Standard name
Human Ortholog
Description Leucyl aminopeptidase yscIV with epoxide hydrolase activity; metalloenzyme containing one zinc atom; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; also known as leukotriene A4 hydrolase
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 – 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 – 0.06 0.07 0 0 0 0 0 0
Cytoplasm 0.99 0.99 0.97 0.95 0.92 0.92 0.87 – 0.8 0.73 0.71 0.71 1.0 0.99 0.96 – 0.95 0.93 0.92 0.92 0.85 0.8 0.91 0.9
Endoplasmic Reticulum 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 – 0.11 0.06 0.29 0.19 0 0 0 – 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 – 0 0 0.05 0.05 0 0 0 – 0 0 0 0 0 0.08 0 0
Nuclear Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0.06 0.05 0.06 – 0.05 0.16 0.05 0.13 0 0 0 – 0 0.07 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 – 1 4 0 1 0 0 0 – 0 0 0 2 0 0 0 1
Bud 0 0 0 0 0 1 1 – 0 1 0 1 0 0 0 – 0 0 4 3 2 0 1 5
Bud Neck 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 – 0 0 – – – – – –
Cell Periphery 0 4 2 0 0 3 4 – 9 11 2 7 0 1 1 – 6 3 0 0 0 0 0 0
Cytoplasm 139 232 89 82 173 226 234 – 172 205 141 201 60 112 134 – 88 43 130 216 100 19 83 144
Endoplasmic Reticulum 0 5 1 0 2 2 7 – 4 6 4 3 0 0 3 – 2 0 0 2 3 0 0 0
Endosome 0 0 0 1 0 0 1 – 0 0 0 0 0 1 0 – 0 0 1 2 0 0 0 1
Golgi 0 0 0 0 1 1 0 – 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Mitochondria 1 0 0 0 2 6 11 – 24 17 58 53 0 0 1 – 0 0 1 0 3 0 0 0
Nucleus 1 0 2 3 1 3 9 – 4 7 10 15 2 4 1 – 0 0 0 0 0 1 0 2
Nuclear Periphery 0 0 0 0 0 0 0 – 1 1 1 0 0 0 0 – 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 1 0 – 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 – 1 0 0 0 0 0 0 – 0 0 0 1 0 0 0 0
Vac/Vac Membrane 1 1 1 1 11 13 15 – 10 44 10 36 0 0 1 – 4 3 0 4 4 0 1 0
Unique Cell Count 141 235 92 86 188 245 270 216 280 200 284 60 113 139 93 46 142 236 119 25 92 160
Labelled Cell Count 142 242 95 87 190 256 282 226 296 226 317 62 118 141 100 49 142 236 119 25 92 160


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 8.7 13.4 10.8 10.1 11.2 9.3 9.3 – 8.4 8.5 7.4 7.4 11.6 12.4 11.7 – 21.7 19.7 – 10.7 12.3
Std Deviation (1e-4) 2.9 3.8 3.0 2.2 3.0 2.3 2.4 – 2.4 2.2 2.0 2.0 3.0 2.9 2.9 – 5.7 5.2 – 2.7 3.6
Intensity Change (Log2) – – – -0.1 0.05 -0.22 -0.23 – -0.36 -0.34 -0.55 -0.54 0.1 0.2 0.12 – 1.01 0.86 – -0.01 0.18


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 – 0 0 0 0 0 0 0 – 0 0
Bud 0 0 0 0 – 0 0 0 0 0 0 0 – 0 0
Bud Neck 0 0 0 0 – 0 0 0 0 0 0 0 – 0 0
Bud Site 0 0 0 0 – 0 0 0 0 0 0 0 – 0 0
Cell Periphery 0 0 0 0 – 0 0 0 0 0 0 0 – 0 0
Cytoplasm -0.5 -1.5 -1.5 -2.7 – -3.8 -4.8 -5.1 -5.2 1.4 1.2 -0.1 – -0.7 -0.9
Endoplasmic Reticulum 0 0 0 0 – 0 0 0 0 0 0 0 – 0 0
Endosome 0 0 0 0 – 0 0 0 0 0 0 0 – 0 0
Golgi 0 0 0 0 – 0 0 0 0 0 0 0 – 0 0
Mitochondria 0 0 0 0 – 3.3 0 5.8 4.5 0 0 0 – 0 0
Nucleus 0 0 0 0 – 0 0 0 0 0 0 0 – 0 0
Nuclear Periphery 0 0 0 0 – 0 0 0 0 0 0 0 – 0 0
Nucleolus 0 0 0 0 – 0 0 0 0 0 0 0 – 0 0
Peroxisomes 0 0 0 0 – 0 0 0 0 0 0 0 – 0 0
SpindlePole 0 0 0 0 – 0 0 0 0 0 0 0 – 0 0
Vacuole 0 0 0 0 – 0 3.7 0 3.2 0 0 0 – 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 3.5638 5.5056 4.2006 4.7559 3.3042 4.5216 4.0636 6.2163 4.8152 6.3516 4.0246 5.0067 5.4431 7.2494 6.1453 4.8386 5.0312 5.5862
Actin 0.027 0.0003 0.0103 0.0006 0.0016 0.0048 0.0096 0.0006 0.0123 0.0004 0.0017 0.0026 0.0127 0.0005 0.0026 0.0006 0.0322 0.0055
Bud 0.0004 0.0003 0.0012 0.0003 0.0008 0.0005 0.0005 0.0004 0.0004 0.0001 0.0002 0.0005 0.0011 0.0008 0.0014 0.0009 0.0008 0.0007
Bud Neck 0.0044 0.0004 0.0018 0.0005 0.0009 0.0017 0.0008 0.0003 0.0006 0.0003 0.0006 0.0013 0.0048 0.0003 0.0006 0.0009 0.0012 0.0014
Bud Periphery 0.0006 0.0001 0.0024 0.0002 0.0006 0.0003 0.0006 0.0001 0.0003 0 0.0001 0.0003 0.0012 0.0005 0.0008 0.0008 0.0008 0.0008
Bud Site 0.0069 0.0018 0.0026 0.0007 0.0011 0.0006 0.0014 0.001 0.0016 0.0001 0.0003 0.0001 0.0037 0.0018 0.0012 0.0004 0.0012 0.0003
Cell Periphery 0.0012 0.0001 0.0006 0 0.0002 0.0001 0.0004 0.0001 0.0001 0 0 0 0.0001 0 0.0001 0.0001 0.0001 0
Cytoplasm 0.841 0.9586 0.8948 0.8368 0.7589 0.9004 0.7796 0.9194 0.8687 0.8121 0.824 0.92 0.8512 0.9746 0.924 0.9127 0.8087 0.9274
Cytoplasmic Foci 0.0237 0.014 0.0147 0.0361 0.0688 0.0157 0.0428 0.0124 0.0332 0.0083 0.0405 0.0165 0.0257 0.0033 0.0135 0.0078 0.02 0.0109
Eisosomes 0.0001 0 0.0002 0 0 0 0.0005 0 0.0001 0 0 0 0.0001 0 0 0 0.0002 0
Endoplasmic Reticulum 0.0052 0.0021 0.0031 0.0022 0.0037 0.0034 0.0053 0.0086 0.0018 0.0039 0.002 0.0019 0.004 0.0011 0.0019 0.0035 0.0047 0.003
Endosome 0.0225 0.0045 0.012 0.0834 0.0328 0.013 0.0257 0.0027 0.0156 0.0109 0.0403 0.0046 0.0214 0.0017 0.0112 0.0166 0.0351 0.0112
Golgi 0.0082 0.0005 0.0033 0.0074 0.0085 0.0032 0.0132 0.0015 0.0066 0.0005 0.0562 0.0018 0.0057 0.0002 0.0033 0.001 0.0138 0.0023
Lipid Particles 0.0105 0.0002 0.004 0.0015 0.0304 0.0025 0.0089 0.0003 0.004 0.0001 0.0087 0.0003 0.0044 0 0.0006 0.0001 0.0163 0.0013
Mitochondria 0.0023 0.0003 0.0118 0.0026 0.0414 0.0018 0.0288 0.0001 0.003 0.0002 0.0056 0.0024 0.0062 0.0002 0.0023 0.004 0.02 0.0021
None 0.0241 0.0094 0.0171 0.0021 0.0203 0.0273 0.0565 0.0422 0.0327 0.1588 0.002 0.0382 0.0254 0.009 0.0202 0.0327 0.017 0.0116
Nuclear Periphery 0.0034 0.0009 0.0025 0.0012 0.0016 0.0022 0.004 0.0009 0.0007 0.0007 0.0007 0.0007 0.0058 0.0007 0.0016 0.0027 0.006 0.0022
Nucleolus 0.0001 0 0.0008 0.0001 0.0003 0.0003 0.0006 0 0.0002 0 0 0 0.0002 0 0.0001 0.0001 0.0005 0.0001
Nucleus 0.0046 0.0033 0.0077 0.0025 0.0042 0.0067 0.0059 0.0025 0.0042 0.0021 0.0025 0.0034 0.0107 0.0044 0.0095 0.0105 0.0062 0.012
Peroxisomes 0.0066 0.0002 0.0022 0.006 0.0177 0.0047 0.0064 0.006 0.0094 0.0001 0.0134 0.0034 0.0103 0.0001 0.0011 0.0004 0.0071 0.0038
Punctate Nuclear 0.0041 0.0013 0.003 0.0014 0.0032 0.0091 0.0053 0.0004 0.0037 0.0003 0.0004 0.001 0.0022 0.0002 0.0022 0.0009 0.0043 0.0014
Vacuole 0.0024 0.0014 0.0031 0.0112 0.0022 0.0011 0.0024 0.0004 0.001 0.0008 0.0005 0.0006 0.0022 0.0005 0.0014 0.0025 0.0027 0.0015
Vacuole Periphery 0.0006 0.0002 0.0007 0.0033 0.0007 0.0003 0.0009 0.0001 0.0002 0.0002 0.0003 0.0002 0.0007 0.0001 0.0003 0.0009 0.0012 0.0005

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 77.1726 42.0776 53.0071 58.4412 44.3518 69.4716 57.3857 59.4328 65.1841 55.6362
Translational Efficiency 0.788 1.1978 0.9798 1.008 0.8634 1.0035 0.9468 0.9437 0.9177 0.8466

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Leucyl aminopeptidase yscIV with epoxide hydrolase activity; metalloenzyme containing one zinc atom; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; also known as leukotriene A4 hydrolase
Localization
Cell Percentages cytoplasm (93%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

Lap2

Lap2


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Lap2-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available