Standard name
Human Ortholog
Description Karyopherin/importin that interacts with the nuclear pore complex; acts as the nuclear import receptor for specific proteins, including Pdr1p, Yap1p, Ste12p, and Aft1p
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.85 0.96 0.72 0.4 0.48 0.52 0.51 0.44 0.46 0.45 0.39 0.32 0.75 0.76 0.66 0.86 0.78 0.84 0.69 0.69 0.66 0.64 0.72 0.67
Endoplasmic Reticulum 0 0 0.06 0.11 0.15 0.13 0.08 0.11 0.07 0.1 0.15 0.08 0.12 0.18 0.09 0 0 0 0.07 0 0.09 0.06 0.08 0.12
Endosome 0 0 0 0.15 0.19 0.15 0.11 0.08 0.08 0.07 0.06 0.05 0 0.09 0.18 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0.13 0.09 0.14 0.27 0.33 0.32 0.43 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.06 0 0.06 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.12 0.05 0.05 0 0 0.05
Nucleolus 0 0 0 0.07 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.15 0 0.23 0.38 0.25 0.11 0.24 0.27 0.19 0.14 0.16 0.18 0.1 0.05 0.08 0.15 0.26 0.12 0.05 0.12 0.09 0.09 0.06 0.08
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 3 4 5 4 9 4 7 4 5 1 0 4 0 0 0 0 0 0 0 1 0
Bud 0 0 3 2 0 6 4 9 7 8 4 10 1 0 2 0 0 0 0 1 2 0 3 1
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 2 1 2 0 1 4 2 3 4 4 0 0 1 2 2 0 0 0 0 0 0 0
Cytoplasm 115 108 213 96 166 124 201 191 112 151 87 118 109 155 146 106 54 102 100 97 251 48 168 152
Endoplasmic Reticulum 5 2 17 26 51 30 30 49 16 32 33 29 18 36 20 3 2 2 9 3 35 4 18 27
Endosome 0 0 1 35 67 37 42 37 20 23 13 18 5 19 41 0 2 0 0 1 7 2 4 4
Golgi 0 0 0 1 4 0 0 0 0 0 0 0 1 1 1 0 0 1 0 0 0 0 0 0
Mitochondria 0 0 2 3 2 31 34 61 65 109 71 158 1 0 2 1 0 1 0 1 2 0 0 0
Nucleus 1 0 0 1 0 0 4 4 2 1 4 3 1 0 1 1 1 5 7 8 14 4 7 2
Nuclear Periphery 0 0 0 1 3 3 5 5 3 0 5 8 1 0 0 0 0 0 17 7 20 3 8 11
Nucleolus 0 0 2 16 12 5 8 12 3 3 1 7 4 1 4 0 0 0 0 0 0 0 1 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 3 2 1 3 4 2 0 0 0 1 0 0 0 1 0 0 0 3 0 0 1
Vac/Vac Membrane 20 5 69 92 86 26 96 120 45 48 35 66 15 10 17 19 18 14 7 16 36 6 14 17
Unique Cell Count 135 113 297 240 345 239 395 439 241 334 224 367 146 203 222 123 69 121 146 140 383 75 234 226
Labelled Cell Count 141 115 309 280 399 268 432 505 281 386 261 426 158 222 239 132 80 125 146 140 383 75 234 226


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 23.0 17.3 12.2 11.6 11.1 9.1 8.6 8.2 7.8 7.6 7.4 7.2 18.9 17.3 14.7 18.1 20.9 20.2 16.2 20.3 21.8
Std Deviation (1e-4) 3.8 3.2 2.4 1.8 1.6 1.4 1.7 1.3 1.5 1.5 1.4 1.3 4.6 3.0 2.7 4.4 4.1 4.9 5.4 4.2 4.3
Intensity Change (Log2) – – – -0.07 -0.13 -0.41 -0.49 -0.58 -0.64 -0.69 -0.72 -0.77 0.64 0.51 0.27 0.57 0.78 0.73 0.41 0.74 0.84


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 1.3 0 1.6 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm -7.4 -6.1 -4.7 -5.5 -7.5 -6.0 -6.7 -7.5 -10.1 0.7 1.2 -1.5 3.2 1.1 2.7
Endoplasmic Reticulum 2.2 3.7 2.8 1.0 2.5 0.4 1.8 3.5 1.1 2.4 4.3 1.4 -1.4 0 -1.8
Endosome 6.6 7.8 6.8 5.6 4.9 4.7 4.3 3.8 3.5 0 5.1 7.5 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 5.9 4.7 6.3 9.2 10.5 10.1 12.7 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 3.8 2.4 0 0 2.0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 3.8 0.5 -3.7 0.3 1.2 -1.3 -2.9 -2.2 -1.7 -3.3 -5.5 -4.7 -1.8 0.5 -2.7

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 14.416 20.9874 17.2655 16.2258 14.5325 16.8432 13.4931 18.5162 16.7336 15.2569 14.8226 17.4307 18.4004 22.1027 20.044 19.016 19.004 20.1566
Actin 0.0253 0.0002 0.0068 0.0002 0.0256 0.0038 0.0274 0.0002 0.0053 0.0437 0.0021 0.0002 0.0061 0.0001 0.0096 0.0003 0.0078 0.0002
Bud 0.0002 0 0.0002 0.0001 0.0003 0.0002 0.0002 0.0001 0.0001 0.0017 0.0002 0 0.0001 0 0.0004 0 0.0001 0
Bud Neck 0.0003 0.0002 0.0006 0.0003 0.0009 0.0018 0.0004 0.0002 0.0004 0.0006 0.0007 0.0007 0.0002 0.0001 0.0018 0.0004 0.0003 0.0005
Bud Periphery 0.0002 0 0.0002 0 0.0008 0.0002 0.0002 0 0.0001 0.0024 0.0002 0 0.0001 0 0.0004 0 0.0001 0
Bud Site 0.0004 0.0001 0.0008 0.0001 0.0005 0.0007 0.0006 0.0004 0.0003 0.0058 0.0005 0 0.0005 0 0.0058 0.0001 0.0003 0
Cell Periphery 0.0001 0 0.0001 0 0.0002 0.0002 0.0001 0.0003 0 0.0002 0 0 0.0001 0 0.0001 0 0 0
Cytoplasm 0.2772 0.4118 0.3331 0.4483 0.2269 0.1835 0.4063 0.5344 0.5221 0.5383 0.3828 0.4667 0.3314 0.4237 0.4324 0.4721 0.5627 0.3147
Cytoplasmic Foci 0.0048 0.0008 0.0123 0.0009 0.0046 0.0023 0.0102 0.015 0.0121 0.0021 0.0092 0.0016 0.0142 0.0212 0.011 0.0085 0.0092 0.0054
Eisosomes 0.0003 0 0.0001 0 0.0005 0.0001 0.0002 0 0 0.0002 0 0 0 0 0.0001 0 0 0
Endoplasmic Reticulum 0.0222 0.01 0.0153 0.014 0.0168 0.0133 0.0459 0.0251 0.0245 0.0362 0.0297 0.021 0.0415 0.022 0.0312 0.0227 0.014 0.0256
Endosome 0.0077 0.0041 0.0139 0.0064 0.0235 0.0141 0.0297 0.0102 0.0123 0.0094 0.056 0.0044 0.0352 0.0054 0.0087 0.0255 0.0164 0.0194
Golgi 0.0032 0.0001 0.0038 0.0001 0.0204 0.0013 0.004 0.0005 0.0027 0.0079 0.0044 0 0.0066 0.0001 0.0038 0.0003 0.0058 0.0004
Lipid Particles 0.0096 0.0001 0.0081 0.0001 0.0381 0.0014 0.0111 0.0002 0.0027 0.0021 0.0042 0 0.0046 0.0002 0.0052 0.0014 0.0042 0.0006
Mitochondria 0.018 0.0002 0.003 0.0004 0.0386 0.0006 0.0018 0.0002 0.0007 0.0105 0.0035 0.0002 0.0018 0.0001 0.0011 0.0001 0.0038 0.0002
None 0.0013 0.0002 0.0008 0.0004 0.0005 0.0007 0.0067 0.0004 0.0009 0.0005 0.0023 0.0004 0.0011 0.0002 0.0005 0.0009 0.0005 0.0007
Nuclear Periphery 0.4341 0.3318 0.3803 0.3564 0.3163 0.4331 0.2965 0.2157 0.2553 0.177 0.2678 0.2721 0.3418 0.3375 0.326 0.2978 0.2109 0.3444
Nucleolus 0.0007 0.0001 0.0003 0.0001 0.0003 0.0005 0.0002 0.0003 0.0001 0.0001 0.0011 0 0.0004 0.0001 0.0001 0.0002 0.0001 0.0001
Nucleus 0.1775 0.2313 0.2052 0.1578 0.2719 0.3281 0.1467 0.1808 0.1458 0.1548 0.2113 0.2263 0.1961 0.1747 0.1487 0.1568 0.1433 0.2773
Peroxisomes 0.0007 0 0.0049 0 0.0029 0.0001 0.0017 0.0001 0.0042 0.0008 0.0022 0 0.0016 0 0.0076 0 0.0119 0.0001
Punctate Nuclear 0.0038 0.001 0.0034 0.001 0.0029 0.0022 0.0034 0.0111 0.0057 0.0015 0.0128 0.0023 0.0033 0.0096 0.0025 0.0039 0.0026 0.0043
Vacuole 0.0064 0.0046 0.0038 0.0079 0.0038 0.0074 0.0044 0.0032 0.003 0.0026 0.0047 0.0024 0.0085 0.0028 0.0016 0.0074 0.0035 0.0035
Vacuole Periphery 0.006 0.0034 0.0031 0.0055 0.0036 0.0045 0.0024 0.0014 0.0016 0.0014 0.0045 0.0017 0.0048 0.0021 0.0015 0.0015 0.0025 0.0025

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 134.6356 112.5132 95.3528 115.6379 112.874 97.1268 92.7938 85.5303 83.8122 119.1189
Translational Efficiency 0.7538 0.7897 0.8101 0.6465 0.692 0.8566 0.6993 0.5914 0.7057 0.6171

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✔
Gene Expression ✔
Translational Efficiency ✘
Standard name
Human Ortholog
Description Karyopherin/importin that interacts with the nuclear pore complex; acts as the nuclear import receptor for specific proteins, including Pdr1p, Yap1p, Ste12p, and Aft1p
Localization
Cell Percentages cytoplasm (42%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-6

Pse1

Pse1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Pse1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available