Standard name | |
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Human Ortholog | |
Description | Cell wall protein with similarity to glucanases; may play a role in conjugation during mating based on mutant phenotype and its regulation by Ste12p; SWC10 has a paralog, SCW4, that arose from the whole genome duplication |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 1 | 4 | 2 | 0 | 2 | 0 |
Bud | 1 | 1 | 0 | 2 | 0 | 4 | 9 | 9 | 8 | 9 | 13 | 9 | 0 | 0 | 0 | 3 | 1 | 0 | 1 | 13 | 10 | 3 | 4 | 3 |
Bud Neck | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 2 | 0 | 0 | 1 | 1 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 4 | 0 | 1 | 0 | 0 | 0 | 0 |
Cytoplasm | 82 | 379 | 320 | 324 | 301 | 407 | 450 | 396 | 469 | 471 | 422 | 532 | 87 | 164 | 158 | 135 | 158 | 151 | 20 | 120 | 34 | 13 | 13 | 16 |
Endoplasmic Reticulum | 2 | 3 | 1 | 1 | 0 | 1 | 0 | 0 | 2 | 3 | 3 | 0 | 3 | 2 | 4 | 9 | 6 | 5 | 0 | 0 | 1 | 4 | 5 | 4 |
Endosome | 8 | 9 | 14 | 13 | 26 | 11 | 7 | 8 | 8 | 6 | 3 | 6 | 0 | 0 | 0 | 0 | 0 | 2 | 13 | 31 | 13 | 4 | 17 | 15 |
Golgi | 0 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 2 | 1 | 1 | 1 | 1 | 3 | 0 | 2 | 3 |
Mitochondria | 11 | 37 | 22 | 5 | 9 | 36 | 83 | 69 | 64 | 93 | 81 | 62 | 2 | 0 | 0 | 7 | 0 | 1 | 4 | 10 | 12 | 6 | 11 | 11 |
Nucleus | 8 | 10 | 16 | 30 | 32 | 65 | 134 | 116 | 69 | 65 | 40 | 31 | 1 | 2 | 0 | 7 | 8 | 8 | 3 | 4 | 2 | 0 | 0 | 1 |
Nuclear Periphery | 0 | 1 | 4 | 9 | 4 | 5 | 2 | 13 | 0 | 9 | 9 | 5 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 2 | 0 | 2 | 5 |
Nucleolus | 1 | 1 | 2 | 0 | 0 | 2 | 1 | 1 | 3 | 3 | 0 | 2 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 1 | 1 | 1 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 |
SpindlePole | 0 | 1 | 0 | 2 | 0 | 0 | 0 | 4 | 1 | 0 | 0 | 3 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 9 | 2 | 0 | 3 | 3 |
Vac/Vac Membrane | 71 | 161 | 224 | 210 | 321 | 434 | 564 | 550 | 458 | 459 | 283 | 353 | 11 | 9 | 4 | 54 | 58 | 55 | 119 | 370 | 406 | 153 | 290 | 282 |
Unique Cell Count | 159 | 537 | 491 | 472 | 550 | 782 | 995 | 950 | 847 | 899 | 672 | 815 | 100 | 172 | 165 | 185 | 207 | 194 | 171 | 580 | 497 | 192 | 362 | 355 |
Labelled Cell Count | 184 | 605 | 606 | 598 | 694 | 966 | 1251 | 1166 | 1083 | 1118 | 854 | 1003 | 105 | 177 | 167 | 220 | 233 | 229 | 171 | 580 | 497 | 192 | 362 | 355 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 4.8 | 4.1 | 4.6 | 4.8 | 6.2 | 5.6 | 5.8 | 6.1 | 5.7 | 5.6 | 5.3 | 5.3 | 4.8 | 5.0 | 4.9 | 5.2 | 5.4 | 5.3 | 5.6 | 5.9 | 6.2 |
Std Deviation (1e-4) | 1.3 | 0.7 | 1.4 | 1.7 | 2.1 | 2.0 | 2.1 | 2.1 | 2.2 | 2.0 | 1.9 | 1.9 | 1.1 | 1.0 | 1.1 | 1.2 | 1.3 | 1.1 | 1.3 | 1.3 | 1.3 |
Intensity Change (Log2) | – | – | – | 0.06 | 0.44 | 0.28 | 0.34 | 0.4 | 0.32 | 0.29 | 0.2 | 0.21 | 0.07 | 0.12 | 0.11 | 0.18 | 0.23 | 0.2 | 0.3 | 0.35 | 0.44 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Actin | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Bud Neck | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Bud Site | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Cell Periphery | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Cyto | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endoplasmic Reticulum | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Endosome | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Golgi | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Mitochondria | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Nuclear Periphery | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Nuc | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleolus | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Peroxisomes | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
SpindlePole | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Vac | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cortical Patches | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Cytoplasm | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Nucleus | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Vacuole | – | – | – | – | – | – | – | – | – | – | – | – | -3.9 | -4.3 | -4.1 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | -1.4935 | -0.0961 | -0.576 | -1.1522 | -0.92 | -0.4668 | 0.2333 | 1.4436 | 1.01 | 0.6593 | 0.4258 | 0.7432 | -1.1946 | 0.1787 | -0.3376 | -0.1862 | -0.4076 | 0.089 |
Actin | 0.0016 | 0.0009 | 0.0003 | 0.0002 | 0 | 0.0031 | 0.0126 | 0.0001 | 0.0068 | 0.0002 | 0.001 | 0.0001 | 0.0249 | 0.0001 | 0.0001 | 0.0322 | 0.0294 | 0.0013 |
Bud | 0.0006 | 0.003 | 0.0005 | 0.0006 | 0.0003 | 0.0004 | 0.0023 | 0.0011 | 0.002 | 0.0013 | 0.0017 | 0.0012 | 0.0006 | 0.0004 | 0.0004 | 0.0004 | 0.0061 | 0.0004 |
Bud Neck | 0.0011 | 0.0016 | 0.0018 | 0.001 | 0.0017 | 0.0062 | 0.001 | 0.0042 | 0.0051 | 0.0022 | 0.0022 | 0.0061 | 0.0004 | 0.0093 | 0.0007 | 0.0007 | 0.0012 | 0.0027 |
Bud Periphery | 0.0013 | 0.0031 | 0.001 | 0.0021 | 0.0006 | 0.001 | 0.0033 | 0.0018 | 0.003 | 0.0018 | 0.0029 | 0.0016 | 0.0015 | 0.0005 | 0.0009 | 0.0005 | 0.0052 | 0.0006 |
Bud Site | 0.0018 | 0.0045 | 0.0021 | 0.0016 | 0.0023 | 0.001 | 0.0048 | 0.0134 | 0.0146 | 0.0028 | 0.014 | 0.0021 | 0.0012 | 0.0063 | 0.0021 | 0.0008 | 0.0464 | 0.0015 |
Cell Periphery | 0.0009 | 0.0016 | 0.0015 | 0.0007 | 0.0006 | 0.001 | 0.0008 | 0.0016 | 0.0016 | 0.0007 | 0.0007 | 0.0016 | 0.0006 | 0.0011 | 0.0011 | 0.0005 | 0.0018 | 0.0015 |
Cytoplasm | 0.0871 | 0.0693 | 0.0512 | 0.0288 | 0.0315 | 0.1003 | 0.0998 | 0.1117 | 0.0599 | 0.053 | 0.0211 | 0.0908 | 0.1207 | 0.1582 | 0.0943 | 0.0591 | 0.0309 | 0.0914 |
Cytoplasmic Foci | 0.0552 | 0.0309 | 0.0465 | 0.0481 | 0.0839 | 0.058 | 0.0785 | 0.0562 | 0.0585 | 0.0675 | 0.0442 | 0.063 | 0.0603 | 0.0476 | 0.0342 | 0.043 | 0.0288 | 0.0538 |
Eisosomes | 0.0005 | 0.0002 | 0.0002 | 0.0001 | 0 | 0.0001 | 0.0004 | 0.0001 | 0.0002 | 0.0001 | 0.0001 | 0.0001 | 0.0008 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0 |
Endoplasmic Reticulum | 0.0064 | 0.0059 | 0.0039 | 0.0049 | 0.002 | 0.0042 | 0.0082 | 0.0042 | 0.0038 | 0.002 | 0.0031 | 0.0035 | 0.008 | 0.0122 | 0.0067 | 0.0072 | 0.0015 | 0.0046 |
Endosome | 0.165 | 0.1501 | 0.2293 | 0.2961 | 0.2665 | 0.1833 | 0.2452 | 0.1989 | 0.3073 | 0.3445 | 0.376 | 0.2641 | 0.2243 | 0.2171 | 0.2701 | 0.418 | 0.2475 | 0.2074 |
Golgi | 0.0026 | 0.0043 | 0.0043 | 0.0051 | 0.0027 | 0.0027 | 0.0098 | 0.0037 | 0.0132 | 0.0048 | 0.0104 | 0.005 | 0.0144 | 0.0033 | 0.0042 | 0.0057 | 0.0102 | 0.0212 |
Lipid Particles | 0.0133 | 0.012 | 0.0283 | 0.0082 | 0.0116 | 0.0138 | 0.0172 | 0.0244 | 0.0211 | 0.0194 | 0.0267 | 0.02 | 0.0339 | 0.0169 | 0.021 | 0.0192 | 0.0066 | 0.0119 |
Mitochondria | 0.0067 | 0.0041 | 0.0133 | 0.0141 | 0.0053 | 0.0027 | 0.0169 | 0.005 | 0.0422 | 0.0066 | 0.0053 | 0.0077 | 0.0422 | 0.0019 | 0.0266 | 0.0025 | 0.0053 | 0.0056 |
None | 0.4239 | 0.3055 | 0.1953 | 0.2272 | 0.1316 | 0.1398 | 0.2612 | 0.1197 | 0.1526 | 0.0909 | 0.0966 | 0.1267 | 0.2971 | 0.171 | 0.1543 | 0.0244 | 0.0035 | 0.0567 |
Nuclear Periphery | 0.0078 | 0.0094 | 0.0114 | 0.0066 | 0.0037 | 0.0089 | 0.0102 | 0.0044 | 0.0052 | 0.0033 | 0.0053 | 0.0044 | 0.007 | 0.0083 | 0.0122 | 0.0052 | 0.0013 | 0.0064 |
Nucleolus | 0.0029 | 0.006 | 0.0069 | 0.0066 | 0.0211 | 0.0066 | 0.0041 | 0.0073 | 0.007 | 0.0061 | 0.0086 | 0.0089 | 0.0015 | 0.0015 | 0.003 | 0.0104 | 0.0026 | 0.0039 |
Nucleus | 0.007 | 0.0105 | 0.0111 | 0.0149 | 0.0241 | 0.0296 | 0.0079 | 0.0125 | 0.015 | 0.0075 | 0.0086 | 0.0181 | 0.0032 | 0.0042 | 0.0076 | 0.0078 | 0.0018 | 0.0262 |
Peroxisomes | 0.0014 | 0.0016 | 0.0036 | 0.0027 | 0.0025 | 0.0022 | 0.0066 | 0.0012 | 0.0057 | 0.0138 | 0.0116 | 0.0025 | 0.0038 | 0.0008 | 0.0011 | 0.0011 | 0.0014 | 0.0038 |
Punctate Nuclear | 0.0034 | 0.0021 | 0.0048 | 0.0016 | 0.0046 | 0.0033 | 0.0079 | 0.0017 | 0.003 | 0.0108 | 0.0029 | 0.0035 | 0.003 | 0.0014 | 0.0014 | 0.0019 | 0.0002 | 0.0007 |
Vacuole | 0.1802 | 0.3419 | 0.3452 | 0.2842 | 0.3617 | 0.3953 | 0.1766 | 0.3773 | 0.2393 | 0.3317 | 0.3257 | 0.3352 | 0.128 | 0.304 | 0.3023 | 0.3194 | 0.5244 | 0.4618 |
Vacuole Periphery | 0.0291 | 0.0315 | 0.0375 | 0.0446 | 0.0417 | 0.0366 | 0.0245 | 0.0494 | 0.0329 | 0.029 | 0.0314 | 0.0339 | 0.0225 | 0.0337 | 0.0555 | 0.04 | 0.0438 | 0.0367 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 706.839 | 309.4018 | 80.0748 | 107.6886 | 512.9417 | 401.53 | 226.5289 | 129.7351 | 94.2984 | 447.8906 |
Translational Efficiency | 1.3777 | 1.7588 | 1.5407 | 1.2957 | 1.5029 | 2.1293 | 1.5974 | 1.4343 | 1.478 | 1.361 |
Dataset | Hit |
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Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✔ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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