Standard name | |
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Human Ortholog | |
Description | Poly(A+) RNA-binding protein; key surveillance factor for the selective export of spliced mRNAs from the nucleus to the cytoplasm; preference for intron-containing genes; similar to Npl3p; HRB1 has a paralog, GBP2, that arose from the whole genome duplication |
ensLOC | DeepLoc | |||||||||||||||||||||||
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Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 0 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 4 | 8 | 5 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 1 | 2 | 3 | 6 | 6 | 11 | 8 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 4 | 2 | 3 | 4 | 7 | 4 | 17 | 8 | 4 | 4 | 5 | 1 | 39 | 47 | 83 | 19 | 28 | 13 | 0 | 0 | 0 | 5 | 1 | 1 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 2 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 4 | 2 | 2 | 0 | 0 | 0 | 0 | 2 | 0 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 3 | 0 | 1 | 0 | 4 | 1 | 1 | 2 | 3 | 1 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 1 | 0 | 0 |
Mitochondria | 3 | 3 | 0 | 0 | 4 | 7 | 23 | 17 | 11 | 25 | 19 | 33 | 0 | 0 | 0 | 2 | 3 | 5 | 0 | 0 | 0 | 0 | 4 | 4 |
Nucleus | 222 | 168 | 211 | 122 | 144 | 181 | 261 | 267 | 238 | 289 | 182 | 271 | 349 | 420 | 386 | 129 | 210 | 110 | 219 | 165 | 216 | 280 | 465 | 335 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 1 | 3 | 3 | 1 | 3 | 0 | 0 | 5 | 0 | 1 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 3 |
Nucleolus | 14 | 5 | 11 | 8 | 27 | 59 | 91 | 106 | 120 | 149 | 87 | 135 | 7 | 17 | 20 | 7 | 12 | 6 | 3 | 2 | 6 | 8 | 40 | 71 |
Peroxisomes | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 4 | 6 | 13 | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 2 | 4 |
Vac/Vac Membrane | 0 | 2 | 1 | 2 | 0 | 1 | 2 | 2 | 3 | 2 | 2 | 0 | 4 | 5 | 12 | 3 | 13 | 4 | 4 | 3 | 3 | 24 | 90 | 121 |
Unique Cell Count | 233 | 174 | 219 | 130 | 161 | 214 | 323 | 329 | 297 | 367 | 234 | 352 | 396 | 486 | 498 | 160 | 259 | 133 | 239 | 179 | 230 | 334 | 623 | 553 |
Labelled Cell Count | 244 | 180 | 226 | 136 | 186 | 261 | 400 | 407 | 385 | 481 | 304 | 449 | 404 | 499 | 524 | 165 | 270 | 140 | 239 | 179 | 230 | 334 | 623 | 553 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 13.3 | 16.3 | 15.5 | 12.2 | 11.0 | 9.2 | 8.9 | 8.9 | 8.4 | 8.2 | 7.6 | 7.6 | 16.9 | 16.1 | 15.6 | 18.4 | 17.8 | 18.0 | 14.6 | 16.2 | 17.3 |
Std Deviation (1e-4) | 4.0 | 4.8 | 4.7 | 2.8 | 2.7 | 2.6 | 3.2 | 3.4 | 2.4 | 2.8 | 2.4 | 2.2 | 6.7 | 6.5 | 8.1 | 7.6 | 6.6 | 7.3 | 4.5 | 5.3 | 5.5 |
Intensity Change (Log2) | – | – | – | -0.35 | -0.49 | -0.75 | -0.8 | -0.8 | -0.89 | -0.91 | -1.02 | -1.02 | 0.13 | 0.05 | 0.01 | 0.25 | 0.2 | 0.22 | -0.08 | 0.07 | 0.16 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 0 | 0 | 0 | 2.4 | 0 | 0 | 0 | 0 | 0 | 4.0 | 4.0 | 5.8 | 4.3 | 4.2 | 3.7 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 0 | 4.0 | 3.4 | 0 | 3.9 | 4.3 | 4.7 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | -1.1 | -2.7 | -4.2 | -5.3 | -5.2 | -5.4 | -5.8 | -5.8 | -6.2 | -3.4 | -4.0 | -6.2 | -5.0 | -5.1 | -4.4 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0.5 | 3.8 | 6.4 | 6.8 | 7.6 | 9.1 | 9.4 | 8.3 | 8.9 | -2.3 | -1.0 | -0.6 | -0.3 | -0.2 | -0.2 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2.9 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 8.9891 | 11.1688 | 10.783 | 9.848 | 10.3752 | 10.4236 | 10.8418 | 11.9962 | 10.8259 | 10.8156 | 9.4852 | 10.5 | 11.6862 | 12.493 | 11.4138 | 10.0635 | 10.7242 | 11.0251 |
Actin | 0 | 0 | 0 | 0 | 0 | 0 | 0.0169 | 0.0033 | 0.0115 | 0.0035 | 0.0046 | 0.0032 | 0.0088 | 0 | 0.0179 | 0 | 0.005 | 0.0003 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0.0014 | 0 | 0.0003 | 0.0001 | 0.0013 | 0.0001 | 0.0003 | 0 | 0.0001 | 0 | 0.0008 | 0 |
Bud Neck | 0.0001 | 0 | 0 | 0 | 0.0001 | 0.0002 | 0.001 | 0 | 0.0002 | 0.0001 | 0.0003 | 0.0007 | 0.0004 | 0 | 0.0001 | 0 | 0.0006 | 0.0002 |
Bud Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0.0028 | 0 | 0.0003 | 0.0001 | 0.0015 | 0.0001 | 0.0006 | 0 | 0.0001 | 0 | 0.0013 | 0.0001 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0.0042 | 0.0001 | 0.0012 | 0.0002 | 0.0099 | 0.0001 | 0.0004 | 0 | 0.0002 | 0 | 0.0019 | 0.0001 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0.0007 | 0 | 0.0001 | 0 | 0.0005 | 0.0001 | 0.0004 | 0 | 0.0001 | 0 | 0.0006 | 0.0001 |
Cytoplasm | 0.0009 | 0 | 0.0001 | 0 | 0 | 0 | 0.008 | 0.0003 | 0.0001 | 0.0001 | 0.006 | 0.0001 | 0.0025 | 0 | 0.001 | 0 | 0.0046 | 0.0011 |
Cytoplasmic Foci | 0.0001 | 0 | 0 | 0 | 0 | 0 | 0.0176 | 0.0007 | 0.0012 | 0.0049 | 0.0081 | 0.0007 | 0.0032 | 0 | 0.0019 | 0 | 0.0089 | 0.0056 |
Eisosomes | 0 | 0 | 0 | 0 | 0 | 0 | 0.0004 | 0 | 0 | 0 | 0.0001 | 0 | 0.0002 | 0 | 0.0001 | 0 | 0.0001 | 0 |
Endoplasmic Reticulum | 0.0001 | 0 | 0 | 0 | 0 | 0 | 0.0016 | 0.0001 | 0.0001 | 0 | 0.0018 | 0.0005 | 0.0036 | 0 | 0.0005 | 0 | 0.0021 | 0.0001 |
Endosome | 0.0005 | 0 | 0.0001 | 0 | 0 | 0 | 0.01 | 0.0009 | 0.0018 | 0.004 | 0.0235 | 0.0032 | 0.0078 | 0 | 0.0021 | 0 | 0.0077 | 0.0008 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0.0063 | 0.0009 | 0.0046 | 0.009 | 0.006 | 0.0025 | 0.0023 | 0 | 0.0021 | 0 | 0.0014 | 0.0003 |
Lipid Particles | 0.0001 | 0 | 0 | 0 | 0 | 0 | 0.0515 | 0.0006 | 0.0021 | 0.008 | 0.0031 | 0.0031 | 0.0039 | 0 | 0.0018 | 0 | 0.008 | 0.0026 |
Mitochondria | 0.0005 | 0.0001 | 0.0006 | 0.0001 | 0.0006 | 0.0005 | 0.0098 | 0.0004 | 0.0044 | 0.0042 | 0.0269 | 0.003 | 0.0051 | 0.0001 | 0.0008 | 0 | 0.0058 | 0.0002 |
None | 0.004 | 0 | 0.0001 | 0 | 0 | 0 | 0.0025 | 0 | 0 | 0 | 0.0016 | 0.0002 | 0.0014 | 0 | 0.0007 | 0 | 0.0029 | 0.0004 |
Nuclear Periphery | 0.005 | 0.0003 | 0.0024 | 0.0002 | 0.0011 | 0.0001 | 0.0073 | 0.0011 | 0.0004 | 0.0009 | 0.0043 | 0.0028 | 0.0182 | 0.0009 | 0.0032 | 0.0002 | 0.0254 | 0.0008 |
Nucleolus | 0.0369 | 0.0091 | 0.0111 | 0.026 | 0.1276 | 0.06 | 0.0265 | 0.0094 | 0.0079 | 0.0067 | 0.0887 | 0.0674 | 0.0605 | 0.0094 | 0.0074 | 0.0085 | 0.1177 | 0.0546 |
Nucleus | 0.9472 | 0.9904 | 0.9845 | 0.9736 | 0.8688 | 0.9387 | 0.7948 | 0.9813 | 0.9599 | 0.9422 | 0.7976 | 0.8994 | 0.8648 | 0.9893 | 0.9481 | 0.9912 | 0.7849 | 0.9244 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0.0233 | 0.0006 | 0.0009 | 0.0155 | 0.0017 | 0.0003 | 0.0008 | 0 | 0.0086 | 0 | 0.001 | 0.0002 |
Punctate Nuclear | 0.004 | 0 | 0.0001 | 0 | 0.0014 | 0 | 0.0065 | 0.0001 | 0.0001 | 0.0002 | 0.0016 | 0.0118 | 0.0108 | 0 | 0.0026 | 0 | 0.0119 | 0.0077 |
Vacuole | 0.0004 | 0 | 0.0004 | 0 | 0.0001 | 0.0002 | 0.0042 | 0.0001 | 0.0015 | 0.0001 | 0.0039 | 0.0003 | 0.0019 | 0.0001 | 0.0002 | 0 | 0.0042 | 0.0002 |
Vacuole Periphery | 0.0002 | 0 | 0.0006 | 0 | 0.0002 | 0.0001 | 0.0027 | 0.0002 | 0.0015 | 0.0001 | 0.0068 | 0.0004 | 0.0022 | 0.0001 | 0.0002 | 0 | 0.0031 | 0.0002 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 22.6339 | 29.6274 | 24.6659 | 29.7757 | 28.2978 | 22.6214 | 29.7736 | 28.1086 | 32.1764 | 29.7395 |
Translational Efficiency | 2.1118 | 1.6529 | 1.8448 | 1.5855 | 1.6338 | 1.9901 | 1.7165 | 1.686 | 1.5459 | 1.8199 |
Dataset | Hit |
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Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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