Standard name
Human Ortholog
Description Poly (A)+ RNA-binding protein; abundant mRNP-component protein that binds mRNA and is required for stability of many mRNAs; component of glucose deprivation induced stress granules, involved in P-body-dependent granule assembly; implicated in regulation of translation; carries Q/N-rich domain at C- terminus, identified as candidate prion; human homolog Tia1 is critical for normal synaptic plasticity; protein abundance increases in response to DNA replication stress
DOWNLOAD

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0.05 0 0.06 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0.05 0 0 0.05 0.06 0.06 0.07 0.05 0.12 0.09 0 0.05 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.99 0.98 0.97 0.96 0.96 0.89 0.8 0.75 0.67 0.72 0.66 0.69 0.99 1.0 1.0 0.98 0.98 0.98 0.95 0.93 0.94 0.97 0.96 0.94
Endoplasmic Reticulum 0 0 0 0 0 0.07 0.13 0.19 0.23 0.18 0.19 0.22 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0.05 0.05 0.06 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 1 3 8 12 26 17 24 16 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 4 0 0 0 2 3 7 1 4 8 3 0 0 0 0 0 0 0 3 2 2 2 7
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 2 23 9 4 19 26 34 35 30 45 43 9 20 14 9 11 7 0 0 1 0 0 0
Cytoplasm 162 359 455 254 306 371 359 431 339 409 250 319 401 419 453 400 447 436 155 330 439 383 521 473
Endoplasmic Reticulum 0 6 12 5 12 29 60 108 114 104 71 100 5 2 2 16 14 15 0 3 19 2 2 6
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 1 0 0 0 0 0 0
Golgi 0 0 1 2 0 0 2 7 7 2 5 1 0 0 0 2 2 1 0 0 0 0 0 0
Mitochondria 2 0 0 0 1 9 18 15 27 30 24 5 0 0 0 1 0 0 1 0 1 0 1 0
Nucleus 0 0 0 0 1 0 1 0 1 1 0 0 0 0 0 1 1 2 0 4 0 0 2 2
Nuclear Periphery 0 0 0 1 0 0 0 0 0 0 1 2 0 0 0 0 0 0 0 1 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 2 1 1 0 0 0 0 0 1 1 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 1
Vac/Vac Membrane 0 3 2 0 1 0 0 11 12 4 10 22 1 1 2 4 3 5 0 7 2 1 6 3
Unique Cell Count 164 368 468 265 319 418 451 576 503 565 381 462 404 421 454 410 456 443 164 356 469 394 540 501
Labelled Cell Count 164 374 493 271 326 433 477 625 562 603 439 512 416 442 471 434 478 468 164 356 469 394 540 501


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 49.1 49.0 47.9 42.8 40.9 37.5 34.6 33.9 33.2 31.2 32.9 31.7 56.8 53.6 52.4 67.9 59.7 67.3 56.2 57.6 64.0
Std Deviation (1e-4) 11.6 12.4 9.6 9.1 8.0 7.1 7.8 8.2 7.7 7.6 9.4 7.9 12.6 12.0 10.4 23.7 18.2 23.2 13.8 14.1 16.2
Intensity Change (Log2) – – – -0.16 -0.23 -0.35 -0.47 -0.5 -0.53 -0.62 -0.54 -0.6 0.24 0.16 0.13 0.5 0.32 0.49 0.23 0.26 0.42


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 3.1 5.0 3.8 5.5 4.1 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery -1.0 -2.8 -0.3 0.6 0.7 1.3 0.3 3.7 2.6 -2.1 -0.1 -1.4 -2.1 -2.0 -2.8
Cytoplasm -1.0 -1.0 -5.0 -8.4 -10.0 -12.0 -10.7 -12.2 -11.5 2.2 2.7 3.2 0.3 0.8 1.2
Endoplasmic Reticulum -0.6 1.0 3.1 6.1 8.2 9.3 8.0 7.8 8.9 -1.4 -2.5 -2.6 1.1 0.5 0.7
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 4.4 3.5 5.1 5.1 5.5 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 2.1 2.6 0 2.7 4.2 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 50.6991 58.9833 56.699 54.0101 51.3832 55.8568 51.2429 67.6896 62.0342 61.0028 54.4925 60.5796 57.851 67.4197 62.2075 55.2144 54.5113 61.297
Actin 0.0026 0.0009 0.0002 0.0003 0.0001 0.0017 0.0118 0.0039 0.0043 0.0002 0.0366 0.0059 0.0147 0.0004 0.0005 0.0422 0.0001 0.0003
Bud 0.0004 0.0001 0.0003 0.0004 0.0001 0.0007 0.0017 0.0001 0.0005 0.0001 0.0006 0.0005 0.0004 0.0001 0.0002 0.0005 0.0002 0.0002
Bud Neck 0.0004 0.0002 0.0003 0.0003 0.0003 0.0009 0.0009 0.0001 0.0005 0.0003 0.0004 0.0012 0.0012 0.0002 0.0003 0.0035 0.0005 0.0013
Bud Periphery 0.0003 0 0.0001 0.0002 0.0001 0.0003 0.0015 0.0001 0.0006 0 0.0005 0.0004 0.0004 0.0001 0.0003 0.0005 0.0002 0.0003
Bud Site 0.0003 0.0008 0.0001 0.0001 0 0.0003 0.0019 0.0008 0.0053 0 0.0006 0.0001 0.0005 0.0001 0.0001 0.0007 0.0001 0.0001
Cell Periphery 0.0003 0.0002 0.0003 0.0002 0.0001 0.0001 0.0011 0.0002 0.001 0.0001 0.0004 0.0007 0.0014 0.0004 0.0015 0.0003 0.0008 0.0004
Cytoplasm 0.8669 0.9924 0.9432 0.9275 0.9542 0.9272 0.8391 0.9854 0.9375 0.9646 0.8696 0.9221 0.8476 0.9822 0.9448 0.8764 0.9145 0.9247
Cytoplasmic Foci 0.0117 0.0007 0.0045 0.0011 0.0032 0.0068 0.0147 0.0004 0.0044 0.0007 0.0078 0.0016 0.0033 0.0008 0.0045 0.0064 0.0041 0.0017
Eisosomes 0.0001 0 0 0 0 0 0.0003 0 0.0001 0 0.0002 0.0001 0.0002 0 0 0.0008 0 0
Endoplasmic Reticulum 0.0149 0.0009 0.0181 0.0086 0.0115 0.0245 0.0262 0.0054 0.0044 0.0144 0.0056 0.02 0.0564 0.0084 0.0175 0.0023 0.0081 0.0343
Endosome 0.0141 0.0003 0.0047 0.0062 0.0114 0.0077 0.014 0.0002 0.0034 0.0013 0.0154 0.0049 0.0093 0.0008 0.003 0.0047 0.0172 0.0044
Golgi 0.0013 0.0001 0.0002 0.0002 0.0003 0.0011 0.0026 0.0001 0.0012 0 0.0185 0.0012 0.0018 0.0002 0.0005 0.0034 0.0011 0.0002
Lipid Particles 0.0047 0.0001 0.0003 0 0.0002 0.0006 0.0082 0 0.0032 0.0001 0.0116 0.0008 0.0025 0.0001 0.005 0.0154 0.0007 0.0001
Mitochondria 0.0014 0 0.0006 0.0002 0.0003 0.0013 0.0079 0.0001 0.0132 0.0001 0.0068 0.0017 0.0022 0.0008 0.0036 0.0201 0.0074 0.0007
None 0.0071 0.0001 0.0009 0.0002 0.0013 0.0011 0.0056 0.0001 0.0019 0.0001 0.0035 0.0004 0.0024 0.0004 0.0014 0.0022 0.0021 0.0002
Nuclear Periphery 0.0198 0.0007 0.0064 0.0086 0.0031 0.0078 0.0167 0.0011 0.0032 0.008 0.0054 0.0177 0.0172 0.001 0.0056 0.005 0.0034 0.0066
Nucleolus 0.0005 0 0.0006 0 0 0.0014 0.0032 0 0.0009 0 0.0004 0.0003 0.0009 0 0.0012 0.0026 0.0003 0.0001
Nucleus 0.0106 0.0009 0.0028 0.0035 0.0017 0.0037 0.0134 0.0007 0.0026 0.003 0.0027 0.0042 0.0068 0.0011 0.0021 0.0025 0.0029 0.0024
Peroxisomes 0.0014 0 0.0001 0 0 0.0001 0.001 0.0002 0.0006 0 0.0021 0.0002 0.0047 0 0.0007 0.0013 0 0
Punctate Nuclear 0.006 0 0.0001 0.0001 0.0001 0.0017 0.007 0 0.0002 0.0001 0.0019 0.0012 0.0011 0 0.0002 0.0018 0.0001 0
Vacuole 0.0269 0.0012 0.0129 0.031 0.0095 0.008 0.0165 0.0011 0.0082 0.006 0.0071 0.0104 0.0187 0.0025 0.0055 0.0039 0.0234 0.0114
Vacuole Periphery 0.0083 0.0001 0.0031 0.0112 0.0023 0.003 0.0046 0.0001 0.0025 0.0008 0.002 0.0045 0.0063 0.0003 0.0015 0.0034 0.0129 0.0105

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 233.319 220.8845 161.2189 234.4357 215.8523 172.4633 216.0136 206.5836 187.6793 232.5825
Translational Efficiency 2.1949 2.1937 2.5844 1.408 2.1793 3.2271 2.0573 1.908 2.1285 2.0634

Hit Data

Dataset Hit
Protein Concentration ✔
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Poly (A)+ RNA-binding protein; abundant mRNP-component protein that binds mRNA and is required for stability of many mRNAs; component of glucose deprivation induced stress granules, involved in P-body-dependent granule assembly; implicated in regulation of translation; carries Q/N-rich domain at C- terminus, identified as candidate prion; human homolog Tia1 is critical for normal synaptic plasticity; protein abundance increases in response to DNA replication stress
Localization
Cell Percentages cytoplasm (100%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-2

Pub1

Pub1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Pub1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available