Standard name
Human Ortholog
Description Cell wall protein related to glucanases; possibly involved in cell wall septation; member of the SUN family; SUN4 has a paralog, SIM1, that arose from the whole genome duplication
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0.05 0.07 0.06 0.06 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.46 0.65 0.8 0.73 0.65 0.71 0.83 0.8 0.84 0.88 0.86 0.81 0.5 0.66 0.73 0.55 0.55 0.63 0 0.11 0 0.06 0.07 0.06
Endoplasmic Reticulum 0 0.1 0 0 0.1 0.07 0 0 0 0 0 0 0.45 0.21 0.22 0 0 0 0.05 0 0.12 0.11 0.06 0
Endosome 0.08 0 0.08 0.14 0.08 0 0 0 0.06 0 0 0 0 0 0 0.09 0.09 0.06 0 0 0.05 0.05 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.16 0 0 0 0.05 0.06 0 0 0 0.06 0.18 0.11 0 0 0 0 0 0 0 0.05 0 0.06 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.37 0.26 0.19 0.11 0.16 0.14 0.15 0.18 0.14 0.1 0.05 0.08 0.05 0.11 0.05 0.53 0.5 0.45 0.78 0.77 0.7 0.61 0.77 0.81
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 1 1 2 3 2 6 4 4 2 0 0 2 4 8 8 0 1 0 2 0 5 0 1 2
Bud 0 0 0 3 3 4 8 8 8 19 10 10 0 0 4 1 0 0 0 0 1 0 2 7
Bud Neck 0 0 0 0 0 0 0 0 0 1 0 0 0 1 0 0 0 0 2 0 1 2 2 2
Bud Site 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 1 1 0 1 0 1 1 1 2 5 10 0 0 0 0 0 0 0 0 0
Cytoplasm 82 40 113 113 84 100 220 217 130 248 132 145 82 260 221 115 127 98 7 14 5 9 21 26
Endoplasmic Reticulum 4 6 5 6 13 10 5 2 3 0 1 3 73 82 66 4 5 5 12 2 18 16 19 7
Endosome 15 0 12 22 10 5 11 12 9 3 3 3 1 14 5 18 20 9 10 3 8 7 7 12
Golgi 0 1 0 0 0 0 1 0 0 0 0 0 0 0 1 1 0 1 2 0 1 0 1 0
Mitochondria 28 0 0 3 6 8 8 8 3 18 27 20 1 1 0 5 9 2 9 5 3 9 11 10
Nucleus 1 2 0 0 2 3 6 6 2 4 0 2 1 0 1 9 10 11 0 2 0 2 1 2
Nuclear Periphery 1 0 0 0 1 0 0 0 0 2 1 1 0 0 2 0 0 0 1 0 0 1 0 1
Nucleolus 1 0 0 0 0 1 0 0 0 0 0 1 0 0 0 0 1 0 0 0 0 0 0 0
Peroxisomes 0 0 0 4 0 1 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 1 0 2 4 1 1 3 2 0 1 0 0 2 4 4 0 1 1 0 0 0 0 1 1
Vac/Vac Membrane 65 16 27 17 21 20 39 48 21 28 8 14 8 45 14 111 115 70 173 99 105 92 248 363
Unique Cell Count 177 62 142 154 129 140 265 271 154 282 154 178 163 394 303 209 232 156 223 129 152 152 322 447
Labelled Cell Count 199 66 161 175 144 161 305 309 178 325 183 202 174 420 336 264 289 197 223 129 152 152 322 447


Vacuole

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.5 5.2 5.2 5.5 6.6 5.7 5.8 5.7 5.1 4.7 4.2 4.3 8.0 7.7 7.1 6.8 7.1 7.2 7.4 7.4 7.3
Std Deviation (1e-4) 0.6 0.8 1.3 1.2 2.3 1.8 2.4 2.7 1.6 1.3 0.9 1.0 1.5 1.5 1.6 1.5 1.6 2.0 1.1 1.3 1.5
Intensity Change (Log2) – – – 0.07 0.33 0.12 0.14 0.13 -0.04 -0.15 -0.32 -0.27 0.61 0.55 0.44 0.38 0.44 0.45 0.5 0.51 0.48


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm -1.3 -2.7 -1.6 0.9 0.1 1.1 2.3 1.4 0.4 -5.3 -3.0 -1.5 -4.7 -4.9 -3.2
Endoplasmic Reticulum 0.2 2.2 1.4 0 0 0 0 0 0 8.2 4.8 4.9 0 0 0
Endosome 1.6 -0.2 -1.7 -1.8 -1.7 -0.9 0 0 0 -3.4 -2.3 -3.5 0.1 0.1 -0.9
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 3.2
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole -1.9 -0.6 -1.1 -1.1 -0.3 -1.3 -2.6 -3.7 -3.0 -3.9 -2.3 -4.9 6.4 5.9 4.8

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 3.2187 3.3559 2.7122 1.8784 2.1614 3.0339 1.8777 2.5721 2.0514 2.1754 1.5364 2.4746 1.4161 2.1982 1.3364 1.7199 1.7746 2.108
Actin 0.0792 0.0001 0.0248 0 0.0001 0.0006 0.0132 0.0005 0.033 0.0244 0.0461 0.0019 0.0397 0.0062 0.0107 0.0005 0.0124 0.0013
Bud 0.0011 0.0003 0.0018 0.0002 0.0002 0.0004 0.0011 0.0004 0.0023 0.0024 0.0012 0.0006 0.0019 0.0058 0.0133 0.001 0.0008 0.0026
Bud Neck 0.0031 0.0021 0.004 0.0006 0.0005 0.0061 0.0036 0.0032 0.0039 0.0039 0.0017 0.0058 0.0026 0.0028 0.0017 0.0015 0.0016 0.0056
Bud Periphery 0.0031 0.0005 0.0025 0.0004 0.0002 0.0011 0.0022 0.0007 0.0031 0.0029 0.0019 0.0014 0.0028 0.0025 0.0034 0.0017 0.001 0.002
Bud Site 0.0034 0.0014 0.0059 0.0005 0.0006 0.0011 0.0084 0.0073 0.0117 0.0153 0.0022 0.0013 0.0072 0.0101 0.0069 0.0017 0.0014 0.0027
Cell Periphery 0.0075 0.0027 0.0022 0.0011 0.0005 0.0016 0.0016 0.0022 0.0015 0.0017 0.0006 0.0015 0.0014 0.003 0.0015 0.0011 0.0011 0.0036
Cytoplasm 0.0666 0.2032 0.1026 0.0115 0.1178 0.1092 0.0871 0.2509 0.1313 0.0469 0.0529 0.1342 0.0951 0.1096 0.1286 0.1447 0.0873 0.1216
Cytoplasmic Foci 0.0274 0.0325 0.039 0.0028 0.0217 0.0178 0.0681 0.0301 0.0312 0.0448 0.028 0.025 0.0753 0.0389 0.04 0.0455 0.0318 0.0373
Eisosomes 0.0003 0 0.0001 0 0 0 0.0002 0 0.0002 0.0002 0.0004 0 0.0002 0.0001 0.0001 0.0001 0.0001 0
Endoplasmic Reticulum 0.0995 0.0551 0.0513 0.065 0.1082 0.1808 0.0652 0.1317 0.0635 0.0701 0.041 0.16 0.1164 0.1094 0.0708 0.0655 0.0886 0.1313
Endosome 0.1957 0.18 0.3508 0.1639 0.353 0.2254 0.2874 0.1667 0.2559 0.246 0.2208 0.1823 0.2362 0.1943 0.2534 0.2129 0.2448 0.1743
Golgi 0.0145 0.0047 0.0161 0.0014 0.01 0.0072 0.0317 0.0042 0.0148 0.0249 0.0522 0.0067 0.018 0.0051 0.0099 0.0085 0.0477 0.0084
Lipid Particles 0.0319 0.0558 0.0269 0.0017 0.0082 0.0218 0.0621 0.028 0.0127 0.0445 0.0531 0.0131 0.0456 0.0217 0.012 0.0052 0.0505 0.0156
Mitochondria 0.0094 0.0009 0.0104 0.0004 0.0012 0.0067 0.0204 0.0012 0.0265 0.0216 0.0243 0.0055 0.0102 0.0104 0.0103 0.0363 0.014 0.0064
None 0.0205 0.0445 0.0382 0.0002 0.0194 0.0144 0.0236 0.0195 0.0213 0.0035 0.0008 0.0037 0.0204 0.0174 0.0136 0.013 0.0419 0.0022
Nuclear Periphery 0.0496 0.0153 0.0189 0.0197 0.0357 0.04 0.0451 0.0315 0.0318 0.0279 0.0148 0.0316 0.0357 0.0344 0.025 0.0199 0.0332 0.025
Nucleolus 0.0012 0.001 0.0034 0.0004 0.0004 0.0021 0.0037 0.0011 0.0017 0.0097 0.0015 0.001 0.0012 0.0044 0.0017 0.0122 0.0021 0.0014
Nucleus 0.0107 0.0044 0.0192 0.0022 0.0035 0.0137 0.0135 0.0083 0.0073 0.0071 0.0056 0.0099 0.0073 0.0094 0.0109 0.0158 0.0088 0.0102
Peroxisomes 0.0042 0.0003 0.0015 0.0001 0.0002 0.0006 0.0209 0.001 0.0046 0.0104 0.0168 0.0003 0.0167 0.0007 0.0056 0.001 0.0026 0.0005
Punctate Nuclear 0.0264 0.0007 0.001 0.0001 0.0006 0.0091 0.0077 0.0058 0.0017 0.0088 0.0021 0.0035 0.0062 0.0012 0.0034 0.0017 0.0032 0.002
Vacuole 0.2962 0.3752 0.2493 0.6854 0.2927 0.3023 0.2086 0.2896 0.3136 0.344 0.3897 0.3774 0.2376 0.3858 0.3385 0.3737 0.2873 0.4152
Vacuole Periphery 0.0485 0.0193 0.03 0.0426 0.0254 0.0379 0.0245 0.016 0.0266 0.0389 0.0421 0.0334 0.0223 0.027 0.0388 0.0366 0.0378 0.0308

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 61.1546 118.7088 106.0521 112.0033 239.0017 65.4866 124.7642 101.7951 119.626 242.3601
Translational Efficiency 1.031 1.0511 0.8419 0.6721 0.8991 0.6593 0.9339 0.844 0.6863 0.8428

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Cell wall protein related to glucanases; possibly involved in cell wall septation; member of the SUN family; SUN4 has a paralog, SIM1, that arose from the whole genome duplication
Localization
Cell Percentages vacuole (69%)
Cell Cycle Regulation No
Subcompartmental Group N/A

Sun4

Sun4


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Sun4-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available