Standard name
Human Ortholog
Description Putative catalytic subunit of a class II histone deacetylase complex; role in azole resistance via Hsp90p, and in the heat shock response; Hda1p interacts with the Hda2p-Hda3p subcomplex to form an active tetramer; deletion increases histone H2B, H3 and H4 acetylation; other members of the HDA1 histone deacetylase complex are Hda2p and Hda3p
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0.09 0.06 0 0.06 0.1 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.1 0.08 0 0 0 0.13 0.15 0.08 0.34 0.37 0.4 0.15 0 0 0 0 0.06 0.13 0 0 0 0 0 0
Nucleus 0.91 0.92 0.97 0.93 0.93 0.87 0.86 0.85 0.77 0.77 0.66 0.75 0.93 0.85 0.89 0.95 0.9 0.81 0.85 0.91 0.91 0.77 0.71 0.74
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.12 0.08 0 0 0 0.07 0.11 0.07 0.19 0.16 0.14 0.19 0.07 0.11 0.2 0 0.05 0.05 0.07 0 0 0.11 0.12 0.12
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0 0 0 0.07 0.08 0.09
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 1 0 0 3 2 6 2 9 12 7 7 0 0 0 0 1 0 5 2 6 0 1 0
Bud Neck 0 0 2 0 2 1 3 2 0 0 3 4 0 0 0 0 0 0 0 1 0 0 1 0
Bud Site 1 0 1 0 2 12 12 4 20 17 7 11 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 5 4 4 26 18 18 20 28 9 10 20 17 7 15 9 4 6 9 0 2 0 0 0 0
Endoplasmic Reticulum 0 0 0 2 2 0 1 0 0 0 0 0 0 0 0 2 0 2 0 0 0 0 1 0
Endosome 0 0 1 1 0 1 2 0 0 1 0 0 0 0 0 0 1 1 3 3 3 1 2 1
Golgi 0 0 1 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 3 0 1 0
Mitochondria 33 36 4 14 17 118 166 59 259 285 304 121 5 2 4 4 6 11 2 2 3 0 0 0
Nucleus 296 391 622 536 640 799 958 644 590 586 499 606 149 148 142 104 92 70 288 419 627 66 127 124
Nuclear Periphery 4 3 1 4 1 5 6 2 5 13 10 13 1 3 0 2 3 0 0 0 0 0 0 0
Nucleolus 39 36 7 8 23 65 128 51 145 122 107 153 12 20 32 4 5 4 24 9 13 9 22 19
Peroxisomes 0 0 0 0 0 0 0 0 1 0 0 0 0 2 2 0 0 0 0 0 0 0 0 2
SpindlePole 0 1 7 1 7 10 16 16 4 8 4 12 0 1 1 1 0 2 2 1 4 0 2 0
Vac/Vac Membrane 3 7 10 4 8 11 11 16 1 5 16 23 3 2 0 0 1 6 8 17 21 5 14 14
Unique Cell Count 324 426 643 576 686 915 1116 758 769 765 752 807 160 175 160 110 102 86 340 463 686 86 180 168
Labelled Cell Count 381 479 660 597 724 1042 1329 824 1043 1059 977 967 177 193 190 121 115 105 340 463 686 86 180 168


Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 6.6 7.1 7.0 6.7 6.6 5.6 5.6 6.3 5.0 5.0 5.0 5.8 7.5 7.9 7.1 10.0 9.6 7.4 7.0 7.0 7.3
Std Deviation (1e-4) 1.2 1.9 1.1 1.3 1.3 1.1 1.0 1.2 1.0 1.1 1.3 1.3 1.7 2.2 1.6 2.2 2.4 1.9 1.8 1.7 1.4
Intensity Change (Log2) – – – -0.06 -0.08 -0.32 -0.33 -0.16 -0.49 -0.5 -0.5 -0.28 0.1 0.16 0.02 0.51 0.45 0.08 -0.01 -0.01 0.05


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 3.2 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 2.5 0 0 3.8 3.4 0 2.5 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 4.4 2.9 2.2 2.0 3.8 1.1 1.3 2.9 2.4 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 2.6 2.7 8.9 9.7 6.4 15.9 17.0 17.9 9.7 0 0 0 0 0 0
Nucleus -2.9 -2.9 -6.5 -7.3 -7.4 -10.7 -10.8 -14.2 -11.4 -2.1 -6.1 -4.2 -1.1 -3.1 -6.1
Nuclear Periphery 0 0 0 0 0 0 2.9 2.5 2.8 0 0 0 0 0 0
Nucleolus 0.5 2.8 5.6 7.9 5.3 10.7 9.6 8.9 10.8 0 6.8 10.0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 -0.1 0 0.6 1.5 -1.2 -0.1 -1.2 0.7 0 0 0 0 0 0
Vacuole -1.4 -0.6 -0.6 -1.1 0.8 -3.0 -1.6 0.8 1.6 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 4.0905 3.7797 3.3781 3.4805 3.1115 3.2839 4.9701 4.9125 4.7143 4.7479 3.6414 4.1604 5.1284 4.302 3.8305 3.9789 3.7337 3.6317
Actin 0.0241 0 0.0053 0 0.0239 0.0031 0.0015 0 0.0004 0 0.0038 0.0001 0.0114 0 0.0017 0.0025 0.0003 0.0097
Bud 0.0005 0 0.0002 0 0.0008 0 0.0002 0 0.0003 0 0.0001 0 0.0001 0 0.0001 0.0002 0.0003 0
Bud Neck 0.0017 0 0.0001 0.0001 0.0011 0.0004 0.0002 0 0.0002 0.0001 0.0003 0.0005 0.0009 0 0.0001 0.0001 0.0015 0.0004
Bud Periphery 0.0005 0 0.0002 0 0.0014 0.0001 0.0003 0 0.0007 0.0001 0.0002 0.0001 0.0002 0 0.0002 0.0003 0.0013 0.0001
Bud Site 0.0039 0 0.0005 0 0.0033 0 0.001 0.0001 0.0009 0.0001 0.0007 0 0.001 0 0.0003 0.0011 0.001 0.0001
Cell Periphery 0.0001 0 0 0 0.0002 0 0.0002 0 0.0003 0 0.0001 0 0.0001 0 0.0001 0 0.0005 0
Cytoplasm 0.0047 0 0.0011 0 0.0002 0.0002 0.0018 0.0004 0.0008 0 0.0008 0 0.0017 0 0.0004 0.0006 0.0007 0
Cytoplasmic Foci 0.01 0 0.0029 0 0.0089 0.0004 0.0068 0.0059 0.0037 0 0.0066 0.0002 0.0067 0 0.0017 0.0233 0.0016 0.0007
Eisosomes 0.0002 0 0 0 0.0003 0.0001 0.0001 0 0 0 0.0001 0 0.0001 0 0 0 0 0
Endoplasmic Reticulum 0.0027 0 0.0003 0 0.0006 0.0004 0.002 0 0.0004 0 0.0007 0.0002 0.0024 0 0.0009 0.0002 0.0022 0
Endosome 0.0104 0 0.004 0 0.0148 0.0036 0.0088 0.006 0.0032 0 0.0077 0.0004 0.0064 0 0.0046 0.0054 0.0089 0.0014
Golgi 0.0039 0 0.002 0 0.0189 0.0012 0.0015 0.0005 0.0011 0 0.0015 0.0002 0.0024 0 0.0011 0.0027 0.0012 0.0015
Lipid Particles 0.0113 0 0.0022 0 0.018 0.0024 0.0042 0.0002 0.0048 0 0.0162 0.0016 0.0058 0 0.0041 0.007 0.0075 0.0023
Mitochondria 0.0024 0 0.0031 0 0.0209 0.0012 0.0105 0.0001 0.0084 0.0002 0.0011 0.005 0.0011 0.0001 0.0021 0.0024 0.0024 0.0012
None 0.0048 0 0.0018 0 0.0005 0.0005 0.0016 0.0001 0.0006 0 0.002 0 0.0007 0 0.0003 0.0008 0.0003 0
Nuclear Periphery 0.0143 0.0002 0.0021 0.0001 0.002 0.0087 0.0053 0.0002 0.0088 0.0002 0.0126 0.002 0.0053 0.0001 0.0026 0.0027 0.0084 0.0001
Nucleolus 0.0321 0.0174 0.0137 0.0347 0.1882 0.0729 0.0365 0.0189 0.0232 0.0333 0.1293 0.0779 0.024 0.0162 0.0094 0.0109 0.1727 0.0538
Nucleus 0.829 0.9821 0.9533 0.9649 0.6759 0.9021 0.9094 0.9666 0.9329 0.9658 0.7995 0.9071 0.9198 0.9834 0.9673 0.9021 0.7751 0.9263
Peroxisomes 0.0087 0 0.0007 0 0.0074 0.0006 0.0004 0.0002 0.003 0 0.0032 0.0001 0.0031 0 0.0003 0.0089 0.0004 0.0018
Punctate Nuclear 0.0321 0.0001 0.0051 0.0002 0.0104 0.0012 0.0031 0.0007 0.0017 0.0001 0.0122 0.0041 0.0058 0.0001 0.0008 0.0285 0.0033 0.0003
Vacuole 0.0015 0 0.0007 0 0.0011 0.0003 0.003 0.0001 0.0023 0 0.0008 0.0002 0.0007 0 0.0014 0.0002 0.0083 0.0001
Vacuole Periphery 0.001 0 0.0005 0 0.0013 0.0004 0.0014 0 0.0022 0 0.0006 0.0002 0.0003 0 0.0007 0.0002 0.0021 0.0001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 15.0866 15.8118 17.9446 23.6961 19.2232 11.9586 18.5735 21.898 24.9319 22.3476
Translational Efficiency 1.0256 1.0161 1.0299 0.8774 0.8013 1.1961 0.8358 0.7344 0.6551 0.7608

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✔
Translational Efficiency ✘
Standard name
Human Ortholog
Description Putative catalytic subunit of a class II histone deacetylase complex; role in azole resistance via Hsp90p, and in the heat shock response; Hda1p interacts with the Hda2p-Hda3p subcomplex to form an active tetramer; deletion increases histone H2B, H3 and H4 acetylation; other members of the HDA1 histone deacetylase complex are Hda2p and Hda3p
Localization
Cell Percentages nucleus (99%)
Cell Cycle Regulation No
Subcompartmental Group nucleus-4

Hda1

Hda1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Hda1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available