Standard name | |
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Human Ortholog | |
Description | Alpha-isopropylmalate synthase (2-isopropylmalate synthase); the main isozyme responsible for the first step in the leucine biosynthesis pathway; LEU4 has a paralog, LEU9, that arose from the whole genome duplication |
ensLOC | DeepLoc | |||||||||||||||||||||||
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Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 7 | 1 | 9 | 8 | 6 | 2 | 7 | – | 7 | 1 | 1 | 7 | – | 2 | 4 | 0 | 0 | 0 | 47 | 31 | 22 | 1 | 7 | 5 |
Bud | 3 | 1 | 0 | 1 | 0 | 1 | 2 | – | 0 | 1 | 0 | 4 | – | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 1 | 1 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 3 | 6 | 4 | 2 | 1 | 1 | 2 | – | 0 | 0 | 0 | 1 | – | 0 | 0 | 10 | 8 | 9 | 0 | 1 | 0 | 0 | 0 | 0 |
Cytoplasm | 1 | 3 | 3 | 1 | 5 | 4 | 4 | – | 4 | 4 | 1 | 2 | – | 8 | 2 | 8 | 12 | 9 | 0 | 0 | 0 | 0 | 0 | 0 |
Endoplasmic Reticulum | 7 | 1 | 0 | 0 | 0 | 0 | 1 | – | 1 | 0 | 1 | 1 | – | 0 | 1 | 19 | 29 | 29 | 2 | 1 | 0 | 0 | 0 | 0 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 4 | 4 | 5 | 0 | 0 | 0 |
Golgi | 99 | 79 | 106 | 65 | 93 | 37 | 44 | – | 40 | 30 | 16 | 37 | – | 53 | 38 | 196 | 125 | 119 | 193 | 137 | 54 | 13 | 32 | 30 |
Mitochondria | 279 | 139 | 73 | 114 | 126 | 201 | 204 | – | 198 | 150 | 176 | 182 | – | 34 | 32 | 85 | 86 | 53 | 157 | 99 | 99 | 12 | 20 | 26 |
Nucleus | 1 | 2 | 0 | 0 | 0 | 0 | 2 | – | 0 | 0 | 0 | 0 | – | 0 | 0 | 2 | 4 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 3 | – | 1 | 1 | 3 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 |
Nucleolus | 101 | 60 | 19 | 14 | 25 | 56 | 62 | – | 40 | 26 | 33 | 39 | – | 8 | 3 | 54 | 44 | 43 | 9 | 9 | 4 | 1 | 3 | 1 |
Peroxisomes | 0 | 0 | 2 | 2 | 2 | 0 | 0 | – | 0 | 0 | 0 | 1 | – | 0 | 0 | 0 | 1 | 0 | 17 | 11 | 13 | 0 | 1 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 1 | – | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 0 |
Vac/Vac Membrane | 36 | 38 | 34 | 4 | 19 | 32 | 24 | – | 18 | 15 | 9 | 14 | – | 7 | 2 | 32 | 31 | 18 | 4 | 7 | 3 | 2 | 0 | 1 |
Unique Cell Count | 387 | 244 | 181 | 147 | 198 | 255 | 261 | 249 | 174 | 192 | 224 | 81 | 55 | 326 | 280 | 230 | 452 | 319 | 210 | 38 | 71 | 69 | ||
Labelled Cell Count | 537 | 330 | 250 | 211 | 277 | 334 | 356 | 309 | 228 | 240 | 288 | 112 | 82 | 406 | 341 | 282 | 452 | 319 | 210 | 38 | 71 | 69 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 80.0 | 103.3 | 67.7 | 67.4 | 64.7 | 59.7 | 50.3 | – | 46.3 | 43.7 | 42.8 | 43.6 | – | 64.5 | 58.6 | 142.1 | 152.4 | 155.5 | – | 62.6 | 63.8 |
Std Deviation (1e-4) | 20.1 | 25.9 | 20.5 | 17.9 | 19.8 | 16.4 | 20.0 | – | 13.4 | 14.7 | 12.4 | 13.7 | – | 22.5 | 16.9 | 40.0 | 44.2 | 42.6 | – | 21.5 | 19.2 |
Intensity Change (Log2) | – | – | – | -0.01 | -0.07 | -0.18 | -0.43 | – | -0.55 | -0.63 | -0.66 | -0.63 | – | -0.07 | -0.21 | 1.07 | 1.17 | 1.2 | – | -0.11 | -0.09 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0.2 | -1.0 | 0 | -1.3 | – | -1.2 | 0 | 0 | -0.9 | – | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 1.0 |
Cytoplasm | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 1.6 | 1.3 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | – | 0 | 0 | 3.3 | 4.5 | 5.0 |
Endosome | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 |
Golgi | -2.6 | -2.3 | -9.7 | -9.1 | – | -9.2 | -8.0 | -10.3 | -8.8 | – | 1.1 | 1.4 | 0.3 | -2.9 | -1.4 |
Mitochondria | 6.8 | 4.5 | 8.2 | 8.1 | – | 8.3 | 8.9 | 10.5 | 8.5 | – | 0.3 | 2.3 | -3.3 | -2.1 | -3.8 |
Nucleus | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 |
Nucleolus | -0.3 | 0.6 | 3.1 | 3.5 | – | 1.7 | 1.3 | 1.9 | 2.0 | – | -0.2 | -1.1 | 1.9 | 1.6 | 2.3 |
Peroxisomes | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 |
Vacuole | -4.5 | -2.6 | -1.8 | -2.9 | – | -3.6 | -2.8 | -4.3 | -3.9 | – | -2.1 | -2.7 | -2.9 | -2.3 | -3.3 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 99.9994 | 114.9235 | 109.0189 | 96.51 | 101.2685 | 107.102 | 121.0375 | 126.886 | 125.6926 | 89.897 | 109.2972 | 118.571 | 148.0646 | 138.9156 | 136.5635 | 126.8661 | 123.9034 | 130.1539 |
Actin | 0.0176 | 0.0083 | 0.0051 | 0.0432 | 0.0007 | 0.0007 | 0.0185 | 0.0059 | 0.003 | 0.0465 | 0.0277 | 0.0047 | 0.0021 | 0.0074 | 0.0175 | 0.0013 | 0.038 | 0.0023 |
Bud | 0.0002 | 0.0002 | 0 | 0.0001 | 0.0001 | 0 | 0.0001 | 0.0002 | 0 | 0.0004 | 0.0001 | 0 | 0.0003 | 0 | 0.0001 | 0 | 0 | 0 |
Bud Neck | 0.0009 | 0.0005 | 0.0002 | 0.0003 | 0 | 0 | 0.0005 | 0.0018 | 0.0001 | 0.0023 | 0.0006 | 0.0003 | 0.0001 | 0.0001 | 0.0004 | 0 | 0.0001 | 0.0002 |
Bud Periphery | 0.0002 | 0.0003 | 0.0001 | 0.0001 | 0.0001 | 0 | 0.0002 | 0.0003 | 0.0001 | 0.0008 | 0.0002 | 0.0001 | 0.0002 | 0 | 0.0002 | 0 | 0.0001 | 0 |
Bud Site | 0.0009 | 0.0011 | 0.0002 | 0.0008 | 0.0002 | 0 | 0.0006 | 0.0027 | 0.0003 | 0.0023 | 0.0003 | 0.0001 | 0.0002 | 0.0001 | 0.001 | 0 | 0.0001 | 0.0001 |
Cell Periphery | 0.0005 | 0.003 | 0.0018 | 0.0013 | 0.0005 | 0.0006 | 0.0014 | 0.001 | 0.0008 | 0.0024 | 0.0006 | 0.0008 | 0.0004 | 0.0004 | 0.0003 | 0.0001 | 0.0003 | 0.0002 |
Cytoplasm | 0.0011 | 0.0001 | 0.0001 | 0 | 0.0001 | 0.0166 | 0.001 | 0 | 0 | 0.0054 | 0.0001 | 0 | 0.0006 | 0 | 0.0001 | 0 | 0 | 0 |
Cytoplasmic Foci | 0.0189 | 0.0012 | 0.0038 | 0.0002 | 0.0043 | 0.008 | 0.0038 | 0.0008 | 0.0003 | 0.0159 | 0.0007 | 0.0002 | 0.001 | 0.0001 | 0.0043 | 0 | 0.001 | 0.0048 |
Eisosomes | 0.0101 | 0.0086 | 0.0108 | 0.0026 | 0.0025 | 0.0011 | 0.0188 | 0.0081 | 0.0115 | 0.0083 | 0.0108 | 0.0028 | 0.0152 | 0.0028 | 0.0177 | 0.0011 | 0.0043 | 0.0043 |
Endoplasmic Reticulum | 0.0011 | 0 | 0 | 0 | 0 | 0.0003 | 0.0019 | 0 | 0 | 0.004 | 0.0004 | 0.0001 | 0.0001 | 0 | 0.0001 | 0 | 0 | 0 |
Endosome | 0.0115 | 0.0012 | 0.0004 | 0.0002 | 0.0002 | 0.0026 | 0.0026 | 0.0007 | 0.0001 | 0.0257 | 0.0015 | 0.0004 | 0.0002 | 0.0002 | 0.0017 | 0 | 0.0003 | 0.0015 |
Golgi | 0.0106 | 0.0068 | 0.0025 | 0.0011 | 0.001 | 0.0104 | 0.0046 | 0.0027 | 0.001 | 0.0177 | 0.0039 | 0.0111 | 0.0028 | 0.0048 | 0.0023 | 0 | 0.0018 | 0.0031 |
Lipid Particles | 0.0242 | 0.0194 | 0.0165 | 0.0162 | 0.0215 | 0.0154 | 0.0066 | 0.0083 | 0.0028 | 0.053 | 0.0062 | 0.0081 | 0.0026 | 0.007 | 0.0097 | 0.0008 | 0.0174 | 0.0023 |
Mitochondria | 0.844 | 0.9211 | 0.9186 | 0.9022 | 0.9346 | 0.8702 | 0.8982 | 0.9443 | 0.9631 | 0.7083 | 0.9212 | 0.9485 | 0.9577 | 0.9557 | 0.9226 | 0.9944 | 0.8912 | 0.9719 |
None | 0.0089 | 0 | 0.001 | 0 | 0.001 | 0.0002 | 0.0023 | 0 | 0.0001 | 0.0108 | 0.0007 | 0 | 0.0003 | 0 | 0.0005 | 0 | 0.0002 | 0 |
Nuclear Periphery | 0.0041 | 0.0001 | 0.0003 | 0.0001 | 0.0003 | 0.0006 | 0.0037 | 0.0001 | 0.0001 | 0.0127 | 0.0026 | 0.0005 | 0.0006 | 0.0001 | 0.0004 | 0 | 0.0002 | 0.0001 |
Nucleolus | 0.0206 | 0.0019 | 0.0077 | 0.0016 | 0.0026 | 0.0093 | 0.0138 | 0.0044 | 0.0051 | 0.0142 | 0.0007 | 0.0036 | 0.007 | 0.0006 | 0.0037 | 0.0002 | 0.0294 | 0.0001 |
Nucleus | 0.0024 | 0.0003 | 0.0005 | 0.0002 | 0.0003 | 0.0004 | 0.0018 | 0.0004 | 0.0004 | 0.0073 | 0.0004 | 0.0003 | 0.0018 | 0.0001 | 0.0002 | 0 | 0.0009 | 0 |
Peroxisomes | 0.0053 | 0.0042 | 0.0039 | 0.0014 | 0.0067 | 0.0009 | 0.005 | 0.0064 | 0.0016 | 0.0075 | 0.0026 | 0.001 | 0.0022 | 0.0013 | 0.0104 | 0.0013 | 0.0026 | 0.0027 |
Punctate Nuclear | 0.0035 | 0 | 0.0001 | 0 | 0 | 0 | 0.0055 | 0 | 0 | 0.0156 | 0.0027 | 0 | 0.0001 | 0 | 0.0007 | 0 | 0.0003 | 0.0001 |
Vacuole | 0.0019 | 0.004 | 0.0021 | 0.0016 | 0.001 | 0.0027 | 0.0015 | 0.0018 | 0.0008 | 0.0052 | 0.0008 | 0.0006 | 0.0004 | 0.0009 | 0.0005 | 0.0001 | 0.0008 | 0.0003 |
Vacuole Periphery | 0.0117 | 0.0177 | 0.0243 | 0.0266 | 0.0223 | 0.0597 | 0.0077 | 0.01 | 0.0087 | 0.0338 | 0.0152 | 0.017 | 0.0042 | 0.0182 | 0.0054 | 0.0006 | 0.0109 | 0.006 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 186.5703 | 152.7292 | 123.9565 | 116.2126 | 144.5995 | 203.9575 | 182.8427 | 153.2629 | 163.971 | 203.4264 |
Translational Efficiency | 1.2165 | 1.1107 | 0.9846 | 1.2723 | 1.2403 | 1.2733 | 1.0662 | 1.2061 | 1.1465 | 1.0799 |
Dataset | Hit |
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Protein Concentration | ✔ |
Protein Localization | ✘ |
Gene Expression | ✔ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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