Standard name | |
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Human Ortholog | |
Description | Ser/Thr protein kinase; phosphorylates Pan1p-Sla1p-End3p protein complex subunits, Pan1p and Sla1p; involved in regulation of the cortical actin cytoskeleton and endocytosis; functional overlap with PRK1 |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 40 | 111 | 18 | 30 | 24 | 27 | 17 | – | 12 | 13 | 7 | 12 | 21 | 31 | 12 | 77 | 57 | 38 | 86 | 244 | 49 | 41 | 115 | 108 |
Bud | 25 | 30 | 7 | 8 | 17 | 17 | 23 | – | 22 | 20 | 23 | 9 | 2 | 13 | 8 | 27 | 31 | 15 | 11 | 19 | 3 | 12 | 33 | 24 |
Bud Neck | 0 | 0 | 1 | 1 | 1 | 1 | 1 | – | 0 | 0 | 1 | 0 | 0 | 0 | 3 | 0 | 6 | 0 | 5 | 17 | 6 | 14 | 21 | 18 |
Bud Site | 6 | 3 | 0 | 2 | 0 | 2 | 7 | – | 1 | 1 | 6 | 4 | 0 | 1 | 0 | 1 | 2 | 3 | – | – | – | – | – | – |
Cell Periphery | 6 | 12 | 3 | 4 | 4 | 2 | 6 | – | 1 | 1 | 1 | 1 | 6 | 5 | 6 | 21 | 14 | 17 | 0 | 3 | 1 | 2 | 4 | 2 |
Cytoplasm | 56 | 149 | 44 | 47 | 80 | 47 | 70 | – | 33 | 56 | 32 | 31 | 134 | 160 | 179 | 93 | 81 | 83 | 21 | 47 | 17 | 29 | 40 | 40 |
Endoplasmic Reticulum | 10 | 39 | 2 | 3 | 6 | 12 | 4 | – | 5 | 5 | 6 | 10 | 8 | 8 | 6 | 9 | 15 | 13 | 0 | 2 | 4 | 0 | 2 | 2 |
Endosome | 0 | 5 | 7 | 0 | 15 | 9 | 19 | – | 8 | 12 | 3 | 3 | 1 | 11 | 10 | 18 | 26 | 29 | 10 | 26 | 8 | 4 | 8 | 9 |
Golgi | 0 | 2 | 0 | 0 | 1 | 0 | 0 | – | 0 | 0 | 0 | 2 | 0 | 1 | 0 | 10 | 3 | 3 | 0 | 3 | 0 | 0 | 1 | 1 |
Mitochondria | 40 | 15 | 3 | 9 | 7 | 41 | 49 | – | 95 | 77 | 138 | 80 | 0 | 1 | 1 | 23 | 9 | 8 | 14 | 41 | 27 | 21 | 21 | 35 |
Nucleus | 1 | 0 | 2 | 0 | 0 | 1 | 0 | – | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 1 | 4 | 2 | 0 | 1 | 0 | 1 | 2 | 1 |
Nuclear Periphery | 1 | 1 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 4 | 6 | 2 | 3 | 25 | 24 | 25 | – | 11 | 15 | 0 | 2 | 0 | 5 | 0 | 12 | 20 | 19 | 4 | 5 | 7 | 0 | 2 | 4 |
SpindlePole | 3 | 11 | 7 | 10 | 14 | 4 | 30 | – | 7 | 9 | 3 | 8 | 6 | 9 | 5 | 40 | 48 | 36 | 3 | 8 | 0 | 4 | 3 | 6 |
Vac/Vac Membrane | 0 | 3 | 1 | 1 | 3 | 1 | 11 | – | 2 | 2 | 1 | 1 | 0 | 1 | 1 | 10 | 13 | 20 | 0 | 6 | 3 | 3 | 4 | 2 |
Unique Cell Count | 137 | 324 | 82 | 92 | 160 | 137 | 202 | 148 | 167 | 173 | 130 | 164 | 218 | 213 | 255 | 229 | 210 | 162 | 433 | 134 | 147 | 267 | 264 | |
Labelled Cell Count | 192 | 387 | 97 | 118 | 197 | 188 | 262 | 198 | 211 | 222 | 164 | 178 | 247 | 231 | 343 | 329 | 287 | 162 | 433 | 134 | 147 | 267 | 264 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 4.7 | 6.0 | 6.5 | 6.0 | 6.6 | 5.6 | 5.8 | – | 4.9 | 5.3 | 4.4 | 5.1 | 6.1 | 6.3 | 6.2 | 8.0 | 8.4 | 7.9 | 5.7 | 6.2 | 6.5 |
Std Deviation (1e-4) | 0.5 | 0.8 | 1.3 | 1.9 | 1.6 | 1.7 | 1.6 | – | 1.5 | 1.2 | 1.4 | 1.4 | 0.8 | 1.0 | 0.9 | 1.6 | 2.3 | 1.9 | 1.3 | 1.4 | 1.2 |
Intensity Change (Log2) | – | – | – | -0.11 | 0.02 | -0.23 | -0.16 | – | -0.41 | -0.29 | -0.55 | -0.36 | -0.09 | -0.06 | -0.06 | 0.29 | 0.37 | 0.28 | -0.21 | -0.08 | -0.0 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 1.6 | -1.4 | -0.4 | -3.1 | – | -3.0 | -3.2 | 0 | -2.6 | -1.9 | -1.6 | -4.2 | 1.4 | 0.5 | -0.8 |
Bud | 0 | 0.5 | 0.9 | 0.7 | – | 1.4 | 0.8 | 0 | -0.4 | 0 | -0.8 | 0 | 0.5 | 1.2 | -0.4 |
Bud Neck | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1.4 | 0 | 1.3 |
Cytoplasm | -0.3 | -0.5 | -2.8 | -3.0 | – | -4.8 | -3.0 | -5.7 | -4.4 | 4.6 | 3.3 | 5.4 | -2.8 | -2.9 | -2.2 |
Endoplasmic Reticulum | 0 | 0 | 1.9 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 0.2 | -0.5 | 0.2 | – | -0.9 | -0.4 | 0 | 0 | 0 | 0 | 0 | -0.4 | 0.7 | 1.2 |
Golgi | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 1.6 | 0 | 4.7 | 4.1 | – | 8.9 | 6.7 | 0 | 8.4 | 0 | 0 | 0 | 1.6 | 0 | 0 |
Nucleus | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 3.1 | 3.3 | 2.6 | – | 0 | 1.9 | 0 | 0 | 0 | 0 | 0 | 0 | 1.9 | 2.0 |
SpindlePole | 0.5 | 0.1 | 0 | 1.4 | – | 0 | -1.0 | 0 | -0.7 | 0 | 0 | 0 | 1.6 | 2.5 | 1.9 |
Vacuole | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2.5 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | -0.5608 | -0.3061 | -0.3712 | -0.8371 | -0.3401 | -0.5458 | 0.7214 | 0.6907 | 0.8198 | 0.1388 | 0.7976 | 0.7122 | -0.2542 | -0.5607 | -0.3309 | -0.4526 | -0.3954 | -0.3316 |
Actin | 0.233 | 0.1718 | 0.3648 | 0.2453 | 0.2446 | 0.2756 | 0.2377 | 0.196 | 0.295 | 0.3948 | 0.2923 | 0.246 | 0.2399 | 0.1676 | 0.2862 | 0.2268 | 0.1937 | 0.2173 |
Bud | 0.004 | 0.0053 | 0.0285 | 0.0156 | 0.0032 | 0.0038 | 0.0081 | 0.0116 | 0.0246 | 0.0103 | 0.0183 | 0.0018 | 0.0048 | 0.007 | 0.0232 | 0.0138 | 0.0541 | 0.0023 |
Bud Neck | 0.0119 | 0.0146 | 0.0079 | 0.0509 | 0.0038 | 0.1911 | 0.0271 | 0.018 | 0.0136 | 0.003 | 0.0103 | 0.2073 | 0.0233 | 0.0095 | 0.0079 | 0.0006 | 0.0022 | 0.1533 |
Bud Periphery | 0.0235 | 0.0136 | 0.087 | 0.051 | 0.018 | 0.016 | 0.0175 | 0.027 | 0.0874 | 0.047 | 0.0472 | 0.0055 | 0.005 | 0.0075 | 0.0735 | 0.0818 | 0.0714 | 0.0067 |
Bud Site | 0.0377 | 0.1836 | 0.0215 | 0.006 | 0.0034 | 0.0053 | 0.082 | 0.2107 | 0.052 | 0.0031 | 0.006 | 0.0051 | 0.0408 | 0.1259 | 0.0273 | 0.0019 | 0.0057 | 0.0032 |
Cell Periphery | 0.0025 | 0.0032 | 0.002 | 0.0018 | 0.001 | 0.0015 | 0.0039 | 0.0032 | 0.0017 | 0.0009 | 0.0012 | 0.0011 | 0.0023 | 0.0022 | 0.0015 | 0.0005 | 0.0011 | 0.0013 |
Cytoplasm | 0.0849 | 0.1973 | 0.112 | 0.149 | 0.039 | 0.1254 | 0.0951 | 0.1374 | 0.0828 | 0.0764 | 0.0986 | 0.087 | 0.1394 | 0.2352 | 0.1403 | 0.192 | 0.1165 | 0.1918 |
Cytoplasmic Foci | 0.1243 | 0.1038 | 0.0569 | 0.0845 | 0.0466 | 0.1183 | 0.154 | 0.0527 | 0.0517 | 0.0364 | 0.1069 | 0.0837 | 0.125 | 0.0848 | 0.0815 | 0.1 | 0.1782 | 0.1383 |
Eisosomes | 0.0052 | 0.0013 | 0.0024 | 0.0026 | 0.0021 | 0.002 | 0.0026 | 0.0014 | 0.0015 | 0.0006 | 0.0026 | 0.0014 | 0.0021 | 0.0012 | 0.0025 | 0.0006 | 0.0013 | 0.0011 |
Endoplasmic Reticulum | 0.0058 | 0.0024 | 0.0024 | 0.0096 | 0.0053 | 0.0023 | 0.0033 | 0.0015 | 0.0053 | 0.0013 | 0.0038 | 0.0017 | 0.0054 | 0.0025 | 0.0036 | 0.0016 | 0.0038 | 0.0033 |
Endosome | 0.0138 | 0.0151 | 0.0118 | 0.0544 | 0.0445 | 0.0224 | 0.0344 | 0.004 | 0.018 | 0.0414 | 0.0358 | 0.0104 | 0.0181 | 0.0079 | 0.0191 | 0.0288 | 0.0055 | 0.0126 |
Golgi | 0.0289 | 0.0048 | 0.0104 | 0.0311 | 0.0232 | 0.0152 | 0.0207 | 0.0059 | 0.0151 | 0.0237 | 0.0185 | 0.0132 | 0.0168 | 0.0088 | 0.0136 | 0.0172 | 0.0083 | 0.0135 |
Lipid Particles | 0.0258 | 0.0071 | 0.0069 | 0.0036 | 0.0972 | 0.0078 | 0.0232 | 0.0021 | 0.0066 | 0.0171 | 0.0282 | 0.0047 | 0.0118 | 0.0016 | 0.009 | 0.0363 | 0.0042 | 0.0022 |
Mitochondria | 0.0322 | 0.0016 | 0.0287 | 0.0342 | 0.0984 | 0.0245 | 0.023 | 0.0092 | 0.0277 | 0.0526 | 0.0229 | 0.0135 | 0.0101 | 0.002 | 0.0069 | 0.0078 | 0.0084 | 0.0045 |
None | 0.2965 | 0.223 | 0.2097 | 0.2148 | 0.2662 | 0.136 | 0.1641 | 0.2668 | 0.222 | 0.1874 | 0.2032 | 0.2167 | 0.2814 | 0.2851 | 0.2399 | 0.2301 | 0.268 | 0.1958 |
Nuclear Periphery | 0.0026 | 0.0012 | 0.0008 | 0.0043 | 0.0072 | 0.001 | 0.0046 | 0.0009 | 0.0019 | 0.0016 | 0.0017 | 0.002 | 0.0058 | 0.0005 | 0.0058 | 0.0012 | 0.0005 | 0.0012 |
Nucleolus | 0.002 | 0.0015 | 0.0013 | 0.0004 | 0.0481 | 0.0005 | 0.0022 | 0.0006 | 0.0037 | 0.0042 | 0.0009 | 0.001 | 0.0014 | 0.0012 | 0.0017 | 0.0002 | 0.0003 | 0.0021 |
Nucleus | 0.0021 | 0.0097 | 0.0011 | 0.0016 | 0.004 | 0.0007 | 0.0039 | 0.0014 | 0.0129 | 0.0276 | 0.0013 | 0.0072 | 0.0022 | 0.0016 | 0.0042 | 0.0005 | 0.0009 | 0.0028 |
Peroxisomes | 0.0515 | 0.022 | 0.0347 | 0.0235 | 0.0286 | 0.0465 | 0.0826 | 0.0471 | 0.0672 | 0.0623 | 0.0834 | 0.084 | 0.0527 | 0.0343 | 0.0403 | 0.0538 | 0.0738 | 0.0373 |
Punctate Nuclear | 0.0087 | 0.015 | 0.004 | 0.0012 | 0.006 | 0.0022 | 0.005 | 0.0013 | 0.0043 | 0.0023 | 0.0109 | 0.005 | 0.0095 | 0.0105 | 0.0097 | 0.0022 | 0.001 | 0.0083 |
Vacuole | 0.0022 | 0.0019 | 0.0041 | 0.0096 | 0.0059 | 0.0015 | 0.004 | 0.0008 | 0.0039 | 0.004 | 0.0045 | 0.0012 | 0.0015 | 0.0029 | 0.0017 | 0.0015 | 0.0007 | 0.0008 |
Vacuole Periphery | 0.0007 | 0.0002 | 0.0011 | 0.0051 | 0.0036 | 0.0006 | 0.0011 | 0.0002 | 0.001 | 0.0021 | 0.0017 | 0.0005 | 0.0008 | 0.0004 | 0.0008 | 0.0008 | 0.0002 | 0.0003 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 5.1803 | 7.2036 | 4.5817 | 7.3948 | 3.7314 | 6.328 | 9.4946 | 11.763 | 7.8815 | 9.7312 |
Translational Efficiency | 1.4251 | 0.9885 | 1.2783 | 0.5665 | 1.3653 | 1.4966 | 0.9646 | 0.5509 | 0.9095 | 0.6704 |
Dataset | Hit |
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Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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