Standard name | |
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Human Ortholog | |
Description | Tyrosine phosphatase involved in actin organization and endocytosis; localized to the cytoplasm |
ensLOC | DeepLoc | |||||||||||||||||||||||
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Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 1 | 1 | 0 | 3 | 0 | 2 | 4 | 0 | 3 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3 | 0 | 0 | 0 |
Bud | 1 | 2 | 1 | 0 | 0 | 1 | 5 | 1 | 8 | 10 | 12 | 15 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 0 | 3 | 1 | 11 | 4 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 2 | 5 | 11 | 16 | 15 | 13 | 21 | 9 | 23 | 20 | 24 | 31 | 3 | 2 | 17 | 11 | 4 | 4 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 288 | 388 | 306 | 364 | 486 | 535 | 723 | 708 | 575 | 610 | 424 | 567 | 333 | 389 | 447 | 168 | 113 | 141 | 286 | 384 | 281 | 197 | 381 | 391 |
Endoplasmic Reticulum | 4 | 2 | 1 | 2 | 0 | 1 | 1 | 3 | 1 | 3 | 1 | 8 | 0 | 0 | 3 | 10 | 8 | 11 | 3 | 0 | 6 | 0 | 4 | 3 |
Endosome | 0 | 0 | 1 | 0 | 0 | 5 | 8 | 3 | 8 | 13 | 6 | 8 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 2 | 1 |
Golgi | 0 | 0 | 0 | 2 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 3 | 1 | 1 | 0 | 0 | 0 |
Mitochondria | 1 | 5 | 0 | 12 | 23 | 76 | 110 | 76 | 262 | 264 | 225 | 273 | 0 | 1 | 1 | 6 | 4 | 2 | 0 | 4 | 8 | 2 | 1 | 3 |
Nucleus | 1 | 1 | 2 | 0 | 1 | 0 | 2 | 5 | 2 | 2 | 1 | 2 | 0 | 0 | 2 | 3 | 1 | 2 | 0 | 2 | 0 | 0 | 1 | 1 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 3 | 0 | 6 | 0 | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 1 | 4 | 8 | 1 | 1 | 3 | 3 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 1 | 6 | 3 |
Vac/Vac Membrane | 4 | 1 | 2 | 6 | 23 | 14 | 56 | 59 | 25 | 41 | 19 | 31 | 0 | 1 | 0 | 1 | 2 | 5 | 1 | 4 | 1 | 0 | 4 | 2 |
Unique Cell Count | 294 | 395 | 310 | 375 | 520 | 596 | 847 | 813 | 764 | 822 | 607 | 798 | 334 | 389 | 451 | 174 | 117 | 144 | 302 | 403 | 315 | 208 | 424 | 419 |
Labelled Cell Count | 301 | 404 | 324 | 403 | 550 | 647 | 934 | 878 | 909 | 974 | 716 | 944 | 337 | 393 | 472 | 201 | 133 | 165 | 302 | 403 | 315 | 208 | 424 | 419 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 7.6 | 6.1 | 7.7 | 7.1 | 7.4 | 6.2 | 6.2 | 6.5 | 5.3 | 5.6 | 5.3 | 5.3 | 7.8 | 7.4 | 7.4 | 7.3 | 8.6 | 9.4 | 7.7 | 7.9 | 8.2 |
Std Deviation (1e-4) | 0.8 | 0.8 | 1.3 | 1.2 | 1.4 | 1.2 | 1.4 | 1.4 | 1.2 | 1.1 | 1.0 | 1.1 | 1.3 | 1.0 | 1.3 | 1.5 | 1.6 | 1.7 | 1.3 | 1.7 | 1.8 |
Intensity Change (Log2) | – | – | – | -0.11 | -0.05 | -0.31 | -0.31 | -0.24 | -0.53 | -0.45 | -0.54 | -0.53 | 0.03 | -0.06 | -0.05 | -0.08 | 0.17 | 0.29 | 0.01 | 0.04 | 0.09 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0.5 | -0.5 | -1.2 | -1.0 | -2.8 | -0.5 | -1.0 | 0.3 | 0.3 | -2.3 | -2.9 | 0.2 | 1.4 | 0 | 0 |
Cytoplasm | -1.5 | -3.5 | -4.9 | -6.4 | -5.9 | -9.1 | -9.4 | -10.3 | -10.1 | 1.4 | 2.2 | 0.5 | -1.6 | -1.4 | -0.6 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 3.2 | 3.8 | 6.6 | 6.7 | 5.6 | 11.9 | 11.4 | 12.3 | 11.9 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0 | 3.1 | 1.8 | 4.1 | 4.4 | 2.5 | 3.4 | 2.4 | 2.8 | 0 | 0 | 0 | 0 | 0 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 2.4467 | 3.5559 | 3.1296 | 2.4637 | 2.8608 | 3.0875 | 4.9822 | 5.9965 | 5.0042 | 4.3979 | 4.9063 | 5.2166 | 2.7274 | 3.8191 | 2.9631 | 3.0992 | 2.6063 | 3.1833 |
Actin | 0.0295 | 0.0043 | 0.0157 | 0.0612 | 0.0003 | 0.0092 | 0.035 | 0.0022 | 0.0027 | 0.0194 | 0.0005 | 0.0067 | 0.0305 | 0.0015 | 0.0063 | 0.0258 | 0.0026 | 0.0061 |
Bud | 0.0008 | 0.0057 | 0.001 | 0.0005 | 0.0002 | 0.0011 | 0.002 | 0.0036 | 0.0018 | 0.0168 | 0.0002 | 0.0006 | 0.0028 | 0.0115 | 0.0066 | 0.0031 | 0.0022 | 0.0002 |
Bud Neck | 0.0048 | 0.0001 | 0.0041 | 0.0005 | 0.0002 | 0.005 | 0.0018 | 0.0001 | 0.0006 | 0.0011 | 0.0062 | 0.0004 | 0.0021 | 0.0002 | 0.0003 | 0.001 | 0.0008 | 0.0007 |
Bud Periphery | 0.0016 | 0.0011 | 0.0009 | 0.0004 | 0.0001 | 0.0033 | 0.0042 | 0.0034 | 0.0018 | 0.0164 | 0.0002 | 0.0012 | 0.0035 | 0.0038 | 0.0027 | 0.0021 | 0.0018 | 0.0002 |
Bud Site | 0.0097 | 0.0077 | 0.009 | 0.0009 | 0.0001 | 0.001 | 0.0062 | 0.0036 | 0.0059 | 0.0041 | 0.0055 | 0.0001 | 0.0102 | 0.0201 | 0.009 | 0.0028 | 0.0045 | 0.0002 |
Cell Periphery | 0.0011 | 0.0004 | 0.0007 | 0.0003 | 0.0001 | 0.0006 | 0.0006 | 0.0003 | 0.0003 | 0.0004 | 0.0006 | 0.0001 | 0.0006 | 0.0006 | 0.0004 | 0.0003 | 0.0001 | 0.0001 |
Cytoplasm | 0.6379 | 0.8497 | 0.7914 | 0.7865 | 0.8532 | 0.8545 | 0.5714 | 0.8336 | 0.7801 | 0.755 | 0.8772 | 0.8613 | 0.6873 | 0.8545 | 0.8568 | 0.76 | 0.8856 | 0.8533 |
Cytoplasmic Foci | 0.0268 | 0.0067 | 0.014 | 0.0228 | 0.0064 | 0.008 | 0.0302 | 0.0067 | 0.018 | 0.0281 | 0.0243 | 0.0076 | 0.0336 | 0.0059 | 0.0162 | 0.0329 | 0.0089 | 0.0196 |
Eisosomes | 0.0006 | 0.0001 | 0.0011 | 0.0001 | 0 | 0.0001 | 0.0004 | 0 | 0.0002 | 0.0001 | 0.0001 | 0 | 0.0002 | 0 | 0.0001 | 0.0001 | 0 | 0 |
Endoplasmic Reticulum | 0.0125 | 0.0071 | 0.0047 | 0.003 | 0.0017 | 0.0019 | 0.0117 | 0.0024 | 0.0047 | 0.0099 | 0.0056 | 0.0021 | 0.0062 | 0.0021 | 0.0028 | 0.0027 | 0.0014 | 0.0024 |
Endosome | 0.0235 | 0.0044 | 0.0087 | 0.0296 | 0.0035 | 0.0055 | 0.0249 | 0.0022 | 0.0107 | 0.0293 | 0.0118 | 0.0043 | 0.0165 | 0.0024 | 0.0089 | 0.0391 | 0.0121 | 0.0135 |
Golgi | 0.0078 | 0.0029 | 0.0037 | 0.0086 | 0.0002 | 0.0027 | 0.0133 | 0.0004 | 0.0018 | 0.0092 | 0.0014 | 0.0005 | 0.007 | 0.0003 | 0.0027 | 0.0144 | 0.0075 | 0.0025 |
Lipid Particles | 0.0153 | 0.0016 | 0.005 | 0.0043 | 0 | 0.0004 | 0.0033 | 0.0001 | 0.0025 | 0.0023 | 0.0147 | 0.0001 | 0.0056 | 0.0002 | 0.0018 | 0.0156 | 0.0045 | 0.0054 |
Mitochondria | 0.0107 | 0.0008 | 0.0056 | 0.0032 | 0.0001 | 0.0056 | 0.0044 | 0.0003 | 0.0008 | 0.0319 | 0.0005 | 0.0009 | 0.015 | 0.0005 | 0.0045 | 0.0056 | 0.0102 | 0.005 |
None | 0.1679 | 0.099 | 0.1182 | 0.059 | 0.1314 | 0.0946 | 0.2429 | 0.137 | 0.1587 | 0.0516 | 0.0289 | 0.1123 | 0.149 | 0.0919 | 0.0745 | 0.0519 | 0.029 | 0.0767 |
Nuclear Periphery | 0.0054 | 0.0007 | 0.0007 | 0.0007 | 0.0003 | 0.0003 | 0.0221 | 0.0007 | 0.0012 | 0.0077 | 0.0007 | 0.0002 | 0.0048 | 0.0005 | 0.0006 | 0.0016 | 0.0016 | 0.0078 |
Nucleolus | 0.0032 | 0.0001 | 0.0003 | 0.0001 | 0 | 0 | 0.0003 | 0.0001 | 0.0003 | 0.0003 | 0.0063 | 0 | 0.0006 | 0.0001 | 0.0002 | 0.001 | 0.0021 | 0.0001 |
Nucleus | 0.007 | 0.0011 | 0.0009 | 0.0008 | 0.0006 | 0.0006 | 0.0097 | 0.0009 | 0.0011 | 0.0025 | 0.0023 | 0.0003 | 0.0025 | 0.0015 | 0.001 | 0.0017 | 0.0064 | 0.0006 |
Peroxisomes | 0.0188 | 0.0048 | 0.0109 | 0.0132 | 0 | 0.0042 | 0.0097 | 0.0002 | 0.0026 | 0.003 | 0.0028 | 0.0001 | 0.011 | 0.0002 | 0.0018 | 0.0255 | 0.0033 | 0.0013 |
Punctate Nuclear | 0.0044 | 0.0002 | 0.0004 | 0.0004 | 0.0002 | 0.0001 | 0.0023 | 0.0006 | 0.0018 | 0.0061 | 0.0074 | 0.0001 | 0.006 | 0.0008 | 0.0011 | 0.0043 | 0.0131 | 0.0011 |
Vacuole | 0.0092 | 0.0013 | 0.0026 | 0.0031 | 0.001 | 0.0011 | 0.0024 | 0.0012 | 0.0021 | 0.0035 | 0.0024 | 0.0008 | 0.0038 | 0.0012 | 0.0015 | 0.0071 | 0.0019 | 0.0017 |
Vacuole Periphery | 0.0012 | 0.0002 | 0.0005 | 0.0008 | 0.0002 | 0.0002 | 0.0011 | 0.0002 | 0.0003 | 0.0014 | 0.0003 | 0.0001 | 0.0013 | 0.0001 | 0.0003 | 0.0012 | 0.0005 | 0.0014 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 6.5213 | 5.8296 | 15.5731 | 28.591 | 12.6827 | 11.7318 | 14.1464 | 17.1066 | 21.597 | 20.8716 |
Translational Efficiency | 1.9 | 3.5491 | 1.296 | 1.2405 | 1.4555 | 1.1298 | 1.393 | 1.1045 | 1.3787 | 1.1079 |
Dataset | Hit |
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Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✔ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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