Standard name
Human Ortholog
Description Bifunctional triacylglycerol lipase and LPE acyltransferase; major lipid particle-localized triacylglycerol (TAG) lipase; catalyzes acylation of lysophosphatidylethanolamine (LPE), a function which is essential for sporulation; protein level and stability of Tgl3p are markedly reduced in the absence of lipid droplets; required with Tgl4p for timely bud formation
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0.05 0 0.05 0 0 0 0 0 0 0.06 0.05 0.05 0.06 0.06 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0.17 0.19 0.27 0.15 0.16 0.18 0.15 0.21 0.16 0.23 0.06 0.18 0.26 0.26 0.38 0.2 0.21 0.24 0.32 0.33 0.41 0.29 0.34 0.36
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.38 0.39 0.11 0.11 0.05 0.14 0.2 0.23 0.37 0.32 0.6 0.52 0.17 0.09 0 0.11 0.07 0.06 0 0.06 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.22 0.17 0.13 0.13 0.12 0.19 0.2 0.25 0.3 0.29 0.33 0.31 0.13 0.16 0.2 0.31 0.31 0.28 0 0 0 0 0 0
Peroxisomes 0.45 0.42 0.35 0.54 0.4 0.42 0.27 0.22 0.21 0.15 0.09 0.09 0.55 0.5 0.49 0.45 0.44 0.35 0.36 0.32 0.31 0.33 0.34 0.37
SpindlePole 0.35 0.2 0.45 0.52 0.55 0.53 0.5 0.44 0.34 0.31 0.18 0.21 0.47 0.44 0.46 0.37 0.35 0.41 0.22 0.12 0.14 0.17 0.17 0.12
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 1 2 0 3 0 0 1 2 1 0 0 0 1 0 0 0 0 0 1 9 1 0 1 2
Bud 3 7 1 5 6 5 10 17 9 6 13 3 2 0 1 0 2 2 2 5 3 4 2 2
Bud Neck 0 0 0 0 0 1 3 1 1 1 1 0 2 1 2 0 0 1 0 0 1 0 0 0
Bud Site 5 3 0 1 0 1 1 3 2 0 6 6 0 0 0 0 1 2 – – – – – –
Cell Periphery 0 0 0 0 1 0 1 1 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0
Cytoplasm 2 4 8 7 14 9 19 23 8 8 7 11 8 13 13 10 16 15 0 0 0 3 0 1
Endoplasmic Reticulum 0 1 0 0 0 0 1 1 0 1 1 0 0 0 0 1 5 1 0 0 1 0 0 1
Endosome 35 55 59 40 50 60 63 112 43 67 21 65 51 57 102 40 59 60 75 107 104 55 91 97
Golgi 0 0 1 0 0 0 0 0 0 0 0 0 1 0 1 0 3 2 5 14 4 7 6 6
Mitochondria 80 115 24 30 14 46 83 123 99 93 206 186 33 20 11 22 19 14 3 21 11 7 11 11
Nucleus 0 2 1 1 2 5 7 8 5 10 8 4 0 0 0 2 2 1 0 0 0 1 1 1
Nuclear Periphery 1 5 0 1 1 0 0 8 1 7 6 14 0 0 0 2 0 3 1 1 0 1 0 1
Nucleolus 46 51 29 34 36 63 85 136 80 84 115 110 26 34 55 61 90 70 0 6 2 2 2 1
Peroxisomes 96 123 78 145 123 140 111 120 57 43 32 31 108 108 133 89 125 89 84 105 79 63 91 100
SpindlePole 74 60 98 140 170 176 208 238 93 90 62 76 93 96 125 73 101 104 51 39 36 31 47 33
Vac/Vac Membrane 0 6 4 1 2 2 2 3 2 5 4 7 1 2 3 7 5 11 0 4 0 3 3 2
Unique Cell Count 212 293 220 268 309 334 416 546 270 288 346 359 198 216 271 196 287 252 235 327 255 190 271 272
Labelled Cell Count 343 434 303 408 419 508 595 796 401 415 482 513 326 331 447 307 428 375 235 327 255 190 271 272


Lipid Particles, Punctate Nuclear

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 7.0 9.0 9.2 8.2 8.5 7.2 7.0 7.4 6.7 7.0 6.3 6.8 8.4 9.0 8.7 12.1 12.4 11.5 8.8 8.4 8.5
Std Deviation (1e-4) 2.0 2.7 3.7 2.3 3.1 2.2 3.1 2.3 2.4 2.3 2.1 2.3 2.1 2.9 2.2 4.5 4.1 4.2 2.5 2.3 2.2
Intensity Change (Log2) – – – -0.17 -0.12 -0.35 -0.39 -0.31 -0.46 -0.39 -0.54 -0.44 -0.13 -0.03 -0.08 0.4 0.43 0.32 -0.07 -0.13 -0.12


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 2.2 0 0 2.5 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm -0.7 0.5 -0.6 0.6 0.4 -0.4 -0.5 -1.2 -0.4 0.2 1.2 0.6 0.7 1.0 1.2
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome -3.3 -3.0 -2.5 -3.6 -1.9 -3.0 -0.9 -6.9 -2.5 -0.2 -0.1 2.5 -1.5 -1.7 -0.8
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.1 -2.8 1.0 2.9 3.7 6.5 5.7 11.5 9.9 1.7 -0.6 -2.9 0.1 -1.7 -2.1
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 3.0 0 0 0 0 0 0
Nucleolus -0.2 -0.5 1.8 2.3 3.6 4.4 4.3 5.3 4.8 0 0.8 2.1 4.4 4.8 3.9
Peroxisomes 4.1 1.0 1.5 -2.3 -3.9 -3.5 -5.4 -7.7 -8.0 3.9 3.1 3.0 2.1 1.8 0
SpindlePole 1.7 2.4 1.9 1.3 -0.2 -2.3 -3.1 -6.9 -6.0 0.5 0 0.3 -1.5 -2.1 -0.7
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 1.1 0 1.6

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 2.4169 4.3956 3.6772 4.0532 3.749 3.9161 2.0489 4.4025 3.6749 3.3919 3.0332 4.0507 2.6401 6.0794 4.7395 3.8203 4.9467 4.4277
Actin 0.0022 0.0011 0.0041 0.0001 0.0002 0.0008 0.0085 0.0009 0.004 0.0352 0.0001 0.0001 0.0102 0.0003 0.0019 0 0.0055 0.0006
Bud 0.0002 0.0016 0.0006 0 0 0.0001 0.0001 0 0.0001 0.0011 0.0001 0 0.0001 0 0 0 0.0002 0
Bud Neck 0.0039 0.0008 0.0009 0.0001 0.0002 0.0002 0.0003 0.0003 0.0002 0.0008 0.0005 0.0001 0.0006 0.0002 0.0001 0 0.0005 0.0001
Bud Periphery 0.0002 0.0028 0.0009 0 0 0.0001 0.0002 0 0.0002 0.0025 0.0001 0 0.0001 0.0001 0 0 0.0003 0
Bud Site 0.0025 0.0043 0.0049 0.0001 0.0002 0.0003 0.0005 0.0003 0.0005 0.0145 0.0011 0 0.0004 0.0003 0.0001 0 0.0016 0.0001
Cell Periphery 0.0002 0.0005 0.0003 0.0001 0.0001 0.0001 0.0002 0.0001 0.0001 0.0006 0.0001 0 0.0001 0.0002 0 0 0.0003 0
Cytoplasm 0.0016 0.0005 0.0017 0.0009 0.0002 0.0002 0.0007 0.0001 0.0003 0.0013 0.0005 0.0001 0.001 0 0.0004 0 0.0022 0
Cytoplasmic Foci 0.2613 0.2688 0.2587 0.2616 0.2497 0.2079 0.1934 0.2216 0.2059 0.2027 0.2124 0.1792 0.1731 0.1917 0.2073 0.2 0.2581 0.1408
Eisosomes 0 0 0.0001 0 0 0 0.0001 0 0 0.0004 0 0 0.0001 0 0 0 0.0001 0
Endoplasmic Reticulum 0.0011 0.0001 0.0007 0.0004 0.0001 0.0002 0.0006 0.0001 0.0002 0.0014 0.0001 0.0001 0.0006 0 0.0008 0 0.0049 0
Endosome 0.0265 0.0297 0.0257 0.0364 0.0224 0.0128 0.0332 0.0121 0.0178 0.0174 0.0371 0.0152 0.0101 0.0064 0.0055 0.0131 0.019 0.002
Golgi 0.0109 0.0271 0.0217 0.0213 0.019 0.0155 0.0187 0.0157 0.0168 0.0209 0.0153 0.0097 0.0086 0.0111 0.0117 0.0122 0.0071 0.0064
Lipid Particles 0.565 0.5303 0.5012 0.5645 0.6269 0.6823 0.6172 0.6505 0.6386 0.5783 0.4805 0.7369 0.6616 0.6741 0.6796 0.7056 0.6011 0.8155
Mitochondria 0.0117 0.0026 0.0176 0.0012 0.0013 0.0109 0.0075 0.0032 0.004 0.0186 0.0088 0.0018 0.0026 0.011 0.0059 0.0014 0.0028 0.0021
None 0.0009 0.0001 0.0052 0.0005 0 0.0001 0.0013 0 0.0001 0.0032 0.0001 0 0.0013 0 0.0005 0 0.0014 0
Nuclear Periphery 0.0029 0.0007 0.0009 0.0011 0.0001 0.0003 0.0021 0.0018 0.0009 0.0078 0.0006 0.0034 0.0022 0.0001 0.0072 0.0001 0.0206 0.0007
Nucleolus 0.016 0.0416 0.0191 0.0129 0.0288 0.0018 0.0358 0.0271 0.0101 0.0088 0.0726 0.0081 0.025 0.0451 0.0076 0.0005 0.0115 0.0058
Nucleus 0.0021 0.0022 0.0009 0.0004 0.0005 0.0002 0.002 0.0007 0.0005 0.0075 0.0017 0.0004 0.0017 0.0016 0.0011 0 0.0095 0.0003
Peroxisomes 0.0443 0.0432 0.113 0.0708 0.047 0.0492 0.0385 0.041 0.0697 0.0615 0.0275 0.0273 0.0478 0.0432 0.0481 0.0648 0.025 0.0176
Punctate Nuclear 0.0385 0.0244 0.0174 0.0251 0.0018 0.0137 0.0222 0.0194 0.0268 0.0093 0.1347 0.0124 0.0504 0.0082 0.0209 0.0012 0.0137 0.0066
Vacuole 0.006 0.0113 0.0027 0.0013 0.0009 0.0024 0.0108 0.0014 0.0014 0.0044 0.0037 0.0014 0.0012 0.0036 0.0004 0.0003 0.0099 0.0004
Vacuole Periphery 0.0021 0.0062 0.0019 0.0012 0.0005 0.001 0.0061 0.0037 0.0016 0.0018 0.0022 0.0037 0.0013 0.0026 0.0008 0.0004 0.0048 0.0008

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 12.5842 10.2268 11.7625 18.7573 14.091 13.1489 17.7078 23.3797 19.1257 21.9262
Translational Efficiency 0.644 1.0246 0.8149 0.5249 0.5835 0.6027 0.6882 0.4521 0.6555 0.5609

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Bifunctional triacylglycerol lipase and LPE acyltransferase; major lipid particle-localized triacylglycerol (TAG) lipase; catalyzes acylation of lysophosphatidylethanolamine (LPE), a function which is essential for sporulation; protein level and stability of Tgl3p are markedly reduced in the absence of lipid droplets; required with Tgl4p for timely bud formation
Localization
Cell Percentages —
Cell Cycle Regulation No
Subcompartmental Group N/A

Tgl3

Tgl3


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Tgl3-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available