ORF | |
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Human Ortholog | |
Description | Putative protein of unknown function; expression depends on Swi5p; GFP-fusion protein localizes to the endoplasmic reticulum; deletion confers sensitivity to 4-(N-(S-glutathionylacetyl)amino) phenylarsenoxide (GSAO) |
ensLOC | DeepLoc | |||||||||||||||||||||||
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Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 2 | 0 | 1 | 0 | 0 | 3 | 4 | 8 | 6 | 4 | 0 | 14 | 24 | 21 |
Bud | 0 | 1 | 0 | 0 | 0 | 0 | 1 | 5 | 3 | 3 | 11 | 14 | 3 | 1 | 1 | 1 | 0 | 0 | 2 | 0 | 0 | 7 | 18 | 23 |
Bud Neck | 0 | 0 | 0 | 3 | 6 | 3 | 6 | 4 | 3 | 3 | 3 | 7 | 1 | 0 | 1 | 2 | 5 | 2 | 2 | 2 | 0 | 9 | 12 | 16 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 4 | 2 | 2 | 5 | 1 | 2 | 1 | 2 | 3 | 2 | 2 | 3 | 2 | 3 | 2 | 6 | 5 | 7 | 4 | 0 | 2 | 20 | 22 | 31 |
Cytoplasm | 312 | 172 | 85 | 236 | 353 | 155 | 278 | 513 | 176 | 223 | 186 | 273 | 811 | 1237 | 1389 | 517 | 305 | 382 | 186 | 121 | 60 | 520 | 741 | 823 |
Endoplasmic Reticulum | 3 | 0 | 0 | 0 | 2 | 11 | 1 | 0 | 9 | 2 | 4 | 1 | 3 | 1 | 0 | 34 | 23 | 22 | 73 | 10 | 6 | 128 | 99 | 75 |
Endosome | 4 | 1 | 0 | 0 | 0 | 0 | 4 | 3 | 0 | 1 | 4 | 0 | 2 | 2 | 1 | 11 | 2 | 2 | 7 | 1 | 2 | 5 | 5 | 8 |
Golgi | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 3 | 0 | 2 | 2 | 2 |
Mitochondria | 7 | 5 | 1 | 4 | 5 | 17 | 18 | 58 | 62 | 76 | 101 | 117 | 3 | 3 | 0 | 35 | 7 | 9 | 56 | 22 | 23 | 148 | 102 | 96 |
Nucleus | 0 | 0 | 0 | 0 | 2 | 1 | 1 | 3 | 5 | 2 | 2 | 2 | 0 | 0 | 0 | 1 | 2 | 1 | 0 | 0 | 0 | 0 | 1 | 0 |
Nuclear Periphery | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 0 | 1 | 0 | 2 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 1 | 0 | 0 | 4 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 3 | 0 | 2 | 11 | 1 | 10 | 8 | 17 | 1 | 0 | 1 | 14 | 8 | 9 | 2 | 0 | 0 | 4 | 10 | 16 |
Vac/Vac Membrane | 7 | 2 | 0 | 1 | 6 | 0 | 18 | 18 | 7 | 6 | 4 | 2 | 1 | 1 | 2 | 14 | 15 | 17 | 12 | 7 | 1 | 20 | 17 | 20 |
Unique Cell Count | 327 | 180 | 86 | 243 | 364 | 176 | 305 | 579 | 241 | 300 | 287 | 373 | 823 | 1244 | 1395 | 572 | 340 | 418 | 365 | 179 | 105 | 902 | 1076 | 1157 |
Labelled Cell Count | 338 | 184 | 88 | 250 | 378 | 189 | 331 | 619 | 269 | 330 | 331 | 440 | 829 | 1251 | 1398 | 642 | 378 | 460 | 365 | 179 | 105 | 902 | 1076 | 1157 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 3.9 | 4.5 | 5.6 | 4.9 | 5.7 | 3.6 | 4.6 | 4.6 | 3.8 | 4.0 | 3.8 | 3.7 | 4.5 | 4.5 | 4.7 | 5.4 | 5.8 | 5.9 | 4.1 | 4.1 | 4.2 |
Std Deviation (1e-4) | 0.6 | 1.0 | 1.5 | 1.7 | 2.1 | 0.9 | 1.2 | 1.2 | 0.6 | 1.0 | 0.7 | 0.7 | 1.0 | 0.9 | 1.0 | 1.0 | 1.0 | 1.3 | 0.8 | 1.0 | 1.1 |
Intensity Change (Log2) | – | – | – | -0.19 | 0.01 | -0.64 | -0.3 | -0.29 | -0.58 | -0.48 | -0.56 | -0.59 | -0.31 | -0.32 | -0.27 | -0.05 | 0.05 | 0.08 | -0.45 | -0.44 | -0.41 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Actin | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud Neck | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud Site | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cell Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cyto | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endoplasmic Reticulum | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endosome | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Golgi | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Mitochondria | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nuclear Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nuc | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleolus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Peroxisomes | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
SpindlePole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Vac | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cortical Patches | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cytoplasm | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Vacuole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
R1 | R2 | R3 | ||||||||||||||||
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G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 0.8524 | 0.0467 | -0.0521 | -0.0463 | 0.284 | -0.0435 | 0.5525 | 0.5253 | 0.53 | 0.7431 | 0.2534 | 0.6648 | 2.9054 | 2.6019 | 2.6095 | 2.4679 | 2.0019 | 2.726 |
Actin | 0.0205 | 0.0008 | 0.0101 | 0.0038 | 0.051 | 0.0103 | 0.0135 | 0.0005 | 0.0115 | 0.0175 | 0.0089 | 0.0022 | 0.0127 | 0.0011 | 0.0314 | 0.0033 | 0.0047 | 0.0047 |
Bud | 0.001 | 0.0014 | 0.0022 | 0.0004 | 0.0014 | 0.0003 | 0.0003 | 0.0017 | 0.0035 | 0.0002 | 0.0003 | 0.0001 | 0.0015 | 0.036 | 0.0127 | 0.0579 | 0.0003 | 0.0093 |
Bud Neck | 0.0163 | 0.0003 | 0.0019 | 0.0009 | 0.0027 | 0.0069 | 0.0007 | 0.0002 | 0.0043 | 0.0042 | 0.0004 | 0.0007 | 0.0003 | 0.0007 | 0.0002 | 0.0004 | 0.0001 | 0.0003 |
Bud Periphery | 0.0022 | 0.0019 | 0.002 | 0.0007 | 0.0027 | 0.0008 | 0.0004 | 0.0007 | 0.0026 | 0.0003 | 0.0004 | 0.0001 | 0.0012 | 0.0088 | 0.0062 | 0.0119 | 0.0012 | 0.0038 |
Bud Site | 0.0095 | 0.0102 | 0.0072 | 0.0003 | 0.0114 | 0.0005 | 0.0024 | 0.0195 | 0.0125 | 0.0025 | 0.0012 | 0.0007 | 0.0015 | 0.0478 | 0.0066 | 0.0058 | 0.0001 | 0.0003 |
Cell Periphery | 0.0008 | 0.0005 | 0.0003 | 0.0001 | 0.0003 | 0.0002 | 0.0003 | 0.0006 | 0.0005 | 0.0002 | 0.0002 | 0.0001 | 0.0004 | 0.0018 | 0.0008 | 0.0005 | 0.0001 | 0.0001 |
Cytoplasm | 0.139 | 0.1174 | 0.1287 | 0.1123 | 0.2498 | 0.2167 | 0.2682 | 0.3335 | 0.303 | 0.3777 | 0.3403 | 0.3651 | 0.1021 | 0.1606 | 0.2269 | 0.0951 | 0.2964 | 0.3032 |
Cytoplasmic Foci | 0.0308 | 0.0073 | 0.0141 | 0.0064 | 0.0115 | 0.0198 | 0.0298 | 0.0187 | 0.0179 | 0.0166 | 0.0094 | 0.0199 | 0.0423 | 0.0162 | 0.0155 | 0.0145 | 0.0196 | 0.0273 |
Eisosomes | 0.0006 | 0.0002 | 0.0003 | 0.0002 | 0.0003 | 0.0002 | 0.0005 | 0.0002 | 0.0002 | 0.0003 | 0.0002 | 0.0001 | 0.0005 | 0.0005 | 0.0006 | 0.0003 | 0.0002 | 0.0001 |
Endoplasmic Reticulum | 0.0193 | 0.0045 | 0.0084 | 0.0051 | 0.022 | 0.0093 | 0.051 | 0.0411 | 0.0169 | 0.0248 | 0.0059 | 0.0466 | 0.0354 | 0.0024 | 0.0246 | 0.0048 | 0.0222 | 0.0277 |
Endosome | 0.0177 | 0.0022 | 0.0081 | 0.0134 | 0.0211 | 0.007 | 0.0307 | 0.0122 | 0.0253 | 0.0468 | 0.0084 | 0.0143 | 0.0338 | 0.0023 | 0.0113 | 0.0254 | 0.0018 | 0.0113 |
Golgi | 0.0053 | 0.001 | 0.0013 | 0.0009 | 0.0071 | 0.0012 | 0.0093 | 0.002 | 0.0044 | 0.0115 | 0.0079 | 0.0114 | 0.0069 | 0.0002 | 0.0094 | 0.0053 | 0.0007 | 0.0069 |
Lipid Particles | 0.0141 | 0.0016 | 0.0024 | 0.0018 | 0.0203 | 0.0034 | 0.0302 | 0.0034 | 0.0084 | 0.023 | 0.0038 | 0.0047 | 0.0361 | 0.0008 | 0.0041 | 0.0055 | 0.0024 | 0.0042 |
Mitochondria | 0.0096 | 0.001 | 0.0013 | 0.0007 | 0.0055 | 0.0012 | 0.0056 | 0.0022 | 0.0059 | 0.0115 | 0.0277 | 0.0154 | 0.0041 | 0.0023 | 0.0113 | 0.0127 | 0.0011 | 0.0162 |
None | 0.6217 | 0.8331 | 0.77 | 0.8002 | 0.5188 | 0.6956 | 0.4992 | 0.5505 | 0.5619 | 0.433 | 0.5768 | 0.5036 | 0.6846 | 0.6826 | 0.6193 | 0.7081 | 0.6119 | 0.5653 |
Nuclear Periphery | 0.0282 | 0.0017 | 0.0097 | 0.0144 | 0.0348 | 0.0077 | 0.0071 | 0.0039 | 0.003 | 0.0033 | 0.0009 | 0.0037 | 0.0076 | 0.0011 | 0.0027 | 0.0038 | 0.0012 | 0.002 |
Nucleolus | 0.003 | 0.0009 | 0.0015 | 0.0019 | 0.0054 | 0.0004 | 0.0007 | 0.0004 | 0.0005 | 0.0002 | 0.0002 | 0.0001 | 0.0015 | 0.0049 | 0.0009 | 0.0044 | 0.0001 | 0.0002 |
Nucleus | 0.0249 | 0.0027 | 0.0087 | 0.0081 | 0.0112 | 0.0029 | 0.0094 | 0.0023 | 0.0019 | 0.0012 | 0.0015 | 0.0015 | 0.0018 | 0.0136 | 0.0016 | 0.0106 | 0.0007 | 0.0015 |
Peroxisomes | 0.0111 | 0.0071 | 0.0015 | 0.0006 | 0.0026 | 0.0045 | 0.0277 | 0.0007 | 0.0079 | 0.015 | 0.0025 | 0.0064 | 0.0121 | 0.0003 | 0.0097 | 0.0014 | 0.007 | 0.0034 |
Punctate Nuclear | 0.0159 | 0.0027 | 0.0181 | 0.0265 | 0.0153 | 0.0091 | 0.0092 | 0.0024 | 0.002 | 0.0016 | 0.0011 | 0.0007 | 0.0089 | 0.0135 | 0.0018 | 0.025 | 0.0271 | 0.0106 |
Vacuole | 0.0063 | 0.0013 | 0.0018 | 0.001 | 0.0028 | 0.0013 | 0.0031 | 0.0032 | 0.0052 | 0.0055 | 0.0015 | 0.0018 | 0.0032 | 0.0022 | 0.0018 | 0.0027 | 0.0009 | 0.001 |
Vacuole Periphery | 0.0022 | 0.0001 | 0.0005 | 0.0004 | 0.0019 | 0.0006 | 0.0008 | 0.0004 | 0.0007 | 0.0033 | 0.0005 | 0.0006 | 0.0015 | 0.0003 | 0.0007 | 0.0009 | 0.0002 | 0.0005 |
R1 | R2 | |||||||||
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G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 5.3151 | 9.5027 | 18.3336 | 54.5317 | 27.5648 | 8.4994 | 6.7257 | 7.7818 | 34.5273 | 31.9874 |
Translational Efficiency | 0.778 | 0.2966 | 0.6123 | 0.4701 | 0.4634 | 0.6789 | 0.7311 | 0.6986 | 0.7359 | 0.434 |
Dataset | Hit |
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Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✔ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
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37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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