ORF
Human Ortholog
Description Protein with NADP(H) oxidoreductase activity; transcription is regulated by Stb5p in response to NADPH depletion induced by diamide; promoter contains a putative Stb5p binding site; protein abundance increases in response to DNA replication stress
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0.08
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.98 1.0 0.97 0.96 0.94 0.92 0.87 0.75 0.84 0.8 0.86 0.72 0.99 0.98 1.0 0.97 0.99 0.96 0.91 0.9 0.89 0.93 0.84 0.86
Endoplasmic Reticulum 0 0 0 0 0 0 0.05 0.07 0.06 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0.06 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0.06 0 0.05 0.16 0.05 0.13 0.07 0.2 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 2 3 0 1 1 4 0 0 0 0 0 0 0 0 1 0 0 0
Bud 0 0 0 0 0 0 2 5 2 3 2 0 0 0 0 0 0 0 5 5 0 2 14 21
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 2 0 2 4 7 2 9 7 4 6 7 14 1 5 2 7 1 6 0 0 0 0 2 0
Cytoplasm 197 140 115 173 261 192 270 269 178 228 195 199 175 193 220 156 175 161 190 131 116 110 198 232
Endoplasmic Reticulum 0 0 0 2 2 5 14 26 12 9 6 8 1 1 1 2 8 4 0 0 2 0 3 1
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 1 0 2 0 0 1
Golgi 0 0 0 0 0 1 1 2 0 0 0 3 0 1 1 0 0 1 1 0 0 0 0 0
Mitochondria 5 0 0 1 1 1 7 6 12 10 12 11 0 1 0 0 0 2 1 1 1 0 1 1
Nucleus 0 0 0 0 0 5 9 6 1 4 4 1 1 0 0 0 0 0 0 0 0 1 6 3
Nuclear Periphery 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1
Nucleolus 1 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 2 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0
SpindlePole 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2 0 0 0 2 1
Vac/Vac Membrane 1 0 3 5 17 2 17 58 10 38 15 55 0 0 0 3 3 5 1 1 2 0 1 1
Unique Cell Count 201 140 119 181 279 208 311 361 211 284 228 275 177 197 221 160 177 168 209 145 131 119 236 270
Labelled Cell Count 207 140 121 185 288 209 331 382 219 299 242 295 178 202 224 168 187 181 209 145 131 119 236 270


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 7.3 15.4 11.7 10.4 13.1 13.9 15.4 16.1 15.3 16.0 15.7 17.5 16.9 28.3 33.2 38.5 39.9 37.7 13.7 14.2 16.6
Std Deviation (1e-4) 2.9 6.1 3.9 3.6 4.2 4.0 4.7 4.3 4.7 4.6 4.7 5.2 6.4 10.3 9.5 13.2 12.3 14.7 9.0 7.8 7.1
Intensity Change (Log2) – – – -0.17 0.16 0.25 0.4 0.46 0.39 0.45 0.42 0.58 0.53 1.27 1.51 1.72 1.77 1.69 0.23 0.28 0.5


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm -0.5 -1.2 -1.6 -3.0 -5.2 -3.4 -4.2 -3.2 -5.5 1.3 0.7 2.1 0.4 1.3 -0.4
Endoplasmic Reticulum 0 0 0 0 3.0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 1.5 0 1.3 3.8 0 3.3 1.6 4.5 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 12.7169 13.9753 12.8072 11.9057 14.0232 12.3 16.3527 16.8978 15.989 15.0159 15.4231 16.4713 12.3629 13.9974 12.6475 13.144 13.7179 15.249
Actin 0.0098 0.0014 0.0074 0.0004 0.0009 0.011 0.0012 0.0029 0.0015 0.0179 0.0029 0.0012 0.0132 0.0014 0.0046 0.0126 0.0044 0.0005
Bud 0.0007 0.0065 0.0012 0.0002 0.0025 0.0009 0.0015 0.002 0.0025 0.0066 0.0078 0.0009 0.0006 0.0011 0.0017 0.0014 0.0044 0.0002
Bud Neck 0.0007 0.0002 0.0006 0.0006 0.0005 0.0014 0.0004 0.0004 0.0006 0.0012 0.0008 0.0013 0.0005 0.0001 0.0005 0.0006 0.0011 0.0006
Bud Periphery 0.0004 0.0024 0.0005 0.0001 0.001 0.0006 0.0011 0.0009 0.002 0.0049 0.0052 0.0006 0.0005 0.0002 0.0013 0.0013 0.0027 0.0001
Bud Site 0.0019 0.0017 0.0025 0.0006 0.0002 0.0018 0.0011 0.0053 0.0024 0.0016 0.0005 0.0002 0.0029 0.003 0.008 0.011 0.0043 0
Cell Periphery 0.0002 0.0001 0.0001 0.0001 0 0 0.0001 0.0002 0.0001 0.0002 0.0001 0 0.0001 0.0001 0.0002 0.0002 0.0001 0
Cytoplasm 0.8827 0.9736 0.9241 0.9108 0.9785 0.9314 0.9493 0.9699 0.9487 0.9232 0.9308 0.9619 0.8519 0.9877 0.9073 0.8989 0.8765 0.9611
Cytoplasmic Foci 0.0217 0.0015 0.01 0.0254 0.0019 0.0107 0.0029 0.0025 0.0028 0.0041 0.0054 0.0037 0.0322 0.001 0.0108 0.0217 0.0293 0.0014
Eisosomes 0.0002 0 0.0001 0 0 0 0 0 0 0 0 0 0.0001 0 0 0.0001 0 0
Endoplasmic Reticulum 0.0042 0.0009 0.0021 0.002 0.0008 0.0027 0.0036 0.0011 0.0021 0.0031 0.0037 0.0026 0.0044 0.0006 0.0052 0.0019 0.0022 0.0014
Endosome 0.0146 0.0006 0.01 0.0122 0.0005 0.0061 0.0015 0.0009 0.0014 0.0017 0.0059 0.0017 0.0383 0.0003 0.0101 0.0161 0.053 0.0162
Golgi 0.0064 0.0001 0.0039 0.0022 0.0001 0.002 0.0001 0.0002 0.0002 0.0003 0.0009 0.0001 0.0094 0 0.0039 0.005 0.003 0.0029
Lipid Particles 0.0113 0 0.0059 0.0084 0 0.0018 0.0001 0 0 0.0001 0.0001 0 0.0042 0 0.0014 0.0019 0.0011 0.0002
Mitochondria 0.006 0.0001 0.0047 0.0023 0.0004 0.001 0.0003 0.0002 0.0003 0.0004 0.0034 0.0002 0.0066 0.0001 0.0117 0.0037 0.0021 0.0026
None 0.0068 0.0057 0.0085 0.0012 0.0025 0.002 0.0058 0.0017 0.005 0.0062 0.0024 0.0023 0.0036 0.0007 0.0143 0.0016 0.0012 0.0006
Nuclear Periphery 0.0046 0.001 0.0016 0.0048 0.0015 0.0066 0.0041 0.0015 0.0036 0.0038 0.0048 0.0031 0.0054 0.0008 0.0031 0.0031 0.0023 0.0022
Nucleolus 0.0003 0 0.0001 0.0007 0 0.0001 0.0001 0 0.0001 0.0001 0 0 0.0002 0 0.0002 0.0005 0.0001 0
Nucleus 0.0119 0.0034 0.0059 0.0138 0.0078 0.0114 0.0237 0.0087 0.0228 0.016 0.0209 0.0171 0.0093 0.0022 0.0039 0.0073 0.0067 0.008
Peroxisomes 0.0097 0.0001 0.0082 0.0052 0.0001 0.0051 0.0001 0.0001 0.0002 0.001 0.0001 0.0001 0.0069 0 0.007 0.0065 0.0018 0
Punctate Nuclear 0.004 0.0004 0.0012 0.0025 0.0005 0.0018 0.0009 0.0006 0.0018 0.0023 0.0016 0.0012 0.0041 0.0002 0.0004 0.0023 0.0017 0.0002
Vacuole 0.0013 0.0003 0.0011 0.0048 0.0003 0.0012 0.0017 0.0008 0.0016 0.0047 0.0021 0.0013 0.0041 0.0002 0.0028 0.0016 0.0016 0.0008
Vacuole Periphery 0.0004 0.0001 0.0003 0.0016 0.0001 0.0005 0.0003 0.0001 0.0003 0.0004 0.0004 0.0002 0.0014 0.0001 0.0015 0.0006 0.0005 0.001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 160.2564 94.88 74.5875 94.4106 75.6181 123.933 124.9029 124.6887 132.0134 116.8221
Translational Efficiency 1.5862 1.9243 1.7926 1.8292 1.8204 2.5974 1.6691 1.6397 1.7908 1.8597

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
ORF
Human Ortholog
Description Protein with NADP(H) oxidoreductase activity; transcription is regulated by Stb5p in response to NADPH depletion induced by diamide; promoter contains a putative Stb5p binding site; protein abundance increases in response to DNA replication stress
Localization
Cell Percentages cytoplasm (86%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

YMR315W

YMR315W


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Ymr315w-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available