Standard name
Human Ortholog
Description Protein that facilitates ribosomal subunit dissociation; Dom34-Hbs1 complex and Rli1p have roles in dissociating inactive ribosomes to facilitate translation restart, particularly ribosomes stalled in 3' UTRs; required for RNA cleavage in no-go decay, but reports conflict on endonuclease activity; Pelota ortholog; protein abundance increases in response to DNA replication stress; DOM34 has a paralog, YCL001W-B, that arose from the whole genome duplication
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0.07 0 0 0 0.05 0.07 0.07 0.07 0.08 0.09 0 0.06 0 0.05 0.06 0.05 0 0 0 0 0 0
Cytoplasm 0.99 0.99 0.97 0.95 0.93 0.86 0.71 0.73 0.69 0.66 0.67 0.66 0.99 0.99 0.99 0.96 0.96 0.95 0.97 0.97 0.94 0.96 0.96 0.97
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0.08 0.08 0 0.05 0 0 0 0.05 0.05 0.05 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0.05 0.2 0.31 0.22 0.26 0.32 0.32 0.32 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0.06 0 0 0.06 0 0 0 0 0 0 0.05 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 1 0 2 0 4 6 11 4 9 12 9 0 0 0 0 0 0 0 0 0 0 1 0
Bud 0 0 4 1 3 3 12 7 11 9 19 18 0 0 1 0 0 0 0 0 1 0 0 1
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 2 3 22 11 6 18 31 45 34 37 32 46 9 28 14 6 9 7 0 0 1 0 0 0
Cytoplasm 174 129 314 278 334 375 439 505 317 327 280 327 322 444 543 124 137 122 166 140 309 237 386 350
Endoplasmic Reticulum 1 3 6 0 7 13 27 25 37 40 15 25 2 6 5 6 7 6 0 0 8 0 2 0
Endosome 0 0 0 0 0 0 1 5 0 1 0 1 0 0 0 0 0 0 0 0 0 0 1 1
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 1 0 1 0 1 1 0 0 1 0 0 0
Mitochondria 0 0 2 10 18 87 195 153 120 158 133 158 0 0 1 2 3 0 1 0 1 0 1 2
Nucleus 1 0 0 0 1 2 3 1 0 1 1 1 1 1 2 0 1 0 1 0 0 2 1 0
Nuclear Periphery 0 0 0 0 1 1 3 1 0 1 0 1 0 0 0 0 0 0 0 0 1 0 0 0
Nucleolus 0 0 0 0 0 0 1 2 0 0 0 1 0 0 0 0 1 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1
Vac/Vac Membrane 0 0 1 8 9 4 23 40 17 16 23 14 0 0 0 1 2 7 0 1 0 2 4 1
Unique Cell Count 175 130 324 292 361 436 621 691 460 497 418 493 324 449 546 129 142 129 172 145 330 247 401 362
Labelled Cell Count 178 136 349 310 379 507 741 795 540 599 515 601 335 479 567 139 161 143 172 145 330 247 401 362


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 12.0 17.0 14.0 12.2 10.9 9.4 8.9 9.2 8.7 8.4 8.2 8.2 15.5 16.0 15.4 17.7 17.4 19.7 14.4 14.8 15.8
Std Deviation (1e-4) 1.9 2.1 1.7 1.6 1.8 1.5 1.7 1.8 1.8 1.8 1.7 1.7 2.7 2.5 2.2 5.0 4.7 4.1 2.4 2.7 2.8
Intensity Change (Log2) – – – -0.2 -0.36 -0.58 -0.65 -0.61 -0.69 -0.73 -0.78 -0.77 0.15 0.2 0.14 0.34 0.31 0.49 0.04 0.08 0.17


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 3.1 0 0 0 0 0 0 0
Bud 0 0 0 0.8 0 1.2 0.6 2.6 2.1 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery -1.7 -3.4 -1.6 -1.1 -0.2 0.3 0.4 0.5 1.3 -2.4 -0.3 -3.0 -0.9 -0.2 -0.5
Cytoplasm -1.1 -2.5 -5.1 -9.5 -9.0 -9.7 -10.5 -10.1 -10.4 2.3 2.0 3.0 -0.4 -0.2 -1.2
Endoplasmic Reticulum 0 0.1 1.0 2.0 1.5 3.7 3.8 1.4 2.4 0 -0.6 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 2.5 3.4 8.2 11.1 8.9 9.7 11.0 10.9 11.1 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 3.1 4.1 3.1 2.9 4.0 2.6 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 11.0065 13.5474 11.9633 10.9782 11.3637 11.9796 9.3789 12.9115 12.1728 10.6985 11.038 11.7975 9.0515 11.9813 10.3185 10.4525 8.4739 10.8888
Actin 0.0002 0.0001 0.0007 0.0002 0.0001 0.0004 0.0058 0.0002 0.0101 0.0066 0.0007 0.0002 0.0348 0.0009 0.0031 0.0176 0.0297 0.0104
Bud 0.0001 0.0001 0.0013 0.0002 0.0001 0.0001 0.0007 0.0001 0.0003 0.0007 0.0007 0.0001 0.0005 0.0003 0.0004 0.0005 0.0004 0.0001
Bud Neck 0.0002 0.0001 0.0002 0.0004 0.0006 0.0006 0.0009 0.0001 0.0004 0.0003 0.0004 0.0008 0.0007 0.0001 0.0007 0.0004 0.0005 0.0004
Bud Periphery 0 0 0.0006 0 0 0.0001 0.0009 0 0.0002 0.0008 0.0015 0.0001 0.0006 0.0001 0.0004 0.0001 0.0005 0.0001
Bud Site 0.0002 0.0002 0.0017 0.0001 0 0 0.0008 0.0001 0.0005 0.0027 0.0006 0.0001 0.0016 0.001 0.001 0.0002 0.0012 0.0001
Cell Periphery 0 0 0.0001 0 0 0 0.0003 0 0 0.0012 0.0001 0 0.0002 0 0.0002 0 0.0001 0
Cytoplasm 0.9571 0.9886 0.9744 0.9409 0.9707 0.9758 0.8351 0.9941 0.9581 0.9267 0.8931 0.9611 0.8551 0.9806 0.9247 0.9471 0.7834 0.9563
Cytoplasmic Foci 0.0131 0.0021 0.0035 0.0072 0.0046 0.0042 0.0291 0.0013 0.0049 0.0062 0.0034 0.0077 0.0113 0.0044 0.0141 0.012 0.0442 0.0041
Eisosomes 0 0 0 0 0 0 0.0001 0 0 0.0001 0 0 0.0004 0 0.0001 0 0.0002 0.0001
Endoplasmic Reticulum 0.0069 0.0023 0.0027 0.0044 0.0157 0.0076 0.0092 0.0008 0.0013 0.002 0.003 0.0093 0.0068 0.001 0.0068 0.0033 0.0017 0.003
Endosome 0.0115 0.0021 0.0079 0.0356 0.0044 0.0067 0.0411 0.0012 0.0074 0.0105 0.0411 0.0097 0.0213 0.0031 0.0143 0.0075 0.0176 0.0074
Golgi 0.0007 0.0001 0.0019 0.001 0.0002 0.0007 0.0074 0.0001 0.0065 0.0068 0.0026 0.0005 0.0111 0.0025 0.0018 0.0022 0.0142 0.0012
Lipid Particles 0.0007 0 0 0 0 0 0.0076 0 0.0008 0.0192 0.0009 0.0001 0.0116 0.0001 0.0049 0 0.0224 0.0015
Mitochondria 0.0002 0.0001 0.0007 0.0004 0.0001 0.0008 0.0233 0.0002 0.0046 0.0037 0.0198 0.0056 0.0164 0.0032 0.0047 0.0004 0.0424 0.0006
None 0.0018 0.0008 0.0008 0.0006 0.0006 0.0006 0.0059 0.0004 0.0005 0.0006 0.0008 0.0006 0.0026 0.0005 0.0048 0.0005 0.0019 0.003
Nuclear Periphery 0.0008 0.0008 0.0005 0.0008 0.0006 0.0004 0.007 0.0002 0.0005 0.0011 0.0078 0.0007 0.0091 0.0002 0.0021 0.0008 0.0023 0.0046
Nucleolus 0.0006 0 0 0 0 0 0.0006 0 0 0.0001 0.0002 0 0.0003 0 0.0005 0 0.0002 0.0006
Nucleus 0.0012 0.0009 0.001 0.0015 0.0012 0.0008 0.0047 0.0006 0.0011 0.0021 0.0028 0.0014 0.0037 0.0006 0.0021 0.0013 0.0011 0.0024
Peroxisomes 0.0002 0 0 0 0 0 0.0066 0 0.0013 0.0051 0.0002 0.0003 0.006 0.0006 0.0081 0.0024 0.0269 0.0003
Punctate Nuclear 0.0027 0.0001 0.0004 0.0001 0.0001 0.0001 0.0043 0 0.0001 0.0004 0.0004 0.0002 0.0017 0 0.0013 0.0001 0.008 0.0023
Vacuole 0.0013 0.0012 0.0011 0.005 0.0008 0.0008 0.0059 0.0004 0.001 0.0023 0.0129 0.0012 0.0028 0.0005 0.0029 0.0027 0.0008 0.0008
Vacuole Periphery 0.0002 0.0002 0.0003 0.0014 0.0002 0.0003 0.0027 0.0001 0.0003 0.0006 0.007 0.0004 0.0014 0.0001 0.001 0.0008 0.0004 0.0005

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 26.611 33.9837 34.0434 36.4164 19.7156 18.0474 33.9313 35.6566 27.9943 34.5608
Translational Efficiency 2.0903 1.7324 1.5477 1.557 2.1996 2.4422 1.582 1.4535 1.6586 1.5777

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✔
Standard name
Human Ortholog
Description Protein that facilitates ribosomal subunit dissociation; Dom34-Hbs1 complex and Rli1p have roles in dissociating inactive ribosomes to facilitate translation restart, particularly ribosomes stalled in 3' UTRs; required for RNA cleavage in no-go decay, but reports conflict on endonuclease activity; Pelota ortholog; protein abundance increases in response to DNA replication stress; DOM34 has a paralog, YCL001W-B, that arose from the whole genome duplication
Localization
Cell Percentages cytoplasm (100%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-7

Dom34

Dom34


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Dom34-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available