Standard name
Human Ortholog
Description Regulatory subunit of protein phosphatase 1 (Glc7p); involved in glycogen metabolism and chromosome segregation; proposed to regulate Glc7p activity via conformational alteration; ortholog of the mammalian protein phosphatase inhibitor 2; protein abundance increases in response to DNA replication stress
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.95 0.98 0.97 0.94 0.85 0.79 0.72 – 0.66 0.62 0.61 0.61 0.98 0.96 0.97 0.85 0.84 0.92 0.85 0.84 0.86 0.91 0.86 0.87
Endoplasmic Reticulum 0 0 0 0 0 0 0 – 0 0.05 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 – 0 0 0.1 0.06 0 0 0 0.05 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0.06 – 0 0.06 0.06 0.05 0 0 0 0 0 0 0.09 0.1 0.06 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.07 0 0 0.12 0.2 0.2 0.24 – 0.28 0.25 0.23 0.28 0 0.06 0.06 0.13 0.17 0.1 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 1 – 1 1 0 5 0 0 0 0 0 0 0 0 0 0 0 1
Bud 0 0 0 0 0 0 0 – 1 2 2 2 0 0 0 0 0 0 1 0 0 0 2 5
Bud Neck 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1
Bud Site 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 2 1 0 0 1 0 4 – 4 7 4 6 1 0 3 4 1 1 0 0 0 0 0 0
Cytoplasm 230 175 90 161 218 129 291 – 175 204 145 190 196 297 393 197 130 123 204 148 108 152 275 311
Endoplasmic Reticulum 0 1 1 1 3 0 8 – 3 16 5 12 1 0 3 11 2 3 0 0 0 0 2 8
Endosome 1 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 1 0 1 0 2 2
Golgi 2 0 1 0 0 1 1 – 0 0 2 1 0 2 0 3 0 1 2 0 0 0 3 2
Mitochondria 1 0 0 0 0 6 3 – 10 12 23 19 0 0 0 11 4 2 0 0 1 0 1 0
Nucleus 8 3 0 4 10 3 25 – 11 18 13 16 2 1 4 3 3 2 22 17 7 5 8 8
Nuclear Periphery 0 0 0 0 0 0 0 – 0 0 0 1 0 0 0 0 0 0 0 0 2 0 1 4
Nucleolus 0 0 0 0 0 0 0 – 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 17 2 3 21 52 32 96 – 75 83 55 87 6 20 24 29 26 13 4 7 2 4 12 8
Unique Cell Count 241 178 93 172 257 163 402 267 327 236 312 200 309 406 231 155 134 240 176 126 167 319 360
Labelled Cell Count 261 182 95 187 284 171 429 280 344 249 339 206 320 427 258 166 145 240 176 126 167 319 360


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 16.8 16.1 13.0 13.9 15.8 10.2 13.6 – 11.9 12.3 11.3 12.0 18.9 17.0 20.4 22.6 24.1 22.8 15.4 16.4 16.7
Std Deviation (1e-4) 2.8 2.6 2.5 2.0 2.4 2.4 2.3 – 2.5 2.5 2.4 2.7 3.2 2.9 3.5 7.8 9.1 7.5 2.9 3.0 3.4
Intensity Change (Log2) – – – 0.1 0.28 -0.34 0.07 – -0.13 -0.08 -0.2 -0.12 0.54 0.39 0.65 0.8 0.89 0.81 0.25 0.34 0.37


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Cytoplasm -1.1 -3.0 -3.9 -5.0 – -5.9 -6.4 -6.4 -6.6 0.6 -0.3 0 -2.9 -3.1 -1.5
Endoplasmic Reticulum 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 – 0 0 3.1 0 0 0 0 0 0 0
Nucleus 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Vacuole 2.4 3.9 3.7 4.5 – 5.0 4.7 4.3 5.0 0 1.2 1.0 2.5 3.2 1.9

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 12.0942 13.0767 11.944 10.9846 10.594 11.9254 13.5035 15.957 14.9067 12.756 10.9713 14.2491 16.4997 18.1255 16.7658 16.5169 16.6524 16.2864
Actin 0.0049 0.0001 0.0002 0.0002 0.0001 0.0006 0.0102 0.0074 0.0045 0.0018 0.0068 0.0023 0.0102 0.0001 0.0002 0.0001 0.0002 0.0019
Bud 0.0001 0.0001 0.0001 0.0001 0.0001 0.0005 0.0009 0.005 0.0053 0.0003 0.0005 0.0004 0.0002 0.0001 0.0001 0 0.0001 0.0001
Bud Neck 0.0006 0.0002 0.0003 0.0004 0.0005 0.0009 0.0018 0.0007 0.0007 0.001 0.0012 0.0014 0.0004 0.0002 0.0004 0.0004 0.0007 0.0011
Bud Periphery 0.0001 0 0 0 0 0.0005 0.001 0.001 0.0011 0.0002 0.0004 0.0001 0.0002 0 0 0 0 0.0001
Bud Site 0.0005 0.0001 0.0001 0 0 0.0001 0.0068 0.0017 0.0047 0.0004 0.0013 0.0002 0.0005 0.0001 0.0002 0 0 0.0002
Cell Periphery 0.0001 0 0 0 0 0 0.0002 0.0001 0.0001 0.0001 0.0001 0 0.0001 0 0 0 0 0
Cytoplasm 0.6828 0.7455 0.7175 0.6132 0.6756 0.5905 0.589 0.7824 0.7108 0.6939 0.5196 0.6469 0.6977 0.7683 0.8215 0.6947 0.779 0.6207
Cytoplasmic Foci 0.0045 0.0002 0.0008 0.0003 0.0005 0.0005 0.0095 0.0009 0.0043 0.0056 0.0788 0.0093 0.0024 0.0003 0.0006 0.0004 0.0005 0.0038
Eisosomes 0 0 0 0 0 0 0.0001 0 0 0 0 0 0 0 0 0 0 0
Endoplasmic Reticulum 0.0027 0.0012 0.0009 0.0004 0.0011 0.0028 0.0036 0.0012 0.0033 0.0101 0.0019 0.0011 0.0059 0.0005 0.0014 0.0006 0.0009 0.0019
Endosome 0.0089 0.0002 0.0005 0.0002 0.0005 0.0012 0.0352 0.0004 0.0067 0.0237 0.0832 0.0106 0.005 0.0001 0.0013 0.0001 0.0003 0.0035
Golgi 0.0019 0 0 0 0 0.0001 0.0067 0.0002 0.0021 0.0016 0.05 0.0094 0.0005 0 0 0 0 0.001
Lipid Particles 0.0061 0 0 0 0 0 0.0097 0 0.0014 0.0019 0.0234 0.0005 0.0014 0 0 0 0 0.0043
Mitochondria 0.0009 0.0001 0.0002 0.0002 0.0002 0.0023 0.0047 0.0007 0.0014 0.0007 0.0087 0.0056 0.0017 0.0001 0.0002 0.0001 0.0002 0.0017
None 0.0014 0.0003 0.0004 0.0003 0.0004 0.0003 0.0039 0.0005 0.0017 0.0013 0.0005 0.0011 0.0019 0.0003 0.0005 0.0003 0.0005 0.0011
Nuclear Periphery 0.0293 0.0135 0.0211 0.0149 0.0212 0.0266 0.025 0.0102 0.016 0.0205 0.0143 0.0151 0.0185 0.0086 0.0075 0.0072 0.0125 0.0173
Nucleolus 0.0003 0.0001 0.0001 0.0001 0.0001 0.0001 0.0006 0.0007 0.0003 0.0002 0.0002 0.0002 0.0012 0 0.0001 0 0.0001 0.0002
Nucleus 0.2487 0.2372 0.256 0.3681 0.2956 0.3699 0.2716 0.1842 0.2298 0.2281 0.1567 0.2842 0.2465 0.2202 0.1633 0.2951 0.2035 0.3354
Peroxisomes 0.0017 0 0 0 0 0 0.0018 0.0002 0.0006 0.0003 0.0462 0.0008 0.0006 0 0 0 0 0.0023
Punctate Nuclear 0.0029 0.0009 0.0011 0.0012 0.0025 0.001 0.0072 0.0018 0.0028 0.0052 0.0032 0.0095 0.0035 0.0007 0.001 0.0007 0.001 0.0026
Vacuole 0.0012 0.0003 0.0006 0.0003 0.0012 0.0015 0.0077 0.0006 0.0017 0.0023 0.0022 0.0009 0.0012 0.0002 0.0013 0.0001 0.0004 0.0006
Vacuole Periphery 0.0005 0.0001 0.0002 0.0001 0.0003 0.0006 0.0027 0.0002 0.0006 0.0007 0.0008 0.0003 0.0004 0 0.0002 0 0.0001 0.0003

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 31.9827 30.0201 28.6409 38.5031 43.5404 54.3406 39.7484 43.4118 55.7603 44.9746
Translational Efficiency 4.2204 4.2083 3.3818 3.3379 3.1344 2.9136 2.6294 2.9782 2.3516 2.7598

Hit Data

Dataset Hit
Protein Concentration ✔
Protein Localization ✔
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Regulatory subunit of protein phosphatase 1 (Glc7p); involved in glycogen metabolism and chromosome segregation; proposed to regulate Glc7p activity via conformational alteration; ortholog of the mammalian protein phosphatase inhibitor 2; protein abundance increases in response to DNA replication stress
Localization
Cell Percentages cytoplasm (99%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

Glc8

Glc8


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Glc8-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available