 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0.28 0.25 0.3 0.36 0.14 0.2 0.34 – 0.29 0.24 0.33 0.29 0.45 0.52 0.62 0.17 0.17 0.17 0.21 0.14 0.21 0.1 0.18 0.12
Cytoplasm 0.09 0.08 0.09 0.07 0.17 0.2 0.23 – 0.27 0.26 0.31 0.34 0.07 0.08 0.07 0.14 0.14 0.11 0 0 0 0 0 0
Endoplasmic Reticulum 0.14 0.07 0 0.07 0.1 0.11 0.1 – 0.06 0.06 0.1 0.14 0.13 0.1 0.1 0 0.05 0 0.06 0 0 0 0.05 0.11
Endosome 0.09 0.08 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0.09 0 0 – 0 0 0 0 0.06 0 0 0.13 0.18 0.21 0 0 0 0 0 0
Mitochondria 0 0.05 0 0 0 0.06 0 – 0 0 0 0 0 0 0 0.18 0 0.06 0.05 0 0.08 0.15 0.11 0.09
Nucleus 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.11 0.16 0.1 0.07 0.07 0.13 0.07 – 0.1 0.11 0.07 0 0 0 0 0.11 0.14 0.13 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0.05 0.05 0.08 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.17 0.1 0.14 0.06 0.22 0.16 0.17 – 0.16 0.24 0.15 0.13 0.05 0 0 0.28 0.36 0.24 0.06 0.05 0.08 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 39 28 50 108 55 61 107 – 143 112 103 93 62 79 56 20 9 16 29 18 33 14 39 52
Bud 13 19 29 49 44 39 60 – 87 56 53 60 24 30 31 8 4 9 73 72 86 44 88 85
Bud Neck 2 0 2 1 0 1 0 – 0 0 1 1 0 0 0 1 0 1 21 20 22 21 29 23
Bud Site 5 9 12 19 0 8 2 – 5 4 4 4 5 3 2 2 1 1 – – – – – –
Cell Periphery 47 28 55 86 32 42 138 – 153 113 121 123 81 148 140 25 20 25 50 22 54 12 50 32
Cytoplasm 15 9 17 16 38 43 93 – 141 122 111 142 12 23 16 20 17 16 2 3 0 0 3 4
Endoplasmic Reticulum 23 8 7 16 23 24 43 – 32 26 36 59 23 29 23 4 6 5 14 2 6 2 13 32
Endosome 15 9 7 7 1 1 10 – 3 3 0 4 6 11 1 4 4 6 2 1 1 0 2 3
Golgi 3 0 3 7 21 1 10 – 9 17 7 4 11 11 5 19 21 31 3 0 1 1 0 3
Mitochondria 6 6 1 10 0 12 1 – 6 6 4 12 6 7 5 26 2 9 11 4 22 19 29 25
Nucleus 1 2 1 2 0 5 2 – 6 4 1 1 2 1 1 0 0 1 0 1 1 0 0 0
Nuclear Periphery 0 0 0 0 0 1 0 – 2 2 2 3 0 2 2 0 0 0 4 3 3 0 3 5
Nucleolus 19 18 19 16 17 27 28 – 54 49 24 18 2 3 0 16 16 19 1 0 2 0 1 0
Peroxisomes 1 0 0 2 0 1 0 – 0 0 0 0 6 11 8 1 0 0 0 0 0 0 0 0
SpindlePole 8 6 15 6 10 1 8 – 1 4 3 2 2 6 2 2 1 3 0 1 4 0 0 0
Vac/Vac Membrane 29 11 25 15 51 35 70 – 84 110 55 54 9 9 3 40 43 36 15 7 20 3 6 6
Unique Cell Count 168 114 183 236 227 215 410 519 462 362 420 181 283 226 145 118 149 236 165 266 125 275 281
Labelled Cell Count 226 153 243 360 292 302 572 726 628 525 580 251 373 295 188 144 178 236 165 266 125 275 281


Bud, Cell Periphery

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 24.4 22.0 22.1 20.0 23.6 16.7 22.2 – 20.0 20.6 19.6 19.1 24.8 24.5 25.3 38.9 36.7 40.1 23.5 22.9 22.7
Std Deviation (1e-4) 15.9 13.5 11.0 7.0 10.4 6.7 7.7 – 6.4 6.1 6.7 6.2 12.9 12.5 11.5 24.5 20.8 25.1 11.8 8.9 8.3
Intensity Change (Log2) – – – -0.14 0.09 -0.41 0.01 – -0.14 -0.1 -0.17 -0.21 0.17 0.15 0.2 0.82 0.73 0.86 0.09 0.06 0.04


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 3.9 -0.7 0.2 -0.3 – 0.1 -0.8 0.3 -1.4 1.4 0.1 -0.6 -3.0 -4.2 -3.8
Bud 1.3 0.9 0.6 -0.4 – 0.3 -1.3 -0.4 -0.5 -0.7 -1.7 -0.6 -2.9 -3.4 -2.8
Bud Neck 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud Site 0.6 -3.9 -1.3 0 – 0 0 -3.6 0 -1.7 -3.3 -3.1 -2.3 -2.4 -2.8
Cell Periphery 1.4 -3.9 -2.4 0.9 – -0.1 -1.5 0.8 -0.2 2.9 4.7 6.4 -2.7 -2.6 -2.8
Cytoplasm -0.9 2.2 3.0 3.9 – 5.0 4.8 5.6 6.3 -0.9 -0.4 -0.8 1.3 1.4 0.4
Endoplasmic Reticulum 1.3 2.4 2.7 2.7 – 1.2 0.9 2.5 3.7 3.1 2.5 2.4 0 0.5 -0.2
Endosome -0.5 0 0 -0.9 – 0 0 0 0 -0.3 0 0 0 0 0.1
Golgi 0 3.3 0 0 – 0 1.3 0 0 2.2 1.4 0 4.1 5.1 5.7
Mitochondria 0 0 2.8 0 – 0 0 0 0 0 0 0 5.7 0 0
Nucleus 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Nucleolus -1.3 -1.0 0.7 -1.5 – 0 0.1 -1.5 -2.9 -3.8 -4.6 -5.0 0.2 0.8 0.7
Peroxisomes 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
SpindlePole -2.6 -1.6 -3.9 -3.6 – 0 -5.0 -4.5 -5.3 -3.2 -3.1 -3.7 -2.8 -2.8 -2.5
Vacuole -2.5 2.3 0.7 1.0 – 0.8 2.9 0.5 -0.3 -2.8 -4.2 -4.9 3.1 4.6 2.5

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 19.1172 14.7259 14.7893 14.2287 14.1064 16.5151 24.0263 14.7793 18.22 18.2023 17.0227 20.6477 24.9129 18.4723 20.3412 24.499 22.3408 22.9569
Actin 0.0587 0.0667 0.0506 0.0809 0.116 0.0473 0.0514 0.0525 0.0291 0.1246 0.009 0.0868 0.0515 0.0356 0.0468 0.0157 0.0145 0.0872
Bud 0.0244 0.0354 0.2556 0.1139 0.0762 0.0162 0.0087 0.0702 0.2527 0.1777 0.0719 0.0139 0.0167 0.0712 0.2824 0.118 0.0977 0.0154
Bud Neck 0.0342 0.0114 0.0116 0.0162 0.1613 0.4996 0.0288 0.0092 0.0012 0.0015 0.0033 0.5689 0.0125 0.0098 0.0088 0.0048 0.1383 0.4579
Bud Periphery 0.0658 0.0625 0.3986 0.5118 0.2882 0.1239 0.0448 0.1131 0.4886 0.4211 0.5782 0.1011 0.0819 0.1259 0.4255 0.4723 0.3676 0.1191
Bud Site 0.197 0.4641 0.0892 0.0222 0.0076 0.0103 0.1037 0.3599 0.0465 0.0206 0.0048 0.0113 0.1311 0.4203 0.0842 0.0179 0.1559 0.0084
Cell Periphery 0.4374 0.2616 0.0599 0.148 0.0923 0.179 0.5338 0.1402 0.0601 0.0611 0.2845 0.0993 0.475 0.1964 0.0755 0.1497 0.0549 0.1804
Cytoplasm 0.0215 0.0181 0.0086 0.005 0.0054 0.0062 0.0482 0.0232 0.0027 0.0062 0.0013 0.0095 0.0201 0.0319 0.0036 0.0031 0.0018 0.0067
Cytoplasmic Foci 0.0163 0.0067 0.0094 0.0012 0.0129 0.0064 0.0173 0.0361 0.0021 0.0057 0 0.0063 0.0225 0.0081 0.0027 0.0004 0.0019 0.0086
Eisosomes 0.0149 0.0034 0.0004 0.0008 0.0037 0.0016 0.0068 0.0017 0.0002 0.0004 0.0006 0.0042 0.0089 0.0007 0.0012 0.0006 0.0012 0.0038
Endoplasmic Reticulum 0.0134 0.0028 0.0046 0.0012 0.0081 0.0038 0.0217 0.0041 0.0024 0.0038 0.0013 0.0077 0.0241 0.0028 0.0028 0.0016 0.0022 0.0033
Endosome 0.0263 0.008 0.0203 0.0027 0.0756 0.0152 0.0096 0.025 0.0152 0.0182 0.0002 0.0055 0.0301 0.0183 0.0023 0.0009 0.0688 0.0163
Golgi 0.0064 0.0033 0.0042 0.0062 0.0353 0.03 0.0069 0.0133 0.0108 0.0233 0.0001 0.0074 0.012 0.0046 0.0054 0.0002 0.0082 0.0181
Lipid Particles 0.0109 0.0053 0.0082 0.0023 0.034 0.0127 0.0134 0.0133 0.0013 0.0074 0.0003 0.0133 0.0251 0.0067 0.0031 0.0027 0.0023 0.0112
Mitochondria 0.0101 0.003 0.0154 0.0176 0.0395 0.0028 0.0035 0.0039 0.0184 0.061 0.0022 0.0062 0.005 0.0024 0.0058 0.0682 0.015 0.003
None 0.0054 0.0005 0.0013 0.0002 0.0005 0.0001 0.0053 0.0008 0.0002 0.0008 0.0002 0.0177 0.0023 0.0004 0.0006 0.0002 0.0003 0.0001
Nuclear Periphery 0.005 0.0015 0.0052 0.0009 0.0032 0.0009 0.0079 0.0016 0.013 0.0031 0.0007 0.0007 0.0081 0.0014 0.0018 0.001 0.0039 0.0008
Nucleolus 0.0034 0.0025 0.0034 0.0197 0.0015 0.0018 0.0142 0.0068 0.0011 0.001 0.001 0.0031 0.0023 0.0132 0.0072 0.0017 0.0014 0.0014
Nucleus 0.0026 0.0018 0.0064 0.0064 0.0023 0.0023 0.0041 0.0057 0.0023 0.0023 0.0015 0.0019 0.0021 0.0031 0.0022 0.0028 0.0026 0.0019
Peroxisomes 0.0035 0.0008 0.0041 0.0018 0.0024 0.0019 0.0044 0.0011 0.0009 0.0071 0.0001 0.0054 0.0092 0.0009 0.0015 0.0025 0.0004 0.0065
Punctate Nuclear 0.0016 0.0002 0.0052 0.0002 0.0006 0.0002 0.0041 0.001 0.0001 0.0004 0 0.0005 0.0022 0.0003 0.0002 0 0.0002 0.0003
Vacuole 0.0385 0.0381 0.0329 0.0369 0.0242 0.034 0.0588 0.109 0.0412 0.0452 0.0338 0.0258 0.051 0.0404 0.0324 0.1192 0.0449 0.0442
Vacuole Periphery 0.0025 0.0022 0.0046 0.004 0.0093 0.0037 0.0026 0.0083 0.01 0.0074 0.0049 0.0034 0.0062 0.0056 0.0042 0.0165 0.0161 0.0055

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 178.328 192.6256 103.5058 71.5947 186.6539 135.5285 221.2078 136.5517 134.868 131.1511
Translational Efficiency 2.1524 2.3572 1.7682 2.6679 1.8665 3.0799 1.7815 2.3402 1.6527 1.9972

Hit Data

Dataset Hit
Protein Concentration ✔
Protein Localization ✔
Gene Expression ✘
Translational Efficiency ✘
ORF
Human Ortholog
Description Protein of unknown function that associates with ribosomes; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery and bud; not an essential gene; protein abundance increases in response to DNA replication stress; YMR295C has a paralog, YGR273C, that arose from the whole genome duplication
Localization
Cell Percentages cell periphery (48%)
Cell Cycle Regulation No
Subcompartmental Group cell periphery-3

YMR295C

YMR295C


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Ymr295c-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available