Standard name
Human Ortholog
Description Protein component of the small (40S) ribosomal subunit; interacts with Kti11p; deletion causes hypersensitivity to zymocin; homologous to mammalian ribosomal protein S7, no bacterial homolog; RPS7B has a paralog, RPS7A, that arose from the whole genome duplication; protein abundance increases in response to DNA replication stress
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0.1 0 0.07 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0.05 0 0.07 0.05 0.09 0.06 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.99 0.98 1.0 0.99 0.93 0.87 0.82 0.77 0.76 0.7 0.76 0.72 0.97 0.98 0.98 0.98 0.98 0.98 0.99 0.96 0.93 0.87 0.91 0.85
Endoplasmic Reticulum 0 0 0 0 0 0.08 0.12 0.15 0.14 0.17 0.16 0.12 0 0 0 0.08 0 0.05 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0.05 0 0 0 0.07 0.1 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0.05 0 0 0.08 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 1 1 5 7 8 20 6 15 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 1 1 0 0 1 0 0 0 0 0 0 0 2 0 0 0 0 3 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 2 4 1 5 8 8 17 11 18 11 6 1 1 1 0 5 1 0 0 0 0 2 1
Cytoplasm 257 409 151 110 111 148 150 196 158 139 136 157 119 171 138 131 271 294 251 390 139 109 113 114
Endoplasmic Reticulum 2 5 0 1 1 13 22 39 28 33 28 27 3 3 1 11 11 15 0 2 4 4 2 3
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1
Golgi 0 0 0 0 2 3 9 5 9 5 13 21 0 0 0 0 0 1 0 0 0 0 0 1
Mitochondria 0 0 0 0 0 0 0 1 11 4 3 17 0 0 0 2 2 0 0 0 0 1 0 0
Nucleus 0 1 0 0 1 0 0 0 0 0 0 0 0 0 0 2 0 0 0 0 0 0 1 4
Nuclear Periphery 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 1 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1
Vac/Vac Membrane 0 2 0 0 2 0 0 4 0 0 1 3 1 0 1 0 4 7 0 4 1 2 2 2
Unique Cell Count 259 416 151 111 119 171 182 256 207 198 179 218 123 174 141 133 277 300 255 405 150 126 125 135
Labelled Cell Count 259 419 155 112 124 174 194 269 227 219 198 246 124 175 142 146 295 318 255 405 150 126 125 135


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 116.8 76.2 93.1 83.0 52.7 48.7 42.1 39.8 36.3 36.0 34.9 32.3 89.5 85.7 80.4 92.0 128.0 120.8 88.6 105.3 96.5
Std Deviation (1e-4) 18.9 18.4 18.5 12.9 14.0 9.4 8.5 8.0 7.9 8.1 7.7 7.6 26.9 22.0 22.0 31.6 34.9 38.1 36.3 39.0 48.0
Intensity Change (Log2) – – – -0.17 -0.82 -0.94 -1.14 -1.23 -1.36 -1.37 -1.42 -1.53 -0.06 -0.12 -0.21 -0.02 0.46 0.38 -0.07 0.18 0.05


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 4.0 0 3.3 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 1.0 0.9 1.8 1.2 2.5 1.5 0 0 0 0 0 0 0
Cytoplasm -1.2 -3.2 -4.7 -5.4 -6.4 -6.4 -7.4 -6.5 -7.1 -2.2 -1.6 -1.8 -1.5 -1.8 -1.7
Endoplasmic Reticulum 0 0 3.5 4.4 5.0 4.7 5.3 5.1 4.5 0 0 0 3.6 0 2.8
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 3.4 3.9 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 3.5 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 112.8906 126.7589 122.3481 121.8153 118.0642 123.9422 118.0374 138.1038 128.2314 116.6341 122.3027 125.2605 101.1649 131.1833 116.7425 122.9433 101.3739 123.2304
Actin 0.0022 0.0002 0.0003 0.0003 0.0003 0.0003 0.0007 0.0003 0.0004 0.0008 0.0003 0.0015 0.0101 0.0002 0.0038 0.0001 0.0088 0.0002
Bud 0.0009 0.0007 0.001 0.0014 0.0012 0.0007 0.0009 0.0008 0.001 0.0022 0.0008 0.0016 0.0025 0.0003 0.0013 0.0012 0.0029 0.0006
Bud Neck 0.0009 0.0007 0.0011 0.0012 0.0011 0.0029 0.0008 0.0008 0.0009 0.0019 0.0011 0.0024 0.0005 0.0004 0.0022 0.0006 0.0009 0.0016
Bud Periphery 0.0014 0.0006 0.0012 0.0008 0.0009 0.0008 0.0017 0.0009 0.0007 0.0064 0.0008 0.0017 0.0046 0.0002 0.0015 0.0008 0.0158 0.0014
Bud Site 0.0007 0.0005 0.0005 0.0004 0.0001 0.0002 0.0004 0.0005 0.0003 0.001 0.0001 0.0001 0.0007 0.0002 0.0004 0.0001 0.0006 0.0001
Cell Periphery 0.0432 0.03 0.0313 0.0275 0.0108 0.0255 0.0368 0.0142 0.018 0.0642 0.009 0.0039 0.0189 0.0086 0.0074 0.0069 0.0084 0.008
Cytoplasm 0.5812 0.803 0.7382 0.562 0.7529 0.726 0.6851 0.8866 0.7891 0.6539 0.7582 0.8251 0.7422 0.9292 0.8386 0.823 0.8259 0.8689
Cytoplasmic Foci 0.0015 0.0009 0.0013 0.0027 0.0015 0.0021 0.0015 0.0015 0.0018 0.0014 0.0029 0.0047 0.0069 0.0006 0.005 0.0019 0.0245 0.0029
Eisosomes 0.0002 0 0 0.0005 0 0 0.0005 0 0 0 0 0 0.0003 0 0.0001 0 0.0001 0
Endoplasmic Reticulum 0.0841 0.0321 0.0168 0.0123 0.0172 0.08 0.0955 0.0348 0.0332 0.0234 0.0298 0.0598 0.0523 0.0112 0.012 0.002 0.0058 0.0244
Endosome 0.0073 0.0029 0.0054 0.0047 0.0067 0.0103 0.0066 0.004 0.0068 0.008 0.0134 0.0198 0.0092 0.0013 0.0042 0.0093 0.0069 0.0094
Golgi 0.0012 0.0004 0.0005 0.0002 0.0003 0.0009 0.0015 0.0006 0.0008 0.0006 0.0008 0.0118 0.0017 0.0002 0.0008 0.0003 0.0073 0.0006
Lipid Particles 0.002 0.0014 0.0014 0.0034 0.0007 0.0021 0.0015 0.001 0.0013 0.0023 0.0009 0.0006 0.0037 0.0006 0.001 0.0011 0.0021 0.0016
Mitochondria 0.0052 0.0004 0.0009 0.0013 0.0002 0.001 0.0053 0.0012 0.0016 0.0015 0.0012 0.0104 0.0051 0.0015 0.0044 0.0008 0.019 0.0017
None 0.0014 0.0002 0.0003 0.0013 0.0002 0.0003 0.0016 0.0002 0.0003 0.0003 0.0003 0.0005 0.0032 0.0002 0.0026 0.0002 0.0027 0.0004
Nuclear Periphery 0.0443 0.0095 0.0124 0.0101 0.0106 0.0121 0.0154 0.0033 0.0076 0.0129 0.0097 0.0064 0.0148 0.0019 0.0041 0.003 0.0022 0.0073
Nucleolus 0.0018 0.0003 0.0004 0.0087 0.0002 0.0004 0.0021 0.0002 0.0007 0.0008 0.0008 0.0001 0.0026 0.0001 0.0035 0.0002 0.0009 0.0001
Nucleus 0.0077 0.0024 0.0035 0.0061 0.0042 0.0032 0.0047 0.0015 0.0027 0.0052 0.0049 0.0018 0.0049 0.0012 0.0039 0.0019 0.0018 0.0021
Peroxisomes 0.0001 0 0.0001 0.0002 0 0 0.0001 0 0 0.0001 0 0.0001 0.0022 0 0.0003 0 0.0023 0
Punctate Nuclear 0.0001 0 0 0.0001 0 0 0.0001 0 0 0 0.0001 0 0.0004 0 0.0004 0 0.0003 0
Vacuole 0.1753 0.1044 0.1582 0.3135 0.1601 0.1062 0.1188 0.0432 0.1166 0.1839 0.1348 0.0304 0.0977 0.0393 0.0888 0.1297 0.0548 0.0559
Vacuole Periphery 0.0372 0.0092 0.0251 0.0414 0.0307 0.0249 0.0183 0.0044 0.016 0.0291 0.0301 0.0172 0.0158 0.0026 0.0137 0.0169 0.0061 0.0127

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 738.4915 800.4623 886.4956 839.5007 793.9523 673.6087 714.9229 605.5788 597.2618 686.7503
Translational Efficiency 2.0398 2.1298 2.0407 1.6707 2.2409 2.2343 1.9213 2.3363 2.2852 2.2483

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘
Standard name
Human Ortholog
Description Protein component of the small (40S) ribosomal subunit; interacts with Kti11p; deletion causes hypersensitivity to zymocin; homologous to mammalian ribosomal protein S7, no bacterial homolog; RPS7B has a paralog, RPS7A, that arose from the whole genome duplication; protein abundance increases in response to DNA replication stress
Localization
Cell Percentages cytoplasm (100%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-2

Rps7b

Rps7b


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Rps7b-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available