Standard name | |
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Human Ortholog | |
Description | Low-affinity Fe(II) transporter of the plasma membrane |
ensLOC | DeepLoc | |||||||||||||||||||||||
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Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 3 | 1 | 2 | 1 | 0 | 0 | 0 | 2 | 0 | 1 | 0 | 0 | 31 | 30 | 51 | – | – | – | 9 | 3 | 25 | 21 | 19 | 13 |
Bud | 2 | 1 | 2 | 4 | 0 | 10 | 3 | 7 | 8 | 16 | 15 | 8 | 20 | 20 | 15 | – | – | – | 4 | 1 | 7 | 8 | 9 | 13 |
Bud Neck | 0 | 0 | 2 | 0 | 1 | 0 | 3 | 6 | 2 | 4 | 1 | 1 | 14 | 13 | 12 | – | – | – | 12 | 3 | 12 | 17 | 12 | 11 |
Bud Site | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 2 | 0 | 1 | 2 | 3 | 6 | – | – | – | – | – | – | – | – | – |
Cell Periphery | 1 | 2 | 5 | 3 | 3 | 4 | 1 | 3 | 4 | 1 | 4 | 0 | 58 | 58 | 70 | – | – | – | 3 | 1 | 9 | 19 | 22 | 13 |
Cytoplasm | 288 | 121 | 256 | 175 | 275 | 347 | 412 | 414 | 351 | 397 | 272 | 241 | 679 | 679 | 634 | – | – | – | 215 | 103 | 119 | 74 | 160 | 172 |
Endoplasmic Reticulum | 0 | 2 | 2 | 1 | 0 | 3 | 1 | 0 | 1 | 0 | 0 | 2 | 12 | 11 | 17 | – | – | – | 5 | 0 | 16 | 11 | 12 | 18 |
Endosome | 0 | 0 | 0 | 0 | 2 | 2 | 3 | 5 | 2 | 3 | 0 | 1 | 1 | 1 | 1 | – | – | – | 3 | 1 | 4 | 1 | 2 | 9 |
Golgi | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | 0 | 0 | 1 | 1 | 1 | 3 |
Mitochondria | 3 | 5 | 9 | 7 | 8 | 49 | 104 | 123 | 207 | 231 | 195 | 229 | 0 | 0 | 1 | – | – | – | 12 | 7 | 37 | 27 | 26 | 19 |
Nucleus | 0 | 0 | 0 | 1 | 1 | 1 | 4 | 2 | 2 | 2 | 1 | 3 | 6 | 6 | 4 | – | – | – | 0 | 0 | 0 | 0 | 0 | 1 |
Nuclear Periphery | 0 | 1 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 0 | – | – | – | 0 | 0 | 0 | 0 | 0 | 1 |
Nucleolus | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | – | – | – | 0 | 0 | 0 | 0 | 1 | 0 |
Peroxisomes | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 1 | 2 | 0 | 1 | 4 | 0 | 0 | 0 | – | – | – | 0 | 0 | 1 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 1 | 1 | 7 | 4 | 6 | 9 | 15 | 10 | 11 | 10 | 17 | – | – | – | 0 | 0 | 1 | 1 | 3 | 3 |
Vac/Vac Membrane | 1 | 0 | 1 | 2 | 5 | 7 | 11 | 4 | 3 | 7 | 6 | 7 | 3 | 4 | 15 | – | – | – | 28 | 13 | 23 | 30 | 51 | 78 |
Unique Cell Count | 292 | 128 | 271 | 183 | 286 | 378 | 474 | 488 | 494 | 535 | 394 | 422 | 771 | 769 | 760 | 300 | 140 | 271 | 220 | 332 | 365 | |||
Labelled Cell Count | 298 | 134 | 284 | 195 | 297 | 424 | 549 | 571 | 590 | 674 | 510 | 509 | 837 | 835 | 843 | 300 | 140 | 271 | 220 | 332 | 365 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 3.8 | 3.6 | 3.7 | 4.2 | 3.9 | 3.2 | 3.0 | 3.1 | 2.7 | 2.5 | 2.6 | 2.6 | 5.6 | 5.6 | 6.8 | 3.6 | 4.2 | 4.8 | 5.7 | 5.8 | 6.2 |
Std Deviation (1e-4) | 0.7 | 0.6 | 1.3 | 1.9 | 1.6 | 1.4 | 1.4 | 1.2 | 1.0 | 1.2 | 1.3 | 0.6 | 1.4 | 1.4 | 2.5 | 0.5 | 0.8 | 1.2 | 1.9 | 1.7 | 2.4 |
Intensity Change (Log2) | – | – | – | 0.21 | 0.11 | -0.21 | -0.29 | -0.23 | -0.42 | -0.54 | -0.47 | -0.52 | 0.61 | 0.61 | 0.88 | -0.03 | 0.19 | 0.38 | 0.64 | 0.66 | 0.75 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Actin | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud Neck | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud Site | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cell Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cyto | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endoplasmic Reticulum | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endosome | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Golgi | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Mitochondria | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nuclear Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nuc | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleolus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Peroxisomes | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
SpindlePole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Vac | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cortical Patches | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cytoplasm | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Vacuole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 0.818 | 0.0395 | 0.4212 | -0.2335 | 0.6888 | 0.4531 | 0.6104 | 0.5149 | 0.7015 | 0.0942 | 0.4144 | 0.5455 | -0.8526 | 0.3151 | 0.3064 | 0.327 | 0.1195 | 0.0422 |
Actin | 0.0293 | 0.0026 | 0.0107 | 0.0007 | 0.0623 | 0.0103 | 0.0046 | 0.0008 | 0.0062 | 0.001 | 0.0031 | 0.0034 | 0.0002 | 0.0002 | 0.0002 | 0.001 | 0 | 0 |
Bud | 0.0006 | 0.0005 | 0.0017 | 0.0006 | 0.006 | 0.0016 | 0.0003 | 0.0001 | 0.0069 | 0.003 | 0.0002 | 0.0006 | 0 | 0.0002 | 0 | 0.001 | 0 | 0 |
Bud Neck | 0.0051 | 0.0002 | 0.0005 | 0.0008 | 0.002 | 0.0161 | 0.001 | 0.0003 | 0.0018 | 0.0003 | 0.0015 | 0.0021 | 0 | 0.0005 | 0.0001 | 0.0089 | 0 | 0.0008 |
Bud Periphery | 0.0009 | 0.0017 | 0.0025 | 0.0015 | 0.0237 | 0.0068 | 0.0004 | 0.0005 | 0.0131 | 0.0103 | 0.0011 | 0.001 | 0 | 0.0001 | 0 | 0.0066 | 0 | 0 |
Bud Site | 0.0125 | 0.0029 | 0.0034 | 0.0008 | 0.0031 | 0.0007 | 0.0041 | 0.0016 | 0.0099 | 0.0023 | 0.0003 | 0.0022 | 0.0001 | 0.0075 | 0.0001 | 0.0037 | 0 | 0 |
Cell Periphery | 0.0041 | 0.0008 | 0.0007 | 0.0008 | 0.0064 | 0.0004 | 0.0012 | 0.0104 | 0.0082 | 0.002 | 0.0253 | 0.0004 | 0.0003 | 0.0002 | 0.0002 | 0.0246 | 0 | 0.0002 |
Cytoplasm | 0.0805 | 0.0979 | 0.0992 | 0.1205 | 0.073 | 0.0925 | 0.1203 | 0.3203 | 0.2097 | 0.3218 | 0.4126 | 0.2939 | 0.0425 | 0.03 | 0.0949 | 0.3081 | 0.0237 | 0.1839 |
Cytoplasmic Foci | 0.0585 | 0.0063 | 0.0092 | 0.0276 | 0.0356 | 0.0128 | 0.0336 | 0.0108 | 0.0166 | 0.0075 | 0.0172 | 0.0166 | 0.0116 | 0.0021 | 0.0015 | 0.0083 | 0.0008 | 0.0025 |
Eisosomes | 0.0004 | 0.0003 | 0.0005 | 0.0002 | 0.0006 | 0.0002 | 0.0003 | 0.0002 | 0.0005 | 0.0001 | 0.0003 | 0.0001 | 0.0005 | 0.0005 | 0.0002 | 0.0003 | 0.0001 | 0.0001 |
Endoplasmic Reticulum | 0.0081 | 0.0008 | 0.0016 | 0.0015 | 0.0048 | 0.0015 | 0.0053 | 0.0053 | 0.0028 | 0.0031 | 0.0028 | 0.0017 | 0.0015 | 0.0007 | 0.0012 | 0.0027 | 0.0001 | 0.0005 |
Endosome | 0.0486 | 0.0024 | 0.0028 | 0.0049 | 0.0551 | 0.0194 | 0.0255 | 0.0035 | 0.0102 | 0.0576 | 0.0063 | 0.0139 | 0.0017 | 0.0019 | 0.0012 | 0.0014 | 0 | 0.0011 |
Golgi | 0.0117 | 0.0002 | 0.0014 | 0.0004 | 0.0273 | 0.0072 | 0.0037 | 0.0003 | 0.0027 | 0.0161 | 0.001 | 0.0035 | 0.0002 | 0.0001 | 0.0001 | 0.0002 | 0 | 0 |
Lipid Particles | 0.0296 | 0.0005 | 0.0042 | 0.0026 | 0.0431 | 0.0113 | 0.0538 | 0.0027 | 0.0067 | 0.0028 | 0.0013 | 0.0026 | 0.0046 | 0.0002 | 0.0001 | 0.0089 | 0 | 0.0001 |
Mitochondria | 0.0038 | 0.0002 | 0.001 | 0.0011 | 0.0472 | 0.002 | 0.001 | 0.0001 | 0.0025 | 0.0015 | 0.0002 | 0.0032 | 0.0003 | 0.0002 | 0.0001 | 0.0002 | 0 | 0 |
None | 0.6418 | 0.8789 | 0.8081 | 0.8131 | 0.5725 | 0.7922 | 0.7078 | 0.6352 | 0.6862 | 0.5641 | 0.5182 | 0.6327 | 0.9334 | 0.8947 | 0.868 | 0.6168 | 0.9752 | 0.7883 |
Nuclear Periphery | 0.0061 | 0.0002 | 0.0053 | 0.0009 | 0.0042 | 0.0038 | 0.0042 | 0.0004 | 0.0005 | 0.0009 | 0.0002 | 0.0005 | 0.0005 | 0.0003 | 0.0003 | 0.0003 | 0 | 0.0001 |
Nucleolus | 0.003 | 0.0002 | 0.0003 | 0.0016 | 0.0008 | 0.0007 | 0.0027 | 0.0001 | 0.0006 | 0.0003 | 0.0001 | 0.0003 | 0.0003 | 0.0004 | 0.0001 | 0.0004 | 0 | 0 |
Nucleus | 0.0111 | 0.0005 | 0.0073 | 0.0016 | 0.0028 | 0.0006 | 0.0026 | 0.0006 | 0.0008 | 0.0009 | 0.0004 | 0.0009 | 0.0004 | 0.0017 | 0.0004 | 0.0008 | 0 | 0.0002 |
Peroxisomes | 0.0162 | 0.0001 | 0.0289 | 0.0009 | 0.0069 | 0.0028 | 0.0024 | 0 | 0.0016 | 0.0005 | 0.0001 | 0.0042 | 0.0002 | 0 | 0 | 0.0002 | 0 | 0 |
Punctate Nuclear | 0.0203 | 0.0003 | 0.0008 | 0.0032 | 0.0034 | 0.0022 | 0.0154 | 0.0002 | 0.0005 | 0.0005 | 0.0002 | 0.0018 | 0.0006 | 0.0034 | 0.0001 | 0.0005 | 0 | 0 |
Vacuole | 0.0065 | 0.0027 | 0.009 | 0.0126 | 0.0179 | 0.0131 | 0.009 | 0.0063 | 0.0114 | 0.0026 | 0.007 | 0.0144 | 0.0012 | 0.0494 | 0.031 | 0.0051 | 0 | 0.0202 |
Vacuole Periphery | 0.0014 | 0.0001 | 0.0008 | 0.0022 | 0.0015 | 0.0019 | 0.0009 | 0.0001 | 0.0005 | 0.0008 | 0.0004 | 0.0003 | 0.0001 | 0.0056 | 0.0003 | 0.0001 | 0 | 0.002 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 21.6158 | 28.5389 | 19.8535 | 20.5851 | 17.2467 | 31.2425 | 42.9829 | 37.3283 | 36.4286 | 45.7914 |
Translational Efficiency | 0.6636 | 0.9801 | 0.8666 | 0.8275 | 0.6909 | 0.523 | 0.7963 | 0.8459 | 0.7405 | 0.7635 |
Dataset | Hit |
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Protein Concentration | ✔ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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