Standard name | |
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Human Ortholog | |
Description | EH domain-containing protein involved in endocytosis; actin cytoskeletal organization and cell wall morphogenesis; forms a complex with Sla1p and Pan1p |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 210 | 130 | 73 | 122 | 130 | 116 | 102 | 153 | 76 | 146 | 86 | 122 | 26 | 78 | 59 | 51 | 21 | 36 | 268 | 170 | 114 | 65 | 189 | 138 |
Bud | 64 | 30 | 22 | 34 | 50 | 30 | 48 | 47 | 36 | 30 | 39 | 38 | 6 | 13 | 8 | 5 | 4 | 5 | 14 | 9 | 5 | 6 | 4 | 6 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 9 | 4 | 1 | 0 | 1 | 3 |
Bud Site | 33 | 7 | 3 | 6 | 8 | 17 | 14 | 21 | 9 | 15 | 6 | 9 | 2 | 2 | 1 | 3 | 0 | 4 | – | – | – | – | – | – |
Cell Periphery | 32 | 30 | 8 | 25 | 19 | 13 | 17 | 19 | 11 | 17 | 10 | 19 | 4 | 21 | 18 | 14 | 8 | 5 | 2 | 5 | 0 | 0 | 1 | 1 |
Cytoplasm | 15 | 10 | 7 | 5 | 13 | 16 | 16 | 11 | 12 | 9 | 5 | 5 | 2 | 1 | 11 | 5 | 3 | 4 | 1 | 1 | 1 | 0 | 2 | 1 |
Endoplasmic Reticulum | 11 | 19 | 4 | 10 | 11 | 23 | 16 | 20 | 16 | 33 | 13 | 11 | 2 | 11 | 18 | 1 | 1 | 2 | 4 | 2 | 1 | 0 | 0 | 1 |
Endosome | 4 | 0 | 1 | 1 | 1 | 1 | 7 | 2 | 3 | 4 | 2 | 2 | 0 | 1 | 1 | 0 | 1 | 0 | 5 | 2 | 3 | 1 | 0 | 2 |
Golgi | 15 | 9 | 17 | 22 | 27 | 17 | 27 | 45 | 9 | 26 | 14 | 39 | 3 | 20 | 31 | 35 | 16 | 13 | 4 | 1 | 1 | 2 | 1 | 5 |
Mitochondria | 116 | 31 | 13 | 37 | 13 | 83 | 140 | 129 | 153 | 166 | 189 | 145 | 31 | 4 | 3 | 5 | 0 | 6 | 3 | 1 | 1 | 1 | 2 | 3 |
Nucleus | 1 | 1 | 0 | 0 | 2 | 1 | 1 | 1 | 3 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 1 | 0 | 0 | 0 | 0 | 1 | 1 | 3 | 2 | 4 | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 2 | 3 | 3 | 2 | 1 | 0 | 0 | 0 | 3 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 39 | 9 | 19 | 25 | 30 | 29 | 39 | 50 | 28 | 38 | 16 | 39 | 10 | 24 | 23 | 6 | 8 | 15 | 2 | 0 | 11 | 4 | 8 | 11 |
SpindlePole | 1 | 0 | 1 | 2 | 9 | 3 | 4 | 9 | 6 | 6 | 3 | 4 | 0 | 3 | 3 | 2 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vac/Vac Membrane | 3 | 1 | 1 | 0 | 1 | 2 | 4 | 2 | 4 | 1 | 1 | 2 | 1 | 0 | 0 | 0 | 2 | 0 | 1 | 0 | 0 | 0 | 0 | 0 |
Unique Cell Count | 275 | 162 | 93 | 150 | 174 | 193 | 238 | 260 | 223 | 281 | 235 | 225 | 39 | 95 | 103 | 72 | 36 | 56 | 320 | 202 | 147 | 87 | 214 | 179 |
Labelled Cell Count | 545 | 277 | 169 | 289 | 314 | 352 | 438 | 514 | 371 | 499 | 386 | 436 | 87 | 178 | 178 | 130 | 66 | 90 | 320 | 202 | 147 | 87 | 214 | 179 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 9.7 | 13.6 | 10.7 | 10.4 | 10.8 | 9.5 | 9.5 | 9.9 | 8.2 | 9.1 | 8.4 | 9.3 | 9.9 | 12.1 | 12.6 | 15.4 | 17.1 | 15.4 | 9.4 | 11.7 | 12.3 |
Std Deviation (1e-4) | 1.9 | 2.7 | 1.7 | 1.8 | 2.1 | 2.0 | 2.3 | 2.2 | 2.1 | 2.3 | 1.8 | 2.2 | 1.6 | 1.9 | 2.3 | 4.6 | 5.9 | 3.6 | 2.0 | 2.1 | 2.0 |
Intensity Change (Log2) | – | – | – | -0.04 | 0.01 | -0.17 | -0.17 | -0.11 | -0.37 | -0.23 | -0.34 | -0.2 | -0.11 | 0.18 | 0.24 | 0.53 | 0.68 | 0.53 | -0.19 | 0.13 | 0.21 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0.5 | -0.7 | -3.1 | -5.8 | -3.4 | -7.2 | -4.5 | -6.8 | -4.0 | -1.4 | 0.6 | -3.2 | -1.1 | -2.3 | -1.9 |
Bud | -0.2 | 0.9 | -1.7 | -0.7 | -1.2 | -1.6 | -3.1 | -1.5 | -1.4 | -1.1 | -1.8 | -3.1 | -2.9 | -1.6 | -2.3 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 1.7 | 0 | 1.6 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 1.8 | 0.6 | -0.6 | -0.5 | -0.4 | -1.3 | -0.9 | -1.6 | 0 | 0 | 2.6 | 1.8 | 2.0 | 0 | 0 |
Cytoplasm | 0 | 0 | 0.2 | -0.3 | 0 | -0.7 | 0 | 0 | 0 | 0 | 0 | 0.8 | -0.1 | 0 | 0 |
Endoplasmic Reticulum | 0.8 | 0.7 | 2.1 | 0.8 | 1.1 | 1.0 | 2.1 | 0 | 0 | 0 | 1.8 | 2.9 | 0 | 0 | 0 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | -0.7 | -0.6 | -2.3 | -1.7 | -0.2 | -4.2 | -2.4 | -3.4 | -0.2 | -1.5 | 0.5 | 1.9 | 4.2 | 3.1 | 0.7 |
Mitochondria | 2.0 | -1.7 | 4.9 | 7.4 | 6.0 | 8.9 | 7.5 | 11.2 | 8.2 | 7.3 | -2.3 | -2.8 | -1.4 | 0 | -0.6 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | -0.7 | -0.6 | -1.1 | -0.9 | -0.3 | -1.8 | -1.6 | -3.6 | -0.7 | 0.7 | 0.8 | 0.3 | -2.1 | 0.2 | 0.9 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 9.0177 | 8.7035 | 8.4413 | 8.4704 | 7.1387 | 9.0712 | 8.5524 | 8.0971 | 8.049 | 7.6767 | 6.5086 | 8.2675 | 6.789 | 7.0429 | 6.8963 | 6.2919 | 6.3819 | 7.6187 |
Actin | 0.8358 | 0.8171 | 0.9038 | 0.9451 | 0.8445 | 0.9069 | 0.8291 | 0.8536 | 0.8714 | 0.8922 | 0.8022 | 0.8831 | 0.7968 | 0.6926 | 0.8788 | 0.8805 | 0.8264 | 0.9188 |
Bud | 0.0007 | 0.0024 | 0.003 | 0.0046 | 0.0082 | 0.0004 | 0.0011 | 0.0054 | 0.0051 | 0.008 | 0.0062 | 0.001 | 0.0034 | 0.0018 | 0.0066 | 0.0156 | 0.0014 | 0.0014 |
Bud Neck | 0.0068 | 0.0072 | 0.0113 | 0.0035 | 0.0014 | 0.0528 | 0.0101 | 0.0052 | 0.0121 | 0.0019 | 0.0014 | 0.0711 | 0.0058 | 0.0215 | 0.0071 | 0.0036 | 0.0014 | 0.0506 |
Bud Periphery | 0.001 | 0.0028 | 0.0085 | 0.0245 | 0.0404 | 0.0012 | 0.007 | 0.0076 | 0.0225 | 0.0657 | 0.0285 | 0.0041 | 0.0064 | 0.0015 | 0.01 | 0.0547 | 0.0026 | 0.0033 |
Bud Site | 0.0221 | 0.1506 | 0.0359 | 0.0113 | 0.0035 | 0.0014 | 0.0319 | 0.0933 | 0.0463 | 0.0032 | 0.0046 | 0.0029 | 0.0332 | 0.2526 | 0.051 | 0.0029 | 0.0035 | 0.0017 |
Cell Periphery | 0.0066 | 0.001 | 0.0007 | 0.0017 | 0.0015 | 0.0007 | 0.0051 | 0.0013 | 0.0043 | 0.0083 | 0.0023 | 0.0023 | 0.0038 | 0.0013 | 0.0006 | 0.0011 | 0.0003 | 0.0005 |
Cytoplasm | 0.002 | 0.0096 | 0.0003 | 0.0005 | 0.0007 | 0.0002 | 0.0036 | 0.0056 | 0.0008 | 0.0012 | 0.0013 | 0.0007 | 0.0057 | 0.0047 | 0.0091 | 0.001 | 0.0006 | 0.0004 |
Cytoplasmic Foci | 0.0172 | 0.0048 | 0.0059 | 0.0027 | 0.0034 | 0.0022 | 0.0064 | 0.0025 | 0.0029 | 0.0003 | 0.017 | 0.0008 | 0.0246 | 0.0073 | 0.0091 | 0.0191 | 0.0488 | 0.0035 |
Eisosomes | 0.0534 | 0.0012 | 0.0019 | 0.0017 | 0.0018 | 0.0138 | 0.0766 | 0.014 | 0.013 | 0.0165 | 0.0322 | 0.0282 | 0.0131 | 0.0012 | 0.0009 | 0.0007 | 0.0009 | 0.0022 |
Endoplasmic Reticulum | 0.0006 | 0.0001 | 0.0001 | 0 | 0.0005 | 0.0001 | 0.0008 | 0.0003 | 0.0005 | 0.0002 | 0.0006 | 0.0001 | 0.0022 | 0.0002 | 0.0003 | 0.0002 | 0.0006 | 0.0001 |
Endosome | 0.0067 | 0.0002 | 0.0043 | 0.0002 | 0.0211 | 0.0018 | 0.0037 | 0.003 | 0.0033 | 0.0001 | 0.0233 | 0.0003 | 0.0156 | 0.0004 | 0.0069 | 0.0011 | 0.0165 | 0.0043 |
Golgi | 0.0142 | 0.0011 | 0.005 | 0.0016 | 0.0146 | 0.0087 | 0.0062 | 0.0038 | 0.0057 | 0.001 | 0.0362 | 0.0025 | 0.0168 | 0.0011 | 0.0067 | 0.0022 | 0.0299 | 0.0092 |
Lipid Particles | 0.0058 | 0.0001 | 0.0023 | 0.0002 | 0.0173 | 0.0007 | 0.0032 | 0.0014 | 0.0027 | 0.0001 | 0.0084 | 0.0002 | 0.0249 | 0.0005 | 0.0009 | 0.0128 | 0.0167 | 0.0007 |
Mitochondria | 0.0038 | 0.0001 | 0.0061 | 0.0004 | 0.0269 | 0.0064 | 0.0043 | 0.0011 | 0.0043 | 0.0004 | 0.0084 | 0.001 | 0.0169 | 0.0001 | 0.0044 | 0.0006 | 0.0125 | 0.0015 |
None | 0.0003 | 0.0001 | 0.0001 | 0.0001 | 0.0003 | 0 | 0.0011 | 0.0001 | 0.0006 | 0.0001 | 0.0025 | 0.0002 | 0.0041 | 0.0008 | 0.0043 | 0.0009 | 0.0004 | 0 |
Nuclear Periphery | 0.0003 | 0 | 0 | 0 | 0.0012 | 0 | 0.0005 | 0.0001 | 0.0003 | 0.0001 | 0.0046 | 0 | 0.0015 | 0.0001 | 0.0001 | 0.0001 | 0.0012 | 0.0001 |
Nucleolus | 0.0001 | 0 | 0 | 0 | 0.001 | 0 | 0.0001 | 0 | 0.0008 | 0 | 0.0016 | 0 | 0.0002 | 0 | 0.0001 | 0 | 0.0001 | 0 |
Nucleus | 0.0001 | 0.0001 | 0 | 0 | 0.001 | 0 | 0.0014 | 0.0001 | 0.0004 | 0 | 0.0025 | 0.0001 | 0.0006 | 0 | 0.0001 | 0 | 0.0001 | 0.0001 |
Peroxisomes | 0.0212 | 0.0013 | 0.0103 | 0.0017 | 0.0023 | 0.0026 | 0.0063 | 0.001 | 0.0021 | 0.001 | 0.008 | 0.001 | 0.0189 | 0.0121 | 0.002 | 0.0022 | 0.0314 | 0.0012 |
Punctate Nuclear | 0.0009 | 0.0001 | 0.0002 | 0 | 0.0003 | 0 | 0.0006 | 0.0001 | 0.0004 | 0 | 0.0062 | 0.0001 | 0.0043 | 0.0002 | 0.0003 | 0.0004 | 0.0031 | 0.0001 |
Vacuole | 0.0003 | 0 | 0.0003 | 0 | 0.0051 | 0 | 0.0008 | 0.0004 | 0.0004 | 0.0001 | 0.0011 | 0.0001 | 0.0007 | 0.0001 | 0.0004 | 0.0001 | 0.0004 | 0.0003 |
Vacuole Periphery | 0.0001 | 0 | 0.0001 | 0 | 0.0028 | 0 | 0.0002 | 0.0001 | 0.0002 | 0 | 0.0009 | 0 | 0.0005 | 0 | 0.0001 | 0.0001 | 0.0011 | 0.0001 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 47.8142 | 38.5156 | 32.0657 | 58.629 | 35.4247 | 46.2123 | 35.6187 | 53.5296 | 51.1991 | 49.8352 |
Translational Efficiency | 1.9579 | 1.7848 | 2.3521 | 1.3431 | 2.2928 | 2.5401 | 2.3495 | 1.5117 | 1.8236 | 1.8221 |
Dataset | Hit |
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Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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