| Standard name | |
|---|---|
| Human Ortholog | |
| Description | ATP-binding protein required for mismatch repair; required for both mitosis and meiosis; functions as a heterodimer with Mlh1p; binds double- and single-stranded DNA via its N-terminal domain, similar to E. coli MutL |
Micrographs
Sub-cellular Localization
Yeast GFP Assignment
Protein Abundance
Localization Change
External localization resources
| ensLOC | DeepLoc | |||||||||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
| Cortical Patches | 0 | 0 | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 2 | 6 |
| Bud | 1 | 0 | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 | 15 | 9 | 2 | 2 | 5 | 7 |
| Bud Neck | 1 | 2 | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 | 2 | 2 | 1 | 2 | 2 | 6 |
| Bud Site | 0 | 0 | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 | – | – | – | – | – | – |
| Cell Periphery | 1 | 0 | – | – | – | – | – | – | – | – | – | – | – | – | – | 1 | 0 | 1 | 0 | 0 | 1 | 0 | 1 | 0 |
| Cytoplasm | 114 | 148 | – | – | – | – | – | – | – | – | – | – | – | – | – | 68 | 52 | 64 | 69 | 109 | 65 | 43 | 66 | 45 |
| Endoplasmic Reticulum | 6 | 3 | – | – | – | – | – | – | – | – | – | – | – | – | – | 10 | 14 | 14 | 1 | 0 | 1 | 1 | 4 | 5 |
| Endosome | 2 | 0 | – | – | – | – | – | – | – | – | – | – | – | – | – | 1 | 8 | 5 | 2 | 4 | 2 | 0 | 3 | 4 |
| Golgi | 0 | 1 | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 2 | 2 | 2 | 0 | 0 | 0 | 0 | 3 |
| Mitochondria | 20 | 43 | – | – | – | – | – | – | – | – | – | – | – | – | – | 8 | 4 | 5 | 5 | 14 | 8 | 17 | 29 | 14 |
| Nucleus | 117 | 101 | – | – | – | – | – | – | – | – | – | – | – | – | – | 99 | 114 | 102 | 137 | 143 | 66 | 24 | 53 | 58 |
| Nuclear Periphery | 0 | 0 | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 | 2 | 0 | 0 | 0 | 1 | 0 |
| Nucleolus | 0 | 1 | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 3 | 3 |
| Peroxisomes | 0 | 0 | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
| SpindlePole | 0 | 1 | – | – | – | – | – | – | – | – | – | – | – | – | – | 1 | 1 | 3 | 25 | 25 | 12 | 4 | 12 | 12 |
| Vac/Vac Membrane | 39 | 58 | – | – | – | – | – | – | – | – | – | – | – | – | – | 35 | 56 | 47 | 45 | 68 | 46 | 51 | 118 | 99 |
| Unique Cell Count | 276 | 323 | 198 | 221 | 210 | 317 | 393 | 221 | 158 | 317 | 279 | |||||||||||||
| Labelled Cell Count | 301 | 358 | 223 | 251 | 244 | 317 | 393 | 221 | 158 | 317 | 279 | |||||||||||||
Yeast GFP Assignment
Protein Abundance
| Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Mean Cell GFP Intensity (1e-4) | 4.4 | 3.9 | – | – | – | – | – | – | – | – | – | – | – | – | – | 6.7 | 6.9 | 6.4 | – | – | – |
| Std Deviation (1e-4) | 0.9 | 0.6 | – | – | – | – | – | – | – | – | – | – | – | – | – | 1.8 | 2.1 | 1.9 | – | – | – |
| Intensity Change (Log2) | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Localization Change
| Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Actin | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Bud | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Bud Neck | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Bud Site | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Cell Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Cyto | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Endoplasmic Reticulum | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Endosome | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Golgi | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Mitochondria | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Nuclear Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Nuc | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Nucleolus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Peroxisomes | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| SpindlePole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Vac | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Cortical Patches | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Cytoplasm | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Nucleus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Vacuole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
External localization resources
Images
Protein Concentration and Protein Localization Data
| R1 | R2 | R3 | ||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
| Concentration | 1.7035 | 2.2834 | 2.1536 | 2.0582 | 1.6478 | 2.0619 | 0.0457 | -0.0366 | 0.1156 | -0.0766 | -0.5452 | -0.5555 | -0.3784 | 0.1605 | 0.5017 | -0.3618 | -0.5547 | -0.2577 |
| Actin | 0.0583 | 0.0006 | 0.0117 | 0.0158 | 0.0149 | 0.0028 | 0.0498 | 0.0002 | 0.011 | 0.0536 | 0.0175 | 0.003 | 0.0017 | 0.0006 | 0.0005 | 0.0005 | 0.0006 | 0.0012 |
| Bud | 0.0032 | 0.0002 | 0.0006 | 0.0001 | 0.0085 | 0.0002 | 0.0023 | 0.0002 | 0.0008 | 0.007 | 0.0005 | 0.0005 | 0.0097 | 0.0108 | 0.0038 | 0.0145 | 0.0046 | 0.0086 |
| Bud Neck | 0.0104 | 0.0019 | 0.0024 | 0.0004 | 0.0014 | 0.0037 | 0.0038 | 0.0006 | 0.0016 | 0.0075 | 0.0024 | 0.0039 | 0.0077 | 0.002 | 0.0016 | 0.002 | 0.0117 | 0.0158 |
| Bud Periphery | 0.0067 | 0.0002 | 0.0007 | 0.0004 | 0.0129 | 0.0006 | 0.0037 | 0.0002 | 0.0009 | 0.0129 | 0.0009 | 0.0005 | 0.0051 | 0.0033 | 0.0012 | 0.0057 | 0.0025 | 0.0064 |
| Bud Site | 0.0123 | 0.015 | 0.0079 | 0.0002 | 0.0092 | 0.0003 | 0.0068 | 0.003 | 0.0068 | 0.0045 | 0.0011 | 0.0002 | 0.0222 | 0.022 | 0.0045 | 0.0029 | 0.0034 | 0.0029 |
| Cell Periphery | 0.0012 | 0.0002 | 0.0004 | 0 | 0.0003 | 0.0001 | 0.0005 | 0.0001 | 0.0002 | 0.0005 | 0.0002 | 0.0001 | 0.0009 | 0.0003 | 0.0001 | 0.0002 | 0.0005 | 0.0002 |
| Cytoplasm | 0.1076 | 0.0536 | 0.0338 | 0.0399 | 0.072 | 0.084 | 0.1289 | 0.1062 | 0.0456 | 0.1248 | 0.1046 | 0.1981 | 0.0667 | 0.0315 | 0.023 | 0.0315 | 0.0642 | 0.0747 |
| Cytoplasmic Foci | 0.0165 | 0.0047 | 0.0059 | 0.0366 | 0.0221 | 0.0077 | 0.0432 | 0.0063 | 0.0102 | 0.0148 | 0.0105 | 0.0108 | 0.0247 | 0.0023 | 0.0036 | 0.0088 | 0.0145 | 0.0107 |
| Eisosomes | 0.0009 | 0.0001 | 0.0008 | 0.0002 | 0.0004 | 0.0002 | 0.0005 | 0 | 0.0001 | 0.0007 | 0.0003 | 0.0001 | 0.0002 | 0.0001 | 0.0001 | 0 | 0.0001 | 0 |
| Endoplasmic Reticulum | 0.0098 | 0.0023 | 0.0013 | 0.0008 | 0.0039 | 0.0016 | 0.0081 | 0.0038 | 0.0026 | 0.0064 | 0.0076 | 0.0037 | 0.003 | 0.0005 | 0.0006 | 0.0009 | 0.0016 | 0.0012 |
| Endosome | 0.0246 | 0.0026 | 0.0024 | 0.0016 | 0.0269 | 0.0047 | 0.0471 | 0.0042 | 0.006 | 0.0153 | 0.0243 | 0.0058 | 0.0018 | 0.0001 | 0.0011 | 0.0004 | 0.0006 | 0.0004 |
| Golgi | 0.0068 | 0.0004 | 0.0024 | 0.0021 | 0.0091 | 0.0012 | 0.0191 | 0.0001 | 0.0035 | 0.0092 | 0.0149 | 0.004 | 0.0004 | 0 | 0.0002 | 0.0001 | 0.0001 | 0.0001 |
| Lipid Particles | 0.0105 | 0.0008 | 0.0038 | 0.0087 | 0.0195 | 0.0078 | 0.0253 | 0.0019 | 0.0032 | 0.0111 | 0.0288 | 0.0027 | 0.0018 | 0.0001 | 0.0018 | 0.0002 | 0.0005 | 0.0002 |
| Mitochondria | 0.0091 | 0.0006 | 0.0123 | 0.0012 | 0.0463 | 0.0022 | 0.0143 | 0.0007 | 0.0116 | 0.0301 | 0.009 | 0.0018 | 0.0042 | 0.0003 | 0.0014 | 0.001 | 0.0005 | 0.0011 |
| None | 0.4342 | 0.3452 | 0.2217 | 0.592 | 0.6084 | 0.5807 | 0.3602 | 0.2514 | 0.1122 | 0.3066 | 0.543 | 0.3903 | 0.175 | 0.0519 | 0.0677 | 0.1918 | 0.2272 | 0.1273 |
| Nuclear Periphery | 0.022 | 0.01 | 0.0172 | 0.009 | 0.0138 | 0.0067 | 0.0308 | 0.0199 | 0.0134 | 0.0186 | 0.0162 | 0.0132 | 0.0107 | 0.0058 | 0.0076 | 0.0114 | 0.0084 | 0.0101 |
| Nucleolus | 0.0081 | 0.0098 | 0.0091 | 0.0034 | 0.0045 | 0.0092 | 0.007 | 0.0048 | 0.0049 | 0.0033 | 0.0021 | 0.0041 | 0.0256 | 0.0099 | 0.013 | 0.0121 | 0.0192 | 0.0115 |
| Nucleus | 0.2112 | 0.5091 | 0.6225 | 0.1944 | 0.0718 | 0.2484 | 0.1767 | 0.5515 | 0.7301 | 0.3409 | 0.1896 | 0.3124 | 0.2964 | 0.3734 | 0.4253 | 0.2259 | 0.2306 | 0.3248 |
| Peroxisomes | 0.0169 | 0.0005 | 0.0171 | 0.0264 | 0.0256 | 0.003 | 0.0201 | 0.0002 | 0.0105 | 0.0033 | 0.0104 | 0.0077 | 0.001 | 0.0001 | 0.0004 | 0.0002 | 0.0002 | 0.0003 |
| Punctate Nuclear | 0.016 | 0.0401 | 0.0246 | 0.0659 | 0.0253 | 0.0332 | 0.0394 | 0.041 | 0.0217 | 0.021 | 0.0116 | 0.0326 | 0.3373 | 0.4845 | 0.4415 | 0.488 | 0.4064 | 0.4014 |
| Vacuole | 0.0109 | 0.0018 | 0.0009 | 0.0005 | 0.0021 | 0.0013 | 0.0096 | 0.0031 | 0.0024 | 0.0055 | 0.0029 | 0.0037 | 0.0035 | 0.0005 | 0.0007 | 0.0013 | 0.0025 | 0.0009 |
| Vacuole Periphery | 0.0028 | 0.0002 | 0.0004 | 0.0003 | 0.001 | 0.0004 | 0.0029 | 0.0006 | 0.0006 | 0.0024 | 0.0016 | 0.0008 | 0.0006 | 0.0001 | 0.0002 | 0.0004 | 0.0002 | 0.0002 |
Sequencing Data
| R1 | R2 | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
| Gene Expression | 20.8309 | 6.0191 | 5.8622 | 13.4406 | 17.4598 | 10.5148 | 5.8006 | 5.0061 | 9.5145 | 18.9725 |
| Translational Efficiency | 0.9342 | 0.6404 | 0.8082 | 0.7905 | 0.7239 | 2.1155 | 0.8096 | 0.6658 | 0.9828 | 0.8961 |
Hit Data
| Dataset | Hit |
|---|---|
| Protein Concentration | ✘ |
| Protein Localization | ✘ |
| Gene Expression | ✔ |
| Translational Efficiency | ✘ |
| Standard name | |
|---|---|
| Human Ortholog | |
| Description | ATP-binding protein required for mismatch repair; required for both mitosis and meiosis; functions as a heterodimer with Mlh1p; binds double- and single-stranded DNA via its N-terminal domain, similar to E. coli MutL |
| Localization | |
| Cell Percentages | nucleus (48%) |
| Cell Cycle Regulation | No |
| Subcompartmental Group | nucleus-3 |
Pms1
Pms1
Nearest neighbours ( genes)
correlation threshold
0.80
Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)
Endocytosis
| Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
|---|---|---|---|---|
| 37℃ | ||||
| RT |
Cell Cycle Omics
CYCLoPs (Pms1-GFP)
| Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
|---|
| Gene | Images |
|---|
| Gene | Images |
|---|
Images are not yet available
Images are not yet available
