Standard name
Human Ortholog
Description Ser/Thr protein kinase; phosphorylates Pan1p-Sla1p-End3p protein complex subunits, Pan1p and Sla1p; involved in regulation of the cortical actin cytoskeleton and endocytosis; functional overlap with PRK1

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0.29 0.34 0.22 0.33 0.15 0.2 0.08 0.08 0.08 0 0.09 0.13 0.14 0.06 0.3 0.25 0.18 0.54 0.56 0.37 0.28 0.43 0.41
Bud 0.18 0.09 0.09 0.09 0.11 0.12 0.11 0.15 0.12 0.13 0.07 0 0.06 0 0.11 0.14 0.07 0.07 0 0 0.09 0.12 0.09
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.1 0.08 0.07
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.08 0.06 0.08 0 0 0 0 0 0
Cytoplasm 0.41 0.46 0.54 0.51 0.5 0.34 0.35 0.22 0.34 0.18 0.24 0.82 0.73 0.84 0.36 0.35 0.4 0.13 0.11 0.13 0.2 0.15 0.15
Endoplasmic Reticulum 0.07 0.12 0 0 0 0.09 0 0 0 0 0.08 0.05 0 0 0 0.07 0.06 0 0 0 0 0 0
Endosome 0 0 0.09 0 0.09 0.07 0.09 0.05 0.07 0 0 0 0.05 0.05 0.07 0.11 0.14 0.07 0.06 0.06 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.29 0.05 0 0.1 0 0.3 0.24 0.64 0.46 0.8 0.62 0 0 0 0.09 0 0 0.09 0.1 0.2 0.15 0.08 0.13
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0.16 0.18 0.12 0.07 0.09 0 0 0 0 0 0.05 0.09 0.09 0 0 0.05 0 0 0
SpindlePole 0 0 0.09 0.11 0.09 0 0.15 0.05 0.05 0 0.06 0 0 0 0.16 0.21 0.17 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0.06 0.1 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 40 111 18 30 24 27 17 12 13 7 12 21 31 12 77 57 38 86 244 49 41 115 108
Bud 25 30 7 8 17 17 23 22 20 23 9 2 13 8 27 31 15 11 19 3 12 33 24
Bud Neck 0 0 1 1 1 1 1 0 0 1 0 0 0 3 0 6 0 5 17 6 14 21 18
Bud Site 6 3 0 2 0 2 7 1 1 6 4 0 1 0 1 2 3
Cell Periphery 6 12 3 4 4 2 6 1 1 1 1 6 5 6 21 14 17 0 3 1 2 4 2
Cytoplasm 56 149 44 47 80 47 70 33 56 32 31 134 160 179 93 81 83 21 47 17 29 40 40
Endoplasmic Reticulum 10 39 2 3 6 12 4 5 5 6 10 8 8 6 9 15 13 0 2 4 0 2 2
Endosome 0 5 7 0 15 9 19 8 12 3 3 1 11 10 18 26 29 10 26 8 4 8 9
Golgi 0 2 0 0 1 0 0 0 0 0 2 0 1 0 10 3 3 0 3 0 0 1 1
Mitochondria 40 15 3 9 7 41 49 95 77 138 80 0 1 1 23 9 8 14 41 27 21 21 35
Nucleus 1 0 2 0 0 1 0 0 0 0 1 0 1 0 1 4 2 0 1 0 1 2 1
Nuclear Periphery 1 1 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 1 0 0
Nucleolus 0 0 0 0 0 0 0 1 0 0 0 0 0 0 1 0 1 0 0 0 0 0 0
Peroxisomes 4 6 2 3 25 24 25 11 15 0 2 0 5 0 12 20 19 4 5 7 0 2 4
SpindlePole 3 11 7 10 14 4 30 7 9 3 8 6 9 5 40 48 36 3 8 0 4 3 6
Vac/Vac Membrane 0 3 1 1 3 1 11 2 2 1 1 0 1 1 10 13 20 0 6 3 3 4 2
Unique Cell Count 137 324 82 92 160 137 202 148 167 173 130 164 218 213 255 229 210 162 433 134 147 267 264
Labelled Cell Count 192 387 97 118 197 188 262 198 211 222 164 178 247 231 343 329 287 162 433 134 147 267 264


Actin

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.7 6.0 6.5 6.0 6.6 5.6 5.8 4.9 5.3 4.4 5.1 6.1 6.3 6.2 8.0 8.4 7.9 5.7 6.2 6.5
Std Deviation (1e-4) 0.5 0.8 1.3 1.9 1.6 1.7 1.6 1.5 1.2 1.4 1.4 0.8 1.0 0.9 1.6 2.3 1.9 1.3 1.4 1.2
Intensity Change (Log2) -0.11 0.02 -0.23 -0.16 -0.41 -0.29 -0.55 -0.36 -0.09 -0.06 -0.06 0.29 0.37 0.28 -0.21 -0.08 -0.0


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 1.6 -1.4 -0.4 -3.1 -3.0 -3.2 0 -2.6 -1.9 -1.6 -4.2 1.4 0.5 -0.8
Bud 0 0.5 0.9 0.7 1.4 0.8 0 -0.4 0 -0.8 0 0.5 1.2 -0.4
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 1.4 0 1.3
Cytoplasm -0.3 -0.5 -2.8 -3.0 -4.8 -3.0 -5.7 -4.4 4.6 3.3 5.4 -2.8 -2.9 -2.2
Endoplasmic Reticulum 0 0 1.9 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0.2 -0.5 0.2 -0.9 -0.4 0 0 0 0 0 -0.4 0.7 1.2
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 1.6 0 4.7 4.1 8.9 6.7 0 8.4 0 0 0 1.6 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 3.1 3.3 2.6 0 1.9 0 0 0 0 0 0 1.9 2.0
SpindlePole 0.5 0.1 0 1.4 0 -1.0 0 -0.7 0 0 0 1.6 2.5 1.9
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 0 2.5

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration -0.5608 -0.3061 -0.3712 -0.8371 -0.3401 -0.5458 0.7214 0.6907 0.8198 0.1388 0.7976 0.7122 -0.2542 -0.5607 -0.3309 -0.4526 -0.3954 -0.3316
Actin 0.233 0.1718 0.3648 0.2453 0.2446 0.2756 0.2377 0.196 0.295 0.3948 0.2923 0.246 0.2399 0.1676 0.2862 0.2268 0.1937 0.2173
Bud 0.004 0.0053 0.0285 0.0156 0.0032 0.0038 0.0081 0.0116 0.0246 0.0103 0.0183 0.0018 0.0048 0.007 0.0232 0.0138 0.0541 0.0023
Bud Neck 0.0119 0.0146 0.0079 0.0509 0.0038 0.1911 0.0271 0.018 0.0136 0.003 0.0103 0.2073 0.0233 0.0095 0.0079 0.0006 0.0022 0.1533
Bud Periphery 0.0235 0.0136 0.087 0.051 0.018 0.016 0.0175 0.027 0.0874 0.047 0.0472 0.0055 0.005 0.0075 0.0735 0.0818 0.0714 0.0067
Bud Site 0.0377 0.1836 0.0215 0.006 0.0034 0.0053 0.082 0.2107 0.052 0.0031 0.006 0.0051 0.0408 0.1259 0.0273 0.0019 0.0057 0.0032
Cell Periphery 0.0025 0.0032 0.002 0.0018 0.001 0.0015 0.0039 0.0032 0.0017 0.0009 0.0012 0.0011 0.0023 0.0022 0.0015 0.0005 0.0011 0.0013
Cytoplasm 0.0849 0.1973 0.112 0.149 0.039 0.1254 0.0951 0.1374 0.0828 0.0764 0.0986 0.087 0.1394 0.2352 0.1403 0.192 0.1165 0.1918
Cytoplasmic Foci 0.1243 0.1038 0.0569 0.0845 0.0466 0.1183 0.154 0.0527 0.0517 0.0364 0.1069 0.0837 0.125 0.0848 0.0815 0.1 0.1782 0.1383
Eisosomes 0.0052 0.0013 0.0024 0.0026 0.0021 0.002 0.0026 0.0014 0.0015 0.0006 0.0026 0.0014 0.0021 0.0012 0.0025 0.0006 0.0013 0.0011
Endoplasmic Reticulum 0.0058 0.0024 0.0024 0.0096 0.0053 0.0023 0.0033 0.0015 0.0053 0.0013 0.0038 0.0017 0.0054 0.0025 0.0036 0.0016 0.0038 0.0033
Endosome 0.0138 0.0151 0.0118 0.0544 0.0445 0.0224 0.0344 0.004 0.018 0.0414 0.0358 0.0104 0.0181 0.0079 0.0191 0.0288 0.0055 0.0126
Golgi 0.0289 0.0048 0.0104 0.0311 0.0232 0.0152 0.0207 0.0059 0.0151 0.0237 0.0185 0.0132 0.0168 0.0088 0.0136 0.0172 0.0083 0.0135
Lipid Particles 0.0258 0.0071 0.0069 0.0036 0.0972 0.0078 0.0232 0.0021 0.0066 0.0171 0.0282 0.0047 0.0118 0.0016 0.009 0.0363 0.0042 0.0022
Mitochondria 0.0322 0.0016 0.0287 0.0342 0.0984 0.0245 0.023 0.0092 0.0277 0.0526 0.0229 0.0135 0.0101 0.002 0.0069 0.0078 0.0084 0.0045
None 0.2965 0.223 0.2097 0.2148 0.2662 0.136 0.1641 0.2668 0.222 0.1874 0.2032 0.2167 0.2814 0.2851 0.2399 0.2301 0.268 0.1958
Nuclear Periphery 0.0026 0.0012 0.0008 0.0043 0.0072 0.001 0.0046 0.0009 0.0019 0.0016 0.0017 0.002 0.0058 0.0005 0.0058 0.0012 0.0005 0.0012
Nucleolus 0.002 0.0015 0.0013 0.0004 0.0481 0.0005 0.0022 0.0006 0.0037 0.0042 0.0009 0.001 0.0014 0.0012 0.0017 0.0002 0.0003 0.0021
Nucleus 0.0021 0.0097 0.0011 0.0016 0.004 0.0007 0.0039 0.0014 0.0129 0.0276 0.0013 0.0072 0.0022 0.0016 0.0042 0.0005 0.0009 0.0028
Peroxisomes 0.0515 0.022 0.0347 0.0235 0.0286 0.0465 0.0826 0.0471 0.0672 0.0623 0.0834 0.084 0.0527 0.0343 0.0403 0.0538 0.0738 0.0373
Punctate Nuclear 0.0087 0.015 0.004 0.0012 0.006 0.0022 0.005 0.0013 0.0043 0.0023 0.0109 0.005 0.0095 0.0105 0.0097 0.0022 0.001 0.0083
Vacuole 0.0022 0.0019 0.0041 0.0096 0.0059 0.0015 0.004 0.0008 0.0039 0.004 0.0045 0.0012 0.0015 0.0029 0.0017 0.0015 0.0007 0.0008
Vacuole Periphery 0.0007 0.0002 0.0011 0.0051 0.0036 0.0006 0.0011 0.0002 0.001 0.0021 0.0017 0.0005 0.0008 0.0004 0.0008 0.0008 0.0002 0.0003

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 5.1803 7.2036 4.5817 7.3948 3.7314 6.328 9.4946 11.763 7.8815 9.7312
Translational Efficiency 1.4251 0.9885 1.2783 0.5665 1.3653 1.4966 0.9646 0.5509 0.9095 0.6704

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency
Standard name
Human Ortholog
Description Ser/Thr protein kinase; phosphorylates Pan1p-Sla1p-End3p protein complex subunits, Pan1p and Sla1p; involved in regulation of the cortical actin cytoskeleton and endocytosis; functional overlap with PRK1
Localization
Cell Percentages actin (62%)
Cell Cycle Regulation No
Subcompartmental Group N/A

Ark1

Ark1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)

Molecular Function (Gene Ontology)

Cellular Component (Gene Ontology)


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Ark1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available