Standard name
Human Ortholog
Description Tyrosine phosphatase involved in actin organization and endocytosis; localized to the cytoplasm
DOWNLOAD

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0 0 0
Cytoplasm 0.98 0.98 0.99 0.97 0.93 0.9 0.85 0.87 0.75 0.74 0.7 0.71 1.0 1.0 0.99 0.97 0.97 0.98 0.95 0.95 0.89 0.95 0.9 0.93
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0.07 0.08 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0.13 0.13 0.09 0.34 0.32 0.37 0.34 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0.07 0.07 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 1 1 0 3 0 2 4 0 3 0 0 0 0 0 0 0 0 3 0 0 0
Bud 1 2 1 0 0 1 5 1 8 10 12 15 0 0 0 1 0 0 1 0 3 1 11 4
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 2 0 0 0 0 0 0 1 0 1 0 0 0
Bud Site 0 0 0 0 0 0 0 0 1 0 1 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 2 5 11 16 15 13 21 9 23 20 24 31 3 2 17 11 4 4 0 0 0 0 0 0
Cytoplasm 288 388 306 364 486 535 723 708 575 610 424 567 333 389 447 168 113 141 286 384 281 197 381 391
Endoplasmic Reticulum 4 2 1 2 0 1 1 3 1 3 1 8 0 0 3 10 8 11 3 0 6 0 4 3
Endosome 0 0 1 0 0 5 8 3 8 13 6 8 0 0 0 0 0 0 0 1 0 0 2 1
Golgi 0 0 0 2 1 0 0 0 0 0 0 0 1 0 1 0 0 0 3 1 1 0 0 0
Mitochondria 1 5 0 12 23 76 110 76 262 264 225 273 0 1 1 6 4 2 0 4 8 2 1 3
Nucleus 1 1 2 0 1 0 2 5 2 2 1 2 0 0 2 3 1 2 0 2 0 0 1 1
Nuclear Periphery 0 0 0 0 0 1 1 3 0 6 0 1 0 0 1 0 0 0 0 0 0 0 2 0
Nucleolus 0 0 0 0 0 0 0 1 1 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0
Peroxisomes 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 1 4 8 1 1 3 3 0 0 0 0 1 0 0 0 0 1 6 3
Vac/Vac Membrane 4 1 2 6 23 14 56 59 25 41 19 31 0 1 0 1 2 5 1 4 1 0 4 2
Unique Cell Count 294 395 310 375 520 596 847 813 764 822 607 798 334 389 451 174 117 144 302 403 315 208 424 419
Labelled Cell Count 301 404 324 403 550 647 934 878 909 974 716 944 337 393 472 201 133 165 302 403 315 208 424 419


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 7.6 6.1 7.7 7.1 7.4 6.2 6.2 6.5 5.3 5.6 5.3 5.3 7.8 7.4 7.4 7.3 8.6 9.4 7.7 7.9 8.2
Std Deviation (1e-4) 0.8 0.8 1.3 1.2 1.4 1.2 1.4 1.4 1.2 1.1 1.0 1.1 1.3 1.0 1.3 1.5 1.6 1.7 1.3 1.7 1.8
Intensity Change (Log2) – – – -0.11 -0.05 -0.31 -0.31 -0.24 -0.53 -0.45 -0.54 -0.53 0.03 -0.06 -0.05 -0.08 0.17 0.29 0.01 0.04 0.09


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0.5 -0.5 -1.2 -1.0 -2.8 -0.5 -1.0 0.3 0.3 -2.3 -2.9 0.2 1.4 0 0
Cytoplasm -1.5 -3.5 -4.9 -6.4 -5.9 -9.1 -9.4 -10.3 -10.1 1.4 2.2 0.5 -1.6 -1.4 -0.6
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 3.2 3.8 6.6 6.7 5.6 11.9 11.4 12.3 11.9 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 3.1 1.8 4.1 4.4 2.5 3.4 2.4 2.8 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 2.4467 3.5559 3.1296 2.4637 2.8608 3.0875 4.9822 5.9965 5.0042 4.3979 4.9063 5.2166 2.7274 3.8191 2.9631 3.0992 2.6063 3.1833
Actin 0.0295 0.0043 0.0157 0.0612 0.0003 0.0092 0.035 0.0022 0.0027 0.0194 0.0005 0.0067 0.0305 0.0015 0.0063 0.0258 0.0026 0.0061
Bud 0.0008 0.0057 0.001 0.0005 0.0002 0.0011 0.002 0.0036 0.0018 0.0168 0.0002 0.0006 0.0028 0.0115 0.0066 0.0031 0.0022 0.0002
Bud Neck 0.0048 0.0001 0.0041 0.0005 0.0002 0.005 0.0018 0.0001 0.0006 0.0011 0.0062 0.0004 0.0021 0.0002 0.0003 0.001 0.0008 0.0007
Bud Periphery 0.0016 0.0011 0.0009 0.0004 0.0001 0.0033 0.0042 0.0034 0.0018 0.0164 0.0002 0.0012 0.0035 0.0038 0.0027 0.0021 0.0018 0.0002
Bud Site 0.0097 0.0077 0.009 0.0009 0.0001 0.001 0.0062 0.0036 0.0059 0.0041 0.0055 0.0001 0.0102 0.0201 0.009 0.0028 0.0045 0.0002
Cell Periphery 0.0011 0.0004 0.0007 0.0003 0.0001 0.0006 0.0006 0.0003 0.0003 0.0004 0.0006 0.0001 0.0006 0.0006 0.0004 0.0003 0.0001 0.0001
Cytoplasm 0.6379 0.8497 0.7914 0.7865 0.8532 0.8545 0.5714 0.8336 0.7801 0.755 0.8772 0.8613 0.6873 0.8545 0.8568 0.76 0.8856 0.8533
Cytoplasmic Foci 0.0268 0.0067 0.014 0.0228 0.0064 0.008 0.0302 0.0067 0.018 0.0281 0.0243 0.0076 0.0336 0.0059 0.0162 0.0329 0.0089 0.0196
Eisosomes 0.0006 0.0001 0.0011 0.0001 0 0.0001 0.0004 0 0.0002 0.0001 0.0001 0 0.0002 0 0.0001 0.0001 0 0
Endoplasmic Reticulum 0.0125 0.0071 0.0047 0.003 0.0017 0.0019 0.0117 0.0024 0.0047 0.0099 0.0056 0.0021 0.0062 0.0021 0.0028 0.0027 0.0014 0.0024
Endosome 0.0235 0.0044 0.0087 0.0296 0.0035 0.0055 0.0249 0.0022 0.0107 0.0293 0.0118 0.0043 0.0165 0.0024 0.0089 0.0391 0.0121 0.0135
Golgi 0.0078 0.0029 0.0037 0.0086 0.0002 0.0027 0.0133 0.0004 0.0018 0.0092 0.0014 0.0005 0.007 0.0003 0.0027 0.0144 0.0075 0.0025
Lipid Particles 0.0153 0.0016 0.005 0.0043 0 0.0004 0.0033 0.0001 0.0025 0.0023 0.0147 0.0001 0.0056 0.0002 0.0018 0.0156 0.0045 0.0054
Mitochondria 0.0107 0.0008 0.0056 0.0032 0.0001 0.0056 0.0044 0.0003 0.0008 0.0319 0.0005 0.0009 0.015 0.0005 0.0045 0.0056 0.0102 0.005
None 0.1679 0.099 0.1182 0.059 0.1314 0.0946 0.2429 0.137 0.1587 0.0516 0.0289 0.1123 0.149 0.0919 0.0745 0.0519 0.029 0.0767
Nuclear Periphery 0.0054 0.0007 0.0007 0.0007 0.0003 0.0003 0.0221 0.0007 0.0012 0.0077 0.0007 0.0002 0.0048 0.0005 0.0006 0.0016 0.0016 0.0078
Nucleolus 0.0032 0.0001 0.0003 0.0001 0 0 0.0003 0.0001 0.0003 0.0003 0.0063 0 0.0006 0.0001 0.0002 0.001 0.0021 0.0001
Nucleus 0.007 0.0011 0.0009 0.0008 0.0006 0.0006 0.0097 0.0009 0.0011 0.0025 0.0023 0.0003 0.0025 0.0015 0.001 0.0017 0.0064 0.0006
Peroxisomes 0.0188 0.0048 0.0109 0.0132 0 0.0042 0.0097 0.0002 0.0026 0.003 0.0028 0.0001 0.011 0.0002 0.0018 0.0255 0.0033 0.0013
Punctate Nuclear 0.0044 0.0002 0.0004 0.0004 0.0002 0.0001 0.0023 0.0006 0.0018 0.0061 0.0074 0.0001 0.006 0.0008 0.0011 0.0043 0.0131 0.0011
Vacuole 0.0092 0.0013 0.0026 0.0031 0.001 0.0011 0.0024 0.0012 0.0021 0.0035 0.0024 0.0008 0.0038 0.0012 0.0015 0.0071 0.0019 0.0017
Vacuole Periphery 0.0012 0.0002 0.0005 0.0008 0.0002 0.0002 0.0011 0.0002 0.0003 0.0014 0.0003 0.0001 0.0013 0.0001 0.0003 0.0012 0.0005 0.0014

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 6.5213 5.8296 15.5731 28.591 12.6827 11.7318 14.1464 17.1066 21.597 20.8716
Translational Efficiency 1.9 3.5491 1.296 1.2405 1.4555 1.1298 1.393 1.1045 1.3787 1.1079

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✔
Translational Efficiency ✘
Standard name
Human Ortholog
Description Tyrosine phosphatase involved in actin organization and endocytosis; localized to the cytoplasm
Localization
Cell Percentages cytoplasm (89%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

Siw14

Siw14


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Siw14-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available