Standard name | |
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Human Ortholog | |
Description | Ribonuclease H2 catalytic subunit; removes RNA primers during Okazaki fragment synthesis and errant ribonucleotides misincorporated during DNA replication; role in ribonucleotide excision repair; homolog of RNAse HI; related to human AGS4 which causes Aicardi-Goutieres syndrome |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 2 | 0 | 2 | 2 |
Bud | 1 | 0 | 0 | 0 | 2 | 2 | 4 | 5 | 9 | 4 | 5 | 5 | 0 | 0 | 0 | 0 | 0 | 0 | 11 | 11 | 6 | 2 | 2 | 4 |
Bud Neck | 0 | 0 | 3 | 1 | 4 | 5 | 3 | 5 | 5 | 9 | 3 | 7 | 0 | 2 | 0 | 0 | 0 | 0 | 3 | 1 | 2 | 1 | 1 | 3 |
Bud Site | 0 | 0 | 0 | 0 | 3 | 5 | 5 | 1 | 10 | 3 | 2 | 6 | 0 | 0 | 0 | 1 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 142 | 165 | 84 | 84 | 112 | 137 | 197 | 274 | 240 | 209 | 220 | 229 | 76 | 151 | 193 | 33 | 49 | 28 | 116 | 95 | 50 | 81 | 87 | 113 |
Endoplasmic Reticulum | 0 | 3 | 1 | 0 | 1 | 1 | 2 | 1 | 0 | 0 | 6 | 2 | 0 | 0 | 0 | 11 | 20 | 13 | 1 | 2 | 0 | 1 | 1 | 3 |
Endosome | 0 | 0 | 0 | 3 | 0 | 6 | 1 | 2 | 0 | 5 | 0 | 0 | 1 | 7 | 3 | 6 | 6 | 3 | 1 | 5 | 3 | 1 | 2 | 4 |
Golgi | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 2 | 1 | 2 | 3 | 1 | 0 | 0 | 0 |
Mitochondria | 29 | 111 | 4 | 37 | 64 | 97 | 107 | 112 | 328 | 183 | 277 | 260 | 0 | 0 | 3 | 20 | 12 | 7 | 10 | 19 | 6 | 11 | 13 | 14 |
Nucleus | 184 | 274 | 177 | 183 | 172 | 190 | 200 | 162 | 140 | 136 | 146 | 102 | 122 | 179 | 150 | 165 | 241 | 142 | 205 | 349 | 192 | 79 | 81 | 41 |
Nuclear Periphery | 0 | 1 | 1 | 1 | 0 | 0 | 2 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 2 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 1 | 2 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 2 | 1 | 2 | 2 | 1 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 1 | 3 | 3 | 4 | 2 | 2 | 10 | 18 | 5 | 13 | 10 | 4 | 0 | 1 | 1 | 1 | 5 | 0 | 23 | 32 | 11 | 6 | 9 | 11 |
Vac/Vac Membrane | 60 | 67 | 13 | 24 | 10 | 33 | 29 | 39 | 10 | 8 | 9 | 17 | 18 | 31 | 54 | 35 | 39 | 18 | 117 | 125 | 71 | 73 | 70 | 65 |
Unique Cell Count | 377 | 546 | 271 | 313 | 327 | 411 | 494 | 550 | 582 | 484 | 554 | 524 | 209 | 352 | 380 | 234 | 330 | 193 | 508 | 663 | 363 | 271 | 281 | 278 |
Labelled Cell Count | 419 | 625 | 286 | 338 | 370 | 478 | 560 | 619 | 749 | 574 | 679 | 633 | 217 | 372 | 405 | 273 | 376 | 213 | 508 | 663 | 363 | 271 | 281 | 278 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 4.2 | 3.8 | 5.1 | 4.9 | 4.4 | 4.2 | 4.2 | 4.4 | 3.4 | 3.7 | 3.4 | 3.5 | 5.1 | 5.2 | 4.8 | 5.1 | 6.2 | 6.1 | 5.4 | 5.4 | 5.8 |
Std Deviation (1e-4) | 0.6 | 0.7 | 1.0 | 1.4 | 1.3 | 0.8 | 1.1 | 1.3 | 1.1 | 0.7 | 0.5 | 0.5 | 0.8 | 1.2 | 1.0 | 1.3 | 1.7 | 1.4 | 1.8 | 1.3 | 1.8 |
Intensity Change (Log2) | – | – | – | -0.07 | -0.22 | -0.29 | -0.26 | -0.2 | -0.6 | -0.48 | -0.57 | -0.55 | -0.01 | 0.03 | -0.08 | -0.01 | 0.28 | 0.26 | 0.09 | 0.09 | 0.18 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Actin | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Bud Neck | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Bud Site | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Cell Periphery | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Cyto | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endoplasmic Reticulum | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Endosome | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Golgi | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Mitochondria | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Nuclear Periphery | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Nuc | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleolus | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Peroxisomes | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
SpindlePole | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Vac | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cortical Patches | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Cytoplasm | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Nucleus | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 |
Vacuole | – | – | – | – | – | – | – | – | – | – | – | – | 3.9 | 3.0 | 1.9 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 1.4857 | 2.1747 | 2.0779 | 1.5234 | 1.6112 | 2.1402 | 4.8234 | 4.5508 | 6.1265 | 4.7658 | 4.4231 | 4.9994 | 2.7518 | 3.0884 | 2.9528 | 2.7996 | 2.9584 | 3.0067 |
Actin | 0.0182 | 0.0003 | 0.0015 | 0.0006 | 0.0006 | 0.0002 | 0.0056 | 0 | 0.0281 | 0.0009 | 0 | 0.0005 | 0.0066 | 0 | 0.0036 | 0.0002 | 0.0001 | 0.0001 |
Bud | 0.0008 | 0.0001 | 0.0002 | 0.0001 | 0 | 0.0001 | 0.0003 | 0 | 0.0005 | 0.0002 | 0.0001 | 0.0001 | 0.0001 | 0 | 0.0001 | 0 | 0 | 0 |
Bud Neck | 0.0019 | 0.0006 | 0.0031 | 0.0009 | 0.0006 | 0.0046 | 0.0006 | 0.0002 | 0.0002 | 0.0002 | 0.0006 | 0.0233 | 0.0006 | 0.0002 | 0.0003 | 0.0002 | 0.0003 | 0.0014 |
Bud Periphery | 0.002 | 0.0002 | 0.0007 | 0.0003 | 0 | 0.0003 | 0.0003 | 0 | 0.0009 | 0.0001 | 0.0001 | 0.0001 | 0.0002 | 0 | 0.0001 | 0 | 0 | 0 |
Bud Site | 0.0067 | 0.0006 | 0.0084 | 0.0002 | 0.0001 | 0.0005 | 0.0007 | 0.0003 | 0.0011 | 0.0004 | 0.0003 | 0.0003 | 0.0006 | 0.0002 | 0.0003 | 0 | 0 | 0 |
Cell Periphery | 0.0007 | 0.0001 | 0.0002 | 0.0001 | 0 | 0.0001 | 0.0002 | 0 | 0.0001 | 0 | 0.0001 | 0.0001 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 0.0287 | 0.0136 | 0.0041 | 0.006 | 0.0112 | 0.0178 | 0.0593 | 0.022 | 0.0077 | 0.0054 | 0.001 | 0.0274 | 0.0069 | 0.0169 | 0.0049 | 0.0105 | 0.0042 | 0.011 |
Cytoplasmic Foci | 0.0029 | 0.0005 | 0.0028 | 0.0015 | 0.0017 | 0.002 | 0.0244 | 0.0007 | 0.0011 | 0.0141 | 0 | 0.0035 | 0.0044 | 0.0009 | 0.0011 | 0.0003 | 0.0003 | 0.0003 |
Eisosomes | 0.0014 | 0 | 0 | 0.0001 | 0 | 0.0001 | 0.0002 | 0 | 0.0002 | 0 | 0 | 0 | 0.0001 | 0 | 0 | 0 | 0 | 0 |
Endoplasmic Reticulum | 0.0027 | 0.0006 | 0.0008 | 0.0003 | 0.0007 | 0.0005 | 0.0047 | 0.0007 | 0.0036 | 0.0004 | 0.0001 | 0.0027 | 0.001 | 0.0005 | 0.0005 | 0.0002 | 0.0004 | 0.0003 |
Endosome | 0.0055 | 0.0008 | 0.0049 | 0.0235 | 0.0052 | 0.0042 | 0.0248 | 0.0012 | 0.0105 | 0.0036 | 0.0001 | 0.012 | 0.0069 | 0.0012 | 0.0021 | 0.0003 | 0.0004 | 0.0005 |
Golgi | 0.0031 | 0.0002 | 0.0034 | 0.0107 | 0.013 | 0.0018 | 0.0045 | 0 | 0.0061 | 0.005 | 0 | 0.0013 | 0.0047 | 0 | 0.0009 | 0 | 0 | 0 |
Lipid Particles | 0.004 | 0.0005 | 0.0059 | 0.0017 | 0.0106 | 0.0032 | 0.0213 | 0.0004 | 0.0012 | 0.061 | 0 | 0.0004 | 0.0078 | 0.0001 | 0.0017 | 0.0001 | 0.0002 | 0.0001 |
Mitochondria | 0.0171 | 0.0162 | 0.0036 | 0.0282 | 0.0156 | 0.0014 | 0.0017 | 0.0001 | 0.0049 | 0.0017 | 0.0003 | 0.0009 | 0.0021 | 0.0001 | 0.0008 | 0.0002 | 0.0004 | 0.0002 |
None | 0.0635 | 0.0141 | 0.0253 | 0.133 | 0.067 | 0.0723 | 0.1488 | 0.1752 | 0.0457 | 0.1005 | 0.0002 | 0.065 | 0.022 | 0.0364 | 0.0176 | 0.036 | 0.023 | 0.0252 |
Nuclear Periphery | 0.0097 | 0.0105 | 0.0171 | 0.0027 | 0.01 | 0.0022 | 0.0413 | 0.0043 | 0.0233 | 0.0119 | 0.004 | 0.0043 | 0.0166 | 0.0085 | 0.0088 | 0.01 | 0.0123 | 0.005 |
Nucleolus | 0.0141 | 0.0106 | 0.0098 | 0.0023 | 0.0102 | 0.0196 | 0.0038 | 0.0041 | 0.0054 | 0.0054 | 0.0138 | 0.0164 | 0.0083 | 0.0074 | 0.0073 | 0.0072 | 0.0094 | 0.0179 |
Nucleus | 0.7901 | 0.9166 | 0.8977 | 0.77 | 0.8235 | 0.8585 | 0.6348 | 0.7848 | 0.8501 | 0.7505 | 0.9765 | 0.83 | 0.8797 | 0.9076 | 0.9312 | 0.9027 | 0.9184 | 0.919 |
Peroxisomes | 0.0009 | 0.0006 | 0.0008 | 0.0004 | 0.0011 | 0.0006 | 0.0037 | 0 | 0.0004 | 0.0158 | 0 | 0.0002 | 0.0032 | 0 | 0.0026 | 0 | 0 | 0 |
Punctate Nuclear | 0.0223 | 0.0121 | 0.008 | 0.0079 | 0.0273 | 0.0083 | 0.0136 | 0.003 | 0.0034 | 0.0221 | 0.0014 | 0.0096 | 0.0271 | 0.0186 | 0.0155 | 0.0318 | 0.0302 | 0.0186 |
Vacuole | 0.0027 | 0.0009 | 0.0011 | 0.0054 | 0.0009 | 0.0016 | 0.0042 | 0.0026 | 0.002 | 0.0004 | 0.0015 | 0.0017 | 0.0007 | 0.0012 | 0.0003 | 0.0001 | 0.0001 | 0.0003 |
Vacuole Periphery | 0.0011 | 0.0004 | 0.0006 | 0.004 | 0.0005 | 0.0002 | 0.0013 | 0.0001 | 0.0036 | 0.0003 | 0.0001 | 0.0003 | 0.0004 | 0.0002 | 0.0002 | 0.0001 | 0.0001 | 0.0001 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 35.2277 | 22.4176 | 22.1798 | 19.3649 | 17.0311 | 33.418 | 15.111 | 15.6626 | 20.3443 | 28.5694 |
Translational Efficiency | 1.912 | 1.3066 | 0.9399 | 1.7625 | 3.0202 | 1.9645 | 1.3015 | 1.234 | 1.0634 | 1.3604 |
Dataset | Hit |
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Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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