Standard name
Human Ortholog
Description Histone H4; core histone protein required for chromatin assembly and chromosome function; one of two identical histone proteins (see also HHF1); contributes to telomeric silencing; N-terminal domain involved in maintaining genomic integrity
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches – 0 0 0 0 0 – 0 0 0 0 0 – – 0 0 0 0 0 0 0 0 0 0
Bud – 0 0 0 0 0 – 0 0 0 0 0 – – 0 0 0 0 0 0 0 0 0 0
Bud Neck – 0 0 0 0 0 – 0 0 0 0 0 – – 0 0 0 0 0 0 0 0 0 0
Bud Site – 0 0 0 0 0 – 0 0 0 0 0 – – 0 0 0 0 – – – – – –
Cell Periphery – 0 0 0 0 0 – 0 0 0 0 0 – – 0 0 0 0 0 0 0 0 0 0
Cytoplasm – 0.23 0 0.11 0.17 0.05 – 0.12 0.08 0.05 0.12 0.11 – – 0 0 0.07 0 0 0 0 0 0 0
Endoplasmic Reticulum – 0 0 0 0 0 – 0 0 0 0 0 – – 0 0 0 0 0 0 0 0 0 0
Endosome – 0 0 0 0 0 – 0 0 0 0 0 – – 0 0 0 0 0 0 0 0 0 0
Golgi – 0 0 0 0 0 – 0 0 0 0 0 – – 0 0 0 0 0 0 0 0 0 0
Mitochondria – 0.23 0.25 0.38 0.27 0.25 – 0.39 0.51 0.58 0.43 0.41 – – 0.38 0.25 0.16 0.2 0 0 0 0 0 0
Nucleus – 0.77 0.85 0.7 0.65 0.79 – 0.61 0.77 0.53 0.61 0.65 – – 0.94 0.89 0.82 0.87 0.78 0.77 0.64 0.49 0.71 0.59
Nuclear Periphery – 0 0 0 0 0 – 0 0 0 0 0 – – 0 0 0 0 0 0 0 0 0.06 0
Nucleolus – 0.23 0.6 0.36 0.48 0.42 – 0.49 0.59 0.67 0.6 0.43 – – 0.56 0.2 0.26 0.21 0.12 0.08 0.3 0.48 0.17 0.3
Peroxisomes – 0 0 0 0 0 – 0 0 0 0 0 – – 0 0 0 0 0 0 0 0 0 0
SpindlePole – 0 0 0 0 0 – 0 0 0 0 0 – – 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane – 0 0 0 0 0 – 0 0 0 0 0 – – 0 0 0 0 0.05 0 0 0 0 0.06
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches – 0 0 0 0 0 – 0 0 0 0 0 – – 0 0 0 0 0 0 0 0 0 0
Bud – 0 0 0 0 0 – 0 0 0 0 0 – – 0 0 0 0 0 1 0 0 0 0
Bud Neck – 0 0 0 0 0 – 0 0 0 0 0 – – 0 0 0 0 0 0 0 0 0 0
Bud Site – 0 0 0 0 0 – 0 0 0 0 0 – – 0 0 0 0 – – – – – –
Cell Periphery – 0 0 0 0 0 – 0 0 0 0 0 – – 0 0 1 1 0 0 0 0 0 0
Cytoplasm – 6 0 6 17 5 – 23 3 4 12 10 – – 0 7 16 8 0 0 0 0 0 0
Endoplasmic Reticulum – 0 0 1 0 0 – 0 0 0 0 0 – – 0 0 1 0 0 0 0 0 0 0
Endosome – 0 0 0 1 0 – 1 0 0 0 0 – – 0 0 0 0 0 0 0 0 0 0
Golgi – 0 0 0 0 0 – 0 0 0 0 1 – – 0 0 0 0 0 0 0 0 0 0
Mitochondria – 6 5 20 28 26 – 78 20 42 42 38 – – 6 60 39 47 0 0 0 0 0 0
Nucleus – 20 17 37 66 81 – 121 30 39 60 60 – – 15 217 201 200 34 48 23 6 17 18
Nuclear Periphery – 0 0 0 0 0 – 0 0 0 0 0 – – 0 0 0 0 0 1 0 0 1 0
Nucleolus – 6 12 19 49 43 – 97 23 49 59 40 – – 9 48 64 48 5 5 10 6 4 9
Peroxisomes – 0 0 0 1 1 – 2 1 1 0 0 – – 0 0 1 0 0 0 0 0 0 0
SpindlePole – 0 0 0 0 0 – 0 0 0 0 0 – – 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane – 1 0 0 1 2 – 1 0 0 1 1 – – 0 0 1 1 2 1 0 0 0 1
Unique Cell Count 26 20 53 102 103 199 39 73 98 92 16 244 246 230 44 62 36 13 25 32
Labelled Cell Count 39 34 83 163 158 323 77 135 174 150 30 332 324 305 44 62 36 13 25 32


Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) – 165.7 58.1 109.8 105.8 101.6 – 94.7 98.4 89.3 89.5 86.2 – – 116.3 89.4 90.1 85.2 – – –
Std Deviation (1e-4) – 74.2 21.2 43.2 52.0 42.9 – 45.7 42.7 37.3 41.2 47.4 – – 38.0 34.9 37.8 36.1 – – –
Intensity Change (Log2) – – – 0.92 0.87 0.81 – 0.7 0.76 0.62 0.62 0.57 – – 1.0 0.62 0.63 0.55 – – –


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 – 0 0 0 0 0 – – 0 0 0 0
Bud 0 0 0 – 0 0 0 0 0 – – 0 0 0 0
Bud Neck 0 0 0 – 0 0 0 0 0 – – 0 0 0 0
Bud Site 0 0 0 – 0 0 0 0 0 – – 0 0 0 0
Cell Periphery 0 0 0 – 0 0 0 0 0 – – 0 0 0 0
Cytoplasm 0 0 0 – 0 0 0 0 0 – – 0 0 0 0
Endoplasmic Reticulum 0 0 0 – 0 0 0 0 0 – – 0 0 0 0
Endosome 0 0 0 – 0 0 0 0 0 – – 0 0 0 0
Golgi 0 0 0 – 0 0 0 0 0 – – 0 0 0 0
Mitochondria 1.0 0.2 0 – 1.2 1.9 2.6 1.5 1.4 – – 0 0 0 0
Nucleus -1.3 -1.8 -0.6 – -2.1 -0.7 -2.6 -2.0 -1.7 – – 0.8 0.5 -0.4 0.2
Nuclear Periphery 0 0 0 – 0 0 0 0 0 – – 0 0 0 0
Nucleolus -1.9 -1.0 -1.5 – -1.0 -0.1 0.6 0 -1.3 – – -0.2 0 -3.2 0
Peroxisomes 0 0 0 – 0 0 0 0 0 – – 0 0 0 0
SpindlePole 0 0 0 – 0 0 0 0 0 – – 0 0 0 0
Vacuole 0 0 0 – 0 0 0 0 0 – – 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration – – – – – – – – – – – – – – – – – –
Actin – – – – – – 0.0257 0.0025 0.0122 0.0071 0.0001 0.0111 – – – – – –
Bud – – – – – – 0.2545 0.0001 0.0001 0.0002 0.0008 0.0005 – – – – – –
Bud Neck – – – – – – 0.0056 0.0001 0.0001 0 0.0002 0.0023 – – – – – –
Bud Periphery – – – – – – 0.5973 0.0002 0.0001 0.0004 0.0005 0.0008 – – – – – –
Bud Site – – – – – – 0.0412 0.0002 0.0006 0.0003 0.0005 0.0003 – – – – – –
Cell Periphery – – – – – – 0.0162 0.0001 0.0004 0 0.0057 0.0006 – – – – – –
Cytoplasm – – – – – – 0.0056 0.0001 0.0003 0.0001 0.0031 0.0009 – – – – – –
Cytoplasmic Foci – – – – – – 0.0006 0.0005 0.0122 0.0024 0.038 0.0082 – – – – – –
Eisosomes – – – – – – 0.0001 0 0.0001 0 0.0009 0.0001 – – – – – –
Endoplasmic Reticulum – – – – – – 0.0019 0.0001 0.0001 0.0002 0.0001 0.0008 – – – – – –
Endosome – – – – – – 0.0017 0.0019 0.0012 0.0038 0.0004 0.0109 – – – – – –
Golgi – – – – – – 0.0008 0.0015 0.0017 0.004 0.0001 0.0074 – – – – – –
Lipid Particles – – – – – – 0.0004 0.0024 0.0041 0.0017 0.0121 0.0026 – – – – – –
Mitochondria – – – – – – 0.0023 0.0043 0.0162 0.0062 0.0447 0.0097 – – – – – –
None – – – – – – 0.0002 0.0001 0.0011 0.0001 0.0104 0.0005 – – – – – –
Nuclear Periphery – – – – – – 0.0013 0.0016 0.0024 0.0006 0.0003 0.0013 – – – – – –
Nucleolus – – – – – – 0.0013 0.0654 0.0985 0.1107 0.7003 0.3678 – – – – – –
Nucleus – – – – – – 0.0037 0.9145 0.8382 0.8586 0.1462 0.5695 – – – – – –
Peroxisomes – – – – – – 0.0004 0.0004 0.0024 0.0026 0.0026 0.0019 – – – – – –
Punctate Nuclear – – – – – – 0.0001 0.0032 0.0069 0.0007 0.0004 0.0005 – – – – – –
Vacuole – – – – – – 0.0348 0.0003 0.0005 0.0001 0.0171 0.0012 – – – – – –
Vacuole Periphery – – – – – – 0.0043 0.0004 0.0008 0.0002 0.0152 0.0011 – – – – – –

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 1888.5191 1340.4599 281.513 185.5532 1987.7255 1187.6239 750.3904 469.2468 425.131 1321.41
Translational Efficiency 1.6266 1.6215 1.181 2.2878 1.4861 1.992 1.6931 1.9225 1.3294 1.6463

Hit Data

Dataset Hit
Protein Concentration –
Protein Localization ✘
Gene Expression ✔
Translational Efficiency ✘
Standard name
Human Ortholog
Description Histone H4; core histone protein required for chromatin assembly and chromosome function; one of two identical histone proteins (see also HHF1); contributes to telomeric silencing; N-terminal domain involved in maintaining genomic integrity
Localization
Cell Percentages nucleus (53%)
Cell Cycle Regulation No
Subcompartmental Group nucleus-1

Hhf2

Hhf2


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Hhf2-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available