Standard name
Human Ortholog
Description Plasma membrane transporter of the major facilitator superfamily; member of the 12-spanner drug:H(+) antiporter DHA1 family; confers resistance to short-chain monocarboxylic acids and quinidine; involved in the excretion of excess amino acids; AQR1 has a paralog, QDR1, that arose from the whole genome duplication; relocalizes from plasma membrane to cytoplasm upon DNA replication stress
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0.07 0.17 0.16 0 0 0 0 0 0 0 0 0 0.13 0.08 0 0 0 0 0 0.08 0.05 0 0 0
Bud 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.07 0.05 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0.18 0.18 0.25 0 0 0 0 0 0 0 0 0 0.17 0.17 0.09 0 0 0 0.06 0.05 0.17 0.07 0 0
Cytoplasm 0.17 0.22 0.14 0.18 0.13 0.26 0.38 0.54 0.63 0.52 0.62 0.65 0.24 0.62 0.81 0.14 0.17 0.17 0 0 0 0 0 0
Endoplasmic Reticulum 0.49 0.51 0.42 0.14 0 0 0 0 0 0 0 0 0.33 0.11 0 0.07 0.05 0 0.24 0.31 0.27 0.35 0.23 0.14
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0.12 0.07 0 0 0.11 0.06 0.05 0 0 0 0 0.08 0 0 0 0 0 0 0
Nucleus 0.08 0 0 0.21 0.25 0.23 0.2 0.09 0.09 0.08 0.07 0.05 0.07 0 0 0.47 0.4 0.35 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.11 0.05 0.18 0.5 0.73 0.6 0.6 0.47 0.39 0.43 0.41 0.41 0.16 0.08 0.07 0.49 0.44 0.5 0.52 0.41 0.4 0.47 0.62 0.72
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 12 23 35 3 0 0 1 2 0 2 2 2 73 48 24 0 4 2 9 14 14 6 9 7
Bud 7 6 2 1 0 2 0 4 0 4 4 10 15 15 10 0 0 0 3 1 2 3 3 6
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 4 12 17 0 0 0 11 12 12 5 4 5
Bud Site 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 – – – – – –
Cell Periphery 32 24 56 3 0 0 0 5 4 2 9 11 97 98 68 2 6 4 14 9 44 18 9 2
Cytoplasm 31 29 30 24 30 89 161 267 183 165 295 342 136 360 641 16 26 16 5 4 2 3 6 6
Endoplasmic Reticulum 87 67 94 19 3 8 3 4 9 0 3 9 182 62 29 8 7 4 55 55 73 85 84 59
Endosome 0 1 3 5 5 3 4 5 1 6 0 5 6 5 6 2 2 1 3 2 1 0 13 13
Golgi 1 0 6 3 1 0 0 0 1 0 0 0 5 2 0 2 2 5 1 1 3 2 2 3
Mitochondria 2 2 5 0 9 40 31 12 13 34 31 26 0 0 0 1 12 1 2 0 4 4 5 7
Nucleus 15 2 5 28 56 78 83 44 26 26 35 24 37 23 9 53 61 34 0 1 0 0 0 0
Nuclear Periphery 0 0 0 0 1 0 1 1 1 3 1 1 0 0 0 0 0 0 0 0 0 0 1 3
Nucleolus 0 0 1 1 0 3 1 1 0 2 0 1 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 1 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 1
SpindlePole 1 0 2 1 0 0 0 2 0 0 0 5 25 8 26 1 1 0 0 0 0 0 0 0
Vac/Vac Membrane 20 6 40 67 165 201 253 235 113 138 197 217 91 45 52 55 67 48 119 73 108 115 231 312
Unique Cell Count 178 132 222 134 226 337 419 499 289 319 479 527 556 584 790 112 151 96 228 180 269 246 374 433
Labelled Cell Count 208 160 280 155 270 424 538 582 351 382 577 655 671 678 882 140 188 115 228 180 269 246 374 433


Cell Periphery, Vacuole

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 11.2 11.9 12.9 12.3 11.1 8.1 7.3 7.8 7.6 6.6 7.2 7.6 9.9 7.5 7.0 10.0 10.5 14.4 10.5 9.3 9.1
Std Deviation (1e-4) 2.2 2.7 3.3 2.9 3.6 3.5 3.0 3.0 2.8 2.7 2.5 2.6 1.8 1.2 2.3 2.6 3.2 4.2 2.0 1.8 2.0
Intensity Change (Log2) – – – -0.07 -0.23 -0.68 -0.82 -0.73 -0.76 -0.96 -0.84 -0.77 -0.39 -0.79 -0.88 -0.38 -0.3 0.16 -0.3 -0.47 -0.5


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches -4.0 -6.2 -7.5 -8.1 -8.6 -7.0 -6.9 -8.5 -8.9 -1.0 -3.1 -7.2 -4.4 -4.1 -3.5
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery -5.7 -8.1 -9.7 -10.8 -10.8 -8.3 -9.1 -9.9 -10.1 -2.5 -2.7 -6.7 -5.3 -5.4 -4.4
Cytoplasm 1.1 -0.1 3.6 6.6 10.1 11.3 9.1 11.9 12.8 3.4 12.2 18.8 0.2 1.0 0.7
Endoplasmic Reticulum -5.5 -10.5 -12.0 -14.0 -15.0 -11.0 -12.8 -14.9 -14.7 -2.5 -10.2 -15.6 -6.6 -8.0 -6.8
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 1.1 4.1 2.7 0.1 1.4 3.7 2.4 1.7 0 0 0 0 2.6 0
Nucleus 5.9 7.0 6.8 6.1 3.2 3.2 2.9 2.7 1.5 2.5 1.2 0 10.3 9.5 8.3
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 2.5 0 1.9 0 0 0
Vacuole 6.4 11.7 9.7 10.2 7.4 5.2 6.1 6.0 6.1 -0.6 -4.3 -5.2 5.9 5.5 5.9

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 2.1864 2.2961 2.426 1.7684 2.9625 2.2944 3.4673 3.964 3.6411 3.3717 3.1246 3.1756 1.63 3.6436 3.395 3.206 2.0313 3.0375
Actin 0.0273 0.0032 0.016 0.0163 0.0002 0.0003 0.0317 0.002 0.0072 0.0042 0.0057 0.0038 0.0002 0.0001 0.0089 0.0019 0.0001 0.0008
Bud 0.0016 0.0025 0.005 0.0007 0.0005 0.0011 0.0013 0.0019 0.004 0.0042 0.0012 0.0018 0.0002 0.0008 0.0008 0.0038 0.0004 0.0005
Bud Neck 0.0033 0.0038 0.0084 0.0023 0.0035 0.0104 0.0091 0.0072 0.0251 0.0342 0.0029 0.0352 0.0017 0.0045 0.0098 0.0023 0.0012 0.0411
Bud Periphery 0.0045 0.0053 0.0097 0.0026 0.0013 0.0066 0.0037 0.0031 0.0149 0.0462 0.0056 0.0092 0.0003 0.0012 0.0026 0.018 0.002 0.0015
Bud Site 0.0061 0.0571 0.0319 0.0036 0.0031 0.0017 0.0115 0.0942 0.0459 0.0148 0.0027 0.0019 0.0017 0.017 0.0348 0.0049 0.0012 0.0018
Cell Periphery 0.0238 0.0259 0.0134 0.0033 0.0041 0.0305 0.0611 0.028 0.0233 0.0233 0.0196 0.011 0.0027 0.0177 0.0255 0.0326 0.0059 0.0233
Cytoplasm 0.1138 0.2247 0.2047 0.1768 0.156 0.2296 0.232 0.2524 0.2312 0.1292 0.1785 0.3103 0.1165 0.2208 0.3266 0.3384 0.1603 0.2494
Cytoplasmic Foci 0.0596 0.0412 0.0452 0.0326 0.0457 0.0274 0.0808 0.0468 0.0516 0.0342 0.0844 0.0716 0.0761 0.0312 0.0494 0.0317 0.0689 0.0801
Eisosomes 0.0003 0.0002 0.0004 0.0021 0 0.0001 0.0013 0.0002 0.0002 0 0.0001 0.0001 0 0 0.0003 0 0.0001 0
Endoplasmic Reticulum 0.0307 0.0128 0.0127 0.0047 0.0078 0.0124 0.0186 0.0159 0.0184 0.0105 0.0223 0.0048 0.0156 0.0141 0.0275 0.0114 0.0083 0.0104
Endosome 0.2143 0.1419 0.1499 0.1672 0.227 0.1101 0.187 0.1479 0.1816 0.186 0.2433 0.1094 0.1733 0.1211 0.1806 0.1806 0.3507 0.1436
Golgi 0.02 0.0065 0.0117 0.0033 0.0112 0.0027 0.0145 0.0065 0.0094 0.0393 0.0087 0.0076 0.019 0.0041 0.0083 0.0057 0.0096 0.0033
Lipid Particles 0.0518 0.026 0.0332 0.0048 0.0084 0.0105 0.0648 0.0292 0.0335 0.0526 0.0527 0.0478 0.1343 0.0325 0.0409 0.1258 0.0332 0.0483
Mitochondria 0.0155 0.0025 0.0091 0.0022 0.0025 0.0044 0.0143 0.0131 0.0186 0.0044 0.0082 0.0013 0.0013 0.0006 0.0014 0.0007 0.0049 0.0005
None 0.118 0.1338 0.1321 0.2368 0.0654 0.1021 0.0571 0.1185 0.0759 0.0104 0.0099 0.1218 0.1415 0.1242 0.0808 0.0073 0.1114 0.1198
Nuclear Periphery 0.0199 0.0033 0.0055 0.0019 0.0142 0.0022 0.0125 0.0023 0.0034 0.0026 0.018 0.0008 0.0026 0.002 0.0028 0.0029 0.0014 0.0027
Nucleolus 0.0019 0.0033 0.0044 0.0011 0.0054 0.0011 0.0016 0.0022 0.0018 0.0019 0.0014 0.0011 0.0007 0.0033 0.0012 0.0006 0.0006 0.0009
Nucleus 0.0046 0.0089 0.0062 0.0033 0.0278 0.0035 0.0126 0.0034 0.0031 0.0045 0.0068 0.0023 0.0017 0.0109 0.0025 0.0019 0.0012 0.0019
Peroxisomes 0.0156 0.0018 0.0041 0.0021 0.0016 0.0009 0.0132 0.0015 0.0024 0.0016 0.0051 0.0063 0.007 0.0004 0.0139 0.0005 0.0007 0.0015
Punctate Nuclear 0.019 0.0014 0.0136 0.0005 0.0064 0.0003 0.0023 0.0007 0.0009 0.0005 0.0048 0.0009 0.0011 0.0007 0.0011 0.0005 0.0004 0.0012
Vacuole 0.238 0.2856 0.2696 0.3185 0.3954 0.4295 0.1649 0.2175 0.2378 0.3871 0.3075 0.2443 0.2973 0.3889 0.1747 0.2227 0.2317 0.2554
Vacuole Periphery 0.0104 0.0083 0.0133 0.0134 0.0124 0.0126 0.0043 0.0056 0.0097 0.0084 0.0106 0.0068 0.0052 0.0039 0.0056 0.0056 0.006 0.0118

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 16.9176 21.2847 11.2222 10.0201 18.2787 5.0099 15.7159 10.1294 8.9787 17.3223
Translational Efficiency 0.9859 0.7902 0.7846 0.6003 0.6367 1.5726 0.6518 0.5913 0.5986 0.6562

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Plasma membrane transporter of the major facilitator superfamily; member of the 12-spanner drug:H(+) antiporter DHA1 family; confers resistance to short-chain monocarboxylic acids and quinidine; involved in the excretion of excess amino acids; AQR1 has a paralog, QDR1, that arose from the whole genome duplication; relocalizes from plasma membrane to cytoplasm upon DNA replication stress
Localization
Cell Percentages cell periphery (43%)
Cell Cycle Regulation No
Subcompartmental Group cell periphery-3

Aqr1

Aqr1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Aqr1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available