Standard name
Human Ortholog
Description EH domain-containing protein involved in endocytosis; actin cytoskeletal organization and cell wall morphogenesis; forms a complex with Sla1p and Pan1p

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0.76 0.8 0.78 0.81 0.75 0.6 0.43 0.59 0.34 0.52 0.37 0.54 0.67 0.82 0.57 0.71 0.58 0.64 0.84 0.84 0.78 0.75 0.88 0.78
Bud 0.23 0.19 0.24 0.23 0.29 0.16 0.2 0.18 0.16 0.11 0.17 0.17 0.15 0.14 0.08 0.07 0.11 0.09 0.05 0.05 0 0.08 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0.12 0 0 0 0.05 0.09 0.06 0.08 0 0.05 0 0 0.05 0 0 0 0 0.07
Cell Periphery 0.12 0.19 0.09 0.17 0.11 0.07 0.07 0.07 0.05 0.06 0 0.08 0.1 0.22 0.17 0.19 0.22 0.09 0 0 0 0 0 0
Cytoplasm 0.05 0.06 0.08 0 0.07 0.08 0.07 0 0.05 0 0 0 0.05 0 0.11 0.07 0.08 0.07 0 0 0 0 0 0
Endoplasmic Reticulum 0 0.12 0 0.07 0.06 0.12 0.07 0.08 0.07 0.12 0.06 0.05 0.05 0.12 0.17 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0.05 0.06 0.18 0.15 0.16 0.09 0.11 0.17 0 0.09 0.06 0.17 0.08 0.21 0.3 0.49 0.44 0.23 0 0 0 0 0 0
Mitochondria 0.42 0.19 0.14 0.25 0.07 0.43 0.59 0.5 0.69 0.59 0.8 0.64 0.79 0 0 0.07 0 0.11 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0.14 0.06 0.2 0.17 0.17 0.15 0.16 0.19 0.13 0.14 0.07 0.17 0.26 0.25 0.22 0.08 0.22 0.27 0 0 0.07 0.06 0 0.06
SpindlePole 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 210 130 73 122 130 116 102 153 76 146 86 122 26 78 59 51 21 36 268 170 114 65 189 138
Bud 64 30 22 34 50 30 48 47 36 30 39 38 6 13 8 5 4 5 14 9 5 6 4 6
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 9 4 1 0 1 3
Bud Site 33 7 3 6 8 17 14 21 9 15 6 9 2 2 1 3 0 4
Cell Periphery 32 30 8 25 19 13 17 19 11 17 10 19 4 21 18 14 8 5 2 5 0 0 1 1
Cytoplasm 15 10 7 5 13 16 16 11 12 9 5 5 2 1 11 5 3 4 1 1 1 0 2 1
Endoplasmic Reticulum 11 19 4 10 11 23 16 20 16 33 13 11 2 11 18 1 1 2 4 2 1 0 0 1
Endosome 4 0 1 1 1 1 7 2 3 4 2 2 0 1 1 0 1 0 5 2 3 1 0 2
Golgi 15 9 17 22 27 17 27 45 9 26 14 39 3 20 31 35 16 13 4 1 1 2 1 5
Mitochondria 116 31 13 37 13 83 140 129 153 166 189 145 31 4 3 5 0 6 3 1 1 1 2 3
Nucleus 1 1 0 0 2 1 1 1 3 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 1 0 0 0 0 1 1 3 2 4 0 0 0 0 2 0 0 0 0 0 1 0 0 0
Nucleolus 0 0 0 0 0 0 2 2 3 3 2 1 0 0 0 3 0 0 0 0 0 0 0 0
Peroxisomes 39 9 19 25 30 29 39 50 28 38 16 39 10 24 23 6 8 15 2 0 11 4 8 11
SpindlePole 1 0 1 2 9 3 4 9 6 6 3 4 0 3 3 2 1 0 0 0 0 0 0 0
Vac/Vac Membrane 3 1 1 0 1 2 4 2 4 1 1 2 1 0 0 0 2 0 1 0 0 0 0 0
Unique Cell Count 275 162 93 150 174 193 238 260 223 281 235 225 39 95 103 72 36 56 320 202 147 87 214 179
Labelled Cell Count 545 277 169 289 314 352 438 514 371 499 386 436 87 178 178 130 66 90 320 202 147 87 214 179


Punctate Nuclear

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 9.7 13.6 10.7 10.4 10.8 9.5 9.5 9.9 8.2 9.1 8.4 9.3 9.9 12.1 12.6 15.4 17.1 15.4 9.4 11.7 12.3
Std Deviation (1e-4) 1.9 2.7 1.7 1.8 2.1 2.0 2.3 2.2 2.1 2.3 1.8 2.2 1.6 1.9 2.3 4.6 5.9 3.6 2.0 2.1 2.0
Intensity Change (Log2) -0.04 0.01 -0.17 -0.17 -0.11 -0.37 -0.23 -0.34 -0.2 -0.11 0.18 0.24 0.53 0.68 0.53 -0.19 0.13 0.21


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0.5 -0.7 -3.1 -5.8 -3.4 -7.2 -4.5 -6.8 -4.0 -1.4 0.6 -3.2 -1.1 -2.3 -1.9
Bud -0.2 0.9 -1.7 -0.7 -1.2 -1.6 -3.1 -1.5 -1.4 -1.1 -1.8 -3.1 -2.9 -1.6 -2.3
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 1.7 0 1.6 0 0 0 0 0 0 0 0 0 0
Cell Periphery 1.8 0.6 -0.6 -0.5 -0.4 -1.3 -0.9 -1.6 0 0 2.6 1.8 2.0 0 0
Cytoplasm 0 0 0.2 -0.3 0 -0.7 0 0 0 0 0 0.8 -0.1 0 0
Endoplasmic Reticulum 0.8 0.7 2.1 0.8 1.1 1.0 2.1 0 0 0 1.8 2.9 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi -0.7 -0.6 -2.3 -1.7 -0.2 -4.2 -2.4 -3.4 -0.2 -1.5 0.5 1.9 4.2 3.1 0.7
Mitochondria 2.0 -1.7 4.9 7.4 6.0 8.9 7.5 11.2 8.2 7.3 -2.3 -2.8 -1.4 0 -0.6
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes -0.7 -0.6 -1.1 -0.9 -0.3 -1.8 -1.6 -3.6 -0.7 0.7 0.8 0.3 -2.1 0.2 0.9
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 9.0177 8.7035 8.4413 8.4704 7.1387 9.0712 8.5524 8.0971 8.049 7.6767 6.5086 8.2675 6.789 7.0429 6.8963 6.2919 6.3819 7.6187
Actin 0.8358 0.8171 0.9038 0.9451 0.8445 0.9069 0.8291 0.8536 0.8714 0.8922 0.8022 0.8831 0.7968 0.6926 0.8788 0.8805 0.8264 0.9188
Bud 0.0007 0.0024 0.003 0.0046 0.0082 0.0004 0.0011 0.0054 0.0051 0.008 0.0062 0.001 0.0034 0.0018 0.0066 0.0156 0.0014 0.0014
Bud Neck 0.0068 0.0072 0.0113 0.0035 0.0014 0.0528 0.0101 0.0052 0.0121 0.0019 0.0014 0.0711 0.0058 0.0215 0.0071 0.0036 0.0014 0.0506
Bud Periphery 0.001 0.0028 0.0085 0.0245 0.0404 0.0012 0.007 0.0076 0.0225 0.0657 0.0285 0.0041 0.0064 0.0015 0.01 0.0547 0.0026 0.0033
Bud Site 0.0221 0.1506 0.0359 0.0113 0.0035 0.0014 0.0319 0.0933 0.0463 0.0032 0.0046 0.0029 0.0332 0.2526 0.051 0.0029 0.0035 0.0017
Cell Periphery 0.0066 0.001 0.0007 0.0017 0.0015 0.0007 0.0051 0.0013 0.0043 0.0083 0.0023 0.0023 0.0038 0.0013 0.0006 0.0011 0.0003 0.0005
Cytoplasm 0.002 0.0096 0.0003 0.0005 0.0007 0.0002 0.0036 0.0056 0.0008 0.0012 0.0013 0.0007 0.0057 0.0047 0.0091 0.001 0.0006 0.0004
Cytoplasmic Foci 0.0172 0.0048 0.0059 0.0027 0.0034 0.0022 0.0064 0.0025 0.0029 0.0003 0.017 0.0008 0.0246 0.0073 0.0091 0.0191 0.0488 0.0035
Eisosomes 0.0534 0.0012 0.0019 0.0017 0.0018 0.0138 0.0766 0.014 0.013 0.0165 0.0322 0.0282 0.0131 0.0012 0.0009 0.0007 0.0009 0.0022
Endoplasmic Reticulum 0.0006 0.0001 0.0001 0 0.0005 0.0001 0.0008 0.0003 0.0005 0.0002 0.0006 0.0001 0.0022 0.0002 0.0003 0.0002 0.0006 0.0001
Endosome 0.0067 0.0002 0.0043 0.0002 0.0211 0.0018 0.0037 0.003 0.0033 0.0001 0.0233 0.0003 0.0156 0.0004 0.0069 0.0011 0.0165 0.0043
Golgi 0.0142 0.0011 0.005 0.0016 0.0146 0.0087 0.0062 0.0038 0.0057 0.001 0.0362 0.0025 0.0168 0.0011 0.0067 0.0022 0.0299 0.0092
Lipid Particles 0.0058 0.0001 0.0023 0.0002 0.0173 0.0007 0.0032 0.0014 0.0027 0.0001 0.0084 0.0002 0.0249 0.0005 0.0009 0.0128 0.0167 0.0007
Mitochondria 0.0038 0.0001 0.0061 0.0004 0.0269 0.0064 0.0043 0.0011 0.0043 0.0004 0.0084 0.001 0.0169 0.0001 0.0044 0.0006 0.0125 0.0015
None 0.0003 0.0001 0.0001 0.0001 0.0003 0 0.0011 0.0001 0.0006 0.0001 0.0025 0.0002 0.0041 0.0008 0.0043 0.0009 0.0004 0
Nuclear Periphery 0.0003 0 0 0 0.0012 0 0.0005 0.0001 0.0003 0.0001 0.0046 0 0.0015 0.0001 0.0001 0.0001 0.0012 0.0001
Nucleolus 0.0001 0 0 0 0.001 0 0.0001 0 0.0008 0 0.0016 0 0.0002 0 0.0001 0 0.0001 0
Nucleus 0.0001 0.0001 0 0 0.001 0 0.0014 0.0001 0.0004 0 0.0025 0.0001 0.0006 0 0.0001 0 0.0001 0.0001
Peroxisomes 0.0212 0.0013 0.0103 0.0017 0.0023 0.0026 0.0063 0.001 0.0021 0.001 0.008 0.001 0.0189 0.0121 0.002 0.0022 0.0314 0.0012
Punctate Nuclear 0.0009 0.0001 0.0002 0 0.0003 0 0.0006 0.0001 0.0004 0 0.0062 0.0001 0.0043 0.0002 0.0003 0.0004 0.0031 0.0001
Vacuole 0.0003 0 0.0003 0 0.0051 0 0.0008 0.0004 0.0004 0.0001 0.0011 0.0001 0.0007 0.0001 0.0004 0.0001 0.0004 0.0003
Vacuole Periphery 0.0001 0 0.0001 0 0.0028 0 0.0002 0.0001 0.0002 0 0.0009 0 0.0005 0 0.0001 0.0001 0.0011 0.0001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 47.8142 38.5156 32.0657 58.629 35.4247 46.2123 35.6187 53.5296 51.1991 49.8352
Translational Efficiency 1.9579 1.7848 2.3521 1.3431 2.2928 2.5401 2.3495 1.5117 1.8236 1.8221

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency
Standard name
Human Ortholog
Description EH domain-containing protein involved in endocytosis; actin cytoskeletal organization and cell wall morphogenesis; forms a complex with Sla1p and Pan1p
Localization
Cell Percentages actin (98%)
Cell Cycle Regulation No
Subcompartmental Group N/A

End3

End3


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)

Molecular Function (Gene Ontology)

Cellular Component (Gene Ontology)


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (End3-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available